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The map label for this gene is leuC [H]

Identifier: 86607826

GI number: 86607826

Start: 344054

End: 345457

Strand: Direct

Name: leuC [H]

Synonym: CYB_0327

Alternate gene names: 86607826

Gene position: 344054-345457 (Clockwise)

Preceding gene: 86607825

Following gene: 86607830

Centisome position: 11.29

GC content: 61.68

Gene sequence:

>1404_bases
ATGAGCGAGAGGACATTATTCGATAAGGTGTGGGATGCCCATACGGTGCGGATTTTGCCCTCTGGGCAGACGCAGTTGTT
CATCGGCCTGCACCTGATCCACGAGGTGACCAGCCCGCAGGCTTTTGCCATGCTGCGGGAGCGTCATCTGCCGGTTCTGT
TTCCCGAGCGGACGGTGGCCACCGTGGATCACATCATTCCCACCGACAACCGGGCACGGCCTTTTGCGGATCCCTTGGCA
GAGGAGATGATCCAGGAATTGGAGCGCAATTGCCGGCAATACGGCATCCGCTTCTACAACAGCGGCTCCGGTCGGCAGGG
GATCGTGCATGTGATTGCCCCGGAGCAAGGGTTAACTCTGCCGGGGATGACCATCGCCTGTGGGGACAGCCACACCTCTA
CCCACGGGGCCTTTGGGGCCATTGCTTTTGGGATCGGCACCAGCCAGGTGCGAGACGTATTGGCCACCCAAACTTTGGCT
TTGTCCAAGCTCAAGGTGCGCCGCATTGAGATCCACGGCAAGTTGGGGCCGGGAGTTTACGCTAAGGATGTAATTCTGCA
CATCATCCGCAAGCTGGGGGTCAAAGGCGGGGTGGGCTACGCCTACGAGTATGGGGGCAGCGCCATTGAAGCCATGAGCA
TGGAAGAGCGCATGACCCTCTGCAACATGTCCATCGAGGGAGGGGCCCGCTGCGGCTACGTCAACCCCGATGCGGTGACC
TTTGAGTATTTGCGGGGGCGAGAGTTTGCCCCCCAGGGATCCGATTGGGAAGAGGCGGTAGCCTGGTGGAAAAGTCTGGC
CAGCGACGGCAATGCCCGCTACGACGACGTGGTGGTATTCCAAGCCGCAGACATTGCGCCGACGGTTACTTGGGGGATCA
CCCCCGGCCAAGGGATTGGGGTGGATGAGCGGATCCCTGCCCCAGAAGATCTGCCGGAAAGCGAGCGGGAACTGGCCAAA
GAGGCCTATGCCTACATGGACTTGCAGCCGGGGGATCCCATTGTAGGGACACCGGTGGATGTCTGCTTCATCGGCAGTTG
CACCAATGGCCGCTTGAGCGACCTGCGGGAGGCAGCCAAGATTGCCAAGGGGCGGCGGGTGGCCCCAGGGGTGAAGGCGT
TTGTGGTGCCCGGCTCAGAACGGGTAAAGCAGGAGGCAGAACAGGAGGGCTTGCGGGAGATCTTCGAGGCGGCAGGGTTT
GAGTGGCGGGATCCCGGTTGCTCCATGTGTTTGGCGATGAACCCGGATCGGCTGGTGGGGCGCCAGATCAGCGCCTCTTC
TTCCAACCGCAACTTCAAGGGCCGCCAGGGATCCCCCAGCGGGCGCACTCTGCTCATGAGCCCGGCCATGGTGGCAGCAG
CGGCAGTCAGCGGCAAGGTGGTGGATGTGCGGACACTGCTTTAA

Upstream 100 bases:

>100_bases
CAGCAGCGTTGCCCAGAGATAACGGAGGCCTGTGCCTTGACAAGCTTTTCCCCAAGTAGCACATTGAAAGTTTACAGGAC
TTGGCTGCGGGCCGAGAAAG

Downstream 100 bases:

>100_bases
CCAACCGTCTCTCAGGCATAGCGGGGACTGGATTTGAACCAGTGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTG
CTCTACCCCGCGGCGCTTTT

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MSERTLFDKVWDAHTVRILPSGQTQLFIGLHLIHEVTSPQAFAMLRERHLPVLFPERTVATVDHIIPTDNRARPFADPLA
EEMIQELERNCRQYGIRFYNSGSGRQGIVHVIAPEQGLTLPGMTIACGDSHTSTHGAFGAIAFGIGTSQVRDVLATQTLA
LSKLKVRRIEIHGKLGPGVYAKDVILHIIRKLGVKGGVGYAYEYGGSAIEAMSMEERMTLCNMSIEGGARCGYVNPDAVT
FEYLRGREFAPQGSDWEEAVAWWKSLASDGNARYDDVVVFQAADIAPTVTWGITPGQGIGVDERIPAPEDLPESERELAK
EAYAYMDLQPGDPIVGTPVDVCFIGSCTNGRLSDLREAAKIAKGRRVAPGVKAFVVPGSERVKQEAEQEGLREIFEAAGF
EWRDPGCSMCLAMNPDRLVGRQISASSSNRNFKGRQGSPSGRTLLMSPAMVAAAAVSGKVVDVRTLL

Sequences:

>Translated_467_residues
MSERTLFDKVWDAHTVRILPSGQTQLFIGLHLIHEVTSPQAFAMLRERHLPVLFPERTVATVDHIIPTDNRARPFADPLA
EEMIQELERNCRQYGIRFYNSGSGRQGIVHVIAPEQGLTLPGMTIACGDSHTSTHGAFGAIAFGIGTSQVRDVLATQTLA
LSKLKVRRIEIHGKLGPGVYAKDVILHIIRKLGVKGGVGYAYEYGGSAIEAMSMEERMTLCNMSIEGGARCGYVNPDAVT
FEYLRGREFAPQGSDWEEAVAWWKSLASDGNARYDDVVVFQAADIAPTVTWGITPGQGIGVDERIPAPEDLPESERELAK
EAYAYMDLQPGDPIVGTPVDVCFIGSCTNGRLSDLREAAKIAKGRRVAPGVKAFVVPGSERVKQEAEQEGLREIFEAAGF
EWRDPGCSMCLAMNPDRLVGRQISASSSNRNFKGRQGSPSGRTLLMSPAMVAAAAVSGKVVDVRTLL
>Mature_466_residues
SERTLFDKVWDAHTVRILPSGQTQLFIGLHLIHEVTSPQAFAMLRERHLPVLFPERTVATVDHIIPTDNRARPFADPLAE
EMIQELERNCRQYGIRFYNSGSGRQGIVHVIAPEQGLTLPGMTIACGDSHTSTHGAFGAIAFGIGTSQVRDVLATQTLAL
SKLKVRRIEIHGKLGPGVYAKDVILHIIRKLGVKGGVGYAYEYGGSAIEAMSMEERMTLCNMSIEGGARCGYVNPDAVTF
EYLRGREFAPQGSDWEEAVAWWKSLASDGNARYDDVVVFQAADIAPTVTWGITPGQGIGVDERIPAPEDLPESERELAKE
AYAYMDLQPGDPIVGTPVDVCFIGSCTNGRLSDLREAAKIAKGRRVAPGVKAFVVPGSERVKQEAEQEGLREIFEAAGFE
WRDPGCSMCLAMNPDRLVGRQISASSSNRNFKGRQGSPSGRTLLMSPAMVAAAAVSGKVVDVRTLL

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=397, Percent_Identity=28.463476070529, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI8659555, Length=453, Percent_Identity=23.841059602649, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI41352693, Length=393, Percent_Identity=25.1908396946565, Blast_Score=93, Evalue=5e-19,
Organism=Escherichia coli, GI1786259, Length=464, Percent_Identity=55.1724137931034, Blast_Score=501, Evalue=1e-143,
Organism=Escherichia coli, GI1787531, Length=369, Percent_Identity=27.10027100271, Blast_Score=90, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI25149337, Length=396, Percent_Identity=31.5656565656566, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI32564738, Length=396, Percent_Identity=31.5656565656566, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI25149342, Length=320, Percent_Identity=31.5625, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17568399, Length=460, Percent_Identity=24.3478260869565, Blast_Score=99, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6321429, Length=469, Percent_Identity=50.9594882729211, Blast_Score=470, Evalue=1e-133,
Organism=Saccharomyces cerevisiae, GI6322261, Length=408, Percent_Identity=29.1666666666667, Blast_Score=145, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6323335, Length=391, Percent_Identity=30.1790281329923, Blast_Score=134, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6320440, Length=445, Percent_Identity=26.5168539325843, Blast_Score=123, Evalue=6e-29,
Organism=Drosophila melanogaster, GI161076999, Length=393, Percent_Identity=29.7709923664122, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI281365315, Length=393, Percent_Identity=29.7709923664122, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI17864292, Length=393, Percent_Identity=29.7709923664122, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI28571643, Length=408, Percent_Identity=28.4313725490196, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24645686, Length=457, Percent_Identity=24.507658643326, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI17137564, Length=371, Percent_Identity=24.7978436657682, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 50799; Mature: 50667

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSERTLFDKVWDAHTVRILPSGQTQLFIGLHLIHEVTSPQAFAMLRERHLPVLFPERTVA
CCCCHHHHHHCCCEEEEEEECCCCEEEEHHHHHHHHCCHHHHHHHHHCCCCEECCCCHHH
TVDHIIPTDNRARPFADPLAEEMIQELERNCRQYGIRFYNSGSGRQGIVHVIAPEQGLTL
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCEEEEEECCCCCCC
PGMTIACGDSHTSTHGAFGAIAFGIGTSQVRDVLATQTLALSKLKVRRIEIHGKLGPGVY
CCEEEEECCCCCCCCCCHHHHEECCCHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCHH
AKDVILHIIRKLGVKGGVGYAYEYGGSAIEAMSMEERMTLCNMSIEGGARCGYVNPDAVT
HHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEE
FEYLRGREFAPQGSDWEEAVAWWKSLASDGNARYDDVVVFQAADIAPTVTWGITPGQGIG
HHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCEEEECCCCCCCCC
VDERIPAPEDLPESERELAKEAYAYMDLQPGDPIVGTPVDVCFIGSCTNGRLSDLREAAK
CCCCCCCCCCCCHHHHHHHHHHHHEEECCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHH
IAKGRRVAPGVKAFVVPGSERVKQEAEQEGLREIFEAAGFEWRDPGCSMCLAMNPDRLVG
HHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCHHHHC
RQISASSSNRNFKGRQGSPSGRTLLMSPAMVAAAAVSGKVVDVRTLL
CCCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHCCCEEEEEECC
>Mature Secondary Structure 
SERTLFDKVWDAHTVRILPSGQTQLFIGLHLIHEVTSPQAFAMLRERHLPVLFPERTVA
CCCHHHHHHCCCEEEEEEECCCCEEEEHHHHHHHHCCHHHHHHHHHCCCCEECCCCHHH
TVDHIIPTDNRARPFADPLAEEMIQELERNCRQYGIRFYNSGSGRQGIVHVIAPEQGLTL
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCEEEEEECCCCCCC
PGMTIACGDSHTSTHGAFGAIAFGIGTSQVRDVLATQTLALSKLKVRRIEIHGKLGPGVY
CCEEEEECCCCCCCCCCHHHHEECCCHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCHH
AKDVILHIIRKLGVKGGVGYAYEYGGSAIEAMSMEERMTLCNMSIEGGARCGYVNPDAVT
HHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEE
FEYLRGREFAPQGSDWEEAVAWWKSLASDGNARYDDVVVFQAADIAPTVTWGITPGQGIG
HHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCEEEECCCCCCCCC
VDERIPAPEDLPESERELAKEAYAYMDLQPGDPIVGTPVDVCFIGSCTNGRLSDLREAAK
CCCCCCCCCCCCHHHHHHHHHHHHEEECCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHH
IAKGRRVAPGVKAFVVPGSERVKQEAEQEGLREIFEAAGFEWRDPGCSMCLAMNPDRLVG
HHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCHHHHC
RQISASSSNRNFKGRQGSPSGRTLLMSPAMVAAAAVSGKVVDVRTLL
CCCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA