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The map label for this gene is pnpA [H]

Identifier: 86608913

GI number: 86608913

Start: 1514303

End: 1516441

Strand: Direct

Name: pnpA [H]

Synonym: CYB_1445

Alternate gene names: 86608913

Gene position: 1514303-1516441 (Clockwise)

Preceding gene: 86608912

Following gene: 86608914

Centisome position: 49.7

GC content: 60.54

Gene sequence:

>2139_bases
ATGGCAGAATACACTCGGTCGATTTCCTTTTATGGGCGGGAAATTGACATCAATATTGGGCTGATGGCCCCCCAGGCAGG
GTGTGGGGTTTGGCTGACTTCGGGCGAAACCTCGATTTTGGTTACCGCCACACGGCAGCCAGGCCGGCCTGGCGTTGATT
TTATGCCCTTGTTGGTCGATTACGAAGAGCGCCTCTACGCAGCGGGGCGGATCCCAGGGGGATATCTGCGGCGAGAAGGC
CGGCCACCGGAACGGGCCACTTTGATCTCTCGCTTAATAGACCGACCCATTCGTCCGTTGTTCCCGGAATGGCTGCGGGA
TGATGTGCAGGTGGTGGCCACCACCCTCTCGGTGGACGATACGGTGCCGCCCGATGTGTTGTGTATTTTGGGAGCCTCGT
TAGCCATTCACGGCGCTCGCATCCCCTTCAATGGCCCCGTGGCGGCGGTGCGGGTGGGCTTGGTCAAGGATGAATTTATC
CTCAACCCCACCTATGCCGAAATTGAGGCGGGCGATCTGGATCTGGTGGTGGCCGGCTGTGCCGATGGCGTGATCATGGT
GGAAGCCGGGGCCAACCAACTGCCGGAAAAGGATGTGGTGGAGGCCATCGAGTTTGGTTTTGAGGCCATCCAGGAGCTGC
TCAAAGCCCAACAGCAAGTCCTGGCGGATCTCAACATCACCCCGGTAGAGCTGCCCCCTCCCCCCAAAAACGAGGAGCTG
ATTGCCTTTGTAGAAGAGCAGGCTCAGGAGGGCATTCGCTCCATTCTCAGGCAGTTTTTGGACAAGACCAGCCGGGAACA
GCAACTGGAGGAGCTCAAGGCCAAGCTGGAGGCCCAAATCCAGGAACGGCCAGAGGGGGATCCCTTGCGGCTCTATCTTC
TGGAAAACCCCAAAGAGTTGGACAACCAGTTCAAGGCTCTGCTCAAGAAGCTGATGCGCCAGCAAATCCTGCAAGAGGGG
GTGCGGGTAGATGGACGCAAGCTGGACGAGGTGCGACCGGTCTCTTGCCGGGTGGGTTTGATCCCAAGGGTTCACGGCAG
TGCCTTGTTCAATCGCGGCCTCACCCAAGTCCTTTCCATCACTACCCTCGGCACGCCCGGCGATGCCCAGGAGCTGGACG
ACTTGCACCCGGTGGATGAGAAGCGCTACATGCACCACTACAATTTTCCGGGCTTTTCGGTGGGAGAAACGCGGCCTTCA
CGCTCGCCGGGACGGCGGGAGATTGGTCATGGGGCGCTGGCGGAGCGGGCCTTGGTGCCGGTGCTGCCCAAGGAAGAGGA
GTTTCCCTACGTGGTGAGGGTGGTGTCGGAAGTGCTCTCCTCCAACGGCTCCACCTCGATGGGATCCGTCTGTGGCTCTA
CCCTTTCTCTGATGGATGCCGGGGTGCCGATCAAAGCGCCGGTCAGCGGGGTGGCCATGGGCCTTATTAAGGAGGGGGAT
GAGGTGCGCATCCTCACCGACATCCAGGGGATCGAGGACTTTCTGGGCGACATGGACTTCAAGGTGGCCGGTACGCGGGC
GGGGATCACCGCTCTGCAAATGGACATGAAAATCACCGGCATCACCGTTGACGTGGTGGAACAGGCCATCCGGCAAGCCA
AGGCCGGACGCGAGTTCATCCTCGACAAGATGCTGGAGACGATTGCTGCGCCTCGACCCCAGTTGGCCAAAACGGCTCCC
CGTCTGCTCACGTTCAAGGTGGATCCCGAGGATATCGGCAAGATCATCGGCCCCGGTGGCAAGACCGTGCGCGGCATCAC
CGAAGCGACAGGGGCCAAGGTGGACATCAGCGACGACGGCACAATCACCGTGTCTTCCTCGGTGGGCGGCCAGGCGGAGG
CGGCGCGGGCGATGATCGAAAATCTGGTGCGGCGGGTGGAAGAGGGGCAGGTGTATTTGGGCAAGGTGACCCGCATCATC
CCCATCGGCGCTTTTGTGGAGTTTTTGCCCGGCAAAGAGGGCATGATCCACATTTCGCAACTGGCGGAGTACCGCGTCGG
CAGGGTGGAAGACGAGGTGGCTGTGGAAGATGAGGTGGTGGTCAAGGTGCGCAGCATCGATCACAAAGGCCGTATCAACC
TCACTCGCCTCGGCATTAGCCCTGAGGAAGCAGCGCGGGTGCGCAACCATCACCACTGA

Upstream 100 bases:

>100_bases
GAGACGAAGTCGTCCTGATCCCTCCGGTCAGCGGGGGCTGAAAAGGAGCAAAAGGAGAATGCTACGATAGCGGCAAGAAA
AAAAGAAGGCGAAGCGTTAC

Downstream 100 bases:

>100_bases
GGCTGCGGTTTTCCCCCAGCAACGGGGGGGCCAGGGATCCCCTCGACAGCCGCCGGCAGCGGCCAAAATGGCAGAGAAAA
CTCCATCGCGACAGCGGTGC

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 712; Mature: 711

Protein sequence:

>712_residues
MAEYTRSISFYGREIDINIGLMAPQAGCGVWLTSGETSILVTATRQPGRPGVDFMPLLVDYEERLYAAGRIPGGYLRREG
RPPERATLISRLIDRPIRPLFPEWLRDDVQVVATTLSVDDTVPPDVLCILGASLAIHGARIPFNGPVAAVRVGLVKDEFI
LNPTYAEIEAGDLDLVVAGCADGVIMVEAGANQLPEKDVVEAIEFGFEAIQELLKAQQQVLADLNITPVELPPPPKNEEL
IAFVEEQAQEGIRSILRQFLDKTSREQQLEELKAKLEAQIQERPEGDPLRLYLLENPKELDNQFKALLKKLMRQQILQEG
VRVDGRKLDEVRPVSCRVGLIPRVHGSALFNRGLTQVLSITTLGTPGDAQELDDLHPVDEKRYMHHYNFPGFSVGETRPS
RSPGRREIGHGALAERALVPVLPKEEEFPYVVRVVSEVLSSNGSTSMGSVCGSTLSLMDAGVPIKAPVSGVAMGLIKEGD
EVRILTDIQGIEDFLGDMDFKVAGTRAGITALQMDMKITGITVDVVEQAIRQAKAGREFILDKMLETIAAPRPQLAKTAP
RLLTFKVDPEDIGKIIGPGGKTVRGITEATGAKVDISDDGTITVSSSVGGQAEAARAMIENLVRRVEEGQVYLGKVTRII
PIGAFVEFLPGKEGMIHISQLAEYRVGRVEDEVAVEDEVVVKVRSIDHKGRINLTRLGISPEEAARVRNHHH

Sequences:

>Translated_712_residues
MAEYTRSISFYGREIDINIGLMAPQAGCGVWLTSGETSILVTATRQPGRPGVDFMPLLVDYEERLYAAGRIPGGYLRREG
RPPERATLISRLIDRPIRPLFPEWLRDDVQVVATTLSVDDTVPPDVLCILGASLAIHGARIPFNGPVAAVRVGLVKDEFI
LNPTYAEIEAGDLDLVVAGCADGVIMVEAGANQLPEKDVVEAIEFGFEAIQELLKAQQQVLADLNITPVELPPPPKNEEL
IAFVEEQAQEGIRSILRQFLDKTSREQQLEELKAKLEAQIQERPEGDPLRLYLLENPKELDNQFKALLKKLMRQQILQEG
VRVDGRKLDEVRPVSCRVGLIPRVHGSALFNRGLTQVLSITTLGTPGDAQELDDLHPVDEKRYMHHYNFPGFSVGETRPS
RSPGRREIGHGALAERALVPVLPKEEEFPYVVRVVSEVLSSNGSTSMGSVCGSTLSLMDAGVPIKAPVSGVAMGLIKEGD
EVRILTDIQGIEDFLGDMDFKVAGTRAGITALQMDMKITGITVDVVEQAIRQAKAGREFILDKMLETIAAPRPQLAKTAP
RLLTFKVDPEDIGKIIGPGGKTVRGITEATGAKVDISDDGTITVSSSVGGQAEAARAMIENLVRRVEEGQVYLGKVTRII
PIGAFVEFLPGKEGMIHISQLAEYRVGRVEDEVAVEDEVVVKVRSIDHKGRINLTRLGISPEEAARVRNHHH
>Mature_711_residues
AEYTRSISFYGREIDINIGLMAPQAGCGVWLTSGETSILVTATRQPGRPGVDFMPLLVDYEERLYAAGRIPGGYLRREGR
PPERATLISRLIDRPIRPLFPEWLRDDVQVVATTLSVDDTVPPDVLCILGASLAIHGARIPFNGPVAAVRVGLVKDEFIL
NPTYAEIEAGDLDLVVAGCADGVIMVEAGANQLPEKDVVEAIEFGFEAIQELLKAQQQVLADLNITPVELPPPPKNEELI
AFVEEQAQEGIRSILRQFLDKTSREQQLEELKAKLEAQIQERPEGDPLRLYLLENPKELDNQFKALLKKLMRQQILQEGV
RVDGRKLDEVRPVSCRVGLIPRVHGSALFNRGLTQVLSITTLGTPGDAQELDDLHPVDEKRYMHHYNFPGFSVGETRPSR
SPGRREIGHGALAERALVPVLPKEEEFPYVVRVVSEVLSSNGSTSMGSVCGSTLSLMDAGVPIKAPVSGVAMGLIKEGDE
VRILTDIQGIEDFLGDMDFKVAGTRAGITALQMDMKITGITVDVVEQAIRQAKAGREFILDKMLETIAAPRPQLAKTAPR
LLTFKVDPEDIGKIIGPGGKTVRGITEATGAKVDISDDGTITVSSSVGGQAEAARAMIENLVRRVEEGQVYLGKVTRIIP
IGAFVEFLPGKEGMIHISQLAEYRVGRVEDEVAVEDEVVVKVRSIDHKGRINLTRLGISPEEAARVRNHHH

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=716, Percent_Identity=37.5698324022346, Blast_Score=446, Evalue=1e-125,
Organism=Escherichia coli, GI145693187, Length=691, Percent_Identity=47.0332850940666, Blast_Score=608, Evalue=1e-175,
Organism=Caenorhabditis elegans, GI115534063, Length=676, Percent_Identity=33.4319526627219, Blast_Score=347, Evalue=1e-95,
Organism=Drosophila melanogaster, GI281362905, Length=686, Percent_Identity=37.1720116618076, Blast_Score=419, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24651641, Length=686, Percent_Identity=37.1720116618076, Blast_Score=419, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24651643, Length=686, Percent_Identity=37.1720116618076, Blast_Score=419, Evalue=1e-117,
Organism=Drosophila melanogaster, GI161079377, Length=632, Percent_Identity=37.1835443037975, Blast_Score=392, Evalue=1e-109,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 77894; Mature: 77762

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEYTRSISFYGREIDINIGLMAPQAGCGVWLTSGETSILVTATRQPGRPGVDFMPLLVD
CCCHHCCHHEECCEEEEEEEEECCCCCCCEEEECCCCEEEEEECCCCCCCCCCHHHHHHH
YEERLYAAGRIPGGYLRREGRPPERATLISRLIDRPIRPLFPEWLRDDVQVVATTLSVDD
HHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEEEECCC
TVPPDVLCILGASLAIHGARIPFNGPVAAVRVGLVKDEFILNPTYAEIEAGDLDLVVAGC
CCCHHHHHHHCCHHHEECCCCCCCCCHHHHEEEEECCCEEECCCHHEEECCCEEEEEEEC
ADGVIMVEAGANQLPEKDVVEAIEFGFEAIQELLKAQQQVLADLNITPVELPPPPKNEEL
CCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHH
IAFVEEQAQEGIRSILRQFLDKTSREQQLEELKAKLEAQIQERPEGDPLRLYLLENPKEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHH
DNQFKALLKKLMRQQILQEGVRVDGRKLDEVRPVSCRVGLIPRVHGSALFNRGLTQVLSI
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHH
TTLGTPGDAQELDDLHPVDEKRYMHHYNFPGFSVGETRPSRSPGRREIGHGALAERALVP
EECCCCCCHHHHHCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHCCCCC
VLPKEEEFPYVVRVVSEVLSSNGSTSMGSVCGSTLSLMDAGVPIKAPVSGVAMGLIKEGD
CCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCC
EVRILTDIQGIEDFLGDMDFKVAGTRAGITALQMDMKITGITVDVVEQAIRQAKAGREFI
CEEEEEHHHHHHHHHCCCCEEEECCCCCCEEEEEEEEEECEEHHHHHHHHHHHHCCHHHH
LDKMLETIAAPRPQLAKTAPRLLTFKVDPEDIGKIIGPGGKTVRGITEATGAKVDISDDG
HHHHHHHHCCCCCHHHHCCCCEEEEEECHHHHHHHCCCCCCCCCCHHHCCCCEEEECCCC
TITVSSSVGGQAEAARAMIENLVRRVEEGQVYLGKVTRIIPIGAFVEFLPGKEGMIHISQ
CEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEHHHHHEEHHHHHHHCCCCCCCEEHHH
LAEYRVGRVEDEVAVEDEVVVKVRSIDHKGRINLTRLGISPEEAARVRNHHH
HHHHHCCCCCCHHCCCCCEEEEEECCCCCCCEEEEEECCCHHHHHHHHCCCC
>Mature Secondary Structure 
AEYTRSISFYGREIDINIGLMAPQAGCGVWLTSGETSILVTATRQPGRPGVDFMPLLVD
CCHHCCHHEECCEEEEEEEEECCCCCCCEEEECCCCEEEEEECCCCCCCCCCHHHHHHH
YEERLYAAGRIPGGYLRREGRPPERATLISRLIDRPIRPLFPEWLRDDVQVVATTLSVDD
HHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEEEECCC
TVPPDVLCILGASLAIHGARIPFNGPVAAVRVGLVKDEFILNPTYAEIEAGDLDLVVAGC
CCCHHHHHHHCCHHHEECCCCCCCCCHHHHEEEEECCCEEECCCHHEEECCCEEEEEEEC
ADGVIMVEAGANQLPEKDVVEAIEFGFEAIQELLKAQQQVLADLNITPVELPPPPKNEEL
CCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHH
IAFVEEQAQEGIRSILRQFLDKTSREQQLEELKAKLEAQIQERPEGDPLRLYLLENPKEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHH
DNQFKALLKKLMRQQILQEGVRVDGRKLDEVRPVSCRVGLIPRVHGSALFNRGLTQVLSI
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHH
TTLGTPGDAQELDDLHPVDEKRYMHHYNFPGFSVGETRPSRSPGRREIGHGALAERALVP
EECCCCCCHHHHHCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHCCCCC
VLPKEEEFPYVVRVVSEVLSSNGSTSMGSVCGSTLSLMDAGVPIKAPVSGVAMGLIKEGD
CCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCC
EVRILTDIQGIEDFLGDMDFKVAGTRAGITALQMDMKITGITVDVVEQAIRQAKAGREFI
CEEEEEHHHHHHHHHCCCCEEEECCCCCCEEEEEEEEEECEEHHHHHHHHHHHHCCHHHH
LDKMLETIAAPRPQLAKTAPRLLTFKVDPEDIGKIIGPGGKTVRGITEATGAKVDISDDG
HHHHHHHHCCCCCHHHHCCCCEEEEEECHHHHHHHCCCCCCCCCCHHHCCCCEEEECCCC
TITVSSSVGGQAEAARAMIENLVRRVEEGQVYLGKVTRIIPIGAFVEFLPGKEGMIHISQ
CEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEHHHHHEEHHHHHHHCCCCCCCEEHHH
LAEYRVGRVEDEVAVEDEVVVKVRSIDHKGRINLTRLGISPEEAARVRNHHH
HHHHHCCCCCCHHCCCCCEEEEEECCCCCCCEEEEEECCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA