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The map label for this gene is dus1 [H]

Identifier: 86606306

GI number: 86606306

Start: 1648460

End: 1649500

Strand: Reverse

Name: dus1 [H]

Synonym: CYA_1650

Alternate gene names: 86606306

Gene position: 1649500-1648460 (Counterclockwise)

Preceding gene: 86606307

Following gene: 86606304

Centisome position: 56.24

GC content: 58.5

Gene sequence:

>1041_bases
ATGGCCCAGCCCCTTGAGATTGGGGGGGTAAAGGTTTTTAGTCGTGTCTTTCAGGCACCCCTGTCAGGCGTAACCGACAA
AGTGTTTCGGGGGTTGGTACGCCGCTTTGGCCCGCAAAGCCTGGTCTACACCGAGATGGTGAACGCCACCGGGTTGCACT
ATGCCCGCGAGCTGCCCCGCATTATGGAAGTGGGTGAAGGAGAACAGCCCATCGGCATCCAGTTGTTCGACTGCCGGCCC
CACTTTTTGGCCGAAGCGGCTCGCATGGCCGTGGATGAGGGGGCGACCTTGGTGGATATCAACATGGGCTGCCCAGTAAA
CAAGATTACCAGAAACGGCGGCGGCTCTGCCCTGCTGCGGGAGCCGGACACGGCAGCCCGGATTGTGGAAGCGGTGGCCA
AGGCTGTACCTGTACCTGTTACAGTGAAAACTCGCCTGGGCTGGTCAGATCAGGAGATCAACATTCTGGATTTTGCCCAT
CGGCTGCAGGAGGCTGGAGCGCAACTGCTTACTTTGCATGGCCGCACACGCGCCCAGGGCTTTAGCGGCAAGGCTCGCTG
GGATTGGATCCGCCGGGTCAAAGAGCACTTACGCATTCCGGTGATCGCCAACGGCGATATTTTCTCGACTGATGCAGCGG
TAAAGTGTCTGGAGGAGACCGGAGCCGACGGGGTGATGTGCTCCCGCGGCACGATGGGCTACCCCTTCCTGGTGGGGCAA
ATCGATCACTTCCTGAAAACCGGCCAAGAAAGGCCGGCTCCTACCCCCCTGGATCGGATTCAAATGGCTTTAGAACACTT
GCGGGGCCTCTGGGAGTATAAGGGAGAACGTGGCATCCGTCAGGCCCGCAAGCACATGACCTGGTACATCAAGGATTTTG
CCGGCGCCTCTCAGTTTCGCGCCCTTCTCTGTCGCATCGAAACCTTGCAAGAAGGGGAAGCCCTTCTGCTTCAGGCCATG
GAGCGCTGCCAAGAGGCACCGCTGCAACCGGAAGAAGTAACTGAAGACTCAAACGAAGAGCAATTTGAAGAGACTACCTG
A

Upstream 100 bases:

>100_bases
CCTAGGCCTTCTCTACTTTGTCAACAAAGAAGGGTAAGTTGCTGTCTGCTTTCTCCGGATCCCGATGAGCCTGCAGCTTT
CGCCGCAATTGCAAGCTCGA

Downstream 100 bases:

>100_bases
AATTGAGTGGGGGATCCTTTTACCCTGACGGCTGGGCGGTAGCCAATCGGTCTTTGACCGCCGTTTGCAAACGGTCGTTT
TCATCTTGAAACAAGGCATC

Product: dihydrouridine synthase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 346; Mature: 345

Protein sequence:

>346_residues
MAQPLEIGGVKVFSRVFQAPLSGVTDKVFRGLVRRFGPQSLVYTEMVNATGLHYARELPRIMEVGEGEQPIGIQLFDCRP
HFLAEAARMAVDEGATLVDINMGCPVNKITRNGGGSALLREPDTAARIVEAVAKAVPVPVTVKTRLGWSDQEINILDFAH
RLQEAGAQLLTLHGRTRAQGFSGKARWDWIRRVKEHLRIPVIANGDIFSTDAAVKCLEETGADGVMCSRGTMGYPFLVGQ
IDHFLKTGQERPAPTPLDRIQMALEHLRGLWEYKGERGIRQARKHMTWYIKDFAGASQFRALLCRIETLQEGEALLLQAM
ERCQEAPLQPEEVTEDSNEEQFEETT

Sequences:

>Translated_346_residues
MAQPLEIGGVKVFSRVFQAPLSGVTDKVFRGLVRRFGPQSLVYTEMVNATGLHYARELPRIMEVGEGEQPIGIQLFDCRP
HFLAEAARMAVDEGATLVDINMGCPVNKITRNGGGSALLREPDTAARIVEAVAKAVPVPVTVKTRLGWSDQEINILDFAH
RLQEAGAQLLTLHGRTRAQGFSGKARWDWIRRVKEHLRIPVIANGDIFSTDAAVKCLEETGADGVMCSRGTMGYPFLVGQ
IDHFLKTGQERPAPTPLDRIQMALEHLRGLWEYKGERGIRQARKHMTWYIKDFAGASQFRALLCRIETLQEGEALLLQAM
ERCQEAPLQPEEVTEDSNEEQFEETT
>Mature_345_residues
AQPLEIGGVKVFSRVFQAPLSGVTDKVFRGLVRRFGPQSLVYTEMVNATGLHYARELPRIMEVGEGEQPIGIQLFDCRPH
FLAEAARMAVDEGATLVDINMGCPVNKITRNGGGSALLREPDTAARIVEAVAKAVPVPVTVKTRLGWSDQEINILDFAHR
LQEAGAQLLTLHGRTRAQGFSGKARWDWIRRVKEHLRIPVIANGDIFSTDAAVKCLEETGADGVMCSRGTMGYPFLVGQI
DHFLKTGQERPAPTPLDRIQMALEHLRGLWEYKGERGIRQARKHMTWYIKDFAGASQFRALLCRIETLQEGEALLLQAME
RCQEAPLQPEEVTEDSNEEQFEETT

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI40807366, Length=340, Percent_Identity=31.1764705882353, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI239788462, Length=246, Percent_Identity=30.0813008130081, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI239788483, Length=252, Percent_Identity=29.7619047619048, Blast_Score=108, Evalue=6e-24,
Organism=Homo sapiens, GI31742496, Length=251, Percent_Identity=30.6772908366534, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI8923374, Length=155, Percent_Identity=32.258064516129, Blast_Score=79, Evalue=9e-15,
Organism=Escherichia coli, GI1789660, Length=310, Percent_Identity=40, Blast_Score=218, Evalue=7e-58,
Organism=Escherichia coli, GI1788462, Length=235, Percent_Identity=36.1702127659575, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI145693211, Length=315, Percent_Identity=26.6666666666667, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI25144369, Length=222, Percent_Identity=32.8828828828829, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17507177, Length=238, Percent_Identity=30.2521008403361, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17543114, Length=252, Percent_Identity=25.3968253968254, Blast_Score=95, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6323560, Length=232, Percent_Identity=32.7586206896552, Blast_Score=113, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6323433, Length=325, Percent_Identity=27.3846153846154, Blast_Score=88, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323437, Length=224, Percent_Identity=29.4642857142857, Blast_Score=83, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24580595, Length=259, Percent_Identity=34.7490347490347, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI19920448, Length=259, Percent_Identity=34.7490347490347, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI19921524, Length=233, Percent_Identity=32.618025751073, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24585320, Length=249, Percent_Identity=30.1204819277108, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI45549423, Length=136, Percent_Identity=36.0294117647059, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 38579; Mature: 38448

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQPLEIGGVKVFSRVFQAPLSGVTDKVFRGLVRRFGPQSLVYTEMVNATGLHYARELPR
CCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
IMEVGEGEQPIGIQLFDCRPHFLAEAARMAVDEGATLVDINMGCPVNKITRNGGGSALLR
HHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHCCCCCCEEEE
EPDTAARIVEAVAKAVPVPVTVKTRLGWSDQEINILDFAHRLQEAGAQLLTLHGRTRAQG
CCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCHHEEEECCCHHCCC
FSGKARWDWIRRVKEHLRIPVIANGDIFSTDAAVKCLEETGADGVMCSRGTMGYPFLVGQ
CCCCHHHHHHHHHHHHCCCCEEECCCEECHHHHHHHHHHCCCCCEEECCCCCCCCHHHHH
IDHFLKTGQERPAPTPLDRIQMALEHLRGLWEYKGERGIRQARKHMTWYIKDFAGASQFR
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCHHHHH
ALLCRIETLQEGEALLLQAMERCQEAPLQPEEVTEDSNEEQFEETT
HHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHCCC
>Mature Secondary Structure 
AQPLEIGGVKVFSRVFQAPLSGVTDKVFRGLVRRFGPQSLVYTEMVNATGLHYARELPR
CCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
IMEVGEGEQPIGIQLFDCRPHFLAEAARMAVDEGATLVDINMGCPVNKITRNGGGSALLR
HHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHCCCCCCEEEE
EPDTAARIVEAVAKAVPVPVTVKTRLGWSDQEINILDFAHRLQEAGAQLLTLHGRTRAQG
CCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCHHEEEECCCHHCCC
FSGKARWDWIRRVKEHLRIPVIANGDIFSTDAAVKCLEETGADGVMCSRGTMGYPFLVGQ
CCCCHHHHHHHHHHHHCCCCEEECCCEECHHHHHHHHHHCCCCCEEECCCCCCCCHHHHH
IDHFLKTGQERPAPTPLDRIQMALEHLRGLWEYKGERGIRQARKHMTWYIKDFAGASQFR
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCHHHHH
ALLCRIETLQEGEALLLQAMERCQEAPLQPEEVTEDSNEEQFEETT
HHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]