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The map label for this gene is trmJ [H]

Identifier: 86606598

GI number: 86606598

Start: 1964218

End: 1965027

Strand: Reverse

Name: trmJ [H]

Synonym: CYA_1953

Alternate gene names: 86606598

Gene position: 1965027-1964218 (Counterclockwise)

Preceding gene: 86606603

Following gene: 86606597

Centisome position: 67.0

GC content: 63.09

Gene sequence:

>810_bases
ATGCCGGCATCGTCCAACTGGGCTCAGGAGCGATTGGCGCAGGTACGCCTTGTGTTAGTGCGGCCGGCAGGGCCGCGCAA
TCTCGGGGCCATTGCCCGCGTGATGAAAAACATGGGCCTGCGGCAGTGGGTGTTGGTGGATCCCCAATGCGATCCCCTCG
ACGCAGAAGCAGTCACCATGGCCGTGCATGCGACGGACTTGCTCCGCCAAGCCCGGCAGGTGCCAACCTTAGCCGAGGCC
CTTCAGGGATGTGTGCAGGTATTCGGGACAGCAGGCCGCCGCGAGCCCTACCCGCCCGAATGGCAAATTCAGTCTCCCCG
CCAGGCTTTCCCGGAGCTGCTGGCTGCCGGCCCTGCCGCTCTGGTGTTTGGCCCTGAAGATCGGGGCTTGAGCAACGAAG
AGCTGGCCCTCTGCCATCGCCAGATCCAGATCCCCACCGATCCGGCCTACCCTTCCCTGAACTTGGCCCAGGCAGTGGGC
ATCTGTTGCTACGAGCTGCGCACCCTCCTCTGCCAGAATCTTCCCCCGCCAGTGACCAGCTCTGGGATCTCAAAGCCGGC
TCCCTTCGAGCTGCTGGAGGGTTTTTTCGGCCACCTGGAGGCGCTGTTGCTGCAAATTGGCTACCTGCACCCCCACACCG
CTCGCCGCAAGATGCTCAAGCTGCGCGCCCTCTTTCACCGCGCCGGCCTGATCGTGTCCGAGGTGGCCCTGTTACGCGGG
ATCCTGCGACAACTCCAGTGGAGCCAAAAATCTTCTCCTCGAGAACCCCCAAAAAAAGAGGATCCTCATCCTGCTGAGAG
TGGATCCTAG

Upstream 100 bases:

>100_bases
TCCAACTCAGCCAACGGCGGTAGGCGGATCCCATCGGCGCAATCTATGACAAGATCTAACTCCTCTACTTTAGGGGATCC
CTATCTGCGATTTCTGAGCA

Downstream 100 bases:

>100_bases
CTGCCGCGGCCTCTGGCCGGCTGCCTCTGAAAACAAACCTCTGGGAACTGAGACAAGGGAATCCAGCTAGGTCTGGATGG
TCTTCCTGCGGCAAACTTGA

Product: RNA methyltransferase

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 269; Mature: 268

Protein sequence:

>269_residues
MPASSNWAQERLAQVRLVLVRPAGPRNLGAIARVMKNMGLRQWVLVDPQCDPLDAEAVTMAVHATDLLRQARQVPTLAEA
LQGCVQVFGTAGRREPYPPEWQIQSPRQAFPELLAAGPAALVFGPEDRGLSNEELALCHRQIQIPTDPAYPSLNLAQAVG
ICCYELRTLLCQNLPPPVTSSGISKPAPFELLEGFFGHLEALLLQIGYLHPHTARRKMLKLRALFHRAGLIVSEVALLRG
ILRQLQWSQKSSPREPPKKEDPHPAESGS

Sequences:

>Translated_269_residues
MPASSNWAQERLAQVRLVLVRPAGPRNLGAIARVMKNMGLRQWVLVDPQCDPLDAEAVTMAVHATDLLRQARQVPTLAEA
LQGCVQVFGTAGRREPYPPEWQIQSPRQAFPELLAAGPAALVFGPEDRGLSNEELALCHRQIQIPTDPAYPSLNLAQAVG
ICCYELRTLLCQNLPPPVTSSGISKPAPFELLEGFFGHLEALLLQIGYLHPHTARRKMLKLRALFHRAGLIVSEVALLRG
ILRQLQWSQKSSPREPPKKEDPHPAESGS
>Mature_268_residues
PASSNWAQERLAQVRLVLVRPAGPRNLGAIARVMKNMGLRQWVLVDPQCDPLDAEAVTMAVHATDLLRQARQVPTLAEAL
QGCVQVFGTAGRREPYPPEWQIQSPRQAFPELLAAGPAALVFGPEDRGLSNEELALCHRQIQIPTDPAYPSLNLAQAVGI
CCYELRTLLCQNLPPPVTSSGISKPAPFELLEGFFGHLEALLLQIGYLHPHTARRKMLKLRALFHRAGLIVSEVALLRGI
LRQLQWSQKSSPREPPKKEDPHPAESGS

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=248, Percent_Identity=39.1129032258064, Blast_Score=154, Evalue=8e-39,
Organism=Escherichia coli, GI1790865, Length=164, Percent_Identity=39.6341463414634, Blast_Score=95, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 29635; Mature: 29504

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPASSNWAQERLAQVRLVLVRPAGPRNLGAIARVMKNMGLRQWVLVDPQCDPLDAEAVTM
CCCCCHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHH
AVHATDLLRQARQVPTLAEALQGCVQVFGTAGRREPYPPEWQIQSPRQAFPELLAAGPAA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEE
LVFGPEDRGLSNEELALCHRQIQIPTDPAYPSLNLAQAVGICCYELRTLLCQNLPPPVTS
EEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
SGISKPAPFELLEGFFGHLEALLLQIGYLHPHTARRKMLKLRALFHRAGLIVSEVALLRG
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILRQLQWSQKSSPREPPKKEDPHPAESGS
HHHHHHHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PASSNWAQERLAQVRLVLVRPAGPRNLGAIARVMKNMGLRQWVLVDPQCDPLDAEAVTM
CCCCHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHH
AVHATDLLRQARQVPTLAEALQGCVQVFGTAGRREPYPPEWQIQSPRQAFPELLAAGPAA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEE
LVFGPEDRGLSNEELALCHRQIQIPTDPAYPSLNLAQAVGICCYELRTLLCQNLPPPVTS
EEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
SGISKPAPFELLEGFFGHLEALLLQIGYLHPHTARRKMLKLRALFHRAGLIVSEVALLRG
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILRQLQWSQKSSPREPPKKEDPHPAESGS
HHHHHHHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA