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The map label for this gene is leuA [H]

Identifier: 86608910

GI number: 86608910

Start: 1511096

End: 1512736

Strand: Reverse

Name: leuA [H]

Synonym: CYB_1442

Alternate gene names: 86608910

Gene position: 1512736-1511096 (Counterclockwise)

Preceding gene: 86608911

Following gene: 86608905

Centisome position: 49.65

GC content: 59.9

Gene sequence:

>1641_bases
ATGGATGCTGCCGCGGTCGCCGGGTTTGATGGCCAAGCTTTGGATCATGTACTCATTTTCGACACCACCCTGCGAGACGG
GGAGCAGTGCCCCGGCGCCACCCTCAACACCGAAGAAAAGCTGGCCATTGCCCGGCAGTTGGCCCGGCTGGGCGTCGATA
TCATCGAAGCGGGATTTGCCTATGCCAGCCCTGGTGACTTTGAGGCCGTGCAGAAGGTGGCCGAGCAGGTGGGAACCCCC
GATGGCCCTGTGATTTGCAGCCTGGCCCGCGCCCTCAAGCCGGACATTGAGGCCGCCGCCGCCGCCCTAAAGCCTGCTGC
CAAGCCCCGCATCCACACCTTCATCTCCACCTCCGACATTCACCTGAAGTATCAGTTGCGCAAAACCCGCGCTGAGGTGC
TGCAAATTGCCGAAGAGATGGTGGCCTACGCCAAGTCCTTCGTGGAGGATGTGGAGTTTTCCATGATGGATGCCACCCGC
TCTGACCCAGAGTTCCTATACCAGGTCGTCGAGGCGGCCATTCGGGCGGGGGCCAAAACCATCAACATCCCCGATACCGT
CGGCTACGTCATGCCGGAGGAGTTCGGCCAACTGATCAAGGGCATCCGCGAAAATGTGCCCAACATCAAGCAAGCGATCC
TTTCTGTGCACGGCCACGACGATCTGGGCGTCTCGGTGGCCAACTTCTTGGAAGCGATTAAGCAGGGCGCCCGTCAGGTG
GAAGTGACGGTCAACGGCATTGGTGAGCGGGCCGGCAACACAGCCCTGGAAGAGTTGGTGATGGCCTTGCATGTGCGGCG
CAGCTACTTCAACCCCTACTTCGGCCTGCCGGTGGACTCCGAGAAGCCCCTCACCCACATCAAAACTCAGGAGATCTATC
GCACCTCGCGCCTCGTCTCCAACCTCACTGGCATGTTGGTGCAGCCCAACAAGGCTATCGTCGGCTCCAACGCCTTTGCC
CACGAATCTGGCATCCACCAGGACGGCATGTTGAAGAACCGCCAGACCTATGAGATCATGGATGCACAGACCATCGGCCT
GCAATCGAGCACCTTGGTGTTGGGCAAGCACTCGGGCCGCAACGCCTTTCGCAACCGCCTCAAAGAACTGGGGTTCGAGC
TGTCGGAGCAGGATCTGAACAAGGCCTTCTTGCGCTTCAAAGAGCTGGCTGACAAGAAAAAAGAAATCTCCGACCTGGAT
CTGGAAGCGATTGTTAACGACGAAACTCGCCCGGTGCCCGAACTCTACCGCCTGGAGCATGTCCAAGTGGTGTGTGGCAA
TCGAGAGGCTCCCACTGCCACGGTGCGCCTGGTAACCCCGGAAGGGGAAGAGCGGGTGGACGCTGCCGTGGGCACAGGCC
CGGTGGATGCGGTGTACAAGGCCATCAACCGCATTGTCAATATCCCCAACAAGTTAATCGAGTTTTCTGTCCAATCGGTT
ACTGCCGGCATCGACGCCATTGGGGAAGTCACCATCCGCCTGCGCCACGAAGACCGCATCTTTTCCGGCCATGCCGCCAA
CACCGACATCATCGTCGCTTCGGCCCAAGCCTACATGAATGCCCTCAACAAGCTCTACTCTGCGCTGCACGCGGCACGCT
CGACGGCTGACCAGCGCGAGGCTGCTGCCCAGAAGATCTGA

Upstream 100 bases:

>100_bases
TCTCAACAGAGCCCACCCAAATCTTCCCCAAACAAGGCTAGAACCCCTGCCAGTTAATGTCAAAATAGTAAGGCAATGTT
TCGTTATCTCAGTAACCGTT

Downstream 100 bases:

>100_bases
GGGAAGGGTTAGGGAATCCCAGTCGTTTCCAACAAAGCTGGGATCCTTCGCTGCTGACCCTCACCCCCAGCCCCTCTCCT
CTTGGGGAAGAAGGGAGCCC

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 546; Mature: 546

Protein sequence:

>546_residues
MDAAAVAGFDGQALDHVLIFDTTLRDGEQCPGATLNTEEKLAIARQLARLGVDIIEAGFAYASPGDFEAVQKVAEQVGTP
DGPVICSLARALKPDIEAAAAALKPAAKPRIHTFISTSDIHLKYQLRKTRAEVLQIAEEMVAYAKSFVEDVEFSMMDATR
SDPEFLYQVVEAAIRAGAKTINIPDTVGYVMPEEFGQLIKGIRENVPNIKQAILSVHGHDDLGVSVANFLEAIKQGARQV
EVTVNGIGERAGNTALEELVMALHVRRSYFNPYFGLPVDSEKPLTHIKTQEIYRTSRLVSNLTGMLVQPNKAIVGSNAFA
HESGIHQDGMLKNRQTYEIMDAQTIGLQSSTLVLGKHSGRNAFRNRLKELGFELSEQDLNKAFLRFKELADKKKEISDLD
LEAIVNDETRPVPELYRLEHVQVVCGNREAPTATVRLVTPEGEERVDAAVGTGPVDAVYKAINRIVNIPNKLIEFSVQSV
TAGIDAIGEVTIRLRHEDRIFSGHAANTDIIVASAQAYMNALNKLYSALHAARSTADQREAAAQKI

Sequences:

>Translated_546_residues
MDAAAVAGFDGQALDHVLIFDTTLRDGEQCPGATLNTEEKLAIARQLARLGVDIIEAGFAYASPGDFEAVQKVAEQVGTP
DGPVICSLARALKPDIEAAAAALKPAAKPRIHTFISTSDIHLKYQLRKTRAEVLQIAEEMVAYAKSFVEDVEFSMMDATR
SDPEFLYQVVEAAIRAGAKTINIPDTVGYVMPEEFGQLIKGIRENVPNIKQAILSVHGHDDLGVSVANFLEAIKQGARQV
EVTVNGIGERAGNTALEELVMALHVRRSYFNPYFGLPVDSEKPLTHIKTQEIYRTSRLVSNLTGMLVQPNKAIVGSNAFA
HESGIHQDGMLKNRQTYEIMDAQTIGLQSSTLVLGKHSGRNAFRNRLKELGFELSEQDLNKAFLRFKELADKKKEISDLD
LEAIVNDETRPVPELYRLEHVQVVCGNREAPTATVRLVTPEGEERVDAAVGTGPVDAVYKAINRIVNIPNKLIEFSVQSV
TAGIDAIGEVTIRLRHEDRIFSGHAANTDIIVASAQAYMNALNKLYSALHAARSTADQREAAAQKI
>Mature_546_residues
MDAAAVAGFDGQALDHVLIFDTTLRDGEQCPGATLNTEEKLAIARQLARLGVDIIEAGFAYASPGDFEAVQKVAEQVGTP
DGPVICSLARALKPDIEAAAAALKPAAKPRIHTFISTSDIHLKYQLRKTRAEVLQIAEEMVAYAKSFVEDVEFSMMDATR
SDPEFLYQVVEAAIRAGAKTINIPDTVGYVMPEEFGQLIKGIRENVPNIKQAILSVHGHDDLGVSVANFLEAIKQGARQV
EVTVNGIGERAGNTALEELVMALHVRRSYFNPYFGLPVDSEKPLTHIKTQEIYRTSRLVSNLTGMLVQPNKAIVGSNAFA
HESGIHQDGMLKNRQTYEIMDAQTIGLQSSTLVLGKHSGRNAFRNRLKELGFELSEQDLNKAFLRFKELADKKKEISDLD
LEAIVNDETRPVPELYRLEHVQVVCGNREAPTATVRLVTPEGEERVDAAVGTGPVDAVYKAINRIVNIPNKLIEFSVQSV
TAGIDAIGEVTIRLRHEDRIFSGHAANTDIIVASAQAYMNALNKLYSALHAARSTADQREAAAQKI

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=514, Percent_Identity=49.4163424124514, Blast_Score=469, Evalue=1e-133,
Organism=Saccharomyces cerevisiae, GI6320071, Length=401, Percent_Identity=28.428927680798, Blast_Score=147, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6320019, Length=390, Percent_Identity=30.2564102564103, Blast_Score=147, Evalue=6e-36,
Organism=Saccharomyces cerevisiae, GI6324225, Length=505, Percent_Identity=24.1584158415842, Blast_Score=93, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6324682, Length=514, Percent_Identity=23.3463035019455, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24582381, Length=271, Percent_Identity=27.3062730627306, Blast_Score=65, Evalue=8e-11,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 59673; Mature: 59673

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAAAVAGFDGQALDHVLIFDTTLRDGEQCPGATLNTEEKLAIARQLARLGVDIIEAGFA
CCCCCCCCCCCHHHCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHCCC
YASPGDFEAVQKVAEQVGTPDGPVICSLARALKPDIEAAAAALKPAAKPRIHTFISTSDI
CCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEEEECCCE
HLKYQLRKTRAEVLQIAEEMVAYAKSFVEDVEFSMMDATRSDPEFLYQVVEAAIRAGAKT
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCE
INIPDTVGYVMPEEFGQLIKGIRENVPNIKQAILSVHGHDDLGVSVANFLEAIKQGARQV
ECCCCHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCEEE
EVTVNGIGERAGNTALEELVMALHVRRSYFNPYFGLPVDSEKPLTHIKTQEIYRTSRLVS
EEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
NLTGMLVQPNKAIVGSNAFAHESGIHQDGMLKNRQTYEIMDAQTIGLQSSTLVLGKHSGR
HHHCEEECCCCEEECCCCHHHCCCCCCCCCCCCCCCEEECCHHHCCCCCCEEEEECCCCC
NAFRNRLKELGFELSEQDLNKAFLRFKELADKKKEISDLDLEAIVNDETRPVPELYRLEH
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHHHHHH
VQVVCGNREAPTATVRLVTPEGEERVDAAVGTGPVDAVYKAINRIVNIPNKLIEFSVQSV
EEEEECCCCCCCEEEEEECCCCHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHH
TAGIDAIGEVTIRLRHEDRIFSGHAANTDIIVASAQAYMNALNKLYSALHAARSTADQRE
HHHHHHHCEEEEEEEECCCEECCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHH
AAAQKI
HHHCCC
>Mature Secondary Structure
MDAAAVAGFDGQALDHVLIFDTTLRDGEQCPGATLNTEEKLAIARQLARLGVDIIEAGFA
CCCCCCCCCCCHHHCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHCCC
YASPGDFEAVQKVAEQVGTPDGPVICSLARALKPDIEAAAAALKPAAKPRIHTFISTSDI
CCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEEEECCCE
HLKYQLRKTRAEVLQIAEEMVAYAKSFVEDVEFSMMDATRSDPEFLYQVVEAAIRAGAKT
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCE
INIPDTVGYVMPEEFGQLIKGIRENVPNIKQAILSVHGHDDLGVSVANFLEAIKQGARQV
ECCCCHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCEEE
EVTVNGIGERAGNTALEELVMALHVRRSYFNPYFGLPVDSEKPLTHIKTQEIYRTSRLVS
EEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
NLTGMLVQPNKAIVGSNAFAHESGIHQDGMLKNRQTYEIMDAQTIGLQSSTLVLGKHSGR
HHHCEEECCCCEEECCCCHHHCCCCCCCCCCCCCCCEEECCHHHCCCCCCEEEEECCCCC
NAFRNRLKELGFELSEQDLNKAFLRFKELADKKKEISDLDLEAIVNDETRPVPELYRLEH
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHHHHHH
VQVVCGNREAPTATVRLVTPEGEERVDAAVGTGPVDAVYKAINRIVNIPNKLIEFSVQSV
EEEEECCCCCCCEEEEEECCCCHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHH
TAGIDAIGEVTIRLRHEDRIFSGHAANTDIIVASAQAYMNALNKLYSALHAARSTADQRE
HHHHHHHCEEEEEEEECCCEECCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHH
AAAQKI
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA