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The map label for this gene is gmd

Identifier: 86606185

GI number: 86606185

Start: 1530410

End: 1531546

Strand: Reverse

Name: gmd

Synonym: CYA_1520

Alternate gene names: 86606185

Gene position: 1531546-1530410 (Counterclockwise)

Preceding gene: 86606186

Following gene: 86606184

Centisome position: 52.22

GC content: 61.48

Gene sequence:

>1137_bases
ATGACCCAACGTGCCTTAATTACCGGCATCACCGGCCAGGATGGCTCTTACCTGGCCGAGCTGCTGTTGCAAAAAAACTA
TGAAGTCCACGGCATTGTCCGCCGCGCCAGCACCTTCAATACCGACCGCATCGACCACATCTATGTGGATGCCCACGATC
CCAACGCCCGGCTGTTTCTCCACTATGGGGATCTCACCGATGGCACCACCCTGCGCAAGATCCTGGAACAGGTGCAGCCG
ACGGAGGTGTATAACCTGGGGGCCCAATCCCACGTGCGGGTCAGCTTTGAGGCGCCAGAATACACCGTCGATGCGGTGGC
TATGGGCACCCTACGCCTCCTGGAGGCCATCCGCGACTACCAGCAGCGCACGGGGATCCGGGTGAAGTTTTACCAGGCGG
GATCCTCGGAGATGTTCGGCCTGGTGCAGGAGATCCCGCAAAAGGAGACCACCCCCTTCTACCCCCGCAGCCCCTACGCC
TGCGCCAAGGTGTACGCCTACTGGCAAACAGTCAACTACCGCGAAGCCTACAACCTCTTTGCCTGCAACGGCATTTTGTT
CAACCACGAGAGCCCACGGCGAGGAGAAACCTTTGTTACCCGCAAGATCACCCGCGCCGTCAGCCGCATCGTGGCCGGCA
AGCAGGACAAGCTCTACCTGGGCAACCTGGATGCCAAGCGGGACTGGGGCTACGCCAAGGACTACGTCGAGGGGATGTGG
ATGATGCTGCAACAACCCCAGCCGGACGACTACGTGCTGGCTACTGGCGAAACCCACAGCGTGCGGGAGTTTGCCGAACG
CGCTTTTGCTTTGGTGGGCATGCCCATTACCTGGCGGGGATCCGGACTGGAAGAACAGGGACTCTACAAAGATCGCGTGC
TGATCGAGATCGATCCCCGCTACTACCGCCCCACGGAGGTGGATCTGCTCTTGGGGGACGCCTCCAAGGCCAAGCGGGTA
CTGGGGTGGCAGCCCAAGGTGACCTTTGCCCAACTGGTGGAGCTGATGGTGCTGGCGGATCTGGAGGCCATCGGCTTGGA
CCCGGATCCCATCCTGGGGGAGCGGCGCACCCTCAACGGCCTGCTGAGCGAGGATCGCGCCTTCATCCGCACCCAACTGG
GGGTTCGCTACGACTAA

Upstream 100 bases:

>100_bases
TGGCTTTGGGGTTTCAGCTTTGCGAGTTTGTGCTTGGCAAGGCGCTGAAGTCCCCGCTCTGGCTGAAGTCTTACAAGTGC
TTGTTTTCGCCTCCAGCTCT

Downstream 100 bases:

>100_bases
TGGCACCGCTCCCCAAAGTCAGCTAAAAAACGGCTCTCACCCCTTGCTTAAAGGCCGGGCTAAGACCTTAATATAGGAGA
TCGCCAATTCTTTTGGCGCA

Product: GDP-mannose 4,6-dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase [H]

Number of amino acids: Translated: 378; Mature: 377

Protein sequence:

>378_residues
MTQRALITGITGQDGSYLAELLLQKNYEVHGIVRRASTFNTDRIDHIYVDAHDPNARLFLHYGDLTDGTTLRKILEQVQP
TEVYNLGAQSHVRVSFEAPEYTVDAVAMGTLRLLEAIRDYQQRTGIRVKFYQAGSSEMFGLVQEIPQKETTPFYPRSPYA
CAKVYAYWQTVNYREAYNLFACNGILFNHESPRRGETFVTRKITRAVSRIVAGKQDKLYLGNLDAKRDWGYAKDYVEGMW
MMLQQPQPDDYVLATGETHSVREFAERAFALVGMPITWRGSGLEEQGLYKDRVLIEIDPRYYRPTEVDLLLGDASKAKRV
LGWQPKVTFAQLVELMVLADLEAIGLDPDPILGERRTLNGLLSEDRAFIRTQLGVRYD

Sequences:

>Translated_378_residues
MTQRALITGITGQDGSYLAELLLQKNYEVHGIVRRASTFNTDRIDHIYVDAHDPNARLFLHYGDLTDGTTLRKILEQVQP
TEVYNLGAQSHVRVSFEAPEYTVDAVAMGTLRLLEAIRDYQQRTGIRVKFYQAGSSEMFGLVQEIPQKETTPFYPRSPYA
CAKVYAYWQTVNYREAYNLFACNGILFNHESPRRGETFVTRKITRAVSRIVAGKQDKLYLGNLDAKRDWGYAKDYVEGMW
MMLQQPQPDDYVLATGETHSVREFAERAFALVGMPITWRGSGLEEQGLYKDRVLIEIDPRYYRPTEVDLLLGDASKAKRV
LGWQPKVTFAQLVELMVLADLEAIGLDPDPILGERRTLNGLLSEDRAFIRTQLGVRYD
>Mature_377_residues
TQRALITGITGQDGSYLAELLLQKNYEVHGIVRRASTFNTDRIDHIYVDAHDPNARLFLHYGDLTDGTTLRKILEQVQPT
EVYNLGAQSHVRVSFEAPEYTVDAVAMGTLRLLEAIRDYQQRTGIRVKFYQAGSSEMFGLVQEIPQKETTPFYPRSPYAC
AKVYAYWQTVNYREAYNLFACNGILFNHESPRRGETFVTRKITRAVSRIVAGKQDKLYLGNLDAKRDWGYAKDYVEGMWM
MLQQPQPDDYVLATGETHSVREFAERAFALVGMPITWRGSGLEEQGLYKDRVLIEIDPRYYRPTEVDLLLGDASKAKRVL
GWQPKVTFAQLVELMVLADLEAIGLDPDPILGERRTLNGLLSEDRAFIRTQLGVRYD

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=351, Percent_Identity=61.5384615384615, Blast_Score=447, Evalue=1e-125,
Organism=Escherichia coli, GI1788366, Length=357, Percent_Identity=62.1848739495798, Blast_Score=464, Evalue=1e-132,
Organism=Escherichia coli, GI1788353, Length=348, Percent_Identity=24.4252873563218, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI48994969, Length=343, Percent_Identity=26.2390670553936, Blast_Score=78, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17539424, Length=347, Percent_Identity=60.2305475504323, Blast_Score=447, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI133901790, Length=347, Percent_Identity=60.2305475504323, Blast_Score=447, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI133901788, Length=347, Percent_Identity=60.2305475504323, Blast_Score=447, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI17539422, Length=347, Percent_Identity=60.2305475504323, Blast_Score=447, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI133901786, Length=347, Percent_Identity=60.2305475504323, Blast_Score=446, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI17507723, Length=347, Percent_Identity=59.6541786743516, Blast_Score=444, Evalue=1e-125,
Organism=Drosophila melanogaster, GI24158427, Length=348, Percent_Identity=62.6436781609195, Blast_Score=460, Evalue=1e-130,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 43182; Mature: 43050

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQRALITGITGQDGSYLAELLLQKNYEVHGIVRRASTFNTDRIDHIYVDAHDPNARLFL
CCCCEEEEEECCCCHHHHHHHHHHCCCCCEEEEEHHCCCCCCCCEEEEEECCCCCCEEEE
HYGDLTDGTTLRKILEQVQPTEVYNLGAQSHVRVSFEAPEYTVDAVAMGTLRLLEAIRDY
EECCCCCCHHHHHHHHHCCCCCEEECCCCCEEEEEECCCCEEEHHHHHHHHHHHHHHHHH
QQRTGIRVKFYQAGSSEMFGLVQEIPQKETTPFYPRSPYACAKVYAYWQTVNYREAYNLF
HHHCCCEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHCCEE
ACNGILFNHESPRRGETFVTRKITRAVSRIVAGKQDKLYLGNLDAKRDWGYAKDYVEGMW
EECEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHH
MMLQQPQPDDYVLATGETHSVREFAERAFALVGMPITWRGSGLEEQGLYKDRVLIEIDPR
HHHCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCEEEEEECCC
YYRPTEVDLLLGDASKAKRVLGWQPKVTFAQLVELMVLADLEAIGLDPDPILGERRTLNG
CCCCCEEEEEECCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
LLSEDRAFIRTQLGVRYD
HHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TQRALITGITGQDGSYLAELLLQKNYEVHGIVRRASTFNTDRIDHIYVDAHDPNARLFL
CCCEEEEEECCCCHHHHHHHHHHCCCCCEEEEEHHCCCCCCCCEEEEEECCCCCCEEEE
HYGDLTDGTTLRKILEQVQPTEVYNLGAQSHVRVSFEAPEYTVDAVAMGTLRLLEAIRDY
EECCCCCCHHHHHHHHHCCCCCEEECCCCCEEEEEECCCCEEEHHHHHHHHHHHHHHHHH
QQRTGIRVKFYQAGSSEMFGLVQEIPQKETTPFYPRSPYACAKVYAYWQTVNYREAYNLF
HHHCCCEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHCCEE
ACNGILFNHESPRRGETFVTRKITRAVSRIVAGKQDKLYLGNLDAKRDWGYAKDYVEGMW
EECEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHH
MMLQQPQPDDYVLATGETHSVREFAERAFALVGMPITWRGSGLEEQGLYKDRVLIEIDPR
HHHCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCEEEEEECCC
YYRPTEVDLLLGDASKAKRVLGWQPKVTFAQLVELMVLADLEAIGLDPDPILGERRTLNG
CCCCCEEEEEECCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
LLSEDRAFIRTQLGVRYD
HHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]