Click here to switch to the map view.

The map label for this gene is dnaK [H]

Identifier: 86608970

GI number: 86608970

Start: 1577051

End: 1578643

Strand: Reverse

Name: dnaK [H]

Synonym: CYB_1503

Alternate gene names: 86608970

Gene position: 1578643-1577051 (Counterclockwise)

Preceding gene: 86608971

Following gene: 86608969

Centisome position: 51.82

GC content: 60.89

Gene sequence:

>1593_bases
ATGGCACCTGCCCCCAAAGCGACCCTAGCCATTGATTTTGGCACCAGCAACACGGTGGTGGCAGTTTGGGATCCCGCCCA
AGCAGTGCCCCGTCTCCTCACTCTTCCCGGCCTCTCTCGCCCAGACCACTCGGCTATTCCCAGCTTGGTCTACGTGAAGG
GAAAAGGCCAGCTCCTGGTGGGGGAAACCGTGCGCGCCGGTCGGTGGGGGGCAGTGGATCCGCAACGGCTGTTTCAGGGG
TTTAAGCGGGACTTGGTGGCGGAGTTTGTCCCTCCGCCGCGAGTTCTAGATGGAGAGCGCTACGACGCCGAGAGGGTGGC
CCAAGCTTTTTTGCAGACGGTGATGGAAGCTGTCCCGAAAGAACACCTGCAGCCGCAACAGCTGGTGTTCACCGCCCCTG
TCGGCTCTTTTGAGCGCTACCTCAATTGGCTGCGGGGAGTGGCCAGTGCCTGTGGCTGGGATCCCGTTCAAATTGTCGAT
GAAGCCACGGCAGCAGCCCTGGGCTATGCGGTTGGCCAGGCGGGATCCCTAGTGCTGGTGGTGGATTTCGGCGGCGGTAC
CTTGGATCTCAGCTTGGTGCGCACCCTCGCTCCCCAAGCGGGTAGCCCTGTCCTCAAAGCGGAGGTGATCGCCAAGGCCG
ATGCCTATGTGGGCGGGGTGGACATCGACATTTGGATTGCCGACTACCTACTGCAGCAACTGGGGCTAAGCCGACAGCAA
ATTGGAGCTCTGGGCTGGCTCAACCTCCTGGAGCAGGCGGAACAGCTCAAGATCGCCCTTTCCACTCAGATGGAAGCGGT
GGGCAGTTGGTTTGACGACGAAGCCCTCACTGCCCATGAGTTGAAGTTAAGCCGGCAGGAGCTGGAAGAGATCCTGGAAG
CTCGTCAGCTTTTGGATCAAATCCGCCAGTGCCTAGACGACGTTCTCCTCACCGCCCAGGCGAGGGGATTGGGCAAGAAC
CAAATTGAGCAGGTGCTCTTGGTAGGAGGCAGCTCCCAACTGCCAGCAGTTCAAGAGCTGCTGCGCTCCTACTTCGGCAA
AAAGAAAGTGCGCTGCGAACGCCCCTTCGATGCTGTGGCCCTGGGCGCCCTGCAGGTGGGCCGCCAGGTGAAGCTGGAGG
ATCGCCTGCACCACAGCTATGCCCTCCGCCTCTGGGATCCCTTGCAGAAAAGCTACATCTACTACCCCCTCTTCGAACAG
GGCAGCCCCTATCCCTGCCGACGGGCTGAGCCCTTGATCCTGCAAGTGGCCAACGACGGACAAACGGAGATTCGCCTGGA
GGTGGGCGAAGTGGCCCAGGTAGTCCAGTCGGAAGTGGTTTACGACGAGCTGGGGCGGATGAGCAGCCGGCAGCTCACCC
GGCAAACCGATTTCCGATCCCTAGCTCCTCTTGGATCCCCAACCGTTCGCGCTACTTCGACGGAGTCATCACCAATTTGT
CTGGCCCGTTTGCAGCCCCCTGGCCGCGTGGGAGAGGATCGCCTTCGCGTCGAATTTGCCATTGATAGCTCGCGGCGCTT
GTTGGTCACCATCACCGACCTGCAAACTCAGGCGCTCTTGGCAGAGGCCCAGCCTGTAGCCACCTTGAGTTAA

Upstream 100 bases:

>100_bases
AGGGGGATCCCGTTTACATTCGCTTTGTCGGCTACCGCCGCGGTGACCACATCCTCTGCCCAGCCAAAGTCAGCCGTTCC
CTGCCTGGAGGATTGAGCTG

Downstream 100 bases:

>100_bases
GAGACGAAAGTAGAGAGAAGAGAGCGTCTTCACTGCAACGGAAGACAAAGAAAGCAATGTAAAGGGCACGACATGGCCAC
GATTCCCAACTCTCATCTCC

Product: DnaK family protein

Products: NA

Alternate protein names: HSP70; Heat shock 70 kDa protein; Heat shock protein 70 [H]

Number of amino acids: Translated: 530; Mature: 529

Protein sequence:

>530_residues
MAPAPKATLAIDFGTSNTVVAVWDPAQAVPRLLTLPGLSRPDHSAIPSLVYVKGKGQLLVGETVRAGRWGAVDPQRLFQG
FKRDLVAEFVPPPRVLDGERYDAERVAQAFLQTVMEAVPKEHLQPQQLVFTAPVGSFERYLNWLRGVASACGWDPVQIVD
EATAAALGYAVGQAGSLVLVVDFGGGTLDLSLVRTLAPQAGSPVLKAEVIAKADAYVGGVDIDIWIADYLLQQLGLSRQQ
IGALGWLNLLEQAEQLKIALSTQMEAVGSWFDDEALTAHELKLSRQELEEILEARQLLDQIRQCLDDVLLTAQARGLGKN
QIEQVLLVGGSSQLPAVQELLRSYFGKKKVRCERPFDAVALGALQVGRQVKLEDRLHHSYALRLWDPLQKSYIYYPLFEQ
GSPYPCRRAEPLILQVANDGQTEIRLEVGEVAQVVQSEVVYDELGRMSSRQLTRQTDFRSLAPLGSPTVRATSTESSPIC
LARLQPPGRVGEDRLRVEFAIDSSRRLLVTITDLQTQALLAEAQPVATLS

Sequences:

>Translated_530_residues
MAPAPKATLAIDFGTSNTVVAVWDPAQAVPRLLTLPGLSRPDHSAIPSLVYVKGKGQLLVGETVRAGRWGAVDPQRLFQG
FKRDLVAEFVPPPRVLDGERYDAERVAQAFLQTVMEAVPKEHLQPQQLVFTAPVGSFERYLNWLRGVASACGWDPVQIVD
EATAAALGYAVGQAGSLVLVVDFGGGTLDLSLVRTLAPQAGSPVLKAEVIAKADAYVGGVDIDIWIADYLLQQLGLSRQQ
IGALGWLNLLEQAEQLKIALSTQMEAVGSWFDDEALTAHELKLSRQELEEILEARQLLDQIRQCLDDVLLTAQARGLGKN
QIEQVLLVGGSSQLPAVQELLRSYFGKKKVRCERPFDAVALGALQVGRQVKLEDRLHHSYALRLWDPLQKSYIYYPLFEQ
GSPYPCRRAEPLILQVANDGQTEIRLEVGEVAQVVQSEVVYDELGRMSSRQLTRQTDFRSLAPLGSPTVRATSTESSPIC
LARLQPPGRVGEDRLRVEFAIDSSRRLLVTITDLQTQALLAEAQPVATLS
>Mature_529_residues
APAPKATLAIDFGTSNTVVAVWDPAQAVPRLLTLPGLSRPDHSAIPSLVYVKGKGQLLVGETVRAGRWGAVDPQRLFQGF
KRDLVAEFVPPPRVLDGERYDAERVAQAFLQTVMEAVPKEHLQPQQLVFTAPVGSFERYLNWLRGVASACGWDPVQIVDE
ATAAALGYAVGQAGSLVLVVDFGGGTLDLSLVRTLAPQAGSPVLKAEVIAKADAYVGGVDIDIWIADYLLQQLGLSRQQI
GALGWLNLLEQAEQLKIALSTQMEAVGSWFDDEALTAHELKLSRQELEEILEARQLLDQIRQCLDDVLLTAQARGLGKNQ
IEQVLLVGGSSQLPAVQELLRSYFGKKKVRCERPFDAVALGALQVGRQVKLEDRLHHSYALRLWDPLQKSYIYYPLFEQG
SPYPCRRAEPLILQVANDGQTEIRLEVGEVAQVVQSEVVYDELGRMSSRQLTRQTDFRSLAPLGSPTVRATSTESSPICL
ARLQPPGRVGEDRLRVEFAIDSSRRLLVTITDLQTQALLAEAQPVATLS

Specific function: Acts as a chaperone [H]

COG id: COG0443

COG function: function code O; Molecular chaperone

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the heat shock protein 70 family [H]

Homologues:

Organism=Homo sapiens, GI24234688, Length=464, Percent_Identity=24.3534482758621, Blast_Score=99, Evalue=9e-21,
Organism=Homo sapiens, GI16507237, Length=232, Percent_Identity=29.3103448275862, Blast_Score=86, Evalue=7e-17,
Organism=Homo sapiens, GI34419635, Length=271, Percent_Identity=28.7822878228782, Blast_Score=83, Evalue=8e-16,
Organism=Homo sapiens, GI13676857, Length=339, Percent_Identity=25.3687315634218, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI5729877, Length=271, Percent_Identity=26.9372693726937, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI24234686, Length=271, Percent_Identity=27.6752767527675, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI82880658, Length=311, Percent_Identity=26.3665594855305, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1786196, Length=388, Percent_Identity=26.5463917525773, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1786870, Length=415, Percent_Identity=25.3012048192771, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788875, Length=392, Percent_Identity=26.2755102040816, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17534771, Length=396, Percent_Identity=26.7676767676768, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17507981, Length=284, Percent_Identity=28.8732394366197, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17568549, Length=282, Percent_Identity=28.7234042553192, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17534015, Length=248, Percent_Identity=30.241935483871, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17534013, Length=248, Percent_Identity=30.241935483871, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17562024, Length=471, Percent_Identity=25.2653927813163, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71984444, Length=474, Percent_Identity=25.1054852320675, Blast_Score=79, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6322505, Length=393, Percent_Identity=26.4631043256997, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6320805, Length=467, Percent_Identity=26.5524625267666, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6322426, Length=334, Percent_Identity=29.3413173652695, Blast_Score=93, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6323004, Length=244, Percent_Identity=29.0983606557377, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6324120, Length=254, Percent_Identity=29.5275590551181, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6319972, Length=254, Percent_Identity=29.5275590551181, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6319396, Length=245, Percent_Identity=29.7959183673469, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6319314, Length=244, Percent_Identity=29.0983606557377, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6320950, Length=245, Percent_Identity=28.9795918367347, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6325151, Length=339, Percent_Identity=24.188790560472, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323401, Length=499, Percent_Identity=22.8456913827655, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24653595, Length=463, Percent_Identity=25.2699784017279, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI17738165, Length=353, Percent_Identity=26.3456090651558, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24641406, Length=390, Percent_Identity=25.3846153846154, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24641408, Length=390, Percent_Identity=25.3846153846154, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24641402, Length=390, Percent_Identity=25.3846153846154, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24641404, Length=390, Percent_Identity=25.3846153846154, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI17737941, Length=392, Percent_Identity=26.530612244898, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24646324, Length=248, Percent_Identity=29.0322580645161, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24646322, Length=248, Percent_Identity=29.0322580645161, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24646318, Length=248, Percent_Identity=29.0322580645161, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI28571678, Length=248, Percent_Identity=29.0322580645161, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24646137, Length=323, Percent_Identity=26.625386996904, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24646135, Length=323, Percent_Identity=26.625386996904, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24647034, Length=247, Percent_Identity=28.3400809716599, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24647038, Length=247, Percent_Identity=28.3400809716599, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24647036, Length=247, Percent_Identity=28.3400809716599, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI17737967, Length=247, Percent_Identity=28.3400809716599, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI28571721, Length=247, Percent_Identity=28.3400809716599, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI28571719, Length=247, Percent_Identity=28.3400809716599, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: 1300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 320 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 500 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stati

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012725
- InterPro:   IPR018181
- InterPro:   IPR001023
- InterPro:   IPR013126 [H]

Pfam domain/function: PF00012 HSP70 [H]

EC number: NA

Molecular weight: Translated: 58092; Mature: 57961

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00329 HSP70_2 ; PS01036 HSP70_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPAPKATLAIDFGTSNTVVAVWDPAQAVPRLLTLPGLSRPDHSAIPSLVYVKGKGQLLV
CCCCCCEEEEEEECCCCCEEEEECCHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCEEE
GETVRAGRWGAVDPQRLFQGFKRDLVAEFVPPPRVLDGERYDAERVAQAFLQTVMEAVPK
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCH
EHLQPQQLVFTAPVGSFERYLNWLRGVASACGWDPVQIVDEATAAALGYAVGQAGSLVLV
HHCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEE
VDFGGGTLDLSLVRTLAPQAGSPVLKAEVIAKADAYVGGVDIDIWIADYLLQQLGLSRQQ
EECCCCEEHHHHHHHHCCCCCCCCHHHHHHHHHCCEECCCEEHHHHHHHHHHHHCCCHHH
IGALGWLNLLEQAEQLKIALSTQMEAVGSWFDDEALTAHELKLSRQELEEILEARQLLDQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
IRQCLDDVLLTAQARGLGKNQIEQVLLVGGSSQLPAVQELLRSYFGKKKVRCERPFDAVA
HHHHHHHHHHHHHHCCCCHHHHHEEEEECCCCCCHHHHHHHHHHCCCCCEECCCCHHHHH
LGALQVGRQVKLEDRLHHSYALRLWDPLQKSYIYYPLFEQGSPYPCRRAEPLILQVANDG
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCCCCCEEEEEECCC
QTEIRLEVGEVAQVVQSEVVYDELGRMSSRQLTRQTDFRSLAPLGSPTVRATSTESSPIC
CEEEEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHCCCCCCCEEEECCCCCCEE
LARLQPPGRVGEDRLRVEFAIDSSRRLLVTITDLQTQALLAEAQPVATLS
EEEECCCCCCCCCEEEEEEEECCCCEEEEEEECCHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
APAPKATLAIDFGTSNTVVAVWDPAQAVPRLLTLPGLSRPDHSAIPSLVYVKGKGQLLV
CCCCCEEEEEEECCCCCEEEEECCHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCEEE
GETVRAGRWGAVDPQRLFQGFKRDLVAEFVPPPRVLDGERYDAERVAQAFLQTVMEAVPK
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCH
EHLQPQQLVFTAPVGSFERYLNWLRGVASACGWDPVQIVDEATAAALGYAVGQAGSLVLV
HHCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEE
VDFGGGTLDLSLVRTLAPQAGSPVLKAEVIAKADAYVGGVDIDIWIADYLLQQLGLSRQQ
EECCCCEEHHHHHHHHCCCCCCCCHHHHHHHHHCCEECCCEEHHHHHHHHHHHHCCCHHH
IGALGWLNLLEQAEQLKIALSTQMEAVGSWFDDEALTAHELKLSRQELEEILEARQLLDQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
IRQCLDDVLLTAQARGLGKNQIEQVLLVGGSSQLPAVQELLRSYFGKKKVRCERPFDAVA
HHHHHHHHHHHHHHCCCCHHHHHEEEEECCCCCCHHHHHHHHHHCCCCCEECCCCHHHHH
LGALQVGRQVKLEDRLHHSYALRLWDPLQKSYIYYPLFEQGSPYPCRRAEPLILQVANDG
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCCCCCEEEEEECCC
QTEIRLEVGEVAQVVQSEVVYDELGRMSSRQLTRQTDFRSLAPLGSPTVRATSTESSPIC
CEEEEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHCCCCCCCEEEECCCCCCEE
LARLQPPGRVGEDRLRVEFAIDSSRRLLVTITDLQTQALLAEAQPVATLS
EEEECCCCCCCCCEEEEEEEECCCCEEEEEEECCHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA