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The map label for this gene is nifD

Identifier: 86606474

GI number: 86606474

Start: 1827528

End: 1828991

Strand: Reverse

Name: nifD

Synonym: CYA_1823

Alternate gene names: 86606474

Gene position: 1828991-1827528 (Counterclockwise)

Preceding gene: 86606475

Following gene: 86606473

Centisome position: 62.36

GC content: 58.33

Gene sequence:

>1464_bases
ATGACTACTCCAAAAAGCGCCCTCAAAGAGGAAAACCGAAAAGTTATTGCAGAAGTTCTGGAAACCTATCCGGAAAAGGC
CAAGCAAAAGCGAGCCAAACACCTCAACGTCTATGACGAGGGCAAAACGGATTGCGGAGTAAAGTCCAACATCAAGTCGA
TCCCCGGCGTCATGACCACTCGCGGCTGTGCCTATGCCGGTTCCAAGGGGGTGGTGTGGGGGCCGGTCAAAGACATGATC
CACCTCAGCCATGGCCCCGTGGGCTGCGGCTACTACTCCTGGTCGGGAAGGCGCAATTACTACGTCGGCACAACCGGGGT
AGATACCTTCGTTACCATGCAGTTCACCTCCGATTTTCAGGAGCGAGATGTGGTGTTCGGGGGGGACAAAAAACTGGCCA
AACTCTTGGTGGAAGCCAGCGAACTCTTCCCCTTGGCCAAAGGCATTACCATCCAGTCCGAATGCCCCGTCGGCTTGATC
GGGGATGACATCGAGGCAGTGGCCAAGAGAGTTTCCAAAGAAATTGGCAAGCCGGTGGTACCGGTGCGCTGCGAAGGCTT
TCGGGGGGTCTCCCAGTCCCTGGGCCACCACATCGCCAACGATACGGTGCGGGACTGGGTTTTGCCCATTGCTGACAAGA
AAGCGAAGGAGGGATCCCTCAACTTTGAGCCCGGCCCCTACGATGTGGCCCTGATTGGGGACTACAACATCGGCGGCGAC
GCTTGGTCTTCCCGGATTTTGCTGGAGGAGATGGGCCTGCGGGTAGTGACCCAGTTTTCCGGCGATGGCACCTGGAACGA
GATGCTGCTTGCCCCTCGGGTGAAGCTGAATCTCATCCACTGCTACCGCTCCATGAACTACATCTGCCGCCACATGGAGG
AAGCCTACGGGATCCCCTGGCTGGAGTTCAACTTCTTTGGCCCCACCCAAATTGCCAAGTCGCTGCGGGCCATCGCCCAG
CGGTTCGACGAGACCATTCAGGCCAAGGCGGAGCAGGTGATCGCCAAGTACCAGCCCCAGAGCGAGGCTGTCATTGCCAA
GTATCGTCCCCGCCTCGAGGGCAAACGGGTGATGCTGATGGTGGGCGGACTGCGACCCCGCCACGTCATCCCGGCCTTCC
GGGAGCTGGGCATGGAGGTGATTGCCACCGGCTACGAATTCGGCCACGGCGACGACTACAAGCGCACCACCCAGTACATC
GACGACGGCACCCTCATCTACGATGACGTGTCCGGCTACGAGTTTGAAGAATTTGCCAAGAGGTTGAAGCCAGATCTCAT
CGCCTCCGGCATCAAGGAGAAGTACGTCTTTCAAAAGATGGCTTTGCCCTTCCGCCAGATGCACTCTTGGGACTACTCCG
GCCCCTACCACGGCTACGACGGCTTTGCCGTGTTCGCCCGCGATATGGATCTGGCCTTGAATAGCCCCACCTGGGATCTG
GTCAAAGCCCCCTGGAAGTCTTAG

Upstream 100 bases:

>100_bases
GTGAACTTAAAGATGGGCTGGGGGTGGATCCCTTCCTCCCTCGGCCCTCGGTTCCCCCTAGTTTCTTTCTCAAAAAAATC
CAGTAGATGAGGCTCTTCCC

Downstream 100 bases:

>100_bases
CTTGCCGCCGGGATCCCAGACGCCAACGGCCCTTCCTTCCCCTGCCCCTCATTAGGAGACCGACCATGCCGCAAGATATC
CAAGACATCAAAGATCACGC

Product: nitrogenase molybdenum-iron protein alpha chain

Products: NA

Alternate protein names: Dinitrogenase; Nitrogenase component I [H]

Number of amino acids: Translated: 487; Mature: 486

Protein sequence:

>487_residues
MTTPKSALKEENRKVIAEVLETYPEKAKQKRAKHLNVYDEGKTDCGVKSNIKSIPGVMTTRGCAYAGSKGVVWGPVKDMI
HLSHGPVGCGYYSWSGRRNYYVGTTGVDTFVTMQFTSDFQERDVVFGGDKKLAKLLVEASELFPLAKGITIQSECPVGLI
GDDIEAVAKRVSKEIGKPVVPVRCEGFRGVSQSLGHHIANDTVRDWVLPIADKKAKEGSLNFEPGPYDVALIGDYNIGGD
AWSSRILLEEMGLRVVTQFSGDGTWNEMLLAPRVKLNLIHCYRSMNYICRHMEEAYGIPWLEFNFFGPTQIAKSLRAIAQ
RFDETIQAKAEQVIAKYQPQSEAVIAKYRPRLEGKRVMLMVGGLRPRHVIPAFRELGMEVIATGYEFGHGDDYKRTTQYI
DDGTLIYDDVSGYEFEEFAKRLKPDLIASGIKEKYVFQKMALPFRQMHSWDYSGPYHGYDGFAVFARDMDLALNSPTWDL
VKAPWKS

Sequences:

>Translated_487_residues
MTTPKSALKEENRKVIAEVLETYPEKAKQKRAKHLNVYDEGKTDCGVKSNIKSIPGVMTTRGCAYAGSKGVVWGPVKDMI
HLSHGPVGCGYYSWSGRRNYYVGTTGVDTFVTMQFTSDFQERDVVFGGDKKLAKLLVEASELFPLAKGITIQSECPVGLI
GDDIEAVAKRVSKEIGKPVVPVRCEGFRGVSQSLGHHIANDTVRDWVLPIADKKAKEGSLNFEPGPYDVALIGDYNIGGD
AWSSRILLEEMGLRVVTQFSGDGTWNEMLLAPRVKLNLIHCYRSMNYICRHMEEAYGIPWLEFNFFGPTQIAKSLRAIAQ
RFDETIQAKAEQVIAKYQPQSEAVIAKYRPRLEGKRVMLMVGGLRPRHVIPAFRELGMEVIATGYEFGHGDDYKRTTQYI
DDGTLIYDDVSGYEFEEFAKRLKPDLIASGIKEKYVFQKMALPFRQMHSWDYSGPYHGYDGFAVFARDMDLALNSPTWDL
VKAPWKS
>Mature_486_residues
TTPKSALKEENRKVIAEVLETYPEKAKQKRAKHLNVYDEGKTDCGVKSNIKSIPGVMTTRGCAYAGSKGVVWGPVKDMIH
LSHGPVGCGYYSWSGRRNYYVGTTGVDTFVTMQFTSDFQERDVVFGGDKKLAKLLVEASELFPLAKGITIQSECPVGLIG
DDIEAVAKRVSKEIGKPVVPVRCEGFRGVSQSLGHHIANDTVRDWVLPIADKKAKEGSLNFEPGPYDVALIGDYNIGGDA
WSSRILLEEMGLRVVTQFSGDGTWNEMLLAPRVKLNLIHCYRSMNYICRHMEEAYGIPWLEFNFFGPTQIAKSLRAIAQR
FDETIQAKAEQVIAKYQPQSEAVIAKYRPRLEGKRVMLMVGGLRPRHVIPAFRELGMEVIATGYEFGHGDDYKRTTQYID
DGTLIYDDVSGYEFEEFAKRLKPDLIASGIKEKYVFQKMALPFRQMHSWDYSGPYHGYDGFAVFARDMDLALNSPTWDLV
KAPWKS

Specific function: This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR010143
- InterPro:   IPR000318
- InterPro:   IPR005972 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 54707; Mature: 54576

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: PS00699 NITROGENASE_1_1 ; PS00090 NITROGENASE_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPKSALKEENRKVIAEVLETYPEKAKQKRAKHLNVYDEGKTDCGVKSNIKSIPGVMTT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHCCCCHHC
RGCAYAGSKGVVWGPVKDMIHLSHGPVGCGYYSWSGRRNYYVGTTGVDTFVTMQFTSDFQ
CCCCCCCCCCCEECCHHHHHHHCCCCCCCCEECCCCCCEEEEECCCCCEEEEEEECCCCC
ERDVVFGGDKKLAKLLVEASELFPLAKGITIQSECPVGLIGDDIEAVAKRVSKEIGKPVV
CCCEEECCCHHHHHHHHHHHHHHHHHCCCEEECCCCEEECCCCHHHHHHHHHHHHCCCCC
PVRCEGFRGVSQSLGHHIANDTVRDWVLPIADKKAKEGSLNFEPGPYDVALIGDYNIGGD
EEEECCCCCHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCH
AWSSRILLEEMGLRVVTQFSGDGTWNEMLLAPRVKLNLIHCYRSMNYICRHMEEAYGIPW
HHHHHHHHHHCCCEEEEEECCCCCHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCE
LEFNFFGPTQIAKSLRAIAQRFDETIQAKAEQVIAKYQPQSEAVIAKYRPRLEGKRVMLM
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCEEEEE
VGGLRPRHVIPAFRELGMEVIATGYEFGHGDDYKRTTQYIDDGTLIYDDVSGYEFEEFAK
ECCCCCCHHHHHHHHCCHHHHHCCCCCCCCCCHHHHHHHCCCCEEEEECCCCCCHHHHHH
RLKPDLIASGIKEKYVFQKMALPFRQMHSWDYSGPYHGYDGFAVFARDMDLALNSPTWDL
HCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCEEEEEECHHHEECCCCHHH
VKAPWKS
HCCCCCC
>Mature Secondary Structure 
TTPKSALKEENRKVIAEVLETYPEKAKQKRAKHLNVYDEGKTDCGVKSNIKSIPGVMTT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHCCCCHHC
RGCAYAGSKGVVWGPVKDMIHLSHGPVGCGYYSWSGRRNYYVGTTGVDTFVTMQFTSDFQ
CCCCCCCCCCCEECCHHHHHHHCCCCCCCCEECCCCCCEEEEECCCCCEEEEEEECCCCC
ERDVVFGGDKKLAKLLVEASELFPLAKGITIQSECPVGLIGDDIEAVAKRVSKEIGKPVV
CCCEEECCCHHHHHHHHHHHHHHHHHCCCEEECCCCEEECCCCHHHHHHHHHHHHCCCCC
PVRCEGFRGVSQSLGHHIANDTVRDWVLPIADKKAKEGSLNFEPGPYDVALIGDYNIGGD
EEEECCCCCHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCH
AWSSRILLEEMGLRVVTQFSGDGTWNEMLLAPRVKLNLIHCYRSMNYICRHMEEAYGIPW
HHHHHHHHHHCCCEEEEEECCCCCHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCE
LEFNFFGPTQIAKSLRAIAQRFDETIQAKAEQVIAKYQPQSEAVIAKYRPRLEGKRVMLM
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCEEEEE
VGGLRPRHVIPAFRELGMEVIATGYEFGHGDDYKRTTQYIDDGTLIYDDVSGYEFEEFAK
ECCCCCCHHHHHHHHCCHHHHHCCCCCCCCCCHHHHHHHCCCCEEEEECCCCCCHHHHHH
RLKPDLIASGIKEKYVFQKMALPFRQMHSWDYSGPYHGYDGFAVFARDMDLALNSPTWDL
HCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCEEEEEECHHHEECCCCHHH
VKAPWKS
HCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA