Click here to switch to the map view.

The map label for this gene is pilJ [H]

Identifier: 86605934

GI number: 86605934

Start: 1263499

End: 1264620

Strand: Reverse

Name: pilJ [H]

Synonym: CYA_1250

Alternate gene names: 86605934

Gene position: 1264620-1263499 (Counterclockwise)

Preceding gene: 86605935

Following gene: 86605928

Centisome position: 43.12

GC content: 61.05

Gene sequence:

>1122_bases
ATGGCTACCCTGACCAATCCGCCCACTCCCACCCTCTCGATCCACACCAAGCTGCGCGATCTGGCGCTAGTGGACTTTCA
GGTAGGTGAGTCCACCCCCTGCGAGAACGTGGTGCGCCGCCTAGAAGACGACCCCACCCTTTCAGGGGTAGTCGTGTTGG
ACGAGCAGTCGCAGGTGCGGGGGATGCTGACTCGGCGTGCCATTTTGGAGTGGATGCTGAGCAAACCCTATGGCCTGGAG
GTGTTTTCGAAGCGTCCCATCAGCTCCCTAGCAGAGTTCCAGGGCCAGGGTTTTCTCCTTTTGCCCGGCGACTGCAATGT
CATTGAGGCGGCCAGCCAGGCTTTCCGGCGGCCCCAGGAGACTATCTACGACCCGGTGGTGGTAGAAGTCGGCCCCCAAG
ACTATCGCCTTTTGGATGTGCCGGTGTTGTTGGTGGCTCAGTCCCAGGTACATCTAGCCACCCAGCGGAAGCTCGAAGAA
CAGCAGGAGGAGATGAAACGGCTCTTGGCCGAGCTGGAGCAGGAAAAAAACCGCAGCCTTCAGTATGCCCGCGATCTGGA
GCGGCAAAAAGCCGAGATCCTCAGCCAAAACCTGGCTTTGGATCGGGAGCGGCGCCAAGCCCAGCAGCGCTCGGAAGAGC
TGGCCCGCCTCAACGCCCGCATCATCGAGATCAGCTCGGTGCTCTCGGAAAAGGGCAAATCCACCTTTGCCGCCACCTTT
GCCGGGGTAGAGGCGGTGCGCCGCCTATTTCAGGACATTGCCGACAGCAACCGGGAGCTCTCCCGCGAGCTCAAAGAAAT
CAACACCATCGTTGACTTGATCGTAGAGGTGGCAGGCTATATTCGCCTGCTCTCCTTTAATGCGGCTGTGGAGGCCAACC
GCAGGGGTGGGGGCGGCGGTTTTGGTGCCATTGCCCAGGAGATCCGCAAATTGGCGGGGCGCACCACCGAAGCCAGCAAC
CGCATCCGCGGCTTGGCAGAGCGCATCCAACGCCAAAGCCAATCCACCCTGCAATCGGCTCAGGCCAGCGCCGAGATGGT
GCAATCCCTTTACCAGCAGGCGCAGTCGGCCCAGGCAGCCCTGGAGGAATTGCAAGCGCTGTTGGAGCAGGCCCAGGTTT
AG

Upstream 100 bases:

>100_bases
CTCCTGGCAAGCCGACCAGAGCCACTTTTGTGTGTTTCCCAATACATAGCACATCTACTGGCTACCTGTCTCGACCCAAG
CTGCGCCGAGGATCCCTGTG

Downstream 100 bases:

>100_bases
CCCTTTTGGGGAGTGGTTTTGCCCTCGCGGATCTCATAGGTATAGAATTGCCCTGCCACGGCCAAGGTTTCAAAGTGGTA
GGTGGGCAGGGAGATCCCCA

Product: methyl-accepting chemotaxis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 373; Mature: 372

Protein sequence:

>373_residues
MATLTNPPTPTLSIHTKLRDLALVDFQVGESTPCENVVRRLEDDPTLSGVVVLDEQSQVRGMLTRRAILEWMLSKPYGLE
VFSKRPISSLAEFQGQGFLLLPGDCNVIEAASQAFRRPQETIYDPVVVEVGPQDYRLLDVPVLLVAQSQVHLATQRKLEE
QQEEMKRLLAELEQEKNRSLQYARDLERQKAEILSQNLALDRERRQAQQRSEELARLNARIIEISSVLSEKGKSTFAATF
AGVEAVRRLFQDIADSNRELSRELKEINTIVDLIVEVAGYIRLLSFNAAVEANRRGGGGGFGAIAQEIRKLAGRTTEASN
RIRGLAERIQRQSQSTLQSAQASAEMVQSLYQQAQSAQAALEELQALLEQAQV

Sequences:

>Translated_373_residues
MATLTNPPTPTLSIHTKLRDLALVDFQVGESTPCENVVRRLEDDPTLSGVVVLDEQSQVRGMLTRRAILEWMLSKPYGLE
VFSKRPISSLAEFQGQGFLLLPGDCNVIEAASQAFRRPQETIYDPVVVEVGPQDYRLLDVPVLLVAQSQVHLATQRKLEE
QQEEMKRLLAELEQEKNRSLQYARDLERQKAEILSQNLALDRERRQAQQRSEELARLNARIIEISSVLSEKGKSTFAATF
AGVEAVRRLFQDIADSNRELSRELKEINTIVDLIVEVAGYIRLLSFNAAVEANRRGGGGGFGAIAQEIRKLAGRTTEASN
RIRGLAERIQRQSQSTLQSAQASAEMVQSLYQQAQSAQAALEELQALLEQAQV
>Mature_372_residues
ATLTNPPTPTLSIHTKLRDLALVDFQVGESTPCENVVRRLEDDPTLSGVVVLDEQSQVRGMLTRRAILEWMLSKPYGLEV
FSKRPISSLAEFQGQGFLLLPGDCNVIEAASQAFRRPQETIYDPVVVEVGPQDYRLLDVPVLLVAQSQVHLATQRKLEEQ
QEEMKRLLAELEQEKNRSLQYARDLERQKAEILSQNLALDRERRQAQQRSEELARLNARIIEISSVLSEKGKSTFAATFA
GVEAVRRLFQDIADSNRELSRELKEINTIVDLIVEVAGYIRLLSFNAAVEANRRGGGGGFGAIAQEIRKLAGRTTEASNR
IRGLAERIQRQSQSTLQSAQASAEMVQSLYQQAQSAQAALEELQALLEQAQV

Specific function: May be a part of a signal-transduction system that regulates twitching motility by controlling pilus function (extension and retraction) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 methyl-accepting transducer domain [H]

Homologues:

Organism=Escherichia coli, GI1789453, Length=166, Percent_Identity=33.1325301204819, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004090
- InterPro:   IPR004089
- InterPro:   IPR003660 [H]

Pfam domain/function: PF00015 MCPsignal [H]

EC number: NA

Molecular weight: Translated: 41663; Mature: 41532

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS50111 CHEMOTAXIS_TRANSDUC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATLTNPPTPTLSIHTKLRDLALVDFQVGESTPCENVVRRLEDDPTLSGVVVLDEQSQVR
CCCCCCCCCCCEEHHHHHHHHHHEEEECCCCCCHHHHHHHHCCCCCCCCEEEECCHHHHH
GMLTRRAILEWMLSKPYGLEVFSKRPISSLAEFQGQGFLLLPGDCNVIEAASQAFRRPQE
HHHHHHHHHHHHHCCCCCCHHHCCCCHHHHHHHCCCCEEEECCCCCHHHHHHHHHHCCHH
TIYDPVVVEVGPQDYRLLDVPVLLVAQSQVHLATQRKLEEQQEEMKRLLAELEQEKNRSL
HHCCCEEEEECCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QYARDLERQKAEILSQNLALDRERRQAQQRSEELARLNARIIEISSVLSEKGKSTFAATF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
AGVEAVRRLFQDIADSNRELSRELKEINTIVDLIVEVAGYIRLLSFNAAVEANRRGGGGG
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
FGAIAQEIRKLAGRTTEASNRIRGLAERIQRQSQSTLQSAQASAEMVQSLYQQAQSAQAA
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEELQALLEQAQV
HHHHHHHHHHHCC
>Mature Secondary Structure 
ATLTNPPTPTLSIHTKLRDLALVDFQVGESTPCENVVRRLEDDPTLSGVVVLDEQSQVR
CCCCCCCCCCEEHHHHHHHHHHEEEECCCCCCHHHHHHHHCCCCCCCCEEEECCHHHHH
GMLTRRAILEWMLSKPYGLEVFSKRPISSLAEFQGQGFLLLPGDCNVIEAASQAFRRPQE
HHHHHHHHHHHHHCCCCCCHHHCCCCHHHHHHHCCCCEEEECCCCCHHHHHHHHHHCCHH
TIYDPVVVEVGPQDYRLLDVPVLLVAQSQVHLATQRKLEEQQEEMKRLLAELEQEKNRSL
HHCCCEEEEECCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QYARDLERQKAEILSQNLALDRERRQAQQRSEELARLNARIIEISSVLSEKGKSTFAATF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
AGVEAVRRLFQDIADSNRELSRELKEINTIVDLIVEVAGYIRLLSFNAAVEANRRGGGGG
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
FGAIAQEIRKLAGRTTEASNRIRGLAERIQRQSQSTLQSAQASAEMVQSLYQQAQSAQAA
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEELQALLEQAQV
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7908398; 10984043 [H]