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The map label for this gene is tgt [H]

Identifier: 86608004

GI number: 86608004

Start: 524190

End: 525287

Strand: Direct

Name: tgt [H]

Synonym: CYB_0511

Alternate gene names: 86608004

Gene position: 524190-525287 (Clockwise)

Preceding gene: 86608003

Following gene: 86608005

Centisome position: 17.21

GC content: 60.29

Gene sequence:

>1098_bases
TTGGCCGACTTTACCTTTACTTGTGAGGGCACCTGCCCCCATACCCAAGCGCGGGCAGGATACTTCGTCACCCCCCACGG
CGGGATCCCAACCCCTTGCTTTATGCCGGTGGGAACTTTGGCCAACGTGAAAACCCTCACCCCGGCCCAGCTCAAGGAGA
CAGGTGCCCACATCATCCTCAGCAACACCTATCATCTCCACCTGCAACCTGGCGAAAGCATCATTCAAGCGGCAGGGGGG
CTACACCGCTTTATGGGCTGGTCGGGCCCCATCCTTACCGACTCAGGGGGCTTCCAGGTCTTCAGCCTCAGCGCCATTCG
CCAAATCAACGAGGAGGGAGTGACGTTCCGCTCTCCTCTCGATGGCCGCATCATTCACTTCACCCCAGAGCTATCCATCC
AGATTCAAAATGCCTTGGGTGCCGATGTGATCATGGCCTTCGACGAGTGCCCGCCCTACCCTGCCAGCCCTGAGGAAGTG
GAAGCAGCAGTGCGGCGAACCACCCAGTGGCTGAAACGGTGCGTGAAAGCCCATCGCCGCTCAGATCAGGCTTTGTTTGG
CATTGTGCAGGGGGGAGTTTACCCTGAGCTGCGCCGGCGGGCGGTGGAAGAGGTCTGTCGGTTGGATCTGCCGGGCTATG
CCATTGGCGGGGTGAGTGTGGGGGAGCCGGTCGAGTTTATGCACGCCGTTGTGCGGCAAACAGCCCCTCTACTGCCGGCA
GATCGCCCCCGTTATCTCATGGGGGTGGGCACCTATGCCGAGATGCGCGCTGCTGTGGCCGCCGGAGTGGATTTGTTTGA
CTGCGTGATCCCCACCCGCTATGCCCGCCATGGCACGGTGATCAGCCGGGGCAAACGCTGGAACATCAAAAATGCGGAAC
ACCGCCGCAGCTTGGATCCCATCGATCCCGACTGTCCCTGCTACACCTGCCGCCACTTTAGCCGCGCCTACTTGTGCCAC
CTCATCCGCGCCCAGGAGATCCTGGGCTATACCCTGCTTTCTATCCACAACATCACGGAGCTGATGCGCTACACCCAAAA
GATGCGTCAGGCCATCTTGGAAGGCAAGTTTCACGAGAACTGGGATCCCTGGAGCTAA

Upstream 100 bases:

>100_bases
AGGGGATCCCTGTCAAGGCTGGCAAAAGGGATCCCTTGCCGGCGGCTCTATCTGTGCTAAGCTCTGCTGCGCACCAGATG
AAAGCTGATCAACGCTGACC

Downstream 100 bases:

>100_bases
GCCGGTCAGGAGATTTCTCTGGAAATATCCGATCCATCCTAGTTCACTAGATACTCCAATATCCCGTGTATATACTTATA
CTTAAATTATCCCATGTAGC

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MADFTFTCEGTCPHTQARAGYFVTPHGGIPTPCFMPVGTLANVKTLTPAQLKETGAHIILSNTYHLHLQPGESIIQAAGG
LHRFMGWSGPILTDSGGFQVFSLSAIRQINEEGVTFRSPLDGRIIHFTPELSIQIQNALGADVIMAFDECPPYPASPEEV
EAAVRRTTQWLKRCVKAHRRSDQALFGIVQGGVYPELRRRAVEEVCRLDLPGYAIGGVSVGEPVEFMHAVVRQTAPLLPA
DRPRYLMGVGTYAEMRAAVAAGVDLFDCVIPTRYARHGTVISRGKRWNIKNAEHRRSLDPIDPDCPCYTCRHFSRAYLCH
LIRAQEILGYTLLSIHNITELMRYTQKMRQAILEGKFHENWDPWS

Sequences:

>Translated_365_residues
MADFTFTCEGTCPHTQARAGYFVTPHGGIPTPCFMPVGTLANVKTLTPAQLKETGAHIILSNTYHLHLQPGESIIQAAGG
LHRFMGWSGPILTDSGGFQVFSLSAIRQINEEGVTFRSPLDGRIIHFTPELSIQIQNALGADVIMAFDECPPYPASPEEV
EAAVRRTTQWLKRCVKAHRRSDQALFGIVQGGVYPELRRRAVEEVCRLDLPGYAIGGVSVGEPVEFMHAVVRQTAPLLPA
DRPRYLMGVGTYAEMRAAVAAGVDLFDCVIPTRYARHGTVISRGKRWNIKNAEHRRSLDPIDPDCPCYTCRHFSRAYLCH
LIRAQEILGYTLLSIHNITELMRYTQKMRQAILEGKFHENWDPWS
>Mature_364_residues
ADFTFTCEGTCPHTQARAGYFVTPHGGIPTPCFMPVGTLANVKTLTPAQLKETGAHIILSNTYHLHLQPGESIIQAAGGL
HRFMGWSGPILTDSGGFQVFSLSAIRQINEEGVTFRSPLDGRIIHFTPELSIQIQNALGADVIMAFDECPPYPASPEEVE
AAVRRTTQWLKRCVKAHRRSDQALFGIVQGGVYPELRRRAVEEVCRLDLPGYAIGGVSVGEPVEFMHAVVRQTAPLLPAD
RPRYLMGVGTYAEMRAAVAAGVDLFDCVIPTRYARHGTVISRGKRWNIKNAEHRRSLDPIDPDCPCYTCRHFSRAYLCHL
IRAQEILGYTLLSIHNITELMRYTQKMRQAILEGKFHENWDPWS

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=347, Percent_Identity=44.6685878962536, Blast_Score=312, Evalue=4e-85,
Organism=Escherichia coli, GI1786607, Length=345, Percent_Identity=48.4057971014493, Blast_Score=346, Evalue=1e-96,
Organism=Caenorhabditis elegans, GI17544680, Length=337, Percent_Identity=43.026706231454, Blast_Score=278, Evalue=2e-75,
Organism=Drosophila melanogaster, GI19920510, Length=355, Percent_Identity=44.7887323943662, Blast_Score=317, Evalue=6e-87,
Organism=Drosophila melanogaster, GI21355311, Length=222, Percent_Identity=24.7747747747748, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 40711; Mature: 40580

Theoretical pI: Translated: 7.92; Mature: 7.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADFTFTCEGTCPHTQARAGYFVTPHGGIPTPCFMPVGTLANVKTLTPAQLKETGAHIIL
CCCEEEEECCCCCCCCCCCCEEECCCCCCCCCHHCCCCCCCCCCCCCHHHHHHCCCEEEE
SNTYHLHLQPGESIIQAAGGLHRFMGWSGPILTDSGGFQVFSLSAIRQINEEGVTFRSPL
ECEEEEEECCCHHHHHHHHHHHHHHCCCCCEEECCCCCEEEEHHHHHHHHHCCCEEECCC
DGRIIHFTPELSIQIQNALGADVIMAFDECPPYPASPEEVEAAVRRTTQWLKRCVKAHRR
CCEEEEECCCEEEEECCCCCCHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
SDQALFGIVQGGVYPELRRRAVEEVCRLDLPGYAIGGVSVGEPVEFMHAVVRQTAPLLPA
CCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHCCCCCC
DRPRYLMGVGTYAEMRAAVAAGVDLFDCVIPTRYARHGTVISRGKRWNIKNAEHRRSLDP
CCCCEEEECCHHHHHHHHHHHCCHHHHHHHCHHHHHCCCEEECCCCCCCCCHHHHCCCCC
IDPDCPCYTCRHFSRAYLCHLIRAQEILGYTLLSIHNITELMRYTQKMRQAILEGKFHEN
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
WDPWS
CCCCC
>Mature Secondary Structure 
ADFTFTCEGTCPHTQARAGYFVTPHGGIPTPCFMPVGTLANVKTLTPAQLKETGAHIIL
CCEEEEECCCCCCCCCCCCEEECCCCCCCCCHHCCCCCCCCCCCCCHHHHHHCCCEEEE
SNTYHLHLQPGESIIQAAGGLHRFMGWSGPILTDSGGFQVFSLSAIRQINEEGVTFRSPL
ECEEEEEECCCHHHHHHHHHHHHHHCCCCCEEECCCCCEEEEHHHHHHHHHCCCEEECCC
DGRIIHFTPELSIQIQNALGADVIMAFDECPPYPASPEEVEAAVRRTTQWLKRCVKAHRR
CCEEEEECCCEEEEECCCCCCHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
SDQALFGIVQGGVYPELRRRAVEEVCRLDLPGYAIGGVSVGEPVEFMHAVVRQTAPLLPA
CCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHCCCCCC
DRPRYLMGVGTYAEMRAAVAAGVDLFDCVIPTRYARHGTVISRGKRWNIKNAEHRRSLDP
CCCCEEEECCHHHHHHHHHHHCCHHHHHHHCHHHHHCCCEEECCCCCCCCCHHHHCCCCC
IDPDCPCYTCRHFSRAYLCHLIRAQEILGYTLLSIHNITELMRYTQKMRQAILEGKFHEN
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
WDPWS
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]