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The map label for this gene is trmH [C]

Identifier: 86605783

GI number: 86605783

Start: 1114734

End: 1115546

Strand: Reverse

Name: trmH [C]

Synonym: CYA_1093

Alternate gene names: 86605783

Gene position: 1115546-1114734 (Counterclockwise)

Preceding gene: 86605784

Following gene: 86605781

Centisome position: 38.04

GC content: 64.33

Gene sequence:

>813_bases
ATGGCTTCTGAGCTGCTCACCAGCCCCAAAAACCCTTGGATCCGACAGTTGCGGCAGTTGCACCGCAGCAAAGGCCGGCG
AGAACAGGGCGCCTTCCTGGTCGAGGGATCCCACCTCCTCGAAGAGGCGCTGGGGGTCGGCTGGCCTCTGCGAGCTGTCT
GCTACACCTTCGACTGGGCTGAGCGTCACCCCCGCTTGTTGGCCAGCCTGCCCAAGCATCTGCGGCGGCAGGCCGTCAGC
TTGGAAGTGATGGCGGCCCTCTCTACGACCGAGTCTCCGGAAGGGGTTGTAGCTGTGGCCGTTAGCCGGCAAATCGCCTC
CCCTCCCCCAGAGGCGCTGCGCCTGGGCTTGGTGGTCGAAACGCTGCAAGATCCAGGTAATTTGGGATCCCTGATCCGCG
TCGTGGCGGCCACCGGAGCCGATGGCCTCTGGCTAAGCGCAGACAGCGTCGATCCGGAAAACCCCAAAGTCCTGCGGGCC
TCCGCCGGCCAGTGGTTTCGCCGCCCGCCTCAGGTTCTCTCTGACTTGCCCGCCTGGATCCAGTCCTGTCGTCGCCAAGG
GATCCAAGTTTTGGCCGCCACCCTCGCCCCTCTGGCCACCTGTTACTGGGATCACGACCTCACTCGGCCAACCCTTTTTC
TCTTGGGCAACGAAGGGGCTGGCCTGCAGGAATCGACGATCCAACTGGCCGATGCCCAGGTGCGGATCCCGATGGCTGCT
GGCGTCGAGTCCCTCAACGTGGCCATGACCGGGGGATTGCTGCTCTACGAAGCTTTGCGCCAGCAGCGAGCCCAGTTGAG
AGGCACAGCTTGA

Upstream 100 bases:

>100_bases
GCTCATCTGGCCTCATCGCCAGGATCGGGATGGATTTTTTATCCAACGGTTGCGTCGCCGCGATTAAGCCTTCCCCGCCC
CATCTCTCCTCTTCCTCCGC

Downstream 100 bases:

>100_bases
AGAGCCGCCCTCGTTTCAAAACTCCTCATCTGCAAAGATGGGGGCCTCAACCTCTTCCTGCTCTCCCTCCTCTATCCCCC
CCGGTCGGCTGGCAGAAACC

Product: RNA methyltransferase

Products: S-adenosyl-L-homocysteine; tRNATyr Containing 2'-O-methylguanosine [C]

Alternate protein names: NA

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MASELLTSPKNPWIRQLRQLHRSKGRREQGAFLVEGSHLLEEALGVGWPLRAVCYTFDWAERHPRLLASLPKHLRRQAVS
LEVMAALSTTESPEGVVAVAVSRQIASPPPEALRLGLVVETLQDPGNLGSLIRVVAATGADGLWLSADSVDPENPKVLRA
SAGQWFRRPPQVLSDLPAWIQSCRRQGIQVLAATLAPLATCYWDHDLTRPTLFLLGNEGAGLQESTIQLADAQVRIPMAA
GVESLNVAMTGGLLLYEALRQQRAQLRGTA

Sequences:

>Translated_270_residues
MASELLTSPKNPWIRQLRQLHRSKGRREQGAFLVEGSHLLEEALGVGWPLRAVCYTFDWAERHPRLLASLPKHLRRQAVS
LEVMAALSTTESPEGVVAVAVSRQIASPPPEALRLGLVVETLQDPGNLGSLIRVVAATGADGLWLSADSVDPENPKVLRA
SAGQWFRRPPQVLSDLPAWIQSCRRQGIQVLAATLAPLATCYWDHDLTRPTLFLLGNEGAGLQESTIQLADAQVRIPMAA
GVESLNVAMTGGLLLYEALRQQRAQLRGTA
>Mature_269_residues
ASELLTSPKNPWIRQLRQLHRSKGRREQGAFLVEGSHLLEEALGVGWPLRAVCYTFDWAERHPRLLASLPKHLRRQAVSL
EVMAALSTTESPEGVVAVAVSRQIASPPPEALRLGLVVETLQDPGNLGSLIRVVAATGADGLWLSADSVDPENPKVLRAS
AGQWFRRPPQVLSDLPAWIQSCRRQGIQVLAATLAPLATCYWDHDLTRPTLFLLGNEGAGLQESTIQLADAQVRIPMAAG
VESLNVAMTGGLLLYEALRQQRAQLRGTA

Specific function: Specifically Methylates Guanosine-18 In Various tRNAs. [C]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Homo sapiens, GI110825988, Length=154, Percent_Identity=30.5194805194805, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI40068479, Length=178, Percent_Identity=31.4606741573034, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1790083, Length=154, Percent_Identity=32.4675324675325, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.34 [C]

Molecular weight: Translated: 29406; Mature: 29275

Theoretical pI: Translated: 8.50; Mature: 8.50

Prosite motif: PS00012 PHOSPHOPANTETHEINE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASELLTSPKNPWIRQLRQLHRSKGRREQGAFLVEGSHLLEEALGVGWPLRAVCYTFDWA
CCCCHHCCCCCHHHHHHHHHHHHCCCHHCCCEEEECHHHHHHHHCCCCCHHHHHHHHHHH
ERHPRLLASLPKHLRRQAVSLEVMAALSTTESPEGVVAVAVSRQIASPPPEALRLGLVVE
HHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHCCCCHHHHHHHHHHH
TLQDPGNLGSLIRVVAATGADGLWLSADSVDPENPKVLRASAGQWFRRPPQVLSDLPAWI
HHHCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEECCCCHHHCCCHHHHHHHHHHH
QSCRRQGIQVLAATLAPLATCYWDHDLTRPTLFLLGNEGAGLQESTIQLADAQVRIPMAA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHCCEEEEHHHH
GVESLNVAMTGGLLLYEALRQQRAQLRGTA
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ASELLTSPKNPWIRQLRQLHRSKGRREQGAFLVEGSHLLEEALGVGWPLRAVCYTFDWA
CCCHHCCCCCHHHHHHHHHHHHCCCHHCCCEEEECHHHHHHHHCCCCCHHHHHHHHHHH
ERHPRLLASLPKHLRRQAVSLEVMAALSTTESPEGVVAVAVSRQIASPPPEALRLGLVVE
HHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHCCCCHHHHHHHHHHH
TLQDPGNLGSLIRVVAATGADGLWLSADSVDPENPKVLRASAGQWFRRPPQVLSDLPAWI
HHHCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEECCCCHHHCCCHHHHHHHHHHH
QSCRRQGIQVLAATLAPLATCYWDHDLTRPTLFLLGNEGAGLQESTIQLADAQVRIPMAA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHCCEEEEHHHH
GVESLNVAMTGGLLLYEALRQQRAQLRGTA
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; tRNATyr [C]

Specific reaction: S-Adenosyl-L-methionine + tRNATyr = S-adenosyl-L-homocysteine + tRNATyr Containing 2'-O-methylguanosine [C]

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]