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The map label for this gene is narB [H]

Identifier: 86607542

GI number: 86607542

Start: 49774

End: 51909

Strand: Direct

Name: narB [H]

Synonym: CYB_0040

Alternate gene names: 86607542

Gene position: 49774-51909 (Clockwise)

Preceding gene: 86607541

Following gene: 86607543

Centisome position: 1.63

GC content: 56.51

Gene sequence:

>2136_bases
ATGGAGCCGGAAAAAGCCGTCATCAAAACCCTATGTCCCTACTGCGGCGTAGGGTGTGGATTGGAAGCGGTTTCGACAGC
CTCAGGTGGCTGGAAAATAAGAGGCGACCGGGAGCATCCTTCCAGCTTGGGGATGGTCTGTGTAAAGGGGGCTACGGTTC
TGGAGGCAGTTGGTCGAGATCGGCTGCTCTATCCCCTGTTTCGGGAGAGCTTGGGCCAGCCCTTTCGTCGCATTTCTTGG
GATCAGGCTTTTGAGGAGATGGTTTCTCGGCTGCGCTGCCTCAAAGACAACCCTCACTCCATGGCTATGTATGGATCGGG
GCAGTTGCTCACTGAAGACTATTACGTTGCTCAAAAGCTCATTAAGGGCTGCCTGGGCACCAACAATTTCGACACCAACT
CGCGCCTGTGCATGTCCTCAGCCGTCTCGGCCTACACCCAATCCCTGGGATCCGATGGCCCCCCCTGCTGCTACGAGGAT
CTCGATCTCTGCAATCTGGCTTTTCTGGTGGGCACCAACACCGCCGAGTGCCATCCCATTCTTTTTAATCGCTTGAAGAA
GCGGCACAAGCCCGGATCCCCCCTCAAAATGGTGGTCGTGGATCCCCGGCGCACTCAGACAGCAGAAGCTGCCGATCTCC
ACTTAAAAATTCGCCCCGGTACGGATATCGATCTTTTCAATGGGATTGCCCATTTGTTGTTAAAGTGGGGATCCATTGCT
GCTGATTTTATCGAGGGCTGCACCCAAGGTTTTGCTGAATATGCAGCTTTGCTGGCCGATTATGAACCGCAGATTGTGGC
CCAGCGCTGCGGAATTGCCACCGAGGATTTGATCGAAGCAGCCAAGCTTTGGCAGGGATCCCAAAGGGTTCTCTCCCTTT
GGTCGATGGGCCTAAACCAGTCCAGTGAGGGCACCGCCAAGATCACCAGCTTAATCAACCTGCATCTCCTGACCGGGCAA
ATTGGCCGACCGGGGACGGGCCCCTTTTCCTTGACCGGCCAGCCCAATGCCATGGGCGGTCGAGAGGCAGGGGGGTTATC
CCATCTGCTGCCGGGATATCGGCTGGTGAGCCAGCCCCAGCATCGGGCAGAGGTGGAGCAGGCTTGGGGTTTGCCCGGCG
GCTCCATCTCCCCCCACCCAGGACTGAGGGCCACCGAGATGATTGCCGCTCTGGAGACGGGATCCCTGCAGTTCTTCTGG
ATTGTGGCCACTAATCCTTTGGTGAGCTTTCCGGATCTGGAACGGGTCAAAAAAGCCCTGAAAAAGTCTCCCTTTACTGT
TTATCAAGAGGCTTATTACCCTACAGAAACGGCAGAATATGCCCATTTGCTTTTGCCTGCCTGTCAGTGGAGCGAGAAAA
CCGGGGTGATGACCAACTCCGAGCGCAGGGTTACCCTTTGTCAAGCCTTTCGAGATCCCCCTGGCGAAGCCCGACCCGAT
TGGGAGATCTTTGCTGAAGTGGGGCGCAGGTTGGGCTTTGAAGCGCAATTTTCCTTCCAAACCGCGGCAGAGGTTTATGC
AGAATTTGCCGCCCTTACCGCAAATCGCCTCTGCGATCACTCCGGCCTCAGCCATGCCCTTTTGGCAGAACGAGGCCCTC
AGCAATGGCCTTATCCCAAAGAAAGAACCTCGGCCCCTAAGTCCACTCGCCTCTACACAGATTGGCGTTTCCCGACCCCC
GATGGCCGCGCCCGCTTTATCCCCCACCATTCCCGTGGCCTAGCAGAGCCGCCGGATCCCCAGTTTCCCTACGTATTAAC
CACGGGCAGGCTTTACGGCCATTGGCACACCCAAACCCGCACCGGCAGGATTGAAAAAATCAAGCAAATGCACCCCCAAC
CCTTTATTGAAATCCATCCTCGCGATGCGGAAAAGCTGGGCATCCAAGATGGAGATTGGGTTCAAGTGCGTTCTGCCCGC
GGGCGGGCCTATTTCCCGGCCAAGATAACTGCCAACATCGCCCCTGGAACAGTGTTTGTGCCCATGCACTGGGGATCCCT
CTGGGCAGAACAAGCCGAGTGTAATGCCCTTACCCATGACATCAGTTGTCCCGATTCCGGCCAGCCGGAATTAAAAGCCT
GTGCCGTTGCTCTTGAGAAAGCCGACCCACCGCTCAAGCAATCCCCTCAACTCTAG

Upstream 100 bases:

>100_bases
ATCTCCTCGGCTGAGAGATCCTTAACCTGATGGCCTATCGGGATCCAAAATGCAAGACACTTATCCATCCTGACAGGGAT
CCCCACTCATGGTGACCGCG

Downstream 100 bases:

>100_bases
ATTTGGTTCCTAATCGAGGTTCCCATGACTCTGTTTCAGTTTGAGCAAGATTTTGCCGACTCTCTGCGATGCATCCCCAT
GACCTTGCGCCTAAAGCTGG

Product: nitrate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 711; Mature: 711

Protein sequence:

>711_residues
MEPEKAVIKTLCPYCGVGCGLEAVSTASGGWKIRGDREHPSSLGMVCVKGATVLEAVGRDRLLYPLFRESLGQPFRRISW
DQAFEEMVSRLRCLKDNPHSMAMYGSGQLLTEDYYVAQKLIKGCLGTNNFDTNSRLCMSSAVSAYTQSLGSDGPPCCYED
LDLCNLAFLVGTNTAECHPILFNRLKKRHKPGSPLKMVVVDPRRTQTAEAADLHLKIRPGTDIDLFNGIAHLLLKWGSIA
ADFIEGCTQGFAEYAALLADYEPQIVAQRCGIATEDLIEAAKLWQGSQRVLSLWSMGLNQSSEGTAKITSLINLHLLTGQ
IGRPGTGPFSLTGQPNAMGGREAGGLSHLLPGYRLVSQPQHRAEVEQAWGLPGGSISPHPGLRATEMIAALETGSLQFFW
IVATNPLVSFPDLERVKKALKKSPFTVYQEAYYPTETAEYAHLLLPACQWSEKTGVMTNSERRVTLCQAFRDPPGEARPD
WEIFAEVGRRLGFEAQFSFQTAAEVYAEFAALTANRLCDHSGLSHALLAERGPQQWPYPKERTSAPKSTRLYTDWRFPTP
DGRARFIPHHSRGLAEPPDPQFPYVLTTGRLYGHWHTQTRTGRIEKIKQMHPQPFIEIHPRDAEKLGIQDGDWVQVRSAR
GRAYFPAKITANIAPGTVFVPMHWGSLWAEQAECNALTHDISCPDSGQPELKACAVALEKADPPLKQSPQL

Sequences:

>Translated_711_residues
MEPEKAVIKTLCPYCGVGCGLEAVSTASGGWKIRGDREHPSSLGMVCVKGATVLEAVGRDRLLYPLFRESLGQPFRRISW
DQAFEEMVSRLRCLKDNPHSMAMYGSGQLLTEDYYVAQKLIKGCLGTNNFDTNSRLCMSSAVSAYTQSLGSDGPPCCYED
LDLCNLAFLVGTNTAECHPILFNRLKKRHKPGSPLKMVVVDPRRTQTAEAADLHLKIRPGTDIDLFNGIAHLLLKWGSIA
ADFIEGCTQGFAEYAALLADYEPQIVAQRCGIATEDLIEAAKLWQGSQRVLSLWSMGLNQSSEGTAKITSLINLHLLTGQ
IGRPGTGPFSLTGQPNAMGGREAGGLSHLLPGYRLVSQPQHRAEVEQAWGLPGGSISPHPGLRATEMIAALETGSLQFFW
IVATNPLVSFPDLERVKKALKKSPFTVYQEAYYPTETAEYAHLLLPACQWSEKTGVMTNSERRVTLCQAFRDPPGEARPD
WEIFAEVGRRLGFEAQFSFQTAAEVYAEFAALTANRLCDHSGLSHALLAERGPQQWPYPKERTSAPKSTRLYTDWRFPTP
DGRARFIPHHSRGLAEPPDPQFPYVLTTGRLYGHWHTQTRTGRIEKIKQMHPQPFIEIHPRDAEKLGIQDGDWVQVRSAR
GRAYFPAKITANIAPGTVFVPMHWGSLWAEQAECNALTHDISCPDSGQPELKACAVALEKADPPLKQSPQL
>Mature_711_residues
MEPEKAVIKTLCPYCGVGCGLEAVSTASGGWKIRGDREHPSSLGMVCVKGATVLEAVGRDRLLYPLFRESLGQPFRRISW
DQAFEEMVSRLRCLKDNPHSMAMYGSGQLLTEDYYVAQKLIKGCLGTNNFDTNSRLCMSSAVSAYTQSLGSDGPPCCYED
LDLCNLAFLVGTNTAECHPILFNRLKKRHKPGSPLKMVVVDPRRTQTAEAADLHLKIRPGTDIDLFNGIAHLLLKWGSIA
ADFIEGCTQGFAEYAALLADYEPQIVAQRCGIATEDLIEAAKLWQGSQRVLSLWSMGLNQSSEGTAKITSLINLHLLTGQ
IGRPGTGPFSLTGQPNAMGGREAGGLSHLLPGYRLVSQPQHRAEVEQAWGLPGGSISPHPGLRATEMIAALETGSLQFFW
IVATNPLVSFPDLERVKKALKKSPFTVYQEAYYPTETAEYAHLLLPACQWSEKTGVMTNSERRVTLCQAFRDPPGEARPD
WEIFAEVGRRLGFEAQFSFQTAAEVYAEFAALTANRLCDHSGLSHALLAERGPQQWPYPKERTSAPKSTRLYTDWRFPTP
DGRARFIPHHSRGLAEPPDPQFPYVLTTGRLYGHWHTQTRTGRIEKIKQMHPQPFIEIHPRDAEKLGIQDGDWVQVRSAR
GRAYFPAKITANIAPGTVFVPMHWGSLWAEQAECNALTHDISCPDSGQPELKACAVALEKADPPLKQSPQL

Specific function: Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria [H]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788534, Length=792, Percent_Identity=30.5555555555556, Blast_Score=338, Evalue=7e-94,
Organism=Escherichia coli, GI3868721, Length=703, Percent_Identity=28.4495021337127, Blast_Score=331, Evalue=8e-92,
Organism=Escherichia coli, GI3868719, Length=622, Percent_Identity=24.5980707395498, Blast_Score=159, Evalue=4e-40,
Organism=Escherichia coli, GI3868720, Length=621, Percent_Identity=23.8325281803543, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1787778, Length=692, Percent_Identity=24.7109826589595, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1787870, Length=747, Percent_Identity=24.6318607764391, Blast_Score=112, Evalue=1e-25,
Organism=Escherichia coli, GI145693196, Length=517, Percent_Identity=25.9187620889749, Blast_Score=109, Evalue=8e-25,
Organism=Escherichia coli, GI87081797, Length=693, Percent_Identity=23.2323232323232, Blast_Score=106, Evalue=5e-24,
Organism=Escherichia coli, GI171474008, Length=744, Percent_Identity=22.5806451612903, Blast_Score=105, Evalue=7e-24,
Organism=Escherichia coli, GI87081994, Length=716, Percent_Identity=25, Blast_Score=99, Evalue=8e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 78536; Mature: 78536

Theoretical pI: Translated: 7.38; Mature: 7.38

Prosite motif: PS00932 MOLYBDOPTERIN_PROK_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPEKAVIKTLCPYCGVGCGLEAVSTASGGWKIRGDREHPSSLGMVCVKGATVLEAVGRD
CCCHHHHHHHHHHHHCCCCCHHHHHCCCCCEEEECCCCCCCCCCEEEECCHHHHHHHCCC
RLLYPLFRESLGQPFRRISWDQAFEEMVSRLRCLKDNPHSMAMYGSGQLLTEDYYVAQKL
CEEHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEHHHHHHHHHH
IKGCLGTNNFDTNSRLCMSSAVSAYTQSLGSDGPPCCYEDLDLCNLAFLVGTNTAECHPI
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHEEEEEECCCCCCHHH
LFNRLKKRHKPGSPLKMVVVDPRRTQTAEAADLHLKIRPGTDIDLFNGIAHLLLKWGSIA
HHHHHHHHCCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
ADFIEGCTQGFAEYAALLADYEPQIVAQRCGIATEDLIEAAKLWQGSQRVLSLWSMGLNQ
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCC
SSEGTAKITSLINLHLLTGQIGRPGTGPFSLTGQPNAMGGREAGGLSHLLPGYRLVSQPQ
CCCCHHHHHHHHHHHEEECCCCCCCCCCEEECCCCCCCCCCCCCCHHHHCCCCHHHCCCH
HRAEVEQAWGLPGGSISPHPGLRATEMIAALETGSLQFFWIVATNPLVSFPDLERVKKAL
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCCCHHHHHHHH
KKSPFTVYQEAYYPTETAEYAHLLLPACQWSEKTGVMTNSERRVTLCQAFRDPPGEARPD
HCCCCEEEHHCCCCCCHHHHHHHHHHHHCCCCCCCCEECCCCHHHHHHHHCCCCCCCCCC
WEIFAEVGRRLGFEAQFSFQTAAEVYAEFAALTANRLCDHSGLSHALLAERGPQQWPYPK
HHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCH
ERTSAPKSTRLYTDWRFPTPDGRARFIPHHSRGLAEPPDPQFPYVLTTGRLYGHWHTQTR
HHCCCCCCCEEEEECCCCCCCCCEEECCCCCCCCCCCCCCCCCEEEECCEEEEEEECCCC
TGRIEKIKQMHPQPFIEIHPRDAEKLGIQDGDWVQVRSARGRAYFPAKITANIAPGTVFV
CCHHHHHHHHCCCCEEEECCCCHHHCCCCCCCEEEEECCCCCEECCCEEEEECCCCEEEE
PMHWGSLWAEQAECNALTHDISCPDSGQPELKACAVALEKADPPLKQSPQL
EECCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MEPEKAVIKTLCPYCGVGCGLEAVSTASGGWKIRGDREHPSSLGMVCVKGATVLEAVGRD
CCCHHHHHHHHHHHHCCCCCHHHHHCCCCCEEEECCCCCCCCCCEEEECCHHHHHHHCCC
RLLYPLFRESLGQPFRRISWDQAFEEMVSRLRCLKDNPHSMAMYGSGQLLTEDYYVAQKL
CEEHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEHHHHHHHHHH
IKGCLGTNNFDTNSRLCMSSAVSAYTQSLGSDGPPCCYEDLDLCNLAFLVGTNTAECHPI
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHEEEEEECCCCCCHHH
LFNRLKKRHKPGSPLKMVVVDPRRTQTAEAADLHLKIRPGTDIDLFNGIAHLLLKWGSIA
HHHHHHHHCCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
ADFIEGCTQGFAEYAALLADYEPQIVAQRCGIATEDLIEAAKLWQGSQRVLSLWSMGLNQ
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCC
SSEGTAKITSLINLHLLTGQIGRPGTGPFSLTGQPNAMGGREAGGLSHLLPGYRLVSQPQ
CCCCHHHHHHHHHHHEEECCCCCCCCCCEEECCCCCCCCCCCCCCHHHHCCCCHHHCCCH
HRAEVEQAWGLPGGSISPHPGLRATEMIAALETGSLQFFWIVATNPLVSFPDLERVKKAL
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCCCHHHHHHHH
KKSPFTVYQEAYYPTETAEYAHLLLPACQWSEKTGVMTNSERRVTLCQAFRDPPGEARPD
HCCCCEEEHHCCCCCCHHHHHHHHHHHHCCCCCCCCEECCCCHHHHHHHHCCCCCCCCCC
WEIFAEVGRRLGFEAQFSFQTAAEVYAEFAALTANRLCDHSGLSHALLAERGPQQWPYPK
HHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCH
ERTSAPKSTRLYTDWRFPTPDGRARFIPHHSRGLAEPPDPQFPYVLTTGRLYGHWHTQTR
HHCCCCCCCEEEEECCCCCCCCCEEECCCCCCCCCCCCCCCCCEEEECCEEEEEEECCCC
TGRIEKIKQMHPQPFIEIHPRDAEKLGIQDGDWVQVRSARGRAYFPAKITANIAPGTVFV
CCHHHHHHHHCCCCEEEECCCCHHHCCCCCCCEEEEECCCCCEECCCEEEEECCCCEEEE
PMHWGSLWAEQAECNALTHDISCPDSGQPELKACAVALEKADPPLKQSPQL
EECCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]