Click here to switch to the map view.

The map label for this gene is lspA

Identifier: 86606198

GI number: 86606198

Start: 1544448

End: 1544918

Strand: Reverse

Name: lspA

Synonym: CYA_1533

Alternate gene names: 86606198

Gene position: 1544918-1544448 (Counterclockwise)

Preceding gene: 86606199

Following gene: 86606196

Centisome position: 52.68

GC content: 62.42

Gene sequence:

>471_bases
ATGAAAAACACGGGCTTTTGGCTGGCCGGGATCCTGGGGTTGCTCCTGGATCAGGCGAGCAAGCGCTGGGCGGTGGAGCG
GCTGCTGCCGGGGCCGGAAATCGAGCTGTGGCCAGGGGTGTTTCACCTTACCTACGTGGAAAATACCGGCGCCGCTTGGA
GCCTATTTGCCGATTCGGCAGAGTTCCTCAAGTGGGTCTCGCTGGGGGTAGCGGTGGCGCTGGCGCTGCTGGCGATTCGC
GGCAGCAGCCTGGGCCGTTGGGAACAGGTGGGCTACGGCTTGGTCTGGGCAGGAGCGATGGGGAACGGCATCGACCGCTT
TCTCAACGGCTATGTGGTGGATTTTTTCAACTTCGTCTGGATTGGCTTCCCCGTCTTTAACGTGGCGGACGTGGCCATCA
ACGTGGGGGTGGCCTGCTTGTTGGTTGGCCTGTGGCAGGGCAGCCCGAAAAAAACGGCGCCGAGACCCTAG

Upstream 100 bases:

>100_bases
GCTCCTGCGGTTTGCCGCTCACAAAAGAAGAGAGGATGTCAGGATGGGAAAAGTGCTCCTCTGAGCGCTCTTGCCGGTGG
ACGTAGTGAGGTGAAAAAGC

Downstream 100 bases:

>100_bases
GCGACCTACTTCGCCCCCGCCCACTGGCGATCCCCAGCTTCTTGCGCGGCCAACTCAGGCTGCGAGGGAGCCTCAGGATC
CGGCGGAGCGCTCCACCACA

Product: lipoprotein signal peptidase

Products: NA

Alternate protein names: Prolipoprotein signal peptidase; Signal peptidase II; SPase II [H]

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MKNTGFWLAGILGLLLDQASKRWAVERLLPGPEIELWPGVFHLTYVENTGAAWSLFADSAEFLKWVSLGVAVALALLAIR
GSSLGRWEQVGYGLVWAGAMGNGIDRFLNGYVVDFFNFVWIGFPVFNVADVAINVGVACLLVGLWQGSPKKTAPRP

Sequences:

>Translated_156_residues
MKNTGFWLAGILGLLLDQASKRWAVERLLPGPEIELWPGVFHLTYVENTGAAWSLFADSAEFLKWVSLGVAVALALLAIR
GSSLGRWEQVGYGLVWAGAMGNGIDRFLNGYVVDFFNFVWIGFPVFNVADVAINVGVACLLVGLWQGSPKKTAPRP
>Mature_156_residues
MKNTGFWLAGILGLLLDQASKRWAVERLLPGPEIELWPGVFHLTYVENTGAAWSLFADSAEFLKWVSLGVAVALALLAIR
GSSLGRWEQVGYGLVWAGAMGNGIDRFLNGYVVDFFNFVWIGFPVFNVADVAINVGVACLLVGLWQGSPKKTAPRP

Specific function: This protein specifically catalyzes the removal of signal peptides from prolipoproteins [H]

COG id: COG0597

COG function: function code MU; Lipoprotein signal peptidase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A8 family [H]

Homologues:

Organism=Escherichia coli, GI1786210, Length=146, Percent_Identity=34.9315068493151, Blast_Score=80, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001872 [H]

Pfam domain/function: PF01252 Peptidase_A8 [H]

EC number: =3.4.23.36 [H]

Molecular weight: Translated: 16996; Mature: 16996

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: PS00855 SPASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNTGFWLAGILGLLLDQASKRWAVERLLPGPEIELWPGVFHLTYVENTGAAWSLFADSA
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCEEEEEEEECCCCEEEHHHHHH
EFLKWVSLGVAVALALLAIRGSSLGRWEQVGYGLVWAGAMGNGIDRFLNGYVVDFFNFVW
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHH
IGFPVFNVADVAINVGVACLLVGLWQGSPKKTAPRP
HHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKNTGFWLAGILGLLLDQASKRWAVERLLPGPEIELWPGVFHLTYVENTGAAWSLFADSA
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCEEEEEEEECCCCEEEHHHHHH
EFLKWVSLGVAVALALLAIRGSSLGRWEQVGYGLVWAGAMGNGIDRFLNGYVVDFFNFVW
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHH
IGFPVFNVADVAINVGVACLLVGLWQGSPKKTAPRP
HHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 14621292 [H]