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The map label for this gene is nifK

Identifier: 86606473

GI number: 86606473

Start: 1825927

End: 1827462

Strand: Reverse

Name: nifK

Synonym: CYA_1821

Alternate gene names: 86606473

Gene position: 1827462-1825927 (Counterclockwise)

Preceding gene: 86606474

Following gene: 86606471

Centisome position: 62.31

GC content: 58.79

Gene sequence:

>1536_bases
ATGCCGCAAGATATCCAAGACATCAAAGATCACGCCCGCCTGTTTCAAGATCCGGAATATCAGGCGCTTCTGCAGTGCAA
GCAAACCTTTGAAGATCTCCCTTCCCCGGAAGAAGTGGCCCGGGTGGCCGAATGGACAAAATCTTGGGAATACCGGGAGA
AAAACTTTCAGCGGGAAGCTTTGACGATAAACCCTGCCAAGGCTTGCCAGCCCTTAGGGGCCATCTTCGCCGCTGTCGGC
TTCGAGGGAACTTTGCCCTTCGTCCATGGATCCCAGGGGTGTGTGGCCTATTTTCGCACCCATCTCACCCGCCACTTTAA
GGAGCCCTTTTCGGCGGTCTCCACCTCGATGACGGAAGATGCGGCGGTGTTTGGCGGCCTCAAAAACCTAATCGAGGGAC
TGGAAAATGCCTACGCCCTCTACAAGCCCAAGATGATCGCCGTGTGCACCACCTGCATGGCGGAGGTGATCGGGGATGAC
CTGGGGGCCTTCATCACCACGGCCAGAAATGAGGGAGTGATTTCCCGCGACTTCCCGGTGCCCTATGCCCACACGCCAAG
CTTTGTGGGATCCCACATCACCGGCTACGACAACATGCTCAAGGCCATTCTCAGCACCCTCACCGAGCCGGTGGGCCGCA
CGGGTAGCAATGGCTACATCAACGTCATCCCTGGCTTCGATACCTACATGGGCAACTTGCGGGAGATCAAGCGCATCTTC
GGCTTATTCGGGATCCCGCTGACCCTGCTGGCGGACAACTCCGACTATTTGGATTCGCCCAACGACGGCACTTTCCACAT
GTATCCCGGAGGAACCCCTTTGGAGGCGGCTGCCCAATGCCTCAACGCCGAGGCTACTTTTTCCTTCCAGCGCTACACCA
CGCCCAAGACCCTGGAGTTTCTAGCAGAGGCCTCGGGTCGGCCCACCCTGACGGCGCGGCCAGTGGGTATTCGGGGCACG
GATGAGTTTTTGATGCAGCTATCCCAGTTAACGGGCAAGCCCATTCCCGCCGAGCTGGAGATCGAACGGGGCCGGGCGGT
GGATGCTTTCACGGACTCCCAAGCGTGGATCCACGGCAAGCGGGTAGCCCTGTATGGGGATCCGGATCTGGTGATGGGCT
TGCTCAGCCTGTTGCTGGAGCTGGGAGCGGAGCCGATCCATGTGGTGGTGACCAACAGCAACGAGGAGTTCGAAGCCGAG
GCGCGGGCACTGCTGGCCTCCAGTCCCTATGGACAGGGGGCAACCGTCTGGGGGGGCAAGGATCTCTGGCATCTGCGCTC
TCTGATGTTCACCGAGCCGGTGGATCTGCTGATCGGCAATTCCTACGGCAAATACCTGTGGCGGGATACGGGCACGCCTT
TGGTGCGCATCGGCTACCCCATCTTCGACCGCCACCATCTGCATCGCTATCCCACCTATGGCTATGTGGGGGCGCTCAAC
CTCCTTAACTGGATTGTCAACACCATTCTGGATGAGGCAGATCGGCGCAGCATCATCCCCGGCAAAACCGATTTTTCCTT
CGACTTAATTCGTTAG

Upstream 100 bases:

>100_bases
CCTGGGATCTGGTCAAAGCCCCCTGGAAGTCTTAGCTTGCCGCCGGGATCCCAGACGCCAACGGCCCTTCCTTCCCCTGC
CCCTCATTAGGAGACCGACC

Downstream 100 bases:

>100_bases
CTTCCGGAGTAGGGGGATCCCAGAGGCTAGATAGATCTTGTCAAGGATCCCCTGCTCCAAAGACTCCACCCCGCTAAGAG
GAACAGCCAAGCCTTACAGG

Product: nitrogenase molybdenum-iron protein beta chain

Products: NA

Alternate protein names: Dinitrogenase; Nitrogenase component I [H]

Number of amino acids: Translated: 511; Mature: 510

Protein sequence:

>511_residues
MPQDIQDIKDHARLFQDPEYQALLQCKQTFEDLPSPEEVARVAEWTKSWEYREKNFQREALTINPAKACQPLGAIFAAVG
FEGTLPFVHGSQGCVAYFRTHLTRHFKEPFSAVSTSMTEDAAVFGGLKNLIEGLENAYALYKPKMIAVCTTCMAEVIGDD
LGAFITTARNEGVISRDFPVPYAHTPSFVGSHITGYDNMLKAILSTLTEPVGRTGSNGYINVIPGFDTYMGNLREIKRIF
GLFGIPLTLLADNSDYLDSPNDGTFHMYPGGTPLEAAAQCLNAEATFSFQRYTTPKTLEFLAEASGRPTLTARPVGIRGT
DEFLMQLSQLTGKPIPAELEIERGRAVDAFTDSQAWIHGKRVALYGDPDLVMGLLSLLLELGAEPIHVVVTNSNEEFEAE
ARALLASSPYGQGATVWGGKDLWHLRSLMFTEPVDLLIGNSYGKYLWRDTGTPLVRIGYPIFDRHHLHRYPTYGYVGALN
LLNWIVNTILDEADRRSIIPGKTDFSFDLIR

Sequences:

>Translated_511_residues
MPQDIQDIKDHARLFQDPEYQALLQCKQTFEDLPSPEEVARVAEWTKSWEYREKNFQREALTINPAKACQPLGAIFAAVG
FEGTLPFVHGSQGCVAYFRTHLTRHFKEPFSAVSTSMTEDAAVFGGLKNLIEGLENAYALYKPKMIAVCTTCMAEVIGDD
LGAFITTARNEGVISRDFPVPYAHTPSFVGSHITGYDNMLKAILSTLTEPVGRTGSNGYINVIPGFDTYMGNLREIKRIF
GLFGIPLTLLADNSDYLDSPNDGTFHMYPGGTPLEAAAQCLNAEATFSFQRYTTPKTLEFLAEASGRPTLTARPVGIRGT
DEFLMQLSQLTGKPIPAELEIERGRAVDAFTDSQAWIHGKRVALYGDPDLVMGLLSLLLELGAEPIHVVVTNSNEEFEAE
ARALLASSPYGQGATVWGGKDLWHLRSLMFTEPVDLLIGNSYGKYLWRDTGTPLVRIGYPIFDRHHLHRYPTYGYVGALN
LLNWIVNTILDEADRRSIIPGKTDFSFDLIR
>Mature_510_residues
PQDIQDIKDHARLFQDPEYQALLQCKQTFEDLPSPEEVARVAEWTKSWEYREKNFQREALTINPAKACQPLGAIFAAVGF
EGTLPFVHGSQGCVAYFRTHLTRHFKEPFSAVSTSMTEDAAVFGGLKNLIEGLENAYALYKPKMIAVCTTCMAEVIGDDL
GAFITTARNEGVISRDFPVPYAHTPSFVGSHITGYDNMLKAILSTLTEPVGRTGSNGYINVIPGFDTYMGNLREIKRIFG
LFGIPLTLLADNSDYLDSPNDGTFHMYPGGTPLEAAAQCLNAEATFSFQRYTTPKTLEFLAEASGRPTLTARPVGIRGTD
EFLMQLSQLTGKPIPAELEIERGRAVDAFTDSQAWIHGKRVALYGDPDLVMGLLSLLLELGAEPIHVVVTNSNEEFEAEA
RALLASSPYGQGATVWGGKDLWHLRSLMFTEPVDLLIGNSYGKYLWRDTGTPLVRIGYPIFDRHHLHRYPTYGYVGALNL
LNWIVNTILDEADRRSIIPGKTDFSFDLIR

Specific function: This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005976 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 56708; Mature: 56577

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00039 DEAD_ATP_HELICASE ; PS00699 NITROGENASE_1_1 ; PS00090 NITROGENASE_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQDIQDIKDHARLFQDPEYQALLQCKQTFEDLPSPEEVARVAEWTKSWEYREKNFQREA
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCE
LTINPAKACQPLGAIFAAVGFEGTLPFVHGSQGCVAYFRTHLTRHFKEPFSAVSTSMTED
EEECHHHHHHHHHHHHHHHCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAVFGGLKNLIEGLENAYALYKPKMIAVCTTCMAEVIGDDLGAFITTARNEGVISRDFPV
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCC
PYAHTPSFVGSHITGYDNMLKAILSTLTEPVGRTGSNGYINVIPGFDTYMGNLREIKRIF
CCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHCCHHHHHHHH
GLFGIPLTLLADNSDYLDSPNDGTFHMYPGGTPLEAAAQCLNAEATFSFQRYTTPKTLEF
HHHCCCEEEEECCCCCCCCCCCCEEEECCCCCCHHHHHHHHCCCCEEEEHHCCCHHHHHH
LAEASGRPTLTARPVGIRGTDEFLMQLSQLTGKPIPAELEIERGRAVDAFTDSQAWIHGK
HHHCCCCCCEEECCCCCCCHHHHHHHHHHHCCCCCCCCEEECCCCEEECCCCCCCEEECC
RVALYGDPDLVMGLLSLLLELGAEPIHVVVTNSNEEFEAEARALLASSPYGQGATVWGGK
EEEEECCHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCEEECCC
DLWHLRSLMFTEPVDLLIGNSYGKYLWRDTGTPLVRIGYPIFDRHHLHRYPTYGYVGALN
HHHHHHHHHHCCCHHHEECCCCCCEEECCCCCCEEEECCCHHCCCHHHCCCCCHHHHHHH
LLNWIVNTILDEADRRSIIPGKTDFSFDLIR
HHHHHHHHHHHHHCCCCCCCCCCCCCEECCC
>Mature Secondary Structure 
PQDIQDIKDHARLFQDPEYQALLQCKQTFEDLPSPEEVARVAEWTKSWEYREKNFQREA
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCE
LTINPAKACQPLGAIFAAVGFEGTLPFVHGSQGCVAYFRTHLTRHFKEPFSAVSTSMTED
EEECHHHHHHHHHHHHHHHCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAVFGGLKNLIEGLENAYALYKPKMIAVCTTCMAEVIGDDLGAFITTARNEGVISRDFPV
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCC
PYAHTPSFVGSHITGYDNMLKAILSTLTEPVGRTGSNGYINVIPGFDTYMGNLREIKRIF
CCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHCCHHHHHHHH
GLFGIPLTLLADNSDYLDSPNDGTFHMYPGGTPLEAAAQCLNAEATFSFQRYTTPKTLEF
HHHCCCEEEEECCCCCCCCCCCCEEEECCCCCCHHHHHHHHCCCCEEEEHHCCCHHHHHH
LAEASGRPTLTARPVGIRGTDEFLMQLSQLTGKPIPAELEIERGRAVDAFTDSQAWIHGK
HHHCCCCCCEEECCCCCCCHHHHHHHHHHHCCCCCCCCEEECCCCEEECCCCCCCEEECC
RVALYGDPDLVMGLLSLLLELGAEPIHVVVTNSNEEFEAEARALLASSPYGQGATVWGGK
EEEEECCHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCEEECCC
DLWHLRSLMFTEPVDLLIGNSYGKYLWRDTGTPLVRIGYPIFDRHHLHRYPTYGYVGALN
HHHHHHHHHHCCCHHHEECCCCCCEEECCCCCCEEEECCCHHCCCHHHCCCCCHHHHHHH
LLNWIVNTILDEADRRSIIPGKTDFSFDLIR
HHHHHHHHHHHHHCCCCCCCCCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA