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The map label for this gene is hisZ

Identifier: 86608577

GI number: 86608577

Start: 1133482

End: 1134786

Strand: Direct

Name: hisZ

Synonym: CYB_1100

Alternate gene names: 86608577

Gene position: 1133482-1134786 (Clockwise)

Preceding gene: 86608576

Following gene: 86608579

Centisome position: 37.2

GC content: 65.21

Gene sequence:

>1305_bases
GTGTACGGTCGCGGTAGCGGTGCGGAGCACTCTCGCGGTAGCGGTGCGGAGCACTTTTGGGATCCCCGCCCTGAGGCCAG
TTCCACCGTCTCCAGCAGTCTGCGCCCCCCCAGCGGTGCCCGGGATCTCCTGCCGCGGGAAGTACAACGGCGGGAGAAGC
TGGAGGCTCAGCTGACCCGGGTTTTCCGGCGCCATGGCTACCAGCGCATCATCACTCCCACCCTAGAGCGGCTGGAAACG
CTGCTGGCGGGGGGATCCATCCGCGCTGAGTCGGTGCTGCAACTGCGGGATGGCGAAGGGACGATGCTGGGGCTGCGCCC
CGAGTTCACTGCTTCCATTGTGCGGGCGGCAGCCACCCGTCTAGCCGGTGGGCCTTTGCCGTTGCGGCTCTACTACCACG
GCAGCGTTTTTCGCAACAGCCGGCGGGAGGAGGGATCCTACAGTAGCCAGGAGTTTTTTCAGTCCGGAGTGGAGCTGATC
GGGGCAGGGGGATGGCTGGCAGATGCCGAGATTCTGCTGCTGCTGGCCGATTGCATTCGCTCGGTCGGCATCTCCTCTTG
TGAGTTCTCTTGGACGCTGTTGCTGGGAGATGTCAGCCTCACCGAGAGTTTGCTCAGCGCCGTTGCCCCCACGGCTCAAG
CGGCAGTACGCCGTGCCATTGCCCAGCTCGACTACGTTTACCTGGAATCGGCCCCTCTGCCGGAAGCCGCCCGCCAGATT
GGCTTGCAGATTCTGGGGCTGCGGGGCCAGCCCGGCTCAGTTCTTTCTGACCTGGCTCAGTTGCCTGTCCCGCCTGAGCG
GCTGCGGGATCTGCGGCAGCTCTGCCAGGTGCTGGAGGAACATGGGGTGCGGGTGGTGCTGGATCTGAGCTTGTTGCAAA
CCCTGGCCTACTACACCGGCATCGTTTTTCAGGCGGTGGCGAGCGGGGAGATCATTGCTCTGGGGGGACGCTACGACCGG
CTGTACGCCCTCTACAGCCCTCAGCAGGTCGAACAGCCGGGGATTGGCTTTACCCTTCTGCCGGATACGCTGCTGCGGCT
CTTGCCCCCCGATCCCCAAACCGAGGAGATGGGCTGCAAACGGTTGGTGGTGCCCCTGGTGCCGGCAGGGATCCCGGCGG
CGCTGGCCTTGGCCGCCCGCTGGCGAGAAGAGTGCACCGCACCGCTGACGCGAACGGAGGCGGTGGAGCTGGAATTGCTG
GATCGGGCCCCTGAAGAGATCGAAGCCTATGCCCGCCAATGTCGGATCCCGGAGATCGCCTGGGTACAAGCCGACGGCAG
CTACCACATCACCCACCCGGGCTAA

Upstream 100 bases:

>100_bases
TCCACGGATCCCATCTGGGAGTGGCCTGAGTTTCCCCGGGATCCCAGCTGACCTCCAACATCCGGTATGATGCAGCAGGA
TCCTCTGCTGGAGCAGCCCT

Downstream 100 bases:

>100_bases
TGCTGGCAAGGTTGACCTCCCAACAGAGACGGGGCTGCACCAGTTGCAACAGGTGTTGGCGAATGCGTTCCAACTGCCGG
ATGGCGCTCTGCACCCCTGG

Product: ATP phosphoribosyltransferase regulatory subunit

Products: AMP; diphosphate +L-histidyl-tRNAHis [C]

Alternate protein names: NA

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MYGRGSGAEHSRGSGAEHFWDPRPEASSTVSSSLRPPSGARDLLPREVQRREKLEAQLTRVFRRHGYQRIITPTLERLET
LLAGGSIRAESVLQLRDGEGTMLGLRPEFTASIVRAAATRLAGGPLPLRLYYHGSVFRNSRREEGSYSSQEFFQSGVELI
GAGGWLADAEILLLLADCIRSVGISSCEFSWTLLLGDVSLTESLLSAVAPTAQAAVRRAIAQLDYVYLESAPLPEAARQI
GLQILGLRGQPGSVLSDLAQLPVPPERLRDLRQLCQVLEEHGVRVVLDLSLLQTLAYYTGIVFQAVASGEIIALGGRYDR
LYALYSPQQVEQPGIGFTLLPDTLLRLLPPDPQTEEMGCKRLVVPLVPAGIPAALALAARWREECTAPLTRTEAVELELL
DRAPEEIEAYARQCRIPEIAWVQADGSYHITHPG

Sequences:

>Translated_434_residues
MYGRGSGAEHSRGSGAEHFWDPRPEASSTVSSSLRPPSGARDLLPREVQRREKLEAQLTRVFRRHGYQRIITPTLERLET
LLAGGSIRAESVLQLRDGEGTMLGLRPEFTASIVRAAATRLAGGPLPLRLYYHGSVFRNSRREEGSYSSQEFFQSGVELI
GAGGWLADAEILLLLADCIRSVGISSCEFSWTLLLGDVSLTESLLSAVAPTAQAAVRRAIAQLDYVYLESAPLPEAARQI
GLQILGLRGQPGSVLSDLAQLPVPPERLRDLRQLCQVLEEHGVRVVLDLSLLQTLAYYTGIVFQAVASGEIIALGGRYDR
LYALYSPQQVEQPGIGFTLLPDTLLRLLPPDPQTEEMGCKRLVVPLVPAGIPAALALAARWREECTAPLTRTEAVELELL
DRAPEEIEAYARQCRIPEIAWVQADGSYHITHPG
>Mature_434_residues
MYGRGSGAEHSRGSGAEHFWDPRPEASSTVSSSLRPPSGARDLLPREVQRREKLEAQLTRVFRRHGYQRIITPTLERLET
LLAGGSIRAESVLQLRDGEGTMLGLRPEFTASIVRAAATRLAGGPLPLRLYYHGSVFRNSRREEGSYSSQEFFQSGVELI
GAGGWLADAEILLLLADCIRSVGISSCEFSWTLLLGDVSLTESLLSAVAPTAQAAVRRAIAQLDYVYLESAPLPEAARQI
GLQILGLRGQPGSVLSDLAQLPVPPERLRDLRQLCQVLEEHGVRVVLDLSLLQTLAYYTGIVFQAVASGEIIALGGRYDR
LYALYSPQQVEQPGIGFTLLPDTLLRLLPPDPQTEEMGCKRLVVPLVPAGIPAALALAARWREECTAPLTRTEAVELELL
DRAPEEIEAYARQCRIPEIAWVQADGSYHITHPG

Specific function: Required for the first step of histidine biosynthesis. May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine

COG id: COG3705

COG function: function code E; ATP phosphoribosyltransferase involved in histidine biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. HisZ subfamily

Homologues:

Organism=Homo sapiens, GI6996014, Length=339, Percent_Identity=28.023598820059, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI15029520, Length=338, Percent_Identity=26.0355029585799, Blast_Score=90, Evalue=4e-18,
Organism=Escherichia coli, GI1788861, Length=373, Percent_Identity=27.0777479892761, Blast_Score=86, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6325290, Length=368, Percent_Identity=22.554347826087, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24643061, Length=368, Percent_Identity=23.9130434782609, Blast_Score=89, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HISZ_SYNJB (Q2JMG5)

Other databases:

- EMBL:   CP000240
- RefSeq:   YP_477339.1
- ProteinModelPortal:   Q2JMG5
- SMR:   Q2JMG5
- STRING:   Q2JMG5
- GeneID:   3900530
- GenomeReviews:   CP000240_GR
- KEGG:   cyb:CYB_1100
- TIGR:   CYB_1100
- eggNOG:   COG3705
- HOGENOM:   HBG616575
- OMA:   PEIEVIN
- ProtClustDB:   PRK12292
- BioCyc:   SSP321332:CYB_1100-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00125
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004517
- InterPro:   IPR004516
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00443

Pfam domain/function: PF00587 tRNA-synt_2b

EC number: 6.1.1.21 [C]

Molecular weight: Translated: 47516; Mature: 47516

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYGRGSGAEHSRGSGAEHFWDPRPEASSTVSSSLRPPSGARDLLPREVQRREKLEAQLTR
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
VFRRHGYQRIITPTLERLETLLAGGSIRAESVLQLRDGEGTMLGLRPEFTASIVRAAATR
HHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHEEECCCCCEEECCCHHHHHHHHHHHHH
LAGGPLPLRLYYHGSVFRNSRREEGSYSSQEFFQSGVELIGAGGWLADAEILLLLADCIR
HCCCCCEEEEEECCHHHHCCCCCCCCCCHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHH
SVGISSCEFSWTLLLGDVSLTESLLSAVAPTAQAAVRRAIAQLDYVYLESAPLPEAARQI
HCCCCCCCEEEEEEECCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHEECCCCCHHHHHHC
GLQILGLRGQPGSVLSDLAQLPVPPERLRDLRQLCQVLEEHGVRVVLDLSLLQTLAYYTG
CCEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHH
IVFQAVASGEIIALGGRYDRLYALYSPQQVEQPGIGFTLLPDTLLRLLPPDPQTEEMGCK
HHHHHHCCCCEEEECCCCEEEEEEECCHHCCCCCCCEEECHHHHHHHCCCCCCCHHHCHH
RLVVPLVPAGIPAALALAARWREECTAPLTRTEAVELELLDRAPEEIEAYARQCRIPEIA
HHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCCEE
WVQADGSYHITHPG
EEEECCCEEECCCC
>Mature Secondary Structure
MYGRGSGAEHSRGSGAEHFWDPRPEASSTVSSSLRPPSGARDLLPREVQRREKLEAQLTR
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
VFRRHGYQRIITPTLERLETLLAGGSIRAESVLQLRDGEGTMLGLRPEFTASIVRAAATR
HHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHEEECCCCCEEECCCHHHHHHHHHHHHH
LAGGPLPLRLYYHGSVFRNSRREEGSYSSQEFFQSGVELIGAGGWLADAEILLLLADCIR
HCCCCCEEEEEECCHHHHCCCCCCCCCCHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHH
SVGISSCEFSWTLLLGDVSLTESLLSAVAPTAQAAVRRAIAQLDYVYLESAPLPEAARQI
HCCCCCCCEEEEEEECCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHEECCCCCHHHHHHC
GLQILGLRGQPGSVLSDLAQLPVPPERLRDLRQLCQVLEEHGVRVVLDLSLLQTLAYYTG
CCEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHH
IVFQAVASGEIIALGGRYDRLYALYSPQQVEQPGIGFTLLPDTLLRLLPPDPQTEEMGCK
HHHHHHCCCCEEEECCCCEEEEEEECCHHCCCCCCCEEECHHHHHHHCCCCCCCHHHCHH
RLVVPLVPAGIPAALALAARWREECTAPLTRTEAVELELLDRAPEEIEAYARQCRIPEIA
HHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCCEE
WVQADGSYHITHPG
EEEECCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): 8520 [C]

Specific activity: 121.8

Km value (mM): 0.06 {His}} 0.046 {His}} 0.03 {His}} 0.02 {His}} 0.017 {His}} 0.011 {His}} 0.008 {His}} 0.0014 {tRNAHis}} 0.00056 {tRNAHis}} 0.0016 {U73tRNAHisGUG}} 0.00031 {U73tRNAHisGUG}} 0.89 {ATP}} 0.74 {ATP}} 0.655 {ATP}} 0.5

Substrates: ATP; @HIST01.txt*L-histidine!; tRNAHis [C]

Specific reaction: ATP + @HIST01.txt*L-histidine! + tRNAHis = AMP + diphosphate +L-histidyl-tRNAHis [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 1, 2, 4-Triazole-3-alanine; D-Histidine; Diphosphate; L-Histidinol; Mg2+; N-Acetyl histidine; NEM; p-Chloromercuribenzoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA