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The map label for this gene is dnaB

Identifier: 86606214

GI number: 86606214

Start: 1559184

End: 1560959

Strand: Reverse

Name: dnaB

Synonym: CYA_1550

Alternate gene names: 86606214

Gene position: 1560959-1559184 (Counterclockwise)

Preceding gene: 86606215

Following gene: 86606213

Centisome position: 53.22

GC content: 60.3

Gene sequence:

>1776_bases
GTGATTAGCGATTACGACCTGACCGGTGGGGATCGGCTTCCGCCTCAGAACATAGAAGCTGAGGAGGAGATCTTGGGCGG
CATCCTCCTTGACCCTGAGGCGATGGCGCGGGTGGCGGAGCTCTTGCGGCCTGAGATGTTCTACCTCTCCGCCCACCAGG
AGATCTACCGGGCTGCTCTGCAGTTGTACAGCCAGGGCCAGCCCACTGACCTGACCACTGTTTCTGCCTGGTTGGCAGAT
CACCAGCTCTTGGAGCGGGTGGGAGGAACGGCGGCAATTCGCCGCCTAGTGGAGCAGACGGTTAGCAGTGTCAACATTGA
CCAGTATGCCCGTCTGGTGGTGGACAAATACATGCGCCGCCGGCTGATTCAAGTCAGCAACACCTTGGCGCGTCTGGCCT
ACGACACTAGCCAGCCTCTCCCTCAGCTCCTGGATCGAGCCGAGCAAGAGGTGTTCAGCCTCACCCAGGAACGGGTGCAG
CGCAGCCTTGTTCCAGCCTCGGAAGTTTTGGTAAACATCTTCTCAGAGTTGGAGGAGAGGTTCCAGAGCGGGGCTCAGAT
CCCTGGGATCCCGACCAAATTCATTGACCTCGACAACCTGACCCAGGGCTTGCAGCGGTCGGATTTGGTCATCCTAGCCG
GCCGTCCCTCGATGGGAAAGACCAGCCTGGCTCTTAACATTGCCCAAAATGTGGCTGCCTACGTCAAGCAACCGGTGGCC
ATCTTCAGCCTGGAGATGTCGCGGGAGCAACTGGTGCAGCGGCTCTTGGCCAGCGAGGCGCGCATTGATAGTAGCCGTCT
GCGATCCGGGCGCATCAGCGAGAATGAGTGGCAACGCCTGGGGCAGGCGATTGGCTTTTTGTCGCAACTGCCTTTGTTTA
TCGACGACACGCCCAACTGCACGGTTACAGAAATTCGCTCTAAGGCGCGGCGTCTGCAGGTGGAGCAAGGAGGGACGTTG
GGGTTGGTGTTGATCGACTACTTGCAGCTAATCCAGGGATCTGAGTCCGACAACCGGGTGCAGGAATTGTCGCGCATTAC
CCGCGGCCTCAAGGCGCTAGCCAAAGAGCTGATGGTGCCGGTGCTGGTGCTATCTCAGCTCAGCCGGGCAGTGGAGTCGC
GCTCAGACAAGCGTCCGCAACTGGCAGATTTGCGGGAGTCGGGTTGTCTAGCCGGCGATACCCGCGTGAGGGACGTGGAT
CGCGGCAAGATCTGGCGCCTGGAGCAACTGGCAGCCCTGCCGCCTTCAGCAGAACTGCCGCGCCTGCTCAGCCTCAATGG
GCGCGGGCGGCTGGTGCCCCAACAGCCAGTGAGGGTATTTTGCAGTGGGCGGCAGCCCACATGTCTTCTAAAAACCCGCC
TCAACTTTTCCATTCGTGCCACTGGCAACCACCCTTTCCTAACTCGGGACGGCTGGAAAACCCTCGACGAGCTGCAACTG
GAGGAGGAGGTGGCGGTGGTGGTGCAGGAGGGGTTGATCTGGGATCCCGTCGTCTTCATTAGCGAGCCGGGGGAGCCGCA
ACCGGTTTACGACATCGAGATGCCCCGCCACCACAACTTTGTGGCCAACGGCCTGCTGGTGCACAACAGCATCGAGCAGG
ATGCCGACGTGGTGATGATGATCTACCGGCCCGAGTACTACGATCCCAACACCAGCGATCGCGGCATTGCCGAGGTGATC
GTCGCCAAACATCGCAACGGCCCCACGGGCACGGTGCGCCTTTTGTTTGAAAGCCAGTACACCCAGTTTCGCAACTTGGC
GGATCCCTATCGGTGA

Upstream 100 bases:

>100_bases
CTGGTTCAGACGGCTATGCCCACTTGGCCCGCTGCGCTAGGGTGTGCAGCCTAGCAGTCGGAGAGCGGGGGAGAGCTTGG
TTTTTCTACCCTGGCTGGTG

Downstream 100 bases:

>100_bases
GCGAAAGCCGCAGCAGAAGTAGATCGGGACTCCTTTCAAGGACAGGTCCGGGGTGCGAGGTGTTAGCCTGAGATTGCCCC
CCTAGGGCAGGTCGCTGTTG

Product: replicative DNA helicase

Products: NA

Alternate protein names: Asp dnaB intein [H]

Number of amino acids: Translated: 591; Mature: 591

Protein sequence:

>591_residues
MISDYDLTGGDRLPPQNIEAEEEILGGILLDPEAMARVAELLRPEMFYLSAHQEIYRAALQLYSQGQPTDLTTVSAWLAD
HQLLERVGGTAAIRRLVEQTVSSVNIDQYARLVVDKYMRRRLIQVSNTLARLAYDTSQPLPQLLDRAEQEVFSLTQERVQ
RSLVPASEVLVNIFSELEERFQSGAQIPGIPTKFIDLDNLTQGLQRSDLVILAGRPSMGKTSLALNIAQNVAAYVKQPVA
IFSLEMSREQLVQRLLASEARIDSSRLRSGRISENEWQRLGQAIGFLSQLPLFIDDTPNCTVTEIRSKARRLQVEQGGTL
GLVLIDYLQLIQGSESDNRVQELSRITRGLKALAKELMVPVLVLSQLSRAVESRSDKRPQLADLRESGCLAGDTRVRDVD
RGKIWRLEQLAALPPSAELPRLLSLNGRGRLVPQQPVRVFCSGRQPTCLLKTRLNFSIRATGNHPFLTRDGWKTLDELQL
EEEVAVVVQEGLIWDPVVFISEPGEPQPVYDIEMPRHHNFVANGLLVHNSIEQDADVVMMIYRPEYYDPNTSDRGIAEVI
VAKHRNGPTGTVRLLFESQYTQFRNLADPYR

Sequences:

>Translated_591_residues
MISDYDLTGGDRLPPQNIEAEEEILGGILLDPEAMARVAELLRPEMFYLSAHQEIYRAALQLYSQGQPTDLTTVSAWLAD
HQLLERVGGTAAIRRLVEQTVSSVNIDQYARLVVDKYMRRRLIQVSNTLARLAYDTSQPLPQLLDRAEQEVFSLTQERVQ
RSLVPASEVLVNIFSELEERFQSGAQIPGIPTKFIDLDNLTQGLQRSDLVILAGRPSMGKTSLALNIAQNVAAYVKQPVA
IFSLEMSREQLVQRLLASEARIDSSRLRSGRISENEWQRLGQAIGFLSQLPLFIDDTPNCTVTEIRSKARRLQVEQGGTL
GLVLIDYLQLIQGSESDNRVQELSRITRGLKALAKELMVPVLVLSQLSRAVESRSDKRPQLADLRESGCLAGDTRVRDVD
RGKIWRLEQLAALPPSAELPRLLSLNGRGRLVPQQPVRVFCSGRQPTCLLKTRLNFSIRATGNHPFLTRDGWKTLDELQL
EEEVAVVVQEGLIWDPVVFISEPGEPQPVYDIEMPRHHNFVANGLLVHNSIEQDADVVMMIYRPEYYDPNTSDRGIAEVI
VAKHRNGPTGTVRLLFESQYTQFRNLADPYR
>Mature_591_residues
MISDYDLTGGDRLPPQNIEAEEEILGGILLDPEAMARVAELLRPEMFYLSAHQEIYRAALQLYSQGQPTDLTTVSAWLAD
HQLLERVGGTAAIRRLVEQTVSSVNIDQYARLVVDKYMRRRLIQVSNTLARLAYDTSQPLPQLLDRAEQEVFSLTQERVQ
RSLVPASEVLVNIFSELEERFQSGAQIPGIPTKFIDLDNLTQGLQRSDLVILAGRPSMGKTSLALNIAQNVAAYVKQPVA
IFSLEMSREQLVQRLLASEARIDSSRLRSGRISENEWQRLGQAIGFLSQLPLFIDDTPNCTVTEIRSKARRLQVEQGGTL
GLVLIDYLQLIQGSESDNRVQELSRITRGLKALAKELMVPVLVLSQLSRAVESRSDKRPQLADLRESGCLAGDTRVRDVD
RGKIWRLEQLAALPPSAELPRLLSLNGRGRLVPQQPVRVFCSGRQPTCLLKTRLNFSIRATGNHPFLTRDGWKTLDELQL
EEEVAVVVQEGLIWDPVVFISEPGEPQPVYDIEMPRHHNFVANGLLVHNSIEQDADVVMMIYRPEYYDPNTSDRGIAEVI
VAKHRNGPTGTVRLLFESQYTQFRNLADPYR

Specific function: The intein is an endonuclease (Potential) [H]

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=392, Percent_Identity=41.8367346938776, Blast_Score=291, Evalue=6e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136
- InterPro:   IPR003586
- InterPro:   IPR003587
- InterPro:   IPR006142
- InterPro:   IPR004042
- InterPro:   IPR006141 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 66500; Mature: 66500

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS50817 INTEIN_N_TER ; PS50818 INTEIN_C_TER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISDYDLTGGDRLPPQNIEAEEEILGGILLDPEAMARVAELLRPEMFYLSAHQEIYRAAL
CCCCCCCCCCCCCCCCCCCHHHHHHCCEEECHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
QLYSQGQPTDLTTVSAWLADHQLLERVGGTAAIRRLVEQTVSSVNIDQYARLVVDKYMRR
HHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
RLIQVSNTLARLAYDTSQPLPQLLDRAEQEVFSLTQERVQRSLVPASEVLVNIFSELEER
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
FQSGAQIPGIPTKFIDLDNLTQGLQRSDLVILAGRPSMGKTSLALNIAQNVAAYVKQPVA
HHCCCCCCCCCHHHCCHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCHH
IFSLEMSREQLVQRLLASEARIDSSRLRSGRISENEWQRLGQAIGFLSQLPLFIDDTPNC
EEEHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHHHCCEEECCCCCC
TVTEIRSKARRLQVEQGGTLGLVLIDYLQLIQGSESDNRVQELSRITRGLKALAKELMVP
HHHHHHHHHHHEECCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VLVLSQLSRAVESRSDKRPQLADLRESGCLAGDTRVRDVDRGKIWRLEQLAALPPSAELP
HHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCEEHHHHHCCCCCCCCC
RLLSLNGRGRLVPQQPVRVFCSGRQPTCLLKTRLNFSIRATGNHPFLTRDGWKTLDELQL
HHHCCCCCCCCCCCCCHHHEECCCCCEEEEEECCCEEEEECCCCCCEECCCHHHHHHHHH
EEEVAVVVQEGLIWDPVVFISEPGEPQPVYDIEMPRHHNFVANGLLVHNSIEQDADVVMM
HHHHHHHHHCCCCCCCEEEEECCCCCCCEEECCCCCCCCHHHCCEEEECCCCCCCCEEEE
IYRPEYYDPNTSDRGIAEVIVAKHRNGPTGTVRLLFESQYTQFRNLADPYR
EEECCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHCCCCCC
>Mature Secondary Structure
MISDYDLTGGDRLPPQNIEAEEEILGGILLDPEAMARVAELLRPEMFYLSAHQEIYRAAL
CCCCCCCCCCCCCCCCCCCHHHHHHCCEEECHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
QLYSQGQPTDLTTVSAWLADHQLLERVGGTAAIRRLVEQTVSSVNIDQYARLVVDKYMRR
HHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
RLIQVSNTLARLAYDTSQPLPQLLDRAEQEVFSLTQERVQRSLVPASEVLVNIFSELEER
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
FQSGAQIPGIPTKFIDLDNLTQGLQRSDLVILAGRPSMGKTSLALNIAQNVAAYVKQPVA
HHCCCCCCCCCHHHCCHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCHH
IFSLEMSREQLVQRLLASEARIDSSRLRSGRISENEWQRLGQAIGFLSQLPLFIDDTPNC
EEEHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHHHCCEEECCCCCC
TVTEIRSKARRLQVEQGGTLGLVLIDYLQLIQGSESDNRVQELSRITRGLKALAKELMVP
HHHHHHHHHHHEECCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VLVLSQLSRAVESRSDKRPQLADLRESGCLAGDTRVRDVDRGKIWRLEQLAALPPSAELP
HHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCEEHHHHHCCCCCCCCC
RLLSLNGRGRLVPQQPVRVFCSGRQPTCLLKTRLNFSIRATGNHPFLTRDGWKTLDELQL
HHHCCCCCCCCCCCCCHHHEECCCCCEEEEEECCCEEEEECCCCCCEECCCHHHHHHHHH
EEEVAVVVQEGLIWDPVVFISEPGEPQPVYDIEMPRHHNFVANGLLVHNSIEQDADVVMM
HHHHHHHHHCCCCCCCEEEEECCCCCCCEEECCCCCCCCHHHCCEEEECCCCCCCCEEEE
IYRPEYYDPNTSDRGIAEVIVAKHRNGPTGTVRLLFESQYTQFRNLADPYR
EEECCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]