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The map label for this gene is pyk [H]

Identifier: 86605894

GI number: 86605894

Start: 1219396

End: 1220859

Strand: Reverse

Name: pyk [H]

Synonym: CYA_1210

Alternate gene names: 86605894

Gene position: 1220859-1219396 (Counterclockwise)

Preceding gene: 86605896

Following gene: 86605892

Centisome position: 41.63

GC content: 63.66

Gene sequence:

>1464_bases
ATGGCTTCCTCTTCCCCCTCTTCGCCTAAGCGTTTGCTAGAGCAGCTCCTGGAGCTGCGACAGCAAGTTGTGGAGGGATC
CCAGAAGTACTTGACCGCCTGGGGCAGCCCCTGCAACCCCTCCCCCGCTCTCGAGAACCTAGCTCGGTATTTGGCGCTAC
GCCAACACGATCTGCGCAACCTGCAATTGGAGCTGGCTGCGGTGGGGCTGTCCTCGCTGGGACGGTGTGAATCGCGGGTC
TTAGAGACCCTGGATGCAGTGATCGCCACTTTGGCAGCCACGCAGGGCCTAAGCCACAAGGGGCCCAGCTATGAGGAGTT
TTACGCTGGGGACATGGCGCTGGAGCAGCATGCTGAGGCGCTGTTTGGCCCTCCTTCTCCCCACCGCCGCGCCCGCATCA
TGGTGACCCTGCCCAGCGAAGCTGCCGAGCAGCCCGAGCTGCTGCTGCAACTGCTGCAACGGGGGATGAACGTGGCCCGG
ATCAACTGCGCCCACGACGATCCCCTGGTGTGGGAGAAAATGGTGGCCCACTTGCGCCAGGCCGAAGCCCAGACTCAACG
GCGCTGCAAGATTTTGTTCGACCTGGCCGGCCCCAAGATCCGCACGGGCCTGGTGGCCATGCCACCCGGCAAAGCCAAGG
TGTACGGTGGGGATCGCATCCTGCTCACGGCAGCCATTCCGCCGGTCGACTCTGACATCTGCTGCCAGGTGGCTTGCACC
CTGCCGGAGATCTTGGCCCACCTCAAGGTGGGGGCAACTGTTTGGATCGACGACGGCAAAATCGGCGCTCGTGTAGTGGG
GATCGAGCCGGCAGGGGTGGTGCTGGAGGTGGACAAAGTCGCCCCGCAGGGCAAGAAGCTGCGGGAGGAAAAAGGCCTCA
ACTTTCCCGACAGCCAGCTCTCGATCCAGCCCCTCACCGAGAAAGATCGCCAAGACTTGGACTTCGTGGTGCGGCATGCC
GACCTGGTGGGCTATTCCTTTGTCCAGCAGCCGGCGGATTTGCAGTTGCTGTTTTCTGAGCTGGAGCGGCGCCAGGCCCG
GCCCGATCTGGGCTTGATCCTCAAGATTGAAACGCAGCGAGCGGTGCAAAACCTGCCCACTCTGATCGCGGCCGTCGACG
GCTGCCGGCCCCTGGGGGTGATGATTGCCCGGGGAGATCTGGCCGTGGAGATTGGCTGGCTGCGCCTGGGCGAGATCCAG
GAGGAGCTGCTGTGGATCTGTGAGGCCGCCCATGTGCCGGTAGTCTGGGCTACGCAGGTGCTGGATCAGCTCACCAAGGA
GGGCCTGCCCTCCCGGCCTGAGCTTTCCGATGCGGTGCTGTCTGCCCGCGCCGAGTGCGTCATGCTCAATAAGGGCCCCT
ATCTCCTGGAGGCGGTTGCCCTGCTGGACGAGCTGCTGGCCCGCATGCAGGCCCACCAGCACAAAAAAAGCCAACGCCTG
CGAGCTTTGTACTCCTGGCGATGA

Upstream 100 bases:

>100_bases
AGGTTAATCGCCTCCCTTTTTCACCCTAACTTGCCTATGATCGACCCAAGGCGGGCTTGGGCCGGGCGTTGGGCTTGTTT
GTCTTGAGATAGCTTTCAGC

Downstream 100 bases:

>100_bases
GATGGAGCGGCATCAAAGACGGAACTTCTCCCCCAGGTAATACTTGCGCACCAAGGGATCCGCCGCCAGCTCCGCTGGTG
TGCCCTTGGCCAGGATGGCG

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 487; Mature: 486

Protein sequence:

>487_residues
MASSSPSSPKRLLEQLLELRQQVVEGSQKYLTAWGSPCNPSPALENLARYLALRQHDLRNLQLELAAVGLSSLGRCESRV
LETLDAVIATLAATQGLSHKGPSYEEFYAGDMALEQHAEALFGPPSPHRRARIMVTLPSEAAEQPELLLQLLQRGMNVAR
INCAHDDPLVWEKMVAHLRQAEAQTQRRCKILFDLAGPKIRTGLVAMPPGKAKVYGGDRILLTAAIPPVDSDICCQVACT
LPEILAHLKVGATVWIDDGKIGARVVGIEPAGVVLEVDKVAPQGKKLREEKGLNFPDSQLSIQPLTEKDRQDLDFVVRHA
DLVGYSFVQQPADLQLLFSELERRQARPDLGLILKIETQRAVQNLPTLIAAVDGCRPLGVMIARGDLAVEIGWLRLGEIQ
EELLWICEAAHVPVVWATQVLDQLTKEGLPSRPELSDAVLSARAECVMLNKGPYLLEAVALLDELLARMQAHQHKKSQRL
RALYSWR

Sequences:

>Translated_487_residues
MASSSPSSPKRLLEQLLELRQQVVEGSQKYLTAWGSPCNPSPALENLARYLALRQHDLRNLQLELAAVGLSSLGRCESRV
LETLDAVIATLAATQGLSHKGPSYEEFYAGDMALEQHAEALFGPPSPHRRARIMVTLPSEAAEQPELLLQLLQRGMNVAR
INCAHDDPLVWEKMVAHLRQAEAQTQRRCKILFDLAGPKIRTGLVAMPPGKAKVYGGDRILLTAAIPPVDSDICCQVACT
LPEILAHLKVGATVWIDDGKIGARVVGIEPAGVVLEVDKVAPQGKKLREEKGLNFPDSQLSIQPLTEKDRQDLDFVVRHA
DLVGYSFVQQPADLQLLFSELERRQARPDLGLILKIETQRAVQNLPTLIAAVDGCRPLGVMIARGDLAVEIGWLRLGEIQ
EELLWICEAAHVPVVWATQVLDQLTKEGLPSRPELSDAVLSARAECVMLNKGPYLLEAVALLDELLARMQAHQHKKSQRL
RALYSWR
>Mature_486_residues
ASSSPSSPKRLLEQLLELRQQVVEGSQKYLTAWGSPCNPSPALENLARYLALRQHDLRNLQLELAAVGLSSLGRCESRVL
ETLDAVIATLAATQGLSHKGPSYEEFYAGDMALEQHAEALFGPPSPHRRARIMVTLPSEAAEQPELLLQLLQRGMNVARI
NCAHDDPLVWEKMVAHLRQAEAQTQRRCKILFDLAGPKIRTGLVAMPPGKAKVYGGDRILLTAAIPPVDSDICCQVACTL
PEILAHLKVGATVWIDDGKIGARVVGIEPAGVVLEVDKVAPQGKKLREEKGLNFPDSQLSIQPLTEKDRQDLDFVVRHAD
LVGYSFVQQPADLQLLFSELERRQARPDLGLILKIETQRAVQNLPTLIAAVDGCRPLGVMIARGDLAVEIGWLRLGEIQE
ELLWICEAAHVPVVWATQVLDQLTKEGLPSRPELSDAVLSARAECVMLNKGPYLLEAVALLDELLARMQAHQHKKSQRLR
ALYSWR

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PEP- utilizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI32967597, Length=352, Percent_Identity=31.25, Blast_Score=142, Evalue=6e-34,
Organism=Homo sapiens, GI10835121, Length=352, Percent_Identity=31.25, Blast_Score=142, Evalue=7e-34,
Organism=Homo sapiens, GI33286422, Length=364, Percent_Identity=31.3186813186813, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI33286420, Length=364, Percent_Identity=31.3186813186813, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI33286418, Length=364, Percent_Identity=31.3186813186813, Blast_Score=134, Evalue=3e-31,
Organism=Homo sapiens, GI310128732, Length=308, Percent_Identity=28.8961038961039, Blast_Score=102, Evalue=9e-22,
Organism=Homo sapiens, GI310128730, Length=308, Percent_Identity=28.8961038961039, Blast_Score=102, Evalue=9e-22,
Organism=Homo sapiens, GI310128736, Length=252, Percent_Identity=28.1746031746032, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI310128734, Length=252, Percent_Identity=28.1746031746032, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI310128738, Length=225, Percent_Identity=28, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1787965, Length=354, Percent_Identity=32.7683615819209, Blast_Score=151, Evalue=9e-38,
Organism=Escherichia coli, GI1788160, Length=349, Percent_Identity=30.945558739255, Blast_Score=108, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17544584, Length=356, Percent_Identity=29.2134831460674, Blast_Score=131, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI17506829, Length=417, Percent_Identity=29.4964028776978, Blast_Score=131, Evalue=9e-31,
Organism=Caenorhabditis elegans, GI71984413, Length=417, Percent_Identity=29.4964028776978, Blast_Score=131, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17506831, Length=359, Percent_Identity=31.1977715877437, Blast_Score=128, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI71984406, Length=359, Percent_Identity=31.1977715877437, Blast_Score=128, Evalue=9e-30,
Organism=Saccharomyces cerevisiae, GI6319279, Length=357, Percent_Identity=30.5322128851541, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6324923, Length=366, Percent_Identity=28.9617486338798, Blast_Score=124, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24648964, Length=383, Percent_Identity=31.5926892950392, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI28571814, Length=383, Percent_Identity=31.5926892950392, Blast_Score=149, Evalue=4e-36,
Organism=Drosophila melanogaster, GI24648966, Length=335, Percent_Identity=28.955223880597, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24581235, Length=370, Percent_Identity=23.7837837837838, Blast_Score=103, Evalue=4e-22,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 53710; Mature: 53579

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASSSPSSPKRLLEQLLELRQQVVEGSQKYLTAWGSPCNPSPALENLARYLALRQHDLRN
CCCCCCCCHHHHHHHHHHHHHHHHCCCHHEEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
LQLELAAVGLSSLGRCESRVLETLDAVIATLAATQGLSHKGPSYEEFYAGDMALEQHAEA
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHHHH
LFGPPSPHRRARIMVTLPSEAAEQPELLLQLLQRGMNVARINCAHDDPLVWEKMVAHLRQ
HCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHH
AEAQTQRRCKILFDLAGPKIRTGLVAMPPGKAKVYGGDRILLTAAIPPVDSDICCQVACT
HHHHHHHHHEEEEECCCCCHHCCEEECCCCCEEEECCCEEEEEEECCCCCCHHHHHHHHH
LPEILAHLKVGATVWIDDGKIGARVVGIEPAGVVLEVDKVAPQGKKLREEKGLNFPDSQL
HHHHHHHHHCCCEEEEECCCCCEEEEEECCCCEEEEECCCCCCCHHHHHHCCCCCCCCCC
SIQPLTEKDRQDLDFVVRHADLVGYSFVQQPADLQLLFSELERRQARPDLGLILKIETQR
EECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEEEEHHH
AVQNLPTLIAAVDGCRPLGVMIARGDLAVEIGWLRLGEIQEELLWICEAAHVPVVWATQV
HHHHHHHHHHHHCCCCCCEEEEECCCEEEEECCEEHHHHHHHHHHHHHHCCCCHHHHHHH
LDQLTKEGLPSRPELSDAVLSARAECVMLNKGPYLLEAVALLDELLARMQAHQHKKSQRL
HHHHHHCCCCCCCCHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RALYSWR
HHHHCCC
>Mature Secondary Structure 
ASSSPSSPKRLLEQLLELRQQVVEGSQKYLTAWGSPCNPSPALENLARYLALRQHDLRN
CCCCCCCHHHHHHHHHHHHHHHHCCCHHEEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
LQLELAAVGLSSLGRCESRVLETLDAVIATLAATQGLSHKGPSYEEFYAGDMALEQHAEA
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHHHH
LFGPPSPHRRARIMVTLPSEAAEQPELLLQLLQRGMNVARINCAHDDPLVWEKMVAHLRQ
HCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHH
AEAQTQRRCKILFDLAGPKIRTGLVAMPPGKAKVYGGDRILLTAAIPPVDSDICCQVACT
HHHHHHHHHEEEEECCCCCHHCCEEECCCCCEEEECCCEEEEEEECCCCCCHHHHHHHHH
LPEILAHLKVGATVWIDDGKIGARVVGIEPAGVVLEVDKVAPQGKKLREEKGLNFPDSQL
HHHHHHHHHCCCEEEEECCCCCEEEEEECCCCEEEEECCCCCCCHHHHHHCCCCCCCCCC
SIQPLTEKDRQDLDFVVRHADLVGYSFVQQPADLQLLFSELERRQARPDLGLILKIETQR
EECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEEEEHHH
AVQNLPTLIAAVDGCRPLGVMIARGDLAVEIGWLRLGEIQEELLWICEAAHVPVVWATQV
HHHHHHHHHHHHCCCCCCEEEEECCCEEEEECCEEHHHHHHHHHHHHHHCCCCHHHHHHH
LDQLTKEGLPSRPELSDAVLSARAECVMLNKGPYLLEAVALLDELLARMQAHQHKKSQRL
HHHHHHCCCCCCCCHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RALYSWR
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7549104 [H]