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The map label for this gene is pflB [H]

Identifier: 86607744

GI number: 86607744

Start: 256939

End: 259245

Strand: Reverse

Name: pflB [H]

Synonym: CYB_0245

Alternate gene names: 86607744

Gene position: 259245-256939 (Counterclockwise)

Preceding gene: 86607746

Following gene: 86607743

Centisome position: 8.51

GC content: 60.55

Gene sequence:

>2307_bases
ATGGTTGCCAGTGTGCAGCGTTCTGTAGCATCACCTACAGAATTGAAGGCGGAACCAAGGACAGGATCCAACGCAGCCGG
TCAAAAAGTTTTGAGCGATTTTGCAACGGGAGCCTGGCAGCGCTGCATCGACGTCAGAGACTTTATTCAGCGCAATTACA
CCCCCTACACCGGTGATGAAACCTTCTTAGCTGCAGCCACCGAGCGCACCCAGCGGCTGTGGGCCAAGGTCAAGGATTTG
ATGGCTCTGGAGCGAGAGCGAGGGATCCTAGATGCCGATACCGCTGTTCCCTCTACCATCACCAGCCACGCCCCTGGCTA
TATCGACCCAGAGCTGGAACAGATTGTGGGGCTGCAGACGGACAAACCTCTGAAGCGGGCCATCATGCCCTTTGGCGGCA
TCCGCGTGGTGGAGTCTTCCCTCAAAGCCTATGGCTACGAGCTGGATCCCCGCACCAAGGAAATTTTTACCCAGTACCGC
AAAACCCACAACGACGGCGTTTTTGACGCCTATACCGAAGAGATGCGCCGCTGCCGCCGCTCCGGGATCATCACCGGCCT
GCCCGATGCCTACGGTCGGGGTCGCATCATTGGGGACTACCGGCGGGTGGCCCTCTACGGGGTGGATCGGCTCATCGAAG
ACAAGCAGGCCCAAAAAGCCAGTCTAGACCTGGACACGATGGACGAAGAGACCATCCGCCTGCGGGAGGAGCTCTCCGAG
CAGATTAAGGCCCTACAAGAGCTGAAAGAGATGGGATCCCGCTACGGGTTTGACCTGGGCCGTCCGGCAGCCAACGCCCG
CGAAGCCATCCAATGGCTGTACCTGGCCTACCTGGCAGCCGTCAAGGAGCAAAACGGGGCGGCCATGTCCTTGGGGCGGG
TGTCCACCTTCCTGGACATCTACATCGAGCGGGATCTGCAGGCCGGGATCCTCACCGAGGAAGAGGCCCAGGAGCTCATT
GACCACTTTGTTATGAAGCTGCGCATGGTCAGGTTTTTGCGCACGCCCGAGTACAACGAGCTATTCAGCGGGGATCCCAC
CTGGGTTACCGAGTGCATCGGCGGCATGGGCCTGGATGGGCGGCCTTTGGTTACCAAAACCAGCTTCCGCATGCTGCATA
CCCTCTACAACCTTGGCCCAGCGCCCGAGCCCAACTTGACCGTGCTCTGGTCGGAGCGGCTGCCGGAAGCCTTCAAGCGC
TACTGCGCCAAGGTCTCCATTGAAACCAGCTCCATCCAGTACGAAAACGACGACTTGATGCGCCCCTACTGGGGGGATGA
CTACGGCATCGCCTGCTGTGTGTCGGCCATGCGCATCGGCAAGCAAATGCAGTTCTTTGGGGCGCGGGTGAACTTGGCCA
AGTGCCTGCTCTATGCCATCAACGGCGGTCGGGATGAGATCTCCGGCGAGCAGGTGGCTCCTGCTTTCGCCCCCATCACC
GCCGATGTCCTGGACTACGACGAGGTGTGGCCGCGCATGGCGCAGATGATGGCCTGGCTGGCCAAAACCTACGTCAACAC
CATGAACATCATCCACTACATGCACGACAAGTACTGCTACGAGCGCCTGGAGATGGCCCTGCACGACCGGGACGTGCTGC
GCACCATGGCCTTTGGGTTGGCCGGCCTGTCGGTAACAGCCGACTCCCTATCCGCCATCAAATACGCGCGGGTACGGGCC
ATCCGGGATGAGCGGGGCCTAGTGGTGGATTACGCGGTGGAGGGCGACTTCCCCAAATACGGCAACAACGACGACCGAGT
TGACTCCATTGCGGTGCAACTGGTGCAGACCTTCATGGCCGAGCTGCGCAAGCACAAGACCTACCGCAATGCCATCCCCA
CCCAGTCCATCCTCACCATCACCTCCAACGTGGTCTACGGCAAGAAAACCGGCAATACTCCCGATGGGCGGCGAGCGGGC
GAGCCCTTTGCCCCAGGGGCCAACCCCATGCACGGCCGGGACACCAAGGGGGCGGTGGCGTCGCTGGCTTCGGTGGCCAA
GCTGCCCTACGACGACGCTCTGGATGGGATCTCCAACACCTTCTCGATTGTGCCGGCGGCCCTAGGTCGCACAGCGGAGG
AGCGGGTTGCCAATCTAGTGGGGCTGCTGGATGGCTACATGCGGGATGGCGGTTTCCACCTCAACGTCAATGTTCTCAAC
CGCGAGACCCTGCTGCATGCCATGGAGCACCCGGAGCTCTACCCACAGCTCACCATCCGGGTTTCCGGCTATGCGGTGAA
CTTCATCAAGCTCACCCGCGAGCAGCAACTGGATGTCATTAACCGCACGTTCCATCAACACTGCTAA

Upstream 100 bases:

>100_bases
TGGTTCTTTAGCTCTCTTTTAGAGCCTACGTGGGGCAAAGGCCGGGGTGCTGCCTCGCCCTGTCGATAGATTATCAGGCG
TAATAGCCGGGAGGATTGTT

Downstream 100 bases:

>100_bases
AAGACCAGCCTGGGGATCCCTGAAGTCCGCCCATACCGGGGTCGATGGAAGGGATCCCCCATCGATAACCGAGGAGGCCC
GATGATCATGACTGTTTGTA

Product: formate acetyltransferase

Products: NA

Alternate protein names: Pyruvate formate-lyase [H]

Number of amino acids: Translated: 768; Mature: 768

Protein sequence:

>768_residues
MVASVQRSVASPTELKAEPRTGSNAAGQKVLSDFATGAWQRCIDVRDFIQRNYTPYTGDETFLAAATERTQRLWAKVKDL
MALERERGILDADTAVPSTITSHAPGYIDPELEQIVGLQTDKPLKRAIMPFGGIRVVESSLKAYGYELDPRTKEIFTQYR
KTHNDGVFDAYTEEMRRCRRSGIITGLPDAYGRGRIIGDYRRVALYGVDRLIEDKQAQKASLDLDTMDEETIRLREELSE
QIKALQELKEMGSRYGFDLGRPAANAREAIQWLYLAYLAAVKEQNGAAMSLGRVSTFLDIYIERDLQAGILTEEEAQELI
DHFVMKLRMVRFLRTPEYNELFSGDPTWVTECIGGMGLDGRPLVTKTSFRMLHTLYNLGPAPEPNLTVLWSERLPEAFKR
YCAKVSIETSSIQYENDDLMRPYWGDDYGIACCVSAMRIGKQMQFFGARVNLAKCLLYAINGGRDEISGEQVAPAFAPIT
ADVLDYDEVWPRMAQMMAWLAKTYVNTMNIIHYMHDKYCYERLEMALHDRDVLRTMAFGLAGLSVTADSLSAIKYARVRA
IRDERGLVVDYAVEGDFPKYGNNDDRVDSIAVQLVQTFMAELRKHKTYRNAIPTQSILTITSNVVYGKKTGNTPDGRRAG
EPFAPGANPMHGRDTKGAVASLASVAKLPYDDALDGISNTFSIVPAALGRTAEERVANLVGLLDGYMRDGGFHLNVNVLN
RETLLHAMEHPELYPQLTIRVSGYAVNFIKLTREQQLDVINRTFHQHC

Sequences:

>Translated_768_residues
MVASVQRSVASPTELKAEPRTGSNAAGQKVLSDFATGAWQRCIDVRDFIQRNYTPYTGDETFLAAATERTQRLWAKVKDL
MALERERGILDADTAVPSTITSHAPGYIDPELEQIVGLQTDKPLKRAIMPFGGIRVVESSLKAYGYELDPRTKEIFTQYR
KTHNDGVFDAYTEEMRRCRRSGIITGLPDAYGRGRIIGDYRRVALYGVDRLIEDKQAQKASLDLDTMDEETIRLREELSE
QIKALQELKEMGSRYGFDLGRPAANAREAIQWLYLAYLAAVKEQNGAAMSLGRVSTFLDIYIERDLQAGILTEEEAQELI
DHFVMKLRMVRFLRTPEYNELFSGDPTWVTECIGGMGLDGRPLVTKTSFRMLHTLYNLGPAPEPNLTVLWSERLPEAFKR
YCAKVSIETSSIQYENDDLMRPYWGDDYGIACCVSAMRIGKQMQFFGARVNLAKCLLYAINGGRDEISGEQVAPAFAPIT
ADVLDYDEVWPRMAQMMAWLAKTYVNTMNIIHYMHDKYCYERLEMALHDRDVLRTMAFGLAGLSVTADSLSAIKYARVRA
IRDERGLVVDYAVEGDFPKYGNNDDRVDSIAVQLVQTFMAELRKHKTYRNAIPTQSILTITSNVVYGKKTGNTPDGRRAG
EPFAPGANPMHGRDTKGAVASLASVAKLPYDDALDGISNTFSIVPAALGRTAEERVANLVGLLDGYMRDGGFHLNVNVLN
RETLLHAMEHPELYPQLTIRVSGYAVNFIKLTREQQLDVINRTFHQHC
>Mature_768_residues
MVASVQRSVASPTELKAEPRTGSNAAGQKVLSDFATGAWQRCIDVRDFIQRNYTPYTGDETFLAAATERTQRLWAKVKDL
MALERERGILDADTAVPSTITSHAPGYIDPELEQIVGLQTDKPLKRAIMPFGGIRVVESSLKAYGYELDPRTKEIFTQYR
KTHNDGVFDAYTEEMRRCRRSGIITGLPDAYGRGRIIGDYRRVALYGVDRLIEDKQAQKASLDLDTMDEETIRLREELSE
QIKALQELKEMGSRYGFDLGRPAANAREAIQWLYLAYLAAVKEQNGAAMSLGRVSTFLDIYIERDLQAGILTEEEAQELI
DHFVMKLRMVRFLRTPEYNELFSGDPTWVTECIGGMGLDGRPLVTKTSFRMLHTLYNLGPAPEPNLTVLWSERLPEAFKR
YCAKVSIETSSIQYENDDLMRPYWGDDYGIACCVSAMRIGKQMQFFGARVNLAKCLLYAINGGRDEISGEQVAPAFAPIT
ADVLDYDEVWPRMAQMMAWLAKTYVNTMNIIHYMHDKYCYERLEMALHDRDVLRTMAFGLAGLSVTADSLSAIKYARVRA
IRDERGLVVDYAVEGDFPKYGNNDDRVDSIAVQLVQTFMAELRKHKTYRNAIPTQSILTITSNVVYGKKTGNTPDGRRAG
EPFAPGANPMHGRDTKGAVASLASVAKLPYDDALDGISNTFSIVPAALGRTAEERVANLVGLLDGYMRDGGFHLNVNVLN
RETLLHAMEHPELYPQLTIRVSGYAVNFIKLTREQQLDVINRTFHQHC

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1787131, Length=744, Percent_Identity=68.5483870967742, Blast_Score=1060, Evalue=0.0,
Organism=Escherichia coli, GI48994926, Length=744, Percent_Identity=66.6666666666667, Blast_Score=1013, Evalue=0.0,
Organism=Escherichia coli, GI1787044, Length=748, Percent_Identity=25.2673796791444, Blast_Score=186, Evalue=6e-48,
Organism=Escherichia coli, GI1790388, Length=670, Percent_Identity=25.8208955223881, Blast_Score=160, Evalue=4e-40,
Organism=Escherichia coli, GI1788933, Length=58, Percent_Identity=72.4137931034483, Blast_Score=85, Evalue=1e-17,

Paralogues:

None

Copy number: 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005949
- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 86368; Mature: 86368

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVASVQRSVASPTELKAEPRTGSNAAGQKVLSDFATGAWQRCIDVRDFIQRNYTPYTGDE
CCCCHHHHHCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
TFLAAATERTQRLWAKVKDLMALERERGILDADTAVPSTITSHAPGYIDPELEQIVGLQT
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHCCCC
DKPLKRAIMPFGGIRVVESSLKAYGYELDPRTKEIFTQYRKTHNDGVFDAYTEEMRRCRR
CCHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
SGIITGLPDAYGRGRIIGDYRRVALYGVDRLIEDKQAQKASLDLDTMDEETIRLREELSE
CCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
QIKALQELKEMGSRYGFDLGRPAANAREAIQWLYLAYLAAVKEQNGAAMSLGRVSTFLDI
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHH
YIERDLQAGILTEEEAQELIDHFVMKLRMVRFLRTPEYNELFSGDPTWVTECIGGMGLDG
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCHHHHHHHCCCCCCC
RPLVTKTSFRMLHTLYNLGPAPEPNLTVLWSERLPEAFKRYCAKVSIETSSIQYENDDLM
CCCCHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHEEEECCCEEECCCCCC
RPYWGDDYGIACCVSAMRIGKQMQFFGARVNLAKCLLYAINGGRDEISGEQVAPAFAPIT
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHCCCH
ADVLDYDEVWPRMAQMMAWLAKTYVNTMNIIHYMHDKYCYERLEMALHDRDVLRTMAFGL
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGLSVTADSLSAIKYARVRAIRDERGLVVDYAVEGDFPKYGNNDDRVDSIAVQLVQTFMA
HCCEECHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHH
ELRKHKTYRNAIPTQSILTITSNVVYGKKTGNTPDGRRAGEPFAPGANPMHGRDTKGAVA
HHHHHHHHHHCCCCHHHHHHHHCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
SLASVAKLPYDDALDGISNTFSIVPAALGRTAEERVANLVGLLDGYMRDGGFHLNVNVLN
HHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEC
RETLLHAMEHPELYPQLTIRVSGYAVNFIKLTREQQLDVINRTFHQHC
HHHHHHHHHCCCCCCEEEEEEECEEEEHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVASVQRSVASPTELKAEPRTGSNAAGQKVLSDFATGAWQRCIDVRDFIQRNYTPYTGDE
CCCCHHHHHCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
TFLAAATERTQRLWAKVKDLMALERERGILDADTAVPSTITSHAPGYIDPELEQIVGLQT
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHCCCC
DKPLKRAIMPFGGIRVVESSLKAYGYELDPRTKEIFTQYRKTHNDGVFDAYTEEMRRCRR
CCHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
SGIITGLPDAYGRGRIIGDYRRVALYGVDRLIEDKQAQKASLDLDTMDEETIRLREELSE
CCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
QIKALQELKEMGSRYGFDLGRPAANAREAIQWLYLAYLAAVKEQNGAAMSLGRVSTFLDI
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHH
YIERDLQAGILTEEEAQELIDHFVMKLRMVRFLRTPEYNELFSGDPTWVTECIGGMGLDG
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCHHHHHHHCCCCCCC
RPLVTKTSFRMLHTLYNLGPAPEPNLTVLWSERLPEAFKRYCAKVSIETSSIQYENDDLM
CCCCHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHEEEECCCEEECCCCCC
RPYWGDDYGIACCVSAMRIGKQMQFFGARVNLAKCLLYAINGGRDEISGEQVAPAFAPIT
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHCCCH
ADVLDYDEVWPRMAQMMAWLAKTYVNTMNIIHYMHDKYCYERLEMALHDRDVLRTMAFGL
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGLSVTADSLSAIKYARVRAIRDERGLVVDYAVEGDFPKYGNNDDRVDSIAVQLVQTFMA
HCCEECHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHH
ELRKHKTYRNAIPTQSILTITSNVVYGKKTGNTPDGRRAGEPFAPGANPMHGRDTKGAVA
HHHHHHHHHHCCCCHHHHHHHHCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
SLASVAKLPYDDALDGISNTFSIVPAALGRTAEERVANLVGLLDGYMRDGGFHLNVNVLN
HHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEC
RETLLHAMEHPELYPQLTIRVSGYAVNFIKLTREQQLDVINRTFHQHC
HHHHHHHHHCCCCCCEEEEEEECEEEEHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA