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The map label for this gene is nirA [H]

Identifier: 86607536

GI number: 86607536

Start: 42092

End: 43639

Strand: Direct

Name: nirA [H]

Synonym: CYB_0034

Alternate gene names: 86607536

Gene position: 42092-43639 (Clockwise)

Preceding gene: 86607535

Following gene: 86607537

Centisome position: 1.38

GC content: 62.6

Gene sequence:

>1548_bases
ATGGCGAACCAATTTGAACGCCTCAAAAGCGAAAAGGATGGGCTGGCGGTCAAGGCCGAGCTGGAGGCGTTTGCCCGGAT
GGGTTGGGAGAACATTCCTGAAGACGACCGGGATCACCGCCTCAAGTGGCTGGGGATCTTCTTTCGCAAGCGCACCCCAG
GTCAGTTCATGCTGCGGCTGCGCCTGCCCAATGGGATCCTAACCAGCGGCCAAATGCGGATGTTGGGCGCAATCATCCAC
CCCTATGGAGAACAGGGCGTAGCCGACATCACCACCCGGCAGAACCTGCAACTGCGGGGCATCCCCATCGAGGAAATGCC
CCAGATCCTGGGCTACCTGAAAGAGGTAGGCCTGACCAGCATCCAGTCGGGCATGGACAACGTGCGCAACATCACGGGAT
CCCCTCTGGCCGGTATTGACCCGGACGAGCTGATCGATGTGCGCGGTCTCACCCGCAAGGTGCAGGACATGATCACCAAC
AACGGCGAGGGCAACCCTTCCTTCAGCAACCTGCCGCGCAAGTTCAACATCGCCATCTGCGGTTGTCGCGACAACTCCGT
GCATGCGGAGATCAACGACCTGGCCTTTGTGCCCGCCTTCAAAAATGGCCGCCTGGGCTTCAACGTCCTGGTGGGCGGCT
TTTTCTCGGCTCGCCGCTGCGCCGAGGCAATTGGCCTAGATGTCTGGGTGGATCCCCGCGATGTGGTTCCTCTGTGCGAG
GCGGTGCTGCTGGTCTACCGGGATCACGGCCTGCGGGCCAACCGGCAAAAGGCGCGGTTGATGTGGCTCATTGACGAGTG
GGGCCTAGAGAAGTTCCGGGCGGCTGTGGAGCGCCAGACTGGCCACCCTCTGCTCAGGGCAGCGGAAAAAGATGAGGTGG
TCTGGCACAAGCGGGATCTGCTGGGGGTGCATGCCCAGAAGCAGCCGGGCCTCAACTTTGTCGGCCTGCACGTGCCTGTG
GGGCGGCTCAACGCCCTGGAGATGATGGAGCTGGCCCGCTTGGCGGAGGTGTACGGCTCCGGGGAGCTGCGGCTGACGGT
GGAGCAGAACGTGCTCATCCCCAATGTGCCCGACTCCCGAGTGGCCCCGCTCCTCAAAGAGCCGCTCTTGAAGAAGTTCT
CCCCCAACCCAGGGCCCTTGCAGCGGGGGTTGGTGTCCTGTACGGGCAACCAGTTCTGCAACTTTGCCCTTATCGAGACC
AAAAACCGGGCTGTGGCCTTGATGGAGGAGCTGGAGGCGGAGCTGGAGATCCCCCAAACGGTGCGCATCCACTGGACGGG
CTGCCCCAACTCCTGCGGCCAACCCCAAGTAGCCGATATCGGCCTTATGGGCACCACTGCTCGCAAGGACGGCAGGGTGG
TGGAGGCCGTGGACATCTACATGGGGGGAGAGGTGGGCAAAGACGCCAAGCTGGGCGAATGCGTGCGCAAAGGGATCCCT
TGCGAAGACCTCAAGCCGGTCTTGGTGGAGTTGCTCAGGGAGCACTTTGGGGCCAGGCCGCGTCAGCATCCGTCCGCCGC
CCAGGCTTCTGTTTTGGTAACCCGCTAG

Upstream 100 bases:

>100_bases
TGACAAAACTGCATAACCTGTTACAGAGTGTAACTGCCAATACCAAAGTTAACAGTTCGTCATCGCTAGAGTAGCCAGTG
TTCACCCCACAGCAGCGATC

Downstream 100 bases:

>100_bases
CGGTTCTGTTGCCGTCCTGAAAGTTTTTCTTTCTTCGTCGTTGTAAATCTTGGAGGTTTCGATGTCTAAGCTGTTCAGCA
CTTCCTCTCCCGTCTCCCGT

Product: ferredoxin-nitrite reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 515; Mature: 514

Protein sequence:

>515_residues
MANQFERLKSEKDGLAVKAELEAFARMGWENIPEDDRDHRLKWLGIFFRKRTPGQFMLRLRLPNGILTSGQMRMLGAIIH
PYGEQGVADITTRQNLQLRGIPIEEMPQILGYLKEVGLTSIQSGMDNVRNITGSPLAGIDPDELIDVRGLTRKVQDMITN
NGEGNPSFSNLPRKFNIAICGCRDNSVHAEINDLAFVPAFKNGRLGFNVLVGGFFSARRCAEAIGLDVWVDPRDVVPLCE
AVLLVYRDHGLRANRQKARLMWLIDEWGLEKFRAAVERQTGHPLLRAAEKDEVVWHKRDLLGVHAQKQPGLNFVGLHVPV
GRLNALEMMELARLAEVYGSGELRLTVEQNVLIPNVPDSRVAPLLKEPLLKKFSPNPGPLQRGLVSCTGNQFCNFALIET
KNRAVALMEELEAELEIPQTVRIHWTGCPNSCGQPQVADIGLMGTTARKDGRVVEAVDIYMGGEVGKDAKLGECVRKGIP
CEDLKPVLVELLREHFGARPRQHPSAAQASVLVTR

Sequences:

>Translated_515_residues
MANQFERLKSEKDGLAVKAELEAFARMGWENIPEDDRDHRLKWLGIFFRKRTPGQFMLRLRLPNGILTSGQMRMLGAIIH
PYGEQGVADITTRQNLQLRGIPIEEMPQILGYLKEVGLTSIQSGMDNVRNITGSPLAGIDPDELIDVRGLTRKVQDMITN
NGEGNPSFSNLPRKFNIAICGCRDNSVHAEINDLAFVPAFKNGRLGFNVLVGGFFSARRCAEAIGLDVWVDPRDVVPLCE
AVLLVYRDHGLRANRQKARLMWLIDEWGLEKFRAAVERQTGHPLLRAAEKDEVVWHKRDLLGVHAQKQPGLNFVGLHVPV
GRLNALEMMELARLAEVYGSGELRLTVEQNVLIPNVPDSRVAPLLKEPLLKKFSPNPGPLQRGLVSCTGNQFCNFALIET
KNRAVALMEELEAELEIPQTVRIHWTGCPNSCGQPQVADIGLMGTTARKDGRVVEAVDIYMGGEVGKDAKLGECVRKGIP
CEDLKPVLVELLREHFGARPRQHPSAAQASVLVTR
>Mature_514_residues
ANQFERLKSEKDGLAVKAELEAFARMGWENIPEDDRDHRLKWLGIFFRKRTPGQFMLRLRLPNGILTSGQMRMLGAIIHP
YGEQGVADITTRQNLQLRGIPIEEMPQILGYLKEVGLTSIQSGMDNVRNITGSPLAGIDPDELIDVRGLTRKVQDMITNN
GEGNPSFSNLPRKFNIAICGCRDNSVHAEINDLAFVPAFKNGRLGFNVLVGGFFSARRCAEAIGLDVWVDPRDVVPLCEA
VLLVYRDHGLRANRQKARLMWLIDEWGLEKFRAAVERQTGHPLLRAAEKDEVVWHKRDLLGVHAQKQPGLNFVGLHVPVG
RLNALEMMELARLAEVYGSGELRLTVEQNVLIPNVPDSRVAPLLKEPLLKKFSPNPGPLQRGLVSCTGNQFCNFALIETK
NRAVALMEELEAELEIPQTVRIHWTGCPNSCGQPQVADIGLMGTTARKDGRVVEAVDIYMGGEVGKDAKLGECVRKGIPC
EDLKPVLVELLREHFGARPRQHPSAAQASVLVTR

Specific function: This Enzyme Catalyzes The 6-Electron Reduction Of Sulfite To Sulfide. This Is One Of Several Activities Required For The Biosynthesis Of L-Cysteine From Sulfate. [C]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Escherichia coli, GI1789122, Length=450, Percent_Identity=25.7777777777778, Blast_Score=117, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6322597, Length=527, Percent_Identity=24.0986717267552, Blast_Score=100, Evalue=4e-22,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.7.7.1 [H]

Molecular weight: Translated: 57274; Mature: 57143

Theoretical pI: Translated: 7.94; Mature: 7.94

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANQFERLKSEKDGLAVKAELEAFARMGWENIPEDDRDHRLKWLGIFFRKRTPGQFMLRL
CCCHHHHHHCCCCCCEEEHHHHHHHHCCCCCCCCCCHHCCEEEEEEEEECCCCCEEEEEE
RLPNGILTSGQMRMLGAIIHPYGEQGVADITTRQNLQLRGIPIEEMPQILGYLKEVGLTS
ECCCCCCCCCHHHHHHHHHCCCCCCCCEECCCCCCCEEECCCHHHHHHHHHHHHHHCHHH
IQSGMDNVRNITGSPLAGIDPDELIDVRGLTRKVQDMITNNGEGNPSFSNLPRKFNIAIC
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCEEEEEEE
GCRDNSVHAEINDLAFVPAFKNGRLGFNVLVGGFFSARRCAEAIGLDVWVDPRDVVPLCE
ECCCCCCEEEECCEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHH
AVLLVYRDHGLRANRQKARLMWLIDEWGLEKFRAAVERQTGHPLLRAAEKDEVVWHKRDL
HHHHHHHCCCCCCCCCCCEEEEEHHHCCHHHHHHHHHHCCCCHHHHCCCCCCEEEEHHHH
LGVHAQKQPGLNFVGLHVPVGRLNALEMMELARLAEVYGSGELRLTVEQNVLIPNVPDSR
HCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCEECCCCCCHH
VAPLLKEPLLKKFSPNPGPLQRGLVSCTGNQFCNFALIETKNRAVALMEELEAELEIPQT
HHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCE
VRIHWTGCPNSCGQPQVADIGLMGTTARKDGRVVEAVDIYMGGEVGKDAKLGECVRKGIP
EEEEEECCCCCCCCCCEEEEEEECCCCCCCCCEEEEEEEEECCCCCCCCHHHHHHHCCCC
CEDLKPVLVELLREHFGARPRQHPSAAQASVLVTR
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEC
>Mature Secondary Structure 
ANQFERLKSEKDGLAVKAELEAFARMGWENIPEDDRDHRLKWLGIFFRKRTPGQFMLRL
CCHHHHHHCCCCCCEEEHHHHHHHHCCCCCCCCCCHHCCEEEEEEEEECCCCCEEEEEE
RLPNGILTSGQMRMLGAIIHPYGEQGVADITTRQNLQLRGIPIEEMPQILGYLKEVGLTS
ECCCCCCCCCHHHHHHHHHCCCCCCCCEECCCCCCCEEECCCHHHHHHHHHHHHHHCHHH
IQSGMDNVRNITGSPLAGIDPDELIDVRGLTRKVQDMITNNGEGNPSFSNLPRKFNIAIC
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCEEEEEEE
GCRDNSVHAEINDLAFVPAFKNGRLGFNVLVGGFFSARRCAEAIGLDVWVDPRDVVPLCE
ECCCCCCEEEECCEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHH
AVLLVYRDHGLRANRQKARLMWLIDEWGLEKFRAAVERQTGHPLLRAAEKDEVVWHKRDL
HHHHHHHCCCCCCCCCCCEEEEEHHHCCHHHHHHHHHHCCCCHHHHCCCCCCEEEEHHHH
LGVHAQKQPGLNFVGLHVPVGRLNALEMMELARLAEVYGSGELRLTVEQNVLIPNVPDSR
HCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCEECCCCCCHH
VAPLLKEPLLKKFSPNPGPLQRGLVSCTGNQFCNFALIETKNRAVALMEELEAELEIPQT
HHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCE
VRIHWTGCPNSCGQPQVADIGLMGTTARKDGRVVEAVDIYMGGEVGKDAKLGECVRKGIP
EEEEEECCCCCCCCCCEEEEEEECCCCCCCCCEEEEEEEEECCCCCCCCHHHHHHHCCCC
CEDLKPVLVELLREHFGARPRQHPSAAQASVLVTR
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7766873 [H]