Click here to switch to the map view.

The map label for this gene is cysH [H]

Identifier: 86607934

GI number: 86607934

Start: 448355

End: 449101

Strand: Direct

Name: cysH [H]

Synonym: CYB_0438

Alternate gene names: 86607934

Gene position: 448355-449101 (Clockwise)

Preceding gene: 86607933

Following gene: 86607935

Centisome position: 14.72

GC content: 59.04

Gene sequence:

>747_bases
ATGACAGCAAGCCTTTCTCCTTCTAGCCGCTGGGAGCTGGCGGCTCAAGCGCTGCGGGAGCAGTATCAACCTCAAGTGGC
GTCCCTGAGCGAACGGTTTGAGCCTTTGTCGGCAAGCGACCTGCTCACCTGGGCGGTTGAAGAATTTGGAGATGGGCTGG
TGCTGGCCTGTAGCTTTGGCCCGGAAGACCTAGTGCTGATCGATCTGCTCACGGCGATTAACCCCCGTGTTCGCGCTTTT
TTTCTGGACACCGATTTTCACTTCCCGGAAACCCTACAACTGCGGGAGCAGGTGTTGGCGCGCTACCCCCACTTGCAACT
GCAGATCTTCAAGCCCTTGCTTACCCCGGAAGAGCAGGCAGCTCAATACGGGCCAGAACTGTATCGCTCTAACCCCGACC
TCTGCTGCAACATCCGCAAGGTGGAGCCCTTGAATCGTGCTCTGGCCGGTTGCACCACTTGGATTACAGGGATGCGTCGG
GAACAATCCCCCACCCGCGCCGATATCGGCAAGGTGCAGTGGGACGGCAAGCGCAACCGCCTCAAACTCAACCCGCTGGC
CGACTGGACAAATGGCCAAGTTTGGAAATACATCCTTGACCACGGGATCCCTTACAACCCCCTGCACGATCGCCACTACC
CCAGTATCGGCTGCCTCCACTGCACTGCGCCTGTTGAGCCAGGGGCGGATCCCCGCTCTGGACGCTGGAAGGGCACAAGC
AAAACCGAATGCGGCCTGCATACCTAA

Upstream 100 bases:

>100_bases
CCTCTGCCCGCTGCCGCCTGGTAATCAAAACCCCTAAAGCTTGACCGAATTAGTGTAGATTAAAAAAAGCAAAAATCGTC
GAGTTCGGTGTCTTCCCCCT

Downstream 100 bases:

>100_bases
CCCATGACTGCTTCCTCCATCCTTGAGGTGGCTCAGTTTGAAATTACCCCCGGCTCTGAAGCCGCCTTTGAAGCCGCCAT
CGCTCAAGCTTTCATCTATT

Product: phosophoadenylyl-sulfate reductase

Products: NA

Alternate protein names: 3'-phosphoadenylylsulfate reductase; PAPS reductase, thioredoxin dependent; PAPS sulfotransferase; PAdoPS reductase [H]

Number of amino acids: Translated: 248; Mature: 247

Protein sequence:

>248_residues
MTASLSPSSRWELAAQALREQYQPQVASLSERFEPLSASDLLTWAVEEFGDGLVLACSFGPEDLVLIDLLTAINPRVRAF
FLDTDFHFPETLQLREQVLARYPHLQLQIFKPLLTPEEQAAQYGPELYRSNPDLCCNIRKVEPLNRALAGCTTWITGMRR
EQSPTRADIGKVQWDGKRNRLKLNPLADWTNGQVWKYILDHGIPYNPLHDRHYPSIGCLHCTAPVEPGADPRSGRWKGTS
KTECGLHT

Sequences:

>Translated_248_residues
MTASLSPSSRWELAAQALREQYQPQVASLSERFEPLSASDLLTWAVEEFGDGLVLACSFGPEDLVLIDLLTAINPRVRAF
FLDTDFHFPETLQLREQVLARYPHLQLQIFKPLLTPEEQAAQYGPELYRSNPDLCCNIRKVEPLNRALAGCTTWITGMRR
EQSPTRADIGKVQWDGKRNRLKLNPLADWTNGQVWKYILDHGIPYNPLHDRHYPSIGCLHCTAPVEPGADPRSGRWKGTS
KTECGLHT
>Mature_247_residues
TASLSPSSRWELAAQALREQYQPQVASLSERFEPLSASDLLTWAVEEFGDGLVLACSFGPEDLVLIDLLTAINPRVRAFF
LDTDFHFPETLQLREQVLARYPHLQLQIFKPLLTPEEQAAQYGPELYRSNPDLCCNIRKVEPLNRALAGCTTWITGMRRE
QSPTRADIGKVQWDGKRNRLKLNPLADWTNGQVWKYILDHGIPYNPLHDRHYPSIGCLHCTAPVEPGADPRSGRWKGTSK
TECGLHT

Specific function: Reduction of activated sulfate into sulfite [H]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysH subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789121, Length=226, Percent_Identity=33.6283185840708, Blast_Score=124, Evalue=7e-30,
Organism=Saccharomyces cerevisiae, GI6325425, Length=236, Percent_Identity=35.5932203389831, Blast_Score=137, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004511
- InterPro:   IPR002500
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =1.8.4.8 [H]

Molecular weight: Translated: 28078; Mature: 27947

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASLSPSSRWELAAQALREQYQPQVASLSERFEPLSASDLLTWAVEEFGDGLVLACSFG
CCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEECCC
PEDLVLIDLLTAINPRVRAFFLDTDFHFPETLQLREQVLARYPHLQLQIFKPLLTPEEQA
CCHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEHHHCCCCHHHH
AQYGPELYRSNPDLCCNIRKVEPLNRALAGCTTWITGMRREQSPTRADIGKVQWDGKRNR
HHCCHHHHCCCCCEEEEEHHCCHHHHHHHHHHHHHHHHHCCCCCCHHCCCCEEECCCCCE
LKLNPLADWTNGQVWKYILDHGIPYNPLHDRHYPSIGCLHCTAPVEPGADPRSGRWKGTS
EEECCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCC
KTECGLHT
CCCCCCCC
>Mature Secondary Structure 
TASLSPSSRWELAAQALREQYQPQVASLSERFEPLSASDLLTWAVEEFGDGLVLACSFG
CCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEECCC
PEDLVLIDLLTAINPRVRAFFLDTDFHFPETLQLREQVLARYPHLQLQIFKPLLTPEEQA
CCHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEHHHCCCCHHHH
AQYGPELYRSNPDLCCNIRKVEPLNRALAGCTTWITGMRREQSPTRADIGKVQWDGKRNR
HHCCHHHHCCCCCEEEEEHHCCHHHHHHHHHHHHHHHHHCCCCCCHHCCCCEEECCCCCE
LKLNPLADWTNGQVWKYILDHGIPYNPLHDRHYPSIGCLHCTAPVEPGADPRSGRWKGTS
EEECCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCC
KTECGLHT
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14621292 [H]