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The map label for this gene is dapF [H]

Identifier: 86608845

GI number: 86608845

Start: 1441288

End: 1442145

Strand: Reverse

Name: dapF [H]

Synonym: CYB_1371

Alternate gene names: 86608845

Gene position: 1442145-1441288 (Counterclockwise)

Preceding gene: 86608846

Following gene: 86608842

Centisome position: 47.33

GC content: 61.77

Gene sequence:

>858_bases
TTGACCCCCCGTCTCTCCTTTCACAAGTACCAGGGGTTGGGGAACGATTTTATCTTGGTGGACAACCGCTGGCAGTCTCA
ACCCTGCTTGAGCCCGGAAGAGGCCGTCGCCCTTTGCAATCGCCGCTTTGGGGTAGGAGCCGATGGGGTTATCTTCCTGC
TGCCGGGGCAGGAGGGGGCAGATTTCAGCATGCGCCTCTTCAACAGCGACGGTTCCGAGGCGGAGATGTGCGGCAATGGG
ATCCGCTGTTTGGCCCGCTTTCTGCAGGACTTGGGGATCCCAGGTCGGGATGGCGCCTACCAGATCCACACCTTGGCGGG
GCGAATCGTGCCGCAGGTGCGGGCAGATGGGCTGGTCACAGTGGACATGGGGATCCCTCGCCTTTTGGCCAAGCAGATCC
CCACCACCCTGGTCGAACCGGATGAGAAGGTCATTCGGCAGCCGCTTGCAGTGGCCGGGCGAGAGTGGCGGGTGACGGCG
GTGAGCATGGGCAATCCTCATTGTGTGGTCTTCCTGGAAGAAGAGGGATCCCTGGAGGAGCTGGACTTGGCAGCTGTGGG
CCCCCTGTTTGAGCATCATCCGGCCTTTCCTGAGCGCACCAACACCGAATTTGTGCAGGTGCTCAGCCCGACTCGTCTGC
GCCTACGGGTTTGGGAGCGGGGAGCAGGGGCAACGCTGGCTTGTGGGACGGGTGCCTGTGCTGTTTTGGTGGCTGCTGTT
TTGGAGGAGCGCGCAGAATCGCAAGCTACAGTAGAACTGCCGGGAGGAAATCTGGAAATTCGCTGGGATCCGAGCACACA
GCACGTGTGGATGACCGGCCCTGCCCTACACGTCTTCAGCGGCACCACTGCCGGTTGA

Upstream 100 bases:

>100_bases
ACACGGCCTTTGGCCCCCCTTCCAAGGACTTGCAGGATCCCTACGAGATGCACGAGGATTAAGGCCTGTCGTTGGGATAT
CTGGGTAAATGTCTGCTCGG

Downstream 100 bases:

>100_bases
GAACTAGGCTCCCAGACCCAACAGGAGGATGGCCAGGAGGCCGAGGATCCCATAGGCAACGCTCCAGCCCTGCCGCCAGA
TCAGCGCCACTGCCAGGCCC

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase [H]

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MTPRLSFHKYQGLGNDFILVDNRWQSQPCLSPEEAVALCNRRFGVGADGVIFLLPGQEGADFSMRLFNSDGSEAEMCGNG
IRCLARFLQDLGIPGRDGAYQIHTLAGRIVPQVRADGLVTVDMGIPRLLAKQIPTTLVEPDEKVIRQPLAVAGREWRVTA
VSMGNPHCVVFLEEEGSLEELDLAAVGPLFEHHPAFPERTNTEFVQVLSPTRLRLRVWERGAGATLACGTGACAVLVAAV
LEERAESQATVELPGGNLEIRWDPSTQHVWMTGPALHVFSGTTAG

Sequences:

>Translated_285_residues
MTPRLSFHKYQGLGNDFILVDNRWQSQPCLSPEEAVALCNRRFGVGADGVIFLLPGQEGADFSMRLFNSDGSEAEMCGNG
IRCLARFLQDLGIPGRDGAYQIHTLAGRIVPQVRADGLVTVDMGIPRLLAKQIPTTLVEPDEKVIRQPLAVAGREWRVTA
VSMGNPHCVVFLEEEGSLEELDLAAVGPLFEHHPAFPERTNTEFVQVLSPTRLRLRVWERGAGATLACGTGACAVLVAAV
LEERAESQATVELPGGNLEIRWDPSTQHVWMTGPALHVFSGTTAG
>Mature_284_residues
TPRLSFHKYQGLGNDFILVDNRWQSQPCLSPEEAVALCNRRFGVGADGVIFLLPGQEGADFSMRLFNSDGSEAEMCGNGI
RCLARFLQDLGIPGRDGAYQIHTLAGRIVPQVRADGLVTVDMGIPRLLAKQIPTTLVEPDEKVIRQPLAVAGREWRVTAV
SMGNPHCVVFLEEEGSLEELDLAAVGPLFEHHPAFPERTNTEFVQVLSPTRLRLRVWERGAGATLACGTGACAVLVAAVL
EERAESQATVELPGGNLEIRWDPSTQHVWMTGPALHVFSGTTAG

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87082334, Length=281, Percent_Identity=40.5693950177936, Blast_Score=197, Evalue=5e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001653
- InterPro:   IPR018510 [H]

Pfam domain/function: PF01678 DAP_epimerase [H]

EC number: =5.1.1.7 [H]

Molecular weight: Translated: 30950; Mature: 30819

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPRLSFHKYQGLGNDFILVDNRWQSQPCLSPEEAVALCNRRFGVGADGVIFLLPGQEGA
CCCCCCCHHHCCCCCCEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCC
DFSMRLFNSDGSEAEMCGNGIRCLARFLQDLGIPGRDGAYQIHTLAGRIVPQVRADGLVT
CEEEEEECCCCCHHHHHCCHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHCCCCCEEE
VDMGIPRLLAKQIPTTLVEPDEKVIRQPLAVAGREWRVTAVSMGNPHCVVFLEEEGSLEE
ECCCCHHHHHHHCCHHHCCCCHHHHHCHHHHCCCCEEEEEEECCCCEEEEEECCCCCHHH
LDLAAVGPLFEHHPAFPERTNTEFVQVLSPTRLRLRVWERGAGATLACGTGACAVLVAAV
CCHHHHCCHHHCCCCCCCCCCCCEEEECCCCEEEEEEEECCCCCEEEECCCHHHHHHHHH
LEERAESQATVELPGGNLEIRWDPSTQHVWMTGPALHVFSGTTAG
HHHHCCCCEEEEECCCEEEEEECCCCCEEEEECCEEEEECCCCCC
>Mature Secondary Structure 
TPRLSFHKYQGLGNDFILVDNRWQSQPCLSPEEAVALCNRRFGVGADGVIFLLPGQEGA
CCCCCCHHHCCCCCCEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCC
DFSMRLFNSDGSEAEMCGNGIRCLARFLQDLGIPGRDGAYQIHTLAGRIVPQVRADGLVT
CEEEEEECCCCCHHHHHCCHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHCCCCCEEE
VDMGIPRLLAKQIPTTLVEPDEKVIRQPLAVAGREWRVTAVSMGNPHCVVFLEEEGSLEE
ECCCCHHHHHHHCCHHHCCCCHHHHHCHHHHCCCCEEEEEEECCCCEEEEEECCCCCHHH
LDLAAVGPLFEHHPAFPERTNTEFVQVLSPTRLRLRVWERGAGATLACGTGACAVLVAAV
CCHHHHCCHHHCCCCCCCCCCCCEEEECCCCEEEEEEEECCCCCEEEECCCHHHHHHHHH
LEERAESQATVELPGGNLEIRWDPSTQHVWMTGPALHVFSGTTAG
HHHHCCCCEEEEECCCEEEEEECCCCCEEEEECCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA