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The map label for this gene is rpfG [H]

Identifier: 86605935

GI number: 86605935

Start: 1264886

End: 1266106

Strand: Reverse

Name: rpfG [H]

Synonym: CYA_1252

Alternate gene names: 86605935

Gene position: 1266106-1264886 (Counterclockwise)

Preceding gene: 86605937

Following gene: 86605934

Centisome position: 43.17

GC content: 59.3

Gene sequence:

>1221_bases
ATGTCCCATGGGCCTTTCGCCTTCGATCTCTCTCCTGGCGAAGCCAAATTCGAGGCTGCTGCTGCCTTGCGCCTGAGTCA
GCGGCTGATCCAAGTGGGGATCGCCCTTTCTTCGGAAACGGATTTAAGGCGCCTGCTGGAACTGATTGTCGCCCAAGCCC
GCGAGCTAACCTGCTGTGATGGCGCTAGCCTGTTCATCCGGGAGGGAGAAGAGTTGCGCTTTTTCGTGGTCAACCGCAAC
GAGGAGCTGCAGGATCTGCGCCTGCCCCTTGCTCCTTCCAGCATTGTGGGCTATGTGGTGCTCACGGGGGAGAGCTTGAA
TCTGCCGGACGTCTATCGCTTGCCAGCGGGCTTGCCCTATGCCTTCAACTCCCAAGTCGATCAGCAGACCGGCTACCGCA
CCCGCTCTTTGCTCACGGTGCCGATGCGGGATCCCTCAGGAAACATCCTCGGTGCCCTGCAACTGTTGAACCGCCTCAGG
CCGGATCGCCCTGCTGACCTGGCGCCCGACCAGGTGGCTGACTGGAGCCAGCCGTTTTCTGAGCTGGAAGTCCAAGTGGC
CGAAGCCCTGGCTTCCCAGGCGGCGGTTGCCTACCAAAATGTGCGCTTGCGGGAAGAGCTGAAAGCTGCTCACCTGGAAA
CGATTTTCTGTCTGGCCCAGGCTGCCGAATACCGGGATCAAGACACCGCCTTTCACCTCAAGCGGATGAGCCATTATTCC
CGCATTCTCGCCAAAGAGTTGGGCCTCTCCAGCCATGAACAGGAGCTGATTTTCTACGCCAGCCCCCTGCACGATGTGGG
CAAAATCGGGATCCCGGATGCCATCTTGCAAAAGCCAGGCCCCCTCACCCCCGAAGAGCGCCTGATTATGAACCGCCACC
CCGAGATTGGCTACGAGATCCTCAGTAAATCGGACTCGGAGCTGATGAAAAAAAGCGCCATCATTGCCCTCACCCACCAC
GAGAAGTTCGACGGCAGCGGCTACCCGCGGGGCCTTAAGGGAGAAGAGATCCCGCTGGAAGGGCGGATCGTGGCTTTGGC
CGATGTCTTCGACGCTTTGGCCAGCCGCCGCCCCTACAAAAAAGCTTGGAACCTAGACGAGATCTTTGGCTTTGTGGAGA
GCCAAAGCAGTAGCCACTTCGATCCCCACGTAGTGGCCGCTTTTACCCGTGGCCGCGGCGAAATTCTAGAGATCTACCAC
CGCTACCAAGAGCCTCGCTGA

Upstream 100 bases:

>100_bases
GCGACCCCCAGCCTCCCAACTTGGAGCAGGGGCAGGATCCCTGCCAGAGCTCTAACCCCGCTTCCCTGAGAACACTCGTA
ACCCACCGGAGGATCAGGTT

Downstream 100 bases:

>100_bases
GCTCAGAGCCGGCCAAATCGCGGATTTGGAGTATCCCCTCTTGCCGGGATCCCCACTCGGCAACGGGAAAAACCCGCCTT
TGTCTTTTTTTTCAACCGAT

Product: GAF domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MSHGPFAFDLSPGEAKFEAAAALRLSQRLIQVGIALSSETDLRRLLELIVAQARELTCCDGASLFIREGEELRFFVVNRN
EELQDLRLPLAPSSIVGYVVLTGESLNLPDVYRLPAGLPYAFNSQVDQQTGYRTRSLLTVPMRDPSGNILGALQLLNRLR
PDRPADLAPDQVADWSQPFSELEVQVAEALASQAAVAYQNVRLREELKAAHLETIFCLAQAAEYRDQDTAFHLKRMSHYS
RILAKELGLSSHEQELIFYASPLHDVGKIGIPDAILQKPGPLTPEERLIMNRHPEIGYEILSKSDSELMKKSAIIALTHH
EKFDGSGYPRGLKGEEIPLEGRIVALADVFDALASRRPYKKAWNLDEIFGFVESQSSSHFDPHVVAAFTRGRGEILEIYH
RYQEPR

Sequences:

>Translated_406_residues
MSHGPFAFDLSPGEAKFEAAAALRLSQRLIQVGIALSSETDLRRLLELIVAQARELTCCDGASLFIREGEELRFFVVNRN
EELQDLRLPLAPSSIVGYVVLTGESLNLPDVYRLPAGLPYAFNSQVDQQTGYRTRSLLTVPMRDPSGNILGALQLLNRLR
PDRPADLAPDQVADWSQPFSELEVQVAEALASQAAVAYQNVRLREELKAAHLETIFCLAQAAEYRDQDTAFHLKRMSHYS
RILAKELGLSSHEQELIFYASPLHDVGKIGIPDAILQKPGPLTPEERLIMNRHPEIGYEILSKSDSELMKKSAIIALTHH
EKFDGSGYPRGLKGEEIPLEGRIVALADVFDALASRRPYKKAWNLDEIFGFVESQSSSHFDPHVVAAFTRGRGEILEIYH
RYQEPR
>Mature_405_residues
SHGPFAFDLSPGEAKFEAAAALRLSQRLIQVGIALSSETDLRRLLELIVAQARELTCCDGASLFIREGEELRFFVVNRNE
ELQDLRLPLAPSSIVGYVVLTGESLNLPDVYRLPAGLPYAFNSQVDQQTGYRTRSLLTVPMRDPSGNILGALQLLNRLRP
DRPADLAPDQVADWSQPFSELEVQVAEALASQAAVAYQNVRLREELKAAHLETIFCLAQAAEYRDQDTAFHLKRMSHYSR
ILAKELGLSSHEQELIFYASPLHDVGKIGIPDAILQKPGPLTPEERLIMNRHPEIGYEILSKSDSELMKKSAIIALTHHE
KFDGSGYPRGLKGEEIPLEGRIVALADVFDALASRRPYKKAWNLDEIFGFVESQSSSHFDPHVVAAFTRGRGEILEIYHR
YQEPR

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Homo sapiens, GI226246591, Length=139, Percent_Identity=32.3741007194245, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI219802706, Length=139, Percent_Identity=32.3741007194245, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI4505657, Length=139, Percent_Identity=32.3741007194245, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI116536085, Length=173, Percent_Identity=28.3236994219653, Blast_Score=70, Evalue=5e-12,
Organism=Drosophila melanogaster, GI161076955, Length=188, Percent_Identity=25, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI161076953, Length=188, Percent_Identity=25, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 45438; Mature: 45307

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHGPFAFDLSPGEAKFEAAAALRLSQRLIQVGIALSSETDLRRLLELIVAQARELTCCD
CCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCEEECC
GASLFIREGEELRFFVVNRNEELQDLRLPLAPSSIVGYVVLTGESLNLPDVYRLPAGLPY
CCEEEEECCCEEEEEEECCCCCHHHHCCCCCCCCCEEEEEEECCCCCCCHHHHCCCCCCC
AFNSQVDQQTGYRTRSLLTVPMRDPSGNILGALQLLNRLRPDRPADLAPDQVADWSQPFS
HHCCCHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCHH
ELEVQVAEALASQAAVAYQNVRLREELKAAHLETIFCLAQAAEYRDQDTAFHLKRMSHYS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
RILAKELGLSSHEQELIFYASPLHDVGKIGIPDAILQKPGPLTPEERLIMNRHPEIGYEI
HHHHHHHCCCCCCCEEEEEECCHHHHCCCCCCHHHHCCCCCCCHHHHHHHCCCCCHHHHH
LSKSDSELMKKSAIIALTHHEKFDGSGYPRGLKGEEIPLEGRIVALADVFDALASRRPYK
HCCCHHHHHHHCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHCCCCCH
KAWNLDEIFGFVESQSSSHFDPHVVAAFTRGRGEILEIYHRYQEPR
HHCCHHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
SHGPFAFDLSPGEAKFEAAAALRLSQRLIQVGIALSSETDLRRLLELIVAQARELTCCD
CCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCEEECC
GASLFIREGEELRFFVVNRNEELQDLRLPLAPSSIVGYVVLTGESLNLPDVYRLPAGLPY
CCEEEEECCCEEEEEEECCCCCHHHHCCCCCCCCCEEEEEEECCCCCCCHHHHCCCCCCC
AFNSQVDQQTGYRTRSLLTVPMRDPSGNILGALQLLNRLRPDRPADLAPDQVADWSQPFS
HHCCCHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCHH
ELEVQVAEALASQAAVAYQNVRLREELKAAHLETIFCLAQAAEYRDQDTAFHLKRMSHYS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
RILAKELGLSSHEQELIFYASPLHDVGKIGIPDAILQKPGPLTPEERLIMNRHPEIGYEI
HHHHHHHCCCCCCCEEEEEECCHHHHCCCCCCHHHHCCCCCCCHHHHHHHCCCCCHHHHH
LSKSDSELMKKSAIIALTHHEKFDGSGYPRGLKGEEIPLEGRIVALADVFDALASRRPYK
HCCCHHHHHHHCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHCCCCCH
KAWNLDEIFGFVESQSSSHFDPHVVAAFTRGRGEILEIYHRYQEPR
HHCCHHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]