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The map label for this gene is rumA [C]

Identifier: 86607994

GI number: 86607994

Start: 511015

End: 512409

Strand: Direct

Name: rumA [C]

Synonym: CYB_0500

Alternate gene names: 86607994

Gene position: 511015-512409 (Clockwise)

Preceding gene: 86607993

Following gene: 86607998

Centisome position: 16.77

GC content: 60.14

Gene sequence:

>1395_bases
ATGGAACATCCGATCCCTTCCGGCCTTCCTGATCCCGTTAAACCTGGGGATCTCTTGGCGTTGGAGGTCACGGCCCTGAC
CCCCCAGGGAGATGGCATTGCCCACACGGTAGGACTTGGACAAGGGCGAGTGGTGTTTGTGTCCCAGGCGGTGCCCGGGG
ATCAGGTGGTGGCCCAAATAACCGAGATCAAGCGCAACTACTTGGTCGGGCGAATTCGGTCGCTGATTTCTCCCTCTCCC
CAACGGGTGCGACCAGCCTGCATTGTGGCGGACAAATGTGGTGGCTGCCAATGGCAACATGTCAGCTATGCCCAGCAATT
GGCCACCAAGCAGCAAATCCTCAGGGATGCACTGGAGCGGATTGGCGGTCTGACTCTGCCGCAGCCTGTCCCTATTTTGG
GATCCCAGCAGCCGCTGCGCTATCGCAACAAGGTCACCTACCCCATGGCCAAACCGGAGGGCCAGCCCTTGCGCATGGGC
TACTACCAACGGGGCAGCCACCGCCTGGTGAACCTCAACCAATGCCCCGTGCAAGACGAGCGGCTGGATGTGTTCCTGAG
GGAAATCAAGCGCGATCTTCAAGAGACGGGCTGGAGTGTGTATGACGAAAGTCGTCACCAAGGTCATCTCCGCCACTTGG
GTTTGCGCATCGGTCGCCGCACCCGCGAGGTGCTTCTTACCTTGGTCAGTTGCAGTCGTCGCCTGCCCCATCTGGAAAGC
TGGGCCGAACGCTGGATGCAGCGCTACCCCGACCTGGTGGGGGTCTGTCTCAATCTCAACTCGGCCCGCACCAATGCCAT
CTTCGGCCCGGAGACTGAGGTGATTGCCGGTCGGGGGTCTTTGCGGGAGGAATTTGCCGGTTTTTCCCTCTGGATCGACA
GCACCTCTTTCTTCCAGGTCAACACAGAGCAAGCAGAAGCCTTTTTTACCTGGATAGCCGACCAACTGAACCTGCGGGGA
GAGGAAACCATCCTGGATGCCTACTGTGGCATCGGCACCTTAACCTTGCTGCTGGCGCAACGGGCGAAACGGGCGATGGG
GGTGGAAGCTTTGCCGGAAGCGGTGGCCCAAGCCCGCTACAATGCCCAGTTCAACGGTATCGCCTCAGCCCAATTTTTCC
AGGGCACGGTGGAACAGGTTTTGCCGACCCTGCCCCCTGCCGACATTGTGGTGCTGGATCCGCCGCGGCGCGGCTGCGAC
CGCCAAGTGCTCGAGACCCTAAAGCTGCGACGACCACCACGCATCGTCTACATTAGCTGTCACCCTGCCACTTTGGCGCG
AGACTTGCAGATCCTACTGGCAGAGGGATCCTATCGCCTTACCCAATGGCGGGCGGCTGACTTCTTTCCCCAAACCGGGC
ATGTGGAGGGGGTGGCCTTTTTGGAGCGAGTTTGA

Upstream 100 bases:

>100_bases
TGCTTTTTGCCTTGCTACAGCTTTTTCCGGCAATGCTGACTCCAGCCAACACGACCTGACCTATGGTTATAGTTATTTCG
AATTTCGGCGAAACAGCCTA

Downstream 100 bases:

>100_bases
AGAGTACGCACCGCTGACCAAAAGGCGCGGCAAGCCCCGTCCTTCAGGGCAGGGAAGGATAGCGCCGACCGTAGCCGACG
CCCTTTAAAAACGCAGTGCC

Product: 23S rRNA (uracil-5-)-methyltransferase RumA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MEHPIPSGLPDPVKPGDLLALEVTALTPQGDGIAHTVGLGQGRVVFVSQAVPGDQVVAQITEIKRNYLVGRIRSLISPSP
QRVRPACIVADKCGGCQWQHVSYAQQLATKQQILRDALERIGGLTLPQPVPILGSQQPLRYRNKVTYPMAKPEGQPLRMG
YYQRGSHRLVNLNQCPVQDERLDVFLREIKRDLQETGWSVYDESRHQGHLRHLGLRIGRRTREVLLTLVSCSRRLPHLES
WAERWMQRYPDLVGVCLNLNSARTNAIFGPETEVIAGRGSLREEFAGFSLWIDSTSFFQVNTEQAEAFFTWIADQLNLRG
EETILDAYCGIGTLTLLLAQRAKRAMGVEALPEAVAQARYNAQFNGIASAQFFQGTVEQVLPTLPPADIVVLDPPRRGCD
RQVLETLKLRRPPRIVYISCHPATLARDLQILLAEGSYRLTQWRAADFFPQTGHVEGVAFLERV

Sequences:

>Translated_464_residues
MEHPIPSGLPDPVKPGDLLALEVTALTPQGDGIAHTVGLGQGRVVFVSQAVPGDQVVAQITEIKRNYLVGRIRSLISPSP
QRVRPACIVADKCGGCQWQHVSYAQQLATKQQILRDALERIGGLTLPQPVPILGSQQPLRYRNKVTYPMAKPEGQPLRMG
YYQRGSHRLVNLNQCPVQDERLDVFLREIKRDLQETGWSVYDESRHQGHLRHLGLRIGRRTREVLLTLVSCSRRLPHLES
WAERWMQRYPDLVGVCLNLNSARTNAIFGPETEVIAGRGSLREEFAGFSLWIDSTSFFQVNTEQAEAFFTWIADQLNLRG
EETILDAYCGIGTLTLLLAQRAKRAMGVEALPEAVAQARYNAQFNGIASAQFFQGTVEQVLPTLPPADIVVLDPPRRGCD
RQVLETLKLRRPPRIVYISCHPATLARDLQILLAEGSYRLTQWRAADFFPQTGHVEGVAFLERV
>Mature_464_residues
MEHPIPSGLPDPVKPGDLLALEVTALTPQGDGIAHTVGLGQGRVVFVSQAVPGDQVVAQITEIKRNYLVGRIRSLISPSP
QRVRPACIVADKCGGCQWQHVSYAQQLATKQQILRDALERIGGLTLPQPVPILGSQQPLRYRNKVTYPMAKPEGQPLRMG
YYQRGSHRLVNLNQCPVQDERLDVFLREIKRDLQETGWSVYDESRHQGHLRHLGLRIGRRTREVLLTLVSCSRRLPHLES
WAERWMQRYPDLVGVCLNLNSARTNAIFGPETEVIAGRGSLREEFAGFSLWIDSTSFFQVNTEQAEAFFTWIADQLNLRG
EETILDAYCGIGTLTLLLAQRAKRAMGVEALPEAVAQARYNAQFNGIASAQFFQGTVEQVLPTLPPADIVVLDPPRRGCD
RQVLETLKLRRPPRIVYISCHPATLARDLQILLAEGSYRLTQWRAADFFPQTGHVEGVAFLERV

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=414, Percent_Identity=29.4685990338164, Blast_Score=139, Evalue=6e-33,
Organism=Homo sapiens, GI51173878, Length=414, Percent_Identity=29.4685990338164, Blast_Score=139, Evalue=6e-33,
Organism=Homo sapiens, GI269784680, Length=209, Percent_Identity=29.6650717703349, Blast_Score=100, Evalue=6e-21,
Organism=Homo sapiens, GI269784676, Length=209, Percent_Identity=29.6650717703349, Blast_Score=100, Evalue=6e-21,
Organism=Homo sapiens, GI20149688, Length=209, Percent_Identity=29.6650717703349, Blast_Score=100, Evalue=6e-21,
Organism=Homo sapiens, GI269784678, Length=209, Percent_Identity=29.6650717703349, Blast_Score=99, Evalue=7e-21,
Organism=Escherichia coli, GI1789148, Length=461, Percent_Identity=29.2841648590022, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI1787083, Length=389, Percent_Identity=25.706940874036, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1790403, Length=179, Percent_Identity=29.0502793296089, Blast_Score=74, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6322909, Length=436, Percent_Identity=22.4770642201835, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24666579, Length=417, Percent_Identity=26.8585131894484, Blast_Score=129, Evalue=5e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr [H]

EC number: NA

Molecular weight: Translated: 51957; Mature: 51957

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEHPIPSGLPDPVKPGDLLALEVTALTPQGDGIAHTVGLGQGRVVFVSQAVPGDQVVAQI
CCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCEEEEECCCCCEEEEECCCCHHHHHHHH
TEIKRNYLVGRIRSLISPSPQRVRPACIVADKCGGCQWQHVSYAQQLATKQQILRDALER
HHHHHHHHHHHHHHHHCCCHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IGGLTLPQPVPILGSQQPLRYRNKVTYPMAKPEGQPLRMGYYQRGSHRLVNLNQCPVQDE
HCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCEEECCEECCCCCEEECCCCCCCHH
RLDVFLREIKRDLQETGWSVYDESRHQGHLRHLGLRIGRRTREVLLTLVSCSRRLPHLES
HHHHHHHHHHHHHHHCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
WAERWMQRYPDLVGVCLNLNSARTNAIFGPETEVIAGRGSLREEFAGFSLWIDSTSFFQV
HHHHHHHHCCHHEEEHCCCCCCCCCCEECCCCCEEECCCHHHHHHCCEEEEECCCEEEEE
NTEQAEAFFTWIADQLNLRGEETILDAYCGIGTLTLLLAQRAKRAMGVEALPEAVAQARY
EHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHC
NAQFNGIASAQFFQGTVEQVLPTLPPADIVVLDPPRRGCDRQVLETLKLRRPPRIVYISC
CCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCHHHHHHHHHCCCCEEEEEEE
HPATLARDLQILLAEGSYRLTQWRAADFFPQTGHVEGVAFLERV
CCHHHHHHHHEEEECCCEEEEEEEHHCCCCCCCCCCHHHHHHCC
>Mature Secondary Structure
MEHPIPSGLPDPVKPGDLLALEVTALTPQGDGIAHTVGLGQGRVVFVSQAVPGDQVVAQI
CCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCEEEEECCCCCEEEEECCCCHHHHHHHH
TEIKRNYLVGRIRSLISPSPQRVRPACIVADKCGGCQWQHVSYAQQLATKQQILRDALER
HHHHHHHHHHHHHHHHCCCHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IGGLTLPQPVPILGSQQPLRYRNKVTYPMAKPEGQPLRMGYYQRGSHRLVNLNQCPVQDE
HCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCEEECCEECCCCCEEECCCCCCCHH
RLDVFLREIKRDLQETGWSVYDESRHQGHLRHLGLRIGRRTREVLLTLVSCSRRLPHLES
HHHHHHHHHHHHHHHCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
WAERWMQRYPDLVGVCLNLNSARTNAIFGPETEVIAGRGSLREEFAGFSLWIDSTSFFQV
HHHHHHHHCCHHEEEHCCCCCCCCCCEECCCCCEEECCCHHHHHHCCEEEEECCCEEEEE
NTEQAEAFFTWIADQLNLRGEETILDAYCGIGTLTLLLAQRAKRAMGVEALPEAVAQARY
EHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHC
NAQFNGIASAQFFQGTVEQVLPTLPPADIVVLDPPRRGCDRQVLETLKLRRPPRIVYISC
CCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCHHHHHHHHHCCCCEEEEEEE
HPATLARDLQILLAEGSYRLTQWRAADFFPQTGHVEGVAFLERV
CCHHHHHHHHEEEECCCEEEEEEEHHCCCCCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]