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The map label for this gene is potB

Identifier: 86606508

GI number: 86606508

Start: 1862898

End: 1863845

Strand: Reverse

Name: potB

Synonym: CYA_1858

Alternate gene names: 86606508

Gene position: 1863845-1862898 (Counterclockwise)

Preceding gene: 86606509

Following gene: 86606507

Centisome position: 63.55

GC content: 63.92

Gene sequence:

>948_bases
ATGCTGAAGCGGCCAGCAGCGGCAGAGCCAGGGATCCAGCGTTTCCAGGTGCGCTCAGGGGAGCTGCTCGCCCCTTGGCA
GGAGCTGCTGCGCAGCCTCATCTTTGCCGGGCCGGGGGTGCTCTGGGTGGTAACGTTTTTGGTGCTGCCGGGGCTGTTGC
TGCTGGTTTGCAGCTTTCTCAGCAGCGGCCAGTTTGGCCAGGTGGGCCTGCCCCTGACGTTGGCCAACTACCTGCGCATT
CTCGGCTATAGCCCCCTGGGCTGGACGCCCATCTACTGGCAGATCTTGGGCCGCAGCCTGCTGGTGGCGGCCGTCACCAC
CTTCTTCTGTGTGCTCCTGGCCTATCCCCTGGCTTTCTTCATTGCCGCCCACCCGCCCCGACAGCGGGATCTGTTGCTGA
CGCTGGTGGTGGTGCCCTTCTGGACCAACTTGGTGATCCGCACCTATGCCTGGATGGTGGTGCTGGCTCCGGAGGGGTGG
CCGGCACGGCTGGCGGCCTGGCTGGGCTGGATCCCGCCCCAGACAGCTCTCTACCCCAGTTGGCTGGCGGTGTACCTGGG
CATGGTCTACACCTTCTTGCCCTACATGGTCCTGCCCCTCTACACGGCCATAGAGCGCATCGACTGGCGCCTGGCAGAGG
CAGCCAGGGATCTCTACGCCAACGGCTGGCAGGTTTTCCTGTGGGTGCTTTTGCCCCAGACGCTGCCGGGGCTGGCGGCG
GGCCTGATCCTGGTGGCAATTCCGGCGCTGGGCATGTTTGTGGTGCCCGATTTGTTGGGGGGCAGCAAAACCTTGTTGAT
TGGCAATGTCATCCAGATGCAGTTTGGCGCCAGCCTGGACTACCCTTTTGGAGCGGCCCTCAGCTTTCTGGTTACCGGGC
TGAGCCTGGGGCTAATCTACGCCTATAGCCGCTACGTCGGCAGCCGCGGCCTGGAAGATCTGATCTAA

Upstream 100 bases:

>100_bases
TCGAGGATCAGGATTTTGCCATCGGGGAGGCCCTAGTGGCTCATCTCCCTCCCAAGAACTTGGTGCACTTGCAGCCGGAT
CCCCGGCGACAGGAGGCTCC

Downstream 100 bases:

>100_bases
GTCGCTGCAAACGGCTGGGATTGGATGGCGGATGCTGCCTGAAGCTTTGGTAGGTTGAGGATACTGGCGTTGCGCTAGAG
GGATCCCTTGACCCAAACGC

Product: spermidine/putrescine ABC transporter, permease protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MLKRPAAAEPGIQRFQVRSGELLAPWQELLRSLIFAGPGVLWVVTFLVLPGLLLLVCSFLSSGQFGQVGLPLTLANYLRI
LGYSPLGWTPIYWQILGRSLLVAAVTTFFCVLLAYPLAFFIAAHPPRQRDLLLTLVVVPFWTNLVIRTYAWMVVLAPEGW
PARLAAWLGWIPPQTALYPSWLAVYLGMVYTFLPYMVLPLYTAIERIDWRLAEAARDLYANGWQVFLWVLLPQTLPGLAA
GLILVAIPALGMFVVPDLLGGSKTLLIGNVIQMQFGASLDYPFGAALSFLVTGLSLGLIYAYSRYVGSRGLEDLI

Sequences:

>Translated_315_residues
MLKRPAAAEPGIQRFQVRSGELLAPWQELLRSLIFAGPGVLWVVTFLVLPGLLLLVCSFLSSGQFGQVGLPLTLANYLRI
LGYSPLGWTPIYWQILGRSLLVAAVTTFFCVLLAYPLAFFIAAHPPRQRDLLLTLVVVPFWTNLVIRTYAWMVVLAPEGW
PARLAAWLGWIPPQTALYPSWLAVYLGMVYTFLPYMVLPLYTAIERIDWRLAEAARDLYANGWQVFLWVLLPQTLPGLAA
GLILVAIPALGMFVVPDLLGGSKTLLIGNVIQMQFGASLDYPFGAALSFLVTGLSLGLIYAYSRYVGSRGLEDLI
>Mature_315_residues
MLKRPAAAEPGIQRFQVRSGELLAPWQELLRSLIFAGPGVLWVVTFLVLPGLLLLVCSFLSSGQFGQVGLPLTLANYLRI
LGYSPLGWTPIYWQILGRSLLVAAVTTFFCVLLAYPLAFFIAAHPPRQRDLLLTLVVVPFWTNLVIRTYAWMVVLAPEGW
PARLAAWLGWIPPQTALYPSWLAVYLGMVYTFLPYMVLPLYTAIERIDWRLAEAARDLYANGWQVFLWVLLPQTLPGLAA
GLILVAIPALGMFVVPDLLGGSKTLLIGNVIQMQFGASLDYPFGAALSFLVTGLSLGLIYAYSRYVGSRGLEDLI

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=273, Percent_Identity=43.5897435897436, Blast_Score=206, Evalue=1e-54,
Organism=Escherichia coli, GI1787080, Length=226, Percent_Identity=42.0353982300885, Blast_Score=174, Evalue=6e-45,
Organism=Escherichia coli, GI1787713, Length=249, Percent_Identity=32.9317269076305, Blast_Score=101, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 34843; Mature: 34843

Theoretical pI: Translated: 9.16; Mature: 9.16

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKRPAAAEPGIQRFQVRSGELLAPWQELLRSLIFAGPGVLWVVTFLVLPGLLLLVCSFL
CCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
SSGQFGQVGLPLTLANYLRILGYSPLGWTPIYWQILGRSLLVAAVTTFFCVLLAYPLAFF
CCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAAHPPRQRDLLLTLVVVPFWTNLVIRTYAWMVVLAPEGWPARLAAWLGWIPPQTALYPS
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHCCCCCCHHHHHH
WLAVYLGMVYTFLPYMVLPLYTAIERIDWRLAEAARDLYANGWQVFLWVLLPQTLPGLAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
GLILVAIPALGMFVVPDLLGGSKTLLIGNVIQMQFGASLDYPFGAALSFLVTGLSLGLIY
HHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
AYSRYVGSRGLEDLI
HHHHHHHCCCHHHCC
>Mature Secondary Structure
MLKRPAAAEPGIQRFQVRSGELLAPWQELLRSLIFAGPGVLWVVTFLVLPGLLLLVCSFL
CCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
SSGQFGQVGLPLTLANYLRILGYSPLGWTPIYWQILGRSLLVAAVTTFFCVLLAYPLAFF
CCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAAHPPRQRDLLLTLVVVPFWTNLVIRTYAWMVVLAPEGWPARLAAWLGWIPPQTALYPS
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHCCCCCCHHHHHH
WLAVYLGMVYTFLPYMVLPLYTAIERIDWRLAEAARDLYANGWQVFLWVLLPQTLPGLAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
GLILVAIPALGMFVVPDLLGGSKTLLIGNVIQMQFGASLDYPFGAALSFLVTGLSLGLIY
HHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
AYSRYVGSRGLEDLI
HHHHHHHCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]