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The map label for this gene is ntcB [H]

Identifier: 86607830

GI number: 86607830

Start: 349223

End: 350188

Strand: Direct

Name: ntcB [H]

Synonym: CYB_0332

Alternate gene names: 86607830

Gene position: 349223-350188 (Clockwise)

Preceding gene: 86607826

Following gene: 86607831

Centisome position: 11.46

GC content: 61.59

Gene sequence:

>966_bases
GTGCGGCTGGAGTTGTTGCAAGCCTTTTTGAACGTGGTGGAAACGGGCAGCTTTTTGGCCGCTGCTCGCCTCAGCGGTCT
GACCCAATCGGCTATTAGCCGCCAGATCCAGGCCCTGGAAGAAGCGGTGGGCGCTCCGCTGTTGCATCGCAAAAGCCCGG
TGGCTCTAACCGTAGCTGGGGAACGGCTTTTGCCCCATGCCCGTCGCATCTGTCAAGAATGGCAGCGGGCTCAGGAAAGC
ATTCGAGAATGGCTGCAGGGCCAGCAAACCGAGCTCTGCATTGCTGGGATCCATTCTCTGATCGCCCATCAACTGCCGCC
GGTGCTGCAACAGTTTTGCCGGGAGTATCCAGAGGTGCAGTTGCGGGTTACCGCTCTGGGCAGCGACCGCGCCTTAAAGG
TGCTCAGGGATGGGCTGGTGGATGTGGCCCTGGTGATGGAGAACCCCCTGATGACGCGGGGGGCAGAGCTGGTGGTGGAG
CCACTGTACTCCGAGCCAATTCAAGTATTGATGCCCGCCACCCATCCCCTCAGCCGCTACGAGCGGATCCCCTGGAAGGT
GCTGGCGGAGTATCCCCAGGCTGTGTTCAAGGATGGCTATGCCATGCGCTCCTTGGTGGGATCCCATCTGGCCCGGCAGG
GGCTTCCCCTGAAAGCAGCCCTGGAACTGAACACCTTGGATGCCTTTCGGGGGGTGGTGCGACAGGGGCAGCTCATTGCC
CTCCTGCCGGAGTCGGCCCTGGTGGACTGCTGCGCCGATCCGGATTTGCAGGTGCGACCCACCGAGCCCCCCCTCTTGAG
GCGACAGGTGGTTATGGTTACCACCAGCGATCGCTATCGGATCCCTCCCATTCGCCGGTTTTTGCAGTTGGCCGCCCAGC
TTATCAGGCAGTCTGAGGTTGAGCCTGCTTCCGGTACAGATCCCCATTTCCCTCAGCCCTCTGCCGGGCTGTTGTCAGTT
CTGTAG

Upstream 100 bases:

>100_bases
TCCCCAGTCCAACAAACAACTTGTAACGGTGTTTACAAAAGCCTACCGCTTCCCCAGCTAGGATGCCACCACGGCAGGCT
ACATCATCAAGGGATCCCAG

Downstream 100 bases:

>100_bases
GGATTTTTTGTAGAAATTTCTGTAAGTTGTCAAATACCTGTATCGGTAGTTGCAAGTGTTCCGCCGTTATCTCCCCCTAC
TTGGGTAGCCCAGCGCTCCC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MRLELLQAFLNVVETGSFLAAARLSGLTQSAISRQIQALEEAVGAPLLHRKSPVALTVAGERLLPHARRICQEWQRAQES
IREWLQGQQTELCIAGIHSLIAHQLPPVLQQFCREYPEVQLRVTALGSDRALKVLRDGLVDVALVMENPLMTRGAELVVE
PLYSEPIQVLMPATHPLSRYERIPWKVLAEYPQAVFKDGYAMRSLVGSHLARQGLPLKAALELNTLDAFRGVVRQGQLIA
LLPESALVDCCADPDLQVRPTEPPLLRRQVVMVTTSDRYRIPPIRRFLQLAAQLIRQSEVEPASGTDPHFPQPSAGLLSV
L

Sequences:

>Translated_321_residues
MRLELLQAFLNVVETGSFLAAARLSGLTQSAISRQIQALEEAVGAPLLHRKSPVALTVAGERLLPHARRICQEWQRAQES
IREWLQGQQTELCIAGIHSLIAHQLPPVLQQFCREYPEVQLRVTALGSDRALKVLRDGLVDVALVMENPLMTRGAELVVE
PLYSEPIQVLMPATHPLSRYERIPWKVLAEYPQAVFKDGYAMRSLVGSHLARQGLPLKAALELNTLDAFRGVVRQGQLIA
LLPESALVDCCADPDLQVRPTEPPLLRRQVVMVTTSDRYRIPPIRRFLQLAAQLIRQSEVEPASGTDPHFPQPSAGLLSV
L
>Mature_321_residues
MRLELLQAFLNVVETGSFLAAARLSGLTQSAISRQIQALEEAVGAPLLHRKSPVALTVAGERLLPHARRICQEWQRAQES
IREWLQGQQTELCIAGIHSLIAHQLPPVLQQFCREYPEVQLRVTALGSDRALKVLRDGLVDVALVMENPLMTRGAELVVE
PLYSEPIQVLMPATHPLSRYERIPWKVLAEYPQAVFKDGYAMRSLVGSHLARQGLPLKAALELNTLDAFRGVVRQGQLIA
LLPESALVDCCADPDLQVRPTEPPLLRRQVVMVTTSDRYRIPPIRRFLQLAAQLIRQSEVEPASGTDPHFPQPSAGLLSV
L

Specific function: Seems to regulate utilization of fixed nitrogen by controlling the expression of a certain gene(s) involved in nitrogen metabolism [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI145693105, Length=297, Percent_Identity=26.5993265993266, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI157672245, Length=287, Percent_Identity=26.1324041811847, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1787530, Length=256, Percent_Identity=26.171875, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788748, Length=282, Percent_Identity=28.0141843971631, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787601, Length=180, Percent_Identity=31.1111111111111, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1788887, Length=184, Percent_Identity=33.1521739130435, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1788296, Length=217, Percent_Identity=25.8064516129032, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1787879, Length=252, Percent_Identity=29.7619047619048, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI87081904, Length=252, Percent_Identity=25.3968253968254, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1790399, Length=249, Percent_Identity=24.4979919678715, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1786401, Length=307, Percent_Identity=27.0358306188925, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788481, Length=290, Percent_Identity=23.448275862069, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1790208, Length=145, Percent_Identity=33.1034482758621, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790262, Length=183, Percent_Identity=33.3333333333333, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1789440, Length=177, Percent_Identity=26.5536723163842, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35673; Mature: 35673

Theoretical pI: Translated: 7.92; Mature: 7.92

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLELLQAFLNVVETGSFLAAARLSGLTQSAISRQIQALEEAVGAPLLHRKSPVALTVAG
CHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECC
ERLLPHARRICQEWQRAQESIREWLQGQQTELCIAGIHSLIAHQLPPVLQQFCREYPEVQ
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEE
LRVTALGSDRALKVLRDGLVDVALVMENPLMTRGAELVVEPLYSEPIQVLMPATHPLSRY
EEEEECCCHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHCCHHHHCCCCCCHHHH
ERIPWKVLAEYPQAVFKDGYAMRSLVGSHLARQGLPLKAALELNTLDAFRGVVRQGQLIA
HCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEE
LLPESALVDCCADPDLQVRPTEPPLLRRQVVMVTTSDRYRIPPIRRFLQLAAQLIRQSEV
ECCHHHHHHHCCCCCCEECCCCCHHHHCEEEEEECCCCEECCHHHHHHHHHHHHHHHCCC
EPASGTDPHFPQPSAGLLSVL
CCCCCCCCCCCCCCCHHHHCC
>Mature Secondary Structure
MRLELLQAFLNVVETGSFLAAARLSGLTQSAISRQIQALEEAVGAPLLHRKSPVALTVAG
CHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECC
ERLLPHARRICQEWQRAQESIREWLQGQQTELCIAGIHSLIAHQLPPVLQQFCREYPEVQ
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEE
LRVTALGSDRALKVLRDGLVDVALVMENPLMTRGAELVVEPLYSEPIQVLMPATHPLSRY
EEEEECCCHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHCCHHHHCCCCCCHHHH
ERIPWKVLAEYPQAVFKDGYAMRSLVGSHLARQGLPLKAALELNTLDAFRGVVRQGQLIA
HCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEE
LLPESALVDCCADPDLQVRPTEPPLLRRQVVMVTTSDRYRIPPIRRFLQLAAQLIRQSEV
ECCHHHHHHHCCCCCCEECCCCCHHHHCEEEEEECCCCEECCHHHHHHHHHHHHHHHCCC
EPASGTDPHFPQPSAGLLSVL
CCCCCCCCCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]