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The map label for this gene is dnaN

Identifier: 86606304

GI number: 86606304

Start: 1646277

End: 1647641

Strand: Reverse

Name: dnaN

Synonym: CYA_1647

Alternate gene names: 86606304

Gene position: 1647641-1646277 (Counterclockwise)

Preceding gene: 86606306

Following gene: 86606300

Centisome position: 56.18

GC content: 62.12

Gene sequence:

>1365_bases
ATGGTGCAAGCTCAACGTCCTGCGGATTGGGAAGTCGAAACCGGATCGCCCGCGTCTGCCCCGCGCGACGGCAGGTCTGG
GGATCCCTTCCCCGTCAACAGGACAGAGTCCTTCGGCAAGGCTGTCGACCTACCTGCCGGTTGTCTTTGCTTCACCTGTG
AGCAAGCTGAGCTCAGCCACCACCTTGCTGCAGTTAGCCGAGCCGTTGCCAGCCGTCCTGCCCTGCCAGTTTTGGCCAAC
GTGCTGGTAGAGGCAAGTGCAGAGGCACAGCGGGTTGCCCTGACGGCCTTTGACTTGAACTTGGGCATTCGCAGCGAGTT
CGAGGCCGAGGTGAAGGGATCCGGTCGCACCACTCTGCCGGCCAAGCTGCTCAGCGACATCGTCAGCCGTTTGCCTAGCG
GCCCGATCGGCCTCAGCCAAGTGCCGGAGAGCGCTGCCGTGACCCTTACATCGGCCGCCGGCCAGTACCAGCTACGCGGC
ATGCCTGCGGAAGAGTATCCCACCTTGCCCGAGATAGCGCCCCGTACTGCCGATGCCGATGCTGAGGAGGAGAGCTTAGC
CGCCCAGATCCTTGAGCTGCCCATCGAGAGCCTGCTGCAAGGGATTGGCCAAACCCTCTTTGCCGCTTCCGGTGATGACA
CCAAGCAAGTGCTTACGGGTGTACATGTCAAGCTCTTGACCGGGGATCCGCCGCGGCTGGAGTTTGCGGCCACCGATGGA
CATCGCCTGGCGATAGTTCAGGTGGAGGCGGAAGGGATCCCAGTTGATTTGGCGGCTGGGGGGACGAAGCTGGATTTCAC
CATTCCCGCTCGGACTCTACGAGAGGTGGAGCGCATCCTCGGCGCCCAGACGGCCGCCTCCGCGGTGGAACTGCGGTTTG
ACCGGTCGCAGGTGCAATTTATTTTGCCCCCTGGCGCCGGCAGCGCTCAGTGCCAGCAGCGGATTACCAGCCGCCTTTTG
GAGGGCCAGTACCCCGACTACAACCGCCTGATCCCCCGGCAATTTGAGCGGCATGTAACCCTGGAGCGTAGGCCTTTGAT
CGAGTCCTTGGAGCGAGTGGGGGTGTTTGCTGCCCAGCGGAACGAGATCATCAAGTTCAACCTTTCTGAGGCAACCCAAA
CCCTACGCATCAGCACCGAGGCGCCCGATGTGGGCAGCGGCGAGGAATCTCTGCCGGTACAGATGTCCGGCCCTGACCTG
GAGCTGGCTTTTAATGTGCGCTACCTGCTGGAGGCCCTTAAGGTGTTTCATACACAGCAGGTGAGCCTGGAGCTGAACGG
CGCTACCCAGCCAGCCATCTGGAAGCCGATTGGAGCGACGCGCCTCTGCTATTTGGTCATGCCGGTTCAACTGCGGGCTA
CCTAG

Upstream 100 bases:

>100_bases
CGCGCGCGAGGAAAATTTTTTGCAAAACTCGTCTGGGCAGGGAGGGATCCGTGGGAAGCAGGCGGTGTAGGATAGGCGTA
TTCAGCTCGCAAGTCCCCTG

Downstream 100 bases:

>100_bases
CCCACCCCAGCCTGACCCAAATCGAAGATTGGCCTCAGTTGCTCCCCTGACCTGTCCGGAGAGGAGAACAGGTGGCTGCC
GATAGCAGCTCGAGCAAACC

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MVQAQRPADWEVETGSPASAPRDGRSGDPFPVNRTESFGKAVDLPAGCLCFTCEQAELSHHLAAVSRAVASRPALPVLAN
VLVEASAEAQRVALTAFDLNLGIRSEFEAEVKGSGRTTLPAKLLSDIVSRLPSGPIGLSQVPESAAVTLTSAAGQYQLRG
MPAEEYPTLPEIAPRTADADAEEESLAAQILELPIESLLQGIGQTLFAASGDDTKQVLTGVHVKLLTGDPPRLEFAATDG
HRLAIVQVEAEGIPVDLAAGGTKLDFTIPARTLREVERILGAQTAASAVELRFDRSQVQFILPPGAGSAQCQQRITSRLL
EGQYPDYNRLIPRQFERHVTLERRPLIESLERVGVFAAQRNEIIKFNLSEATQTLRISTEAPDVGSGEESLPVQMSGPDL
ELAFNVRYLLEALKVFHTQQVSLELNGATQPAIWKPIGATRLCYLVMPVQLRAT

Sequences:

>Translated_454_residues
MVQAQRPADWEVETGSPASAPRDGRSGDPFPVNRTESFGKAVDLPAGCLCFTCEQAELSHHLAAVSRAVASRPALPVLAN
VLVEASAEAQRVALTAFDLNLGIRSEFEAEVKGSGRTTLPAKLLSDIVSRLPSGPIGLSQVPESAAVTLTSAAGQYQLRG
MPAEEYPTLPEIAPRTADADAEEESLAAQILELPIESLLQGIGQTLFAASGDDTKQVLTGVHVKLLTGDPPRLEFAATDG
HRLAIVQVEAEGIPVDLAAGGTKLDFTIPARTLREVERILGAQTAASAVELRFDRSQVQFILPPGAGSAQCQQRITSRLL
EGQYPDYNRLIPRQFERHVTLERRPLIESLERVGVFAAQRNEIIKFNLSEATQTLRISTEAPDVGSGEESLPVQMSGPDL
ELAFNVRYLLEALKVFHTQQVSLELNGATQPAIWKPIGATRLCYLVMPVQLRAT
>Mature_454_residues
MVQAQRPADWEVETGSPASAPRDGRSGDPFPVNRTESFGKAVDLPAGCLCFTCEQAELSHHLAAVSRAVASRPALPVLAN
VLVEASAEAQRVALTAFDLNLGIRSEFEAEVKGSGRTTLPAKLLSDIVSRLPSGPIGLSQVPESAAVTLTSAAGQYQLRG
MPAEEYPTLPEIAPRTADADAEEESLAAQILELPIESLLQGIGQTLFAASGDDTKQVLTGVHVKLLTGDPPRLEFAATDG
HRLAIVQVEAEGIPVDLAAGGTKLDFTIPARTLREVERILGAQTAASAVELRFDRSQVQFILPPGAGSAQCQQRITSRLL
EGQYPDYNRLIPRQFERHVTLERRPLIESLERVGVFAAQRNEIIKFNLSEATQTLRISTEAPDVGSGEESLPVQMSGPDL
ELAFNVRYLLEALKVFHTQQVSLELNGATQPAIWKPIGATRLCYLVMPVQLRAT

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=405, Percent_Identity=25.679012345679, Blast_Score=115, Evalue=4e-27,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 48888; Mature: 48888

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQAQRPADWEVETGSPASAPRDGRSGDPFPVNRTESFGKAVDLPAGCLCFTCEQAELSH
CCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCEEEEEECHHHHHH
HLAAVSRAVASRPALPVLANVLVEASAEAQRVALTAFDLNLGIRSEFEAEVKGSGRTTLP
HHHHHHHHHHCCCCHHHHHHHHHHCCCCHHEEEEEEEEECCCCCCCCCCEECCCCCCCCH
AKLLSDIVSRLPSGPIGLSQVPESAAVTLTSAAGQYQLRGMPAEEYPTLPEIAPRTADAD
HHHHHHHHHHCCCCCCCHHHCCCCCEEEEEECCCCEEECCCCHHHCCCCCCCCCCCCCCC
AEEESLAAQILELPIESLLQGIGQTLFAASGDDTKQVLTGVHVKLLTGDPPRLEFAATDG
CHHHHHHHHHHHHHHHHHHHHHCHHHEECCCCCHHHHHCCCEEEEEECCCCCEEEEECCC
HRLAIVQVEAEGIPVDLAAGGTKLDFTIPARTLREVERILGAQTAASAVELRFDRSQVQF
CEEEEEEEECCCCCEEEECCCEEEEEECCHHHHHHHHHHHCCHHHHHHHEEEECCCCEEE
ILPPGAGSAQCQQRITSRLLEGQYPDYNRLIPRQFERHVTLERRPLIESLERVGVFAAQR
EECCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHCCC
NEIIKFNLSEATQTLRISTEAPDVGSGEESLPVQMSGPDLELAFNVRYLLEALKVFHTQQ
CCEEEEEHHHCCEEEEEECCCCCCCCCCCCCCEEECCCCEEEEECHHHHHHHHHHHHCEE
VSLELNGATQPAIWKPIGATRLCYLVMPVQLRAT
EEEEECCCCCCCCCCCCCHHEEEEEEEEEEEECC
>Mature Secondary Structure
MVQAQRPADWEVETGSPASAPRDGRSGDPFPVNRTESFGKAVDLPAGCLCFTCEQAELSH
CCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCEEEEEECHHHHHH
HLAAVSRAVASRPALPVLANVLVEASAEAQRVALTAFDLNLGIRSEFEAEVKGSGRTTLP
HHHHHHHHHHCCCCHHHHHHHHHHCCCCHHEEEEEEEEECCCCCCCCCCEECCCCCCCCH
AKLLSDIVSRLPSGPIGLSQVPESAAVTLTSAAGQYQLRGMPAEEYPTLPEIAPRTADAD
HHHHHHHHHHCCCCCCCHHHCCCCCEEEEEECCCCEEECCCCHHHCCCCCCCCCCCCCCC
AEEESLAAQILELPIESLLQGIGQTLFAASGDDTKQVLTGVHVKLLTGDPPRLEFAATDG
CHHHHHHHHHHHHHHHHHHHHHCHHHEECCCCCHHHHHCCCEEEEEECCCCCEEEEECCC
HRLAIVQVEAEGIPVDLAAGGTKLDFTIPARTLREVERILGAQTAASAVELRFDRSQVQF
CEEEEEEEECCCCCEEEECCCEEEEEECCHHHHHHHHHHHCCHHHHHHHEEEECCCCEEE
ILPPGAGSAQCQQRITSRLLEGQYPDYNRLIPRQFERHVTLERRPLIESLERVGVFAAQR
EECCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHCCC
NEIIKFNLSEATQTLRISTEAPDVGSGEESLPVQMSGPDLELAFNVRYLLEALKVFHTQQ
CCEEEEEHHHCCEEEEEECCCCCCCCCCCCCCEEECCCCEEEEECHHHHHHHHHHHHCEE
VSLELNGATQPAIWKPIGATRLCYLVMPVQLRAT
EEEEECCCCCCCCCCCCCHHEEEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]