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The map label for this gene is pyrE [H]

Identifier: 86608954

GI number: 86608954

Start: 1562864

End: 1563481

Strand: Direct

Name: pyrE [H]

Synonym: CYB_1487

Alternate gene names: 86608954

Gene position: 1562864-1563481 (Clockwise)

Preceding gene: 86608953

Following gene: 86608955

Centisome position: 51.3

GC content: 61.17

Gene sequence:

>618_bases
GTGTTGCTTCTCGATTGCTCTCCCCTGACCCTTTCCCCAGAGGAAGCGCGGGAGAAACTGTTGTATCTCCTCGCCCAACT
GGCCTATCGAGAAGGCTCCTTTACCCTCACTTCTGGACAGAAGAGCCCCTACTACATCAACTGCAAACCGGTTACCCTTC
ACCCCCAAGGAGCTTATTGGGTGGGATCCCTGCTGCACCGACTGTTGCCGCCCGAAACCGAGGCAGTGGCCGGAATGACG
CTGGGGGCAGATCCCCTGGTGACGGCGGTGAGTTTGGCCTCCCTGAGGGCTGAGCCTGCCAACCTAGCCGCCCTAATCGT
GCGCAAAGAGCCCAAGGGGCACGGCACGCAAGCCTGGATCGAGGGGCCGGAGTTGCCGGCAGGCAGTCGGGTGTGGATCC
TGGAAGATGTGGTGACCACCGGCAGTTCTGCTTTGAAGGCTGTCGAACGGGTGCGGGCAGCCGGCTACCGGGCGGAAGGG
ATCCTGGCTTTGGTGGATCGGCTGGAAGGGGCAGAAGACCGCTGCCGAGAAGCAGGGATCCCCTTTCGTCGCCTATTCAC
CCTTCCAGAAGTGCAGGCTTGCCATCGGGCCTTGACCTCAGGAAAATCAAAGGACTAG

Upstream 100 bases:

>100_bases
AGCCGAAAAACGGGAGATTGGATTCGGTGGAGATTTGCCGCATAATAAAATGTCCTGAGTGCTCTTTCTGGCCAGCCATT
GAACCAAGGGGATCCCTGCT

Downstream 100 bases:

>100_bases
CCTGCCCCCTTGGTGAGCATCAAAGCTTTGGAGAGCGGACATGCTGCCCCCTGCCTACTCAGCTCTGCCCAGCCGAAATC
TGCCAAGCCTACCGGATCAG

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase [H]

Number of amino acids: Translated: 205; Mature: 205

Protein sequence:

>205_residues
MLLLDCSPLTLSPEEAREKLLYLLAQLAYREGSFTLTSGQKSPYYINCKPVTLHPQGAYWVGSLLHRLLPPETEAVAGMT
LGADPLVTAVSLASLRAEPANLAALIVRKEPKGHGTQAWIEGPELPAGSRVWILEDVVTTGSSALKAVERVRAAGYRAEG
ILALVDRLEGAEDRCREAGIPFRRLFTLPEVQACHRALTSGKSKD

Sequences:

>Translated_205_residues
MLLLDCSPLTLSPEEAREKLLYLLAQLAYREGSFTLTSGQKSPYYINCKPVTLHPQGAYWVGSLLHRLLPPETEAVAGMT
LGADPLVTAVSLASLRAEPANLAALIVRKEPKGHGTQAWIEGPELPAGSRVWILEDVVTTGSSALKAVERVRAAGYRAEG
ILALVDRLEGAEDRCREAGIPFRRLFTLPEVQACHRALTSGKSKD
>Mature_205_residues
MLLLDCSPLTLSPEEAREKLLYLLAQLAYREGSFTLTSGQKSPYYINCKPVTLHPQGAYWVGSLLHRLLPPETEAVAGMT
LGADPLVTAVSLASLRAEPANLAALIVRKEPKGHGTQAWIEGPELPAGSRVWILEDVVTTGSSALKAVERVRAAGYRAEG
ILALVDRLEGAEDRCREAGIPFRRLFTLPEVQACHRALTSGKSKD

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) [H]

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507835, Length=164, Percent_Identity=32.3170731707317, Blast_Score=83, Evalue=2e-16,
Organism=Escherichia coli, GI1790073, Length=157, Percent_Identity=28.0254777070064, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17508631, Length=167, Percent_Identity=30.5389221556886, Blast_Score=77, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17554964, Length=178, Percent_Identity=29.7752808988764, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI17933654, Length=181, Percent_Identity=30.3867403314917, Blast_Score=67, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.4.2.10 [H]

Molecular weight: Translated: 22214; Mature: 22214

Theoretical pI: Translated: 7.45; Mature: 7.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLLDCSPLTLSPEEAREKLLYLLAQLAYREGSFTLTSGQKSPYYINCKPVTLHPQGAYW
CEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEEEEEECCCCCHH
VGSLLHRLLPPETEAVAGMTLGADPLVTAVSLASLRAEPANLAALIVRKEPKGHGTQAWI
HHHHHHHHCCCCCHHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCEEEE
EGPELPAGSRVWILEDVVTTGSSALKAVERVRAAGYRAEGILALVDRLEGAEDRCREAGI
CCCCCCCCCEEEEEHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHCCC
PFRRLFTLPEVQACHRALTSGKSKD
CHHHHHCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MLLLDCSPLTLSPEEAREKLLYLLAQLAYREGSFTLTSGQKSPYYINCKPVTLHPQGAYW
CEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEEEEEECCCCCHH
VGSLLHRLLPPETEAVAGMTLGADPLVTAVSLASLRAEPANLAALIVRKEPKGHGTQAWI
HHHHHHHHCCCCCHHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCEEEE
EGPELPAGSRVWILEDVVTTGSSALKAVERVRAAGYRAEGILALVDRLEGAEDRCREAGI
CCCCCCCCCEEEEEHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHCCC
PFRRLFTLPEVQACHRALTSGKSKD
CHHHHHCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]