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The map label for this gene is ntcA

Identifier: 86606452

GI number: 86606452

Start: 1799868

End: 1800542

Strand: Reverse

Name: ntcA

Synonym: CYA_1799

Alternate gene names: 86606452

Gene position: 1800542-1799868 (Counterclockwise)

Preceding gene: 86606455

Following gene: 86606448

Centisome position: 61.39

GC content: 56.74

Gene sequence:

>675_bases
ATGGCAGTGGCCATTGCAGAAAAATCTCTGGTTGCAGCCTTCAAACAGTTGGGAGGGCCTCACCATCCTCCGGTTATCGA
AACGTTTGAGCGCGGTAAGACCATCTTCTTTCCTGGGGATCCGGCGGAGCGGGTTTACTTTTTGGTGAAAGGAGCTGTCA
AGCTCTCGCGGGTCTACGAAATGGGGGAAGAAATTACCGTCGCTCTCCTGCGGGAAAACAGCATCTTTGGGGTGCTCTCC
TTCATCACGGGGCAGCGCTCCGACCGCTTCTATCACGCGGTGGCCTTTACCCGCGTCGAGCTGCTCTCTCTGCCCATCGA
ACAGGTGGAAAAATCCTTGCGGGAGAACGCTGAGCTCTCCATGTTGCTCCTCAAGAGCCTGTCTTCCCGCATCTTGCAGA
CGGAGATGATGATCGAGACGCTGGCCCACCGCGACATGGGATCCCGTCTGGTCAGCTTTCTACTGATCCTCTGCCGCGAC
TTCGGGGTGCCCGGCCCCAATGGAGTCACCATCGATCTCAAGCTATCCCACCAGGCCATTGCGGAAGCGATCGGCTCCAC
TCGCGTCACCATCACTCGTCTGTTGGGGGAGCTGCGCAAGAAGAACTACATTTCCATCCACAAGAAAAAGATCACCGTCC
ACGACCCAATGCAGCTTGGCCATCGCTTTGCCTAG

Upstream 100 bases:

>100_bases
GGTGGCTTGGGTCAATAGCGGGCAGCTCAACCCCGGCACTCCTGGCTGATCTTGTTGGGTTGATTCTAAGGGATAAACTG
ACCTTAAAAGGTTGAAGAAT

Downstream 100 bases:

>100_bases
GGGGAGAAGCTGCCCCTAGGGCCGTTTCCAGGAATTTACCAGTCGGGACAATCCCTCCCTCTGGGAGAGGAGTAGCCGTG
CCACCAGGGCAGGGCAGCGA

Product: nitrogen-responsive regulatory protein

Products: NA

Alternate protein names: DNA-binding protein VF1; Nitrogen-responsive regulatory protein [H]

Number of amino acids: Translated: 224; Mature: 223

Protein sequence:

>224_residues
MAVAIAEKSLVAAFKQLGGPHHPPVIETFERGKTIFFPGDPAERVYFLVKGAVKLSRVYEMGEEITVALLRENSIFGVLS
FITGQRSDRFYHAVAFTRVELLSLPIEQVEKSLRENAELSMLLLKSLSSRILQTEMMIETLAHRDMGSRLVSFLLILCRD
FGVPGPNGVTIDLKLSHQAIAEAIGSTRVTITRLLGELRKKNYISIHKKKITVHDPMQLGHRFA

Sequences:

>Translated_224_residues
MAVAIAEKSLVAAFKQLGGPHHPPVIETFERGKTIFFPGDPAERVYFLVKGAVKLSRVYEMGEEITVALLRENSIFGVLS
FITGQRSDRFYHAVAFTRVELLSLPIEQVEKSLRENAELSMLLLKSLSSRILQTEMMIETLAHRDMGSRLVSFLLILCRD
FGVPGPNGVTIDLKLSHQAIAEAIGSTRVTITRLLGELRKKNYISIHKKKITVHDPMQLGHRFA
>Mature_223_residues
AVAIAEKSLVAAFKQLGGPHHPPVIETFERGKTIFFPGDPAERVYFLVKGAVKLSRVYEMGEEITVALLRENSIFGVLSF
ITGQRSDRFYHAVAFTRVELLSLPIEQVEKSLRENAELSMLLLKSLSSRILQTEMMIETLAHRDMGSRLVSFLLILCRDF
GVPGPNGVTIDLKLSHQAIAEAIGSTRVTITRLLGELRKKNYISIHKKKITVHDPMQLGHRFA

Specific function: Has affinity for the xisA upstream region. Binds to a 66 bp region containing three repeats of the consensus recognition sequence 5'-ACATT-3' [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789756, Length=209, Percent_Identity=28.2296650717703, Blast_Score=75, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR022299
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 25083; Mature: 24952

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: PS50042 CNMP_BINDING_3 ; PS00042 HTH_CRP_1 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVAIAEKSLVAAFKQLGGPHHPPVIETFERGKTIFFPGDPAERVYFLVKGAVKLSRVYE
CEEEEHHHHHHHHHHHHCCCCCCCHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHH
MGEEITVALLRENSIFGVLSFITGQRSDRFYHAVAFTRVELLSLPIEQVEKSLRENAELS
CCHHHEEEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHH
MLLLKSLSSRILQTEMMIETLAHRDMGSRLVSFLLILCRDFGVPGPNGVTIDLKLSHQAI
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECHHHH
AEAIGSTRVTITRLLGELRKKNYISIHKKKITVHDPMQLGHRFA
HHHHCCCHHHHHHHHHHHHHCCCEEEEEEEEEEECHHHHCCCCC
>Mature Secondary Structure 
AVAIAEKSLVAAFKQLGGPHHPPVIETFERGKTIFFPGDPAERVYFLVKGAVKLSRVYE
EEEEHHHHHHHHHHHHCCCCCCCHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHH
MGEEITVALLRENSIFGVLSFITGQRSDRFYHAVAFTRVELLSLPIEQVEKSLRENAELS
CCHHHEEEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHH
MLLLKSLSSRILQTEMMIETLAHRDMGSRLVSFLLILCRDFGVPGPNGVTIDLKLSHQAI
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECHHHH
AEAIGSTRVTITRLLGELRKKNYISIHKKKITVHDPMQLGHRFA
HHHHCCCHHHHHHHHHHHHHCCCEEEEEEEEEEECHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11114927 [H]