Click here to switch to the map view.

The map label for this gene is trmE

Identifier: 86606766

GI number: 86606766

Start: 2139799

End: 2141178

Strand: Reverse

Name: trmE

Synonym: CYA_2126

Alternate gene names: 86606766

Gene position: 2141178-2139799 (Counterclockwise)

Preceding gene: 86606772

Following gene: 86606765

Centisome position: 73.01

GC content: 66.88

Gene sequence:

>1380_bases
ATGGATCTGACTGCGCCCCTGGCCGATACCATTGCTGCCATTGCCACGGCAGTGGTGCCCGAACAGGGCAGCATTGGCAT
TGTACGCCTCTCCGGTTCGCGGGCTTTGGCCATTGCCCAGGCTATCTTTACTCCCGCCCGCCGCAACGCAGTCTGGGAAA
GCCATCGCCTCCTCTACGGCTGGATCCACGACGAGAAGGGCCGGCGGTTGGACGAGGCCCTGGCCGTCTGGATGCAGGCT
CCCCGCTCCTACACCCGCGAGGACGTAGTGGAGCTGCACTGCCACGGCGGCATCATGGTGGTTCAGGCCACGCTGCAGCA
GTGCCTGCGCCAGGGGGCACGGCTGGCGCAGCCGGGGGAGTTCAGCCTGCGCGCTTTCTTGAACGGCCGCATCGACCTCA
CCCAGGCCGAAAGCGTGGCCGATCTGGTGGCGGCCCGCTCCCCGCAGGCGGCCCAAATGGCCCTGGCGGGGCTGCAGGGC
AAGTTGGGGCAGTCCATCCGCGCCCTGCGCCAGGAGCTGCTGGGTTTATTGGCCGAGATCGAAGCCCGCCTCGACTTTGA
AGAGGATCTGCCCCCCCTGGACGTCCCCGCTTGGCAGGCCCGGCTGCAAGACATTCAGGCTCAGATGCAGGCCCTGCTGG
CCACCGCCGAGCGGGGGCAACTACTGCGCACTGGGGTGAAAGTGGCCATTGTCGGGCGGCCCAATGTGGGCAAGTCCAGC
CTGCTCAACGCCTGGTCGGGGCAGGATCGCGCTATTGTCACGGATCTACCTGGCACCACTCGGGATGTGGTGGAGTCCCA
GTTGGTGGTGAGAGGGATCCCCGTCCAGCTCCTGGATACCGCCGGCATTCGCGCCACCGACGATCCGGTGGAACGGCTAG
GGGTGGAGCGCTCCCAACGGCTGGCCCAGACCGCCGATGTGCTGGTGCTGGTCATCGACGCCCAGGCCGGCTGGACGGAG
GCTGATGCGGCTATCTATGCCTCCATCCGCCACCGCCCCCTCATCCTGGTGATCAACAAAACCGACCTCGCCCCCGCCGA
CAAGATCCGCCTCCCGCCCGAGATCGCCTACCGCGTCCCCGCCGTCGCCGCCCAAGGCCAAGGGATCCCGGAGCTGGAAG
AGGCCTTGGAGCAGCTTGTGACTCAGGGGCGGCCCCAGCCCAACCTGGAGGTGAGCCTCAACCAACGCCAGGCCGCCGCC
CTCCGCCAGGCCCAAGCCAGCCTGGAACAAGTCGTTCAGGCTATGCAGGCGCAGCTCCCCCTCGATTTTTGGACCATCGA
CCTGCGCGGCGCCCTGCACGCCCTTGGCCAAATCACCGGCGAGGAGATCTCCGAAGCGGTGCTGGATCAGATCTTCAGCC
GCTTCTGCATCGGCAAGTAA

Upstream 100 bases:

>100_bases
GGGAGGCGCGCCAATAGGCGCAAGAGACCTCTGCAGGGGATCGGGGAAAGCCGATGGGGAATCCAACAGTTGCGACAATT
CAAGCCGGGCTTTGTCGAGG

Downstream 100 bases:

>100_bases
GCGCTTGCTAACTCATACTCATTCCGAGTTTGTGTTAGTATGGACTCAGTCGGGAATCAGCAGGCCCCAGAGCCGAGGCG
GATCCCAGATAATGGAAACA

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MDLTAPLADTIAAIATAVVPEQGSIGIVRLSGSRALAIAQAIFTPARRNAVWESHRLLYGWIHDEKGRRLDEALAVWMQA
PRSYTREDVVELHCHGGIMVVQATLQQCLRQGARLAQPGEFSLRAFLNGRIDLTQAESVADLVAARSPQAAQMALAGLQG
KLGQSIRALRQELLGLLAEIEARLDFEEDLPPLDVPAWQARLQDIQAQMQALLATAERGQLLRTGVKVAIVGRPNVGKSS
LLNAWSGQDRAIVTDLPGTTRDVVESQLVVRGIPVQLLDTAGIRATDDPVERLGVERSQRLAQTADVLVLVIDAQAGWTE
ADAAIYASIRHRPLILVINKTDLAPADKIRLPPEIAYRVPAVAAQGQGIPELEEALEQLVTQGRPQPNLEVSLNQRQAAA
LRQAQASLEQVVQAMQAQLPLDFWTIDLRGALHALGQITGEEISEAVLDQIFSRFCIGK

Sequences:

>Translated_459_residues
MDLTAPLADTIAAIATAVVPEQGSIGIVRLSGSRALAIAQAIFTPARRNAVWESHRLLYGWIHDEKGRRLDEALAVWMQA
PRSYTREDVVELHCHGGIMVVQATLQQCLRQGARLAQPGEFSLRAFLNGRIDLTQAESVADLVAARSPQAAQMALAGLQG
KLGQSIRALRQELLGLLAEIEARLDFEEDLPPLDVPAWQARLQDIQAQMQALLATAERGQLLRTGVKVAIVGRPNVGKSS
LLNAWSGQDRAIVTDLPGTTRDVVESQLVVRGIPVQLLDTAGIRATDDPVERLGVERSQRLAQTADVLVLVIDAQAGWTE
ADAAIYASIRHRPLILVINKTDLAPADKIRLPPEIAYRVPAVAAQGQGIPELEEALEQLVTQGRPQPNLEVSLNQRQAAA
LRQAQASLEQVVQAMQAQLPLDFWTIDLRGALHALGQITGEEISEAVLDQIFSRFCIGK
>Mature_459_residues
MDLTAPLADTIAAIATAVVPEQGSIGIVRLSGSRALAIAQAIFTPARRNAVWESHRLLYGWIHDEKGRRLDEALAVWMQA
PRSYTREDVVELHCHGGIMVVQATLQQCLRQGARLAQPGEFSLRAFLNGRIDLTQAESVADLVAARSPQAAQMALAGLQG
KLGQSIRALRQELLGLLAEIEARLDFEEDLPPLDVPAWQARLQDIQAQMQALLATAERGQLLRTGVKVAIVGRPNVGKSS
LLNAWSGQDRAIVTDLPGTTRDVVESQLVVRGIPVQLLDTAGIRATDDPVERLGVERSQRLAQTADVLVLVIDAQAGWTE
ADAAIYASIRHRPLILVINKTDLAPADKIRLPPEIAYRVPAVAAQGQGIPELEEALEQLVTQGRPQPNLEVSLNQRQAAA
LRQAQASLEQVVQAMQAQLPLDFWTIDLRGALHALGQITGEEISEAVLDQIFSRFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=466, Percent_Identity=35.1931330472103, Blast_Score=201, Evalue=1e-51,
Organism=Homo sapiens, GI193082993, Length=455, Percent_Identity=35.6043956043956, Blast_Score=198, Evalue=1e-50,
Organism=Homo sapiens, GI193082988, Length=498, Percent_Identity=33.1325301204819, Blast_Score=191, Evalue=1e-48,
Organism=Escherichia coli, GI2367268, Length=468, Percent_Identity=36.7521367521368, Blast_Score=261, Evalue=6e-71,
Organism=Escherichia coli, GI87082120, Length=122, Percent_Identity=41.8032786885246, Blast_Score=91, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17507259, Length=462, Percent_Identity=29.4372294372294, Blast_Score=207, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6323665, Length=494, Percent_Identity=29.1497975708502, Blast_Score=212, Evalue=8e-56,
Organism=Drosophila melanogaster, GI45550813, Length=480, Percent_Identity=29.7916666666667, Blast_Score=182, Evalue=5e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_SYNJA (Q2JSU8)

Other databases:

- EMBL:   CP000239
- RefSeq:   YP_475529.1
- ProteinModelPortal:   Q2JSU8
- SMR:   Q2JSU8
- STRING:   Q2JSU8
- GeneID:   3899115
- GenomeReviews:   CP000239_GR
- KEGG:   cya:CYA_2126
- TIGR:   CYA_2126
- eggNOG:   COG0486
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- ProtClustDB:   PRK05291
- BioCyc:   SSP321327:CYA_2126-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- PRINTS:   PR00326
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 49846; Mature: 49846

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: NA

Important sites: BINDING 29-29 BINDING 91-91 BINDING 130-130 BINDING 459-459

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLTAPLADTIAAIATAVVPEQGSIGIVRLSGSRALAIAQAIFTPARRNAVWESHRLLYG
CCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHCHHHHHHHHHCCCEEEE
WIHDEKGRRLDEALAVWMQAPRSYTREDVVELHCHGGIMVVQATLQQCLRQGARLAQPGE
EEECCCCCHHHHHHHHHHHCCCCCCHHHHHEEEECCCEEHHHHHHHHHHHHCCCCCCCCC
FSLRAFLNGRIDLTQAESVADLVAARSPQAAQMALAGLQGKLGQSIRALRQELLGLLAEI
HHHHHHHCCCEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EARLDFEEDLPPLDVPAWQARLQDIQAQMQALLATAERGQLLRTGVKVAIVGRPNVGKSS
HHHCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEEECCCCCHHH
LLNAWSGQDRAIVTDLPGTTRDVVESQLVVRGIPVQLLDTAGIRATDDPVERLGVERSQR
HHHHCCCCCCEEEECCCCCHHHHHHHHHHHCCCCHHHHCCCCCCCCCCHHHHHCCHHHHH
LAQTADVLVLVIDAQAGWTEADAAIYASIRHRPLILVINKTDLAPADKIRLPPEIAYRVP
HHHHHCEEEEEEECCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHHCC
AVAAQGQGIPELEEALEQLVTQGRPQPNLEVSLNQRQAAALRQAQASLEQVVQAMQAQLP
CHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHCC
LDFWTIDLRGALHALGQITGEEISEAVLDQIFSRFCIGK
CEEEEEEHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDLTAPLADTIAAIATAVVPEQGSIGIVRLSGSRALAIAQAIFTPARRNAVWESHRLLYG
CCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHCHHHHHHHHHCCCEEEE
WIHDEKGRRLDEALAVWMQAPRSYTREDVVELHCHGGIMVVQATLQQCLRQGARLAQPGE
EEECCCCCHHHHHHHHHHHCCCCCCHHHHHEEEECCCEEHHHHHHHHHHHHCCCCCCCCC
FSLRAFLNGRIDLTQAESVADLVAARSPQAAQMALAGLQGKLGQSIRALRQELLGLLAEI
HHHHHHHCCCEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EARLDFEEDLPPLDVPAWQARLQDIQAQMQALLATAERGQLLRTGVKVAIVGRPNVGKSS
HHHCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEEECCCCCHHH
LLNAWSGQDRAIVTDLPGTTRDVVESQLVVRGIPVQLLDTAGIRATDDPVERLGVERSQR
HHHHCCCCCCEEEECCCCCHHHHHHHHHHHCCCCHHHHCCCCCCCCCCHHHHHCCHHHHH
LAQTADVLVLVIDAQAGWTEADAAIYASIRHRPLILVINKTDLAPADKIRLPPEIAYRVP
HHHHHCEEEEEEECCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHHCC
AVAAQGQGIPELEEALEQLVTQGRPQPNLEVSLNQRQAAALRQAQASLEQVVQAMQAQLP
CHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHCC
LDFWTIDLRGALHALGQITGEEISEAVLDQIFSRFCIGK
CEEEEEEHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA