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The map label for this gene is metG

Identifier: 86606068

GI number: 86606068

Start: 1402734

End: 1404284

Strand: Reverse

Name: metG

Synonym: CYA_1398

Alternate gene names: 86606068

Gene position: 1404284-1402734 (Counterclockwise)

Preceding gene: 86606072

Following gene: 86606065

Centisome position: 47.88

GC content: 60.41

Gene sequence:

>1551_bases
ATGCCGAATTCCACCTTCGTCATCACCACGCCGATCTACTACGTCAACGCTGCCCCCCACATCGGCAGCGCCTACACCAC
CCTGGCGGCGGATGCCATCGCCCGTTACCATCGCCTGCGGGGCAAGGATGTGCTGATGGTAACGGGAACCGACGAGCACG
GCCAGAAGATCCAGCGCACCGCCGAAGAGCGCGGCCTTCCCCCGCAGCAGCACTGCGACGAGATCGCGGCCCAGTTTGCC
CAGCTTTGGCAGTTGCTGGACATCCGCTACGACCGCTTTATCCGCACCACCGACCCTCGCCACAAAGCCATTGTGCGGGA
GTTTTTCCAGCGAGTGTGGGATCGGGGGGATATTTTCGTTGGCCGGCAGCAGGGCTGGTACTGTGTTGCCTGCGAGGAGT
TCAAGGACGAGTCAGAGCTCTTAGAAGGCGGATTTTGCCCGCTTCACCCCAACAAAAAAGTGGAGTGGAAAGACGAAGAA
AACTACTTTTTTCGCCTCTCTCGCTACCAGGAAAAACTGGAAGCCCTCTATGCGGAGCACCCGGAGTTCATTCAACCAGA
AAGCCGCCGCAATGAGGTGATCAACTTTGTCAAGCAGGGCCTGCGGGACTTCTCCATCTCTCGCCCCAGCGTCTCCTGGG
GGATCCCCTTCCCGGCGGATCCCAGCCAAACCCTTTACGTCTGGTTTGATGCCCTCATCAACTACCTCAGCGCCGCCGTG
GATCCCGAGGACGAGCCGACGCTGGAAAACGCCTTGCGCCGCTACTGGCCCGCCGATGTGCACCTAGTGGGCAAAGACAT
TGTCCGCTTCCACGCCATCTACTGGCCGGCCATGCTCATGTCGGCAGGGCTGCCGCTGCCCAGACAGATCTTTGGCCACG
GCTTTCTCACCAAAGATGGCCTCAAGATGGGCAAAAGCCTGGGCAACACGCTGGATCCCTTTGAGCTGGTGCAGCGCTAC
GGTGCCGATGCCGTGCGCTACTATTTCTTGACTGAAATTGAGCTGGGCAAGGATGGAGACTTTAGTGAGGGCCGCTTCAT
CGAGGTTCTCAACGCTGACCTAGCCAACGACCTGGGGAACCTGCTCAACCGCACCCTCAATATGCTCAAAAAATACCAGC
CCGACTACCGCATCCCCGCCGTCGAGATCCCTGCGGATCACCCCCTCAAAACCCTGGCCGAGCGCCTGGCCCCGACAGTG
GCCGACGCCTACGAGCGGCTGGATCCCAGCTCTGCCGCCCGCAAAGCTCTCAGCTTGGCCCAGGCCAGCAACAAATACCT
CGACGAGCAGGCCCCCTGGACGTTGCACAAACAAGGGCAAAGCGAGCAGGTGGCCCAGATCCTCTACGCCGTTTTGGAAA
GCGTGCGCTGGGTGGCCGTGGCCCTTTCGCCCATCATTCCCAACCTCAGCCTCAAGATCCTGCAGCAGATGGGGTTTCCT
TTGCAGGATGTCGCACAACTGCGCTGGGATCCCGATGCCCGTTGGGGCCGGCTTCCGCCTGGGCAAGAGCCCCAACCTCC
TGTGCCCGTCTTCCAGAGAATACTCAAGTAG

Upstream 100 bases:

>100_bases
CTTGAGGTTGAACAGAAGTTTCATCCCACCTCCGCCCCGTAACTGGGCCTGTCGGACTTTCCGGCAACTTTTATCATGGG
GGAAAGCGCTGCGAGTGCTC

Downstream 100 bases:

>100_bases
CTCCTAAGAATTGGCGGCATCGGGTTGGGAAGGCTGGGATCCCTCTGGTGTTGCCTTGCCGGCGGAAAGACCCATATCTC
CCAAGGAAATGCTTGGCACA

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 516; Mature: 515

Protein sequence:

>516_residues
MPNSTFVITTPIYYVNAAPHIGSAYTTLAADAIARYHRLRGKDVLMVTGTDEHGQKIQRTAEERGLPPQQHCDEIAAQFA
QLWQLLDIRYDRFIRTTDPRHKAIVREFFQRVWDRGDIFVGRQQGWYCVACEEFKDESELLEGGFCPLHPNKKVEWKDEE
NYFFRLSRYQEKLEALYAEHPEFIQPESRRNEVINFVKQGLRDFSISRPSVSWGIPFPADPSQTLYVWFDALINYLSAAV
DPEDEPTLENALRRYWPADVHLVGKDIVRFHAIYWPAMLMSAGLPLPRQIFGHGFLTKDGLKMGKSLGNTLDPFELVQRY
GADAVRYYFLTEIELGKDGDFSEGRFIEVLNADLANDLGNLLNRTLNMLKKYQPDYRIPAVEIPADHPLKTLAERLAPTV
ADAYERLDPSSAARKALSLAQASNKYLDEQAPWTLHKQGQSEQVAQILYAVLESVRWVAVALSPIIPNLSLKILQQMGFP
LQDVAQLRWDPDARWGRLPPGQEPQPPVPVFQRILK

Sequences:

>Translated_516_residues
MPNSTFVITTPIYYVNAAPHIGSAYTTLAADAIARYHRLRGKDVLMVTGTDEHGQKIQRTAEERGLPPQQHCDEIAAQFA
QLWQLLDIRYDRFIRTTDPRHKAIVREFFQRVWDRGDIFVGRQQGWYCVACEEFKDESELLEGGFCPLHPNKKVEWKDEE
NYFFRLSRYQEKLEALYAEHPEFIQPESRRNEVINFVKQGLRDFSISRPSVSWGIPFPADPSQTLYVWFDALINYLSAAV
DPEDEPTLENALRRYWPADVHLVGKDIVRFHAIYWPAMLMSAGLPLPRQIFGHGFLTKDGLKMGKSLGNTLDPFELVQRY
GADAVRYYFLTEIELGKDGDFSEGRFIEVLNADLANDLGNLLNRTLNMLKKYQPDYRIPAVEIPADHPLKTLAERLAPTV
ADAYERLDPSSAARKALSLAQASNKYLDEQAPWTLHKQGQSEQVAQILYAVLESVRWVAVALSPIIPNLSLKILQQMGFP
LQDVAQLRWDPDARWGRLPPGQEPQPPVPVFQRILK
>Mature_515_residues
PNSTFVITTPIYYVNAAPHIGSAYTTLAADAIARYHRLRGKDVLMVTGTDEHGQKIQRTAEERGLPPQQHCDEIAAQFAQ
LWQLLDIRYDRFIRTTDPRHKAIVREFFQRVWDRGDIFVGRQQGWYCVACEEFKDESELLEGGFCPLHPNKKVEWKDEEN
YFFRLSRYQEKLEALYAEHPEFIQPESRRNEVINFVKQGLRDFSISRPSVSWGIPFPADPSQTLYVWFDALINYLSAAVD
PEDEPTLENALRRYWPADVHLVGKDIVRFHAIYWPAMLMSAGLPLPRQIFGHGFLTKDGLKMGKSLGNTLDPFELVQRYG
ADAVRYYFLTEIELGKDGDFSEGRFIEVLNADLANDLGNLLNRTLNMLKKYQPDYRIPAVEIPADHPLKTLAERLAPTVA
DAYERLDPSSAARKALSLAQASNKYLDEQAPWTLHKQGQSEQVAQILYAVLESVRWVAVALSPIIPNLSLKILQQMGFPL
QDVAQLRWDPDARWGRLPPGQEPQPPVPVFQRILK

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2A subfamily [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=500, Percent_Identity=38.2, Blast_Score=335, Evalue=8e-92,
Organism=Homo sapiens, GI14043022, Length=555, Percent_Identity=26.1261261261261, Blast_Score=162, Evalue=6e-40,
Organism=Escherichia coli, GI1788432, Length=498, Percent_Identity=26.7068273092369, Blast_Score=204, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI212645096, Length=516, Percent_Identity=33.5271317829457, Blast_Score=295, Evalue=4e-80,
Organism=Caenorhabditis elegans, GI71992781, Length=406, Percent_Identity=30.7881773399015, Blast_Score=203, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI17540794, Length=532, Percent_Identity=27.0676691729323, Blast_Score=157, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6321610, Length=515, Percent_Identity=35.1456310679612, Blast_Score=313, Evalue=4e-86,
Organism=Saccharomyces cerevisiae, GI6321704, Length=539, Percent_Identity=25.974025974026, Blast_Score=123, Evalue=6e-29,
Organism=Drosophila melanogaster, GI21358483, Length=549, Percent_Identity=36.0655737704918, Blast_Score=332, Evalue=5e-91,
Organism=Drosophila melanogaster, GI24655311, Length=559, Percent_Identity=26.4758497316637, Blast_Score=148, Evalue=9e-36,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 59136; Mature: 59005

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNSTFVITTPIYYVNAAPHIGSAYTTLAADAIARYHRLRGKDVLMVTGTDEHGQKIQRT
CCCCCEEEECCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHH
AEERGLPPQQHCDEIAAQFAQLWQLLDIRYDRFIRTTDPRHKAIVREFFQRVWDRGDIFV
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEE
GRQQGWYCVACEEFKDESELLEGGFCPLHPNKKVEWKDEENYFFRLSRYQEKLEALYAEH
ECCCCCEEEEHHHHCCHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
PEFIQPESRRNEVINFVKQGLRDFSISRPSVSWGIPFPADPSQTLYVWFDALINYLSAAV
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHC
DPEDEPTLENALRRYWPADVHLVGKDIVRFHAIYWPAMLMSAGLPLPRQIFGHGFLTKDG
CCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCHHH
LKMGKSLGNTLDPFELVQRYGADAVRYYFLTEIELGKDGDFSEGRFIEVLNADLANDLGN
HHHHHHHCCCCCHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCCCEEHHHHHHHHHHHHH
LLNRTLNMLKKYQPDYRIPAVEIPADHPLKTLAERLAPTVADAYERLDPSSAARKALSLA
HHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
QASNKYLDEQAPWTLHKQGQSEQVAQILYAVLESVRWVAVALSPIIPNLSLKILQQMGFP
HHHHHHHCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCC
LQDVAQLRWDPDARWGRLPPGQEPQPPVPVFQRILK
HHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHC
>Mature Secondary Structure 
PNSTFVITTPIYYVNAAPHIGSAYTTLAADAIARYHRLRGKDVLMVTGTDEHGQKIQRT
CCCCEEEECCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHH
AEERGLPPQQHCDEIAAQFAQLWQLLDIRYDRFIRTTDPRHKAIVREFFQRVWDRGDIFV
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEE
GRQQGWYCVACEEFKDESELLEGGFCPLHPNKKVEWKDEENYFFRLSRYQEKLEALYAEH
ECCCCCEEEEHHHHCCHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
PEFIQPESRRNEVINFVKQGLRDFSISRPSVSWGIPFPADPSQTLYVWFDALINYLSAAV
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHC
DPEDEPTLENALRRYWPADVHLVGKDIVRFHAIYWPAMLMSAGLPLPRQIFGHGFLTKDG
CCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCHHH
LKMGKSLGNTLDPFELVQRYGADAVRYYFLTEIELGKDGDFSEGRFIEVLNADLANDLGN
HHHHHHHCCCCCHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCCCEEHHHHHHHHHHHHH
LLNRTLNMLKKYQPDYRIPAVEIPADHPLKTLAERLAPTVADAYERLDPSSAARKALSLA
HHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
QASNKYLDEQAPWTLHKQGQSEQVAQILYAVLESVRWVAVALSPIIPNLSLKILQQMGFP
HHHHHHHCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCC
LQDVAQLRWDPDARWGRLPPGQEPQPPVPVFQRILK
HHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]