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The map label for this gene is cbiG/cobJ-2

Identifier: 86606498

GI number: 86606498

Start: 1847074

End: 1848795

Strand: Reverse

Name: cbiG/cobJ-2

Synonym: CYA_1847

Alternate gene names: 86606498

Gene position: 1848795-1847074 (Counterclockwise)

Preceding gene: 86606502

Following gene: 86606495

Centisome position: 63.04

GC content: 64.58

Gene sequence:

>1722_bases
GTGCAGGGCTGTCTCTTCATCTTGGCCGCAGGGTCGGTGGTGCGGCTCATTGCCCCCCTCTTGCAGGACAAGCAGCGGGA
TCCAGCGGTGGTGGTGGTGGACGAAGCCGGTCGCTGGGCTATTAGCCTGTGCGGGGGACATGTGGGGGGGGCCGATGCCC
TGGCGCGGCAGGTGGCCGCTACCTTGGGAGCCCAGCCGGTTCTCACCTCTGCTTCCGAGGGGCTGGGGATCCCGCCTTTG
GATTTGCTGGGGGATCCCTACGGCTGGCGGCGGGGCGCTGGAGATTGGAACGGGGTGGCAGCCGCCTTGACGCGGGGAGA
GCCGGTGGCCGTTCACCAGACCTGCGGCACGCGGCTTTGGCGACAAGCTTGGCCGGCAGATCATCCCCTAGTGGCCTCCT
CTCCTCCCCATCCCGTTGCCCAGATCTGGATTGGGGAATACCTCTCCCCTCCCACGGGGATCCCGACAGTGCACTGGCAT
CCGCGGGTGCTCTGGGTGGGGGTGGGCTGCGAGCGGGGCACTACCCGCGTTTGGCTAGAGGGATCCCTGCGCCGCCTGTT
GCAGGCCCAGGGGCTGGCTTTTGAGGCCATTGCCGGCTTGGCCACCTTGGAGCTGAAGCAAAACGAGCCGGGTCTTCTGG
AGCTGGCCCAAGCCTATGGCTGGCCCATTGAGTTTTTCTCGGCAGAGGCTTTGGCTGGGATCCCAGTGCCCCACCCTTCG
GAGGCGGTGCGGCAGGCGGTGGGCACTCCCTCCGTAGCCGAGGCGGCTGCCCTCTTGGCAGCTCAGACTTGTCAGACTTG
GGGAAGAGGTGACAAGGTTCCTGAGAGGCCATCCTGTGTTGCGGAGAAGGTAGAGCCCTTTGGGGGAGCAACCCTCCTCT
GCCCCAAGCAGGTCTTCACCTCGAAAACCGAAGGGGCCTGTACCCTGGCCATTGCCCGCGCGGCGCAGGAGTATTCCCCT
CGCAGCGGCCACCTCTGGCTCATCGGCACTGGTCCTGGGGATCTGGGTCAATTGACTTTGGCTGCCCGTATGGCTTTAAC
TCAGGCCGATGTGGTTGTTGGCTACCAGCTTTATCTGGATCTGGTGGATCCCCTTTTGCACCCCGGCCAAATTCGGGAGG
CCTCTCCCATTACCCAAGAGCTGCAGCGGGCCAAACAGGCCATCGCTTGGGCGCAGCAGGGGCTGAAGGTGGCGGTGATT
TCTTCGGGGGATGCCGGCATTTACGGCATGGCGGGCTTGGTGCTGGAGTGCTTGGTCCGCTCTGGCTGGGATGGGATCCG
CCCTTCTCTGGAGGTGCTGCCGGGGATCTCTGCCTTCCAGGCGGCTGCTGCCCGGCTGGGGGCTCCGCTGATGCACGACT
TCTGCGCCATTTCCCTCTCGGATTTGCTTACCCCCTGGCCAGTGATTGCAAAACGCCTGCAGGCGGCTGCAGCTGCCGAT
TTTGTGGTGGCCCTTTACAACCCCCGCTCCCGGCAGCGGGTGGATCCCTTCCTCAAGGCTCTGCAGATCTTTTTGCAGGC
CCGGCCCGCCCATACCCCTGTGGCTTTGGCCCGCAACCTCTACCGCCCTGGCGAAACTCTCCGCCTTACCACCCTGGGGG
AGCTGGATCCAGAGCAGGTGGACATGCTCACCCTGGTTCTTATCGGCAACTCTCAGACCTTTCGCCATGGGGACCGGCTG
ATTACCCCGCGGGGCTATTGCCTGGGCGAAGAACCCACTTGA

Upstream 100 bases:

>100_bases
CCCGCAGGGGCTGCCCTCCCCCTGGCAGGCTTACGGGGATCCCAAGGATCCCACAGCGCCCAGATCTCTGGCAGAAGCCG
TTGCCCAAACCTGGCCCCAG

Downstream 100 bases:

>100_bases
CGGGTGTTAAGCCCAGACTCAAAACACCCTCAGATGCCGGGGATCCAACTGTAGCCACCAGGGGGATCCCCAAGTCTCCA
CCTCGCGCCAGCGCTCCCGC

Product: cbiG protein/precorrin-3B C17-methyltransferase

Products: S-adenosyl-L-homocysteine; Precorrin 4

Alternate protein names: Cobalt-precorrin-3 methylase; Cobalt-precorrin-3 methyltransferase [H]

Number of amino acids: Translated: 573; Mature: 573

Protein sequence:

>573_residues
MQGCLFILAAGSVVRLIAPLLQDKQRDPAVVVVDEAGRWAISLCGGHVGGADALARQVAATLGAQPVLTSASEGLGIPPL
DLLGDPYGWRRGAGDWNGVAAALTRGEPVAVHQTCGTRLWRQAWPADHPLVASSPPHPVAQIWIGEYLSPPTGIPTVHWH
PRVLWVGVGCERGTTRVWLEGSLRRLLQAQGLAFEAIAGLATLELKQNEPGLLELAQAYGWPIEFFSAEALAGIPVPHPS
EAVRQAVGTPSVAEAAALLAAQTCQTWGRGDKVPERPSCVAEKVEPFGGATLLCPKQVFTSKTEGACTLAIARAAQEYSP
RSGHLWLIGTGPGDLGQLTLAARMALTQADVVVGYQLYLDLVDPLLHPGQIREASPITQELQRAKQAIAWAQQGLKVAVI
SSGDAGIYGMAGLVLECLVRSGWDGIRPSLEVLPGISAFQAAAARLGAPLMHDFCAISLSDLLTPWPVIAKRLQAAAAAD
FVVALYNPRSRQRVDPFLKALQIFLQARPAHTPVALARNLYRPGETLRLTTLGELDPEQVDMLTLVLIGNSQTFRHGDRL
ITPRGYCLGEEPT

Sequences:

>Translated_573_residues
MQGCLFILAAGSVVRLIAPLLQDKQRDPAVVVVDEAGRWAISLCGGHVGGADALARQVAATLGAQPVLTSASEGLGIPPL
DLLGDPYGWRRGAGDWNGVAAALTRGEPVAVHQTCGTRLWRQAWPADHPLVASSPPHPVAQIWIGEYLSPPTGIPTVHWH
PRVLWVGVGCERGTTRVWLEGSLRRLLQAQGLAFEAIAGLATLELKQNEPGLLELAQAYGWPIEFFSAEALAGIPVPHPS
EAVRQAVGTPSVAEAAALLAAQTCQTWGRGDKVPERPSCVAEKVEPFGGATLLCPKQVFTSKTEGACTLAIARAAQEYSP
RSGHLWLIGTGPGDLGQLTLAARMALTQADVVVGYQLYLDLVDPLLHPGQIREASPITQELQRAKQAIAWAQQGLKVAVI
SSGDAGIYGMAGLVLECLVRSGWDGIRPSLEVLPGISAFQAAAARLGAPLMHDFCAISLSDLLTPWPVIAKRLQAAAAAD
FVVALYNPRSRQRVDPFLKALQIFLQARPAHTPVALARNLYRPGETLRLTTLGELDPEQVDMLTLVLIGNSQTFRHGDRL
ITPRGYCLGEEPT
>Mature_573_residues
MQGCLFILAAGSVVRLIAPLLQDKQRDPAVVVVDEAGRWAISLCGGHVGGADALARQVAATLGAQPVLTSASEGLGIPPL
DLLGDPYGWRRGAGDWNGVAAALTRGEPVAVHQTCGTRLWRQAWPADHPLVASSPPHPVAQIWIGEYLSPPTGIPTVHWH
PRVLWVGVGCERGTTRVWLEGSLRRLLQAQGLAFEAIAGLATLELKQNEPGLLELAQAYGWPIEFFSAEALAGIPVPHPS
EAVRQAVGTPSVAEAAALLAAQTCQTWGRGDKVPERPSCVAEKVEPFGGATLLCPKQVFTSKTEGACTLAIARAAQEYSP
RSGHLWLIGTGPGDLGQLTLAARMALTQADVVVGYQLYLDLVDPLLHPGQIREASPITQELQRAKQAIAWAQQGLKVAVI
SSGDAGIYGMAGLVLECLVRSGWDGIRPSLEVLPGISAFQAAAARLGAPLMHDFCAISLSDLLTPWPVIAKRLQAAAAAD
FVVALYNPRSRQRVDPFLKALQIFLQARPAHTPVALARNLYRPGETLRLTTLGELDPEQVDMLTLVLIGNSQTFRHGDRL
ITPRGYCLGEEPT

Specific function: Methyltransferase that catalyzes the methylation of C-17 in cobalt-precorrin-3B to form cobalt-precorrin-4 [H]

COG id: COG1010

COG function: function code H; Precorrin-3B methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006363 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.131

Molecular weight: Translated: 61071; Mature: 61071

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQGCLFILAAGSVVRLIAPLLQDKQRDPAVVVVDEAGRWAISLCGGHVGGADALARQVAA
CCCEEEEEEHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEECCCCCCCHHHHHHHHHH
TLGAQPVLTSASEGLGIPPLDLLGDPYGWRRGAGDWNGVAAALTRGEPVAVHQTCGTRLW
HHCCCCHHCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCHHHHCCCCCCEEEHHHHHHHHH
RQAWPADHPLVASSPPHPVAQIWIGEYLSPPTGIPTVHWHPRVLWVGVGCERGTTRVWLE
HHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCEEEECCEEEEEEECCCCCCEEEEEC
GSLRRLLQAQGLAFEAIAGLATLELKQNEPGLLELAQAYGWPIEFFSAEALAGIPVPHPS
HHHHHHHHHCCCHHHHHHCEEEEEECCCCCCHHHHHHHCCCCCEECCCHHHCCCCCCCCH
EAVRQAVGTPSVAEAAALLAAQTCQTWGRGDKVPERPSCVAEKVEPFGGATLLCPKQVFT
HHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCEEEEECHHHHC
SKTEGACTLAIARAAQEYSPRSGHLWLIGTGPGDLGQLTLAARMALTQADVVVGYQLYLD
CCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LVDPLLHPGQIREASPITQELQRAKQAIAWAQQGLKVAVISSGDAGIYGMAGLVLECLVR
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHH
SGWDGIRPSLEVLPGISAFQAAAARLGAPLMHDFCAISLSDLLTPWPVIAKRLQAAAAAD
CCCCCCCCHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHC
FVVALYNPRSRQRVDPFLKALQIFLQARPAHTPVALARNLYRPGETLRLTTLGELDPEQV
EEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEEEEECCCCHHHH
DMLTLVLIGNSQTFRHGDRLITPRGYCLGEEPT
CEEEEEEECCCCCCCCCCEEECCCCCCCCCCCC
>Mature Secondary Structure
MQGCLFILAAGSVVRLIAPLLQDKQRDPAVVVVDEAGRWAISLCGGHVGGADALARQVAA
CCCEEEEEEHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEECCCCCCCHHHHHHHHHH
TLGAQPVLTSASEGLGIPPLDLLGDPYGWRRGAGDWNGVAAALTRGEPVAVHQTCGTRLW
HHCCCCHHCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCHHHHCCCCCCEEEHHHHHHHHH
RQAWPADHPLVASSPPHPVAQIWIGEYLSPPTGIPTVHWHPRVLWVGVGCERGTTRVWLE
HHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCEEEECCEEEEEEECCCCCCEEEEEC
GSLRRLLQAQGLAFEAIAGLATLELKQNEPGLLELAQAYGWPIEFFSAEALAGIPVPHPS
HHHHHHHHHCCCHHHHHHCEEEEEECCCCCCHHHHHHHCCCCCEECCCHHHCCCCCCCCH
EAVRQAVGTPSVAEAAALLAAQTCQTWGRGDKVPERPSCVAEKVEPFGGATLLCPKQVFT
HHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCEEEEECHHHHC
SKTEGACTLAIARAAQEYSPRSGHLWLIGTGPGDLGQLTLAARMALTQADVVVGYQLYLD
CCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LVDPLLHPGQIREASPITQELQRAKQAIAWAQQGLKVAVISSGDAGIYGMAGLVLECLVR
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHH
SGWDGIRPSLEVLPGISAFQAAAARLGAPLMHDFCAISLSDLLTPWPVIAKRLQAAAAAD
CCCCCCCCHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHC
FVVALYNPRSRQRVDPFLKALQIFLQARPAHTPVALARNLYRPGETLRLTTLGELDPEQV
EEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEEEEECCCCHHHH
DMLTLVLIGNSQTFRHGDRLITPRGYCLGEEPT
CEEEEEEECCCCCCCCCCEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 3B

Specific reaction: S-adenosyl-L-methionine + precorrin-3B = S-adenosyl-L-homocysteine + precorrin-4

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8501034; 11677609 [H]