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The map label for this gene is 86605763

Identifier: 86605763

GI number: 86605763

Start: 1084726

End: 1086174

Strand: Reverse

Name: 86605763

Synonym: CYA_1068

Alternate gene names: NA

Gene position: 1086174-1084726 (Counterclockwise)

Preceding gene: 86605766

Following gene: 86605762

Centisome position: 37.04

GC content: 62.11

Gene sequence:

>1449_bases
ATGGTCAGCGTCGCTTCCCGCTCCTACAGCACCACCGACTGGCAGTTGGGCTATCGCTCTTTACGCCAGGAGTATGACTA
TTGGGCGACGGAAATCGAGGGGCGGATCCCCGAAAGGCTGCAGGGAACCCTCTTCCGCAACGGGCCTGGCAAGCTGGAGG
CGGGATCCCAGCGCTACGGCCATCCCTTCGACGGCGACGGCATGGTGTGTGCCATCACCTTTATTGACGGGCGGGTGCAT
TTCCGCAACCGGTTTGTGCGCACTCCCGAGTTTGAGGCCGAGGAGAAGGCGGGACGGGTGCTCTACCGCGGCGTATTCGG
CACCCAGAAGCCGGGAGGCTGGTGGGCCAATTTCCTCGATCTGCGCTTCAAAAACCCCGCCAACACCAACGTTATCTACC
AAGGCGGCAAGCTCCTGGCGCTTTGGGAGGCCAGCGCTCCCTATCGGCTCAACCCCGCCACCTTGGCCACCGAAGGGAGC
GAGACCTTTGCCGGTGCCCTTGCCCCCAACCAGCCGTTTACGGCGCATCCCCGCCGGGATCCAGCCACGGGGGATCTCAT
GGCCTTTGGGGTGCAGACAGGACCTTCCTCCACGCTGCTGTTTTACCGCGTCGACGCCCAAGGAAGGCTGGTGGAAAAGC
GGGACTACCGCCTGCCGGGTTTTGCCTTTCTGCACGACTTTGTCTGGACGCCCCGCTACTGGATCTTTTTCCAGAATCCG
GTGGCCTTCAACCCCTTGCCCTATATGCTGGGCCTGCAGCCGGCGGGCACCTGCTTGACCCTAAAAAAAGGAGAGCCCAC
CCGCATTTGGCTATTTCCCCAACAGGGCGAGCCGCTGCAACTGCAAACGGAGCCGGGGTTTGTTTTCCACTTTGTCAACG
GCTACGAGGTTGGGGATCAGGTGGTGGTGGATGCCGTTCTCTACGAAGCCTACCCTGCCCTGGAGCCAGGGGCTGACTAC
TTGCAAATTGACTTTGCCCAGGTGCCAGCCGGCAAGCTGTGGCGCTTTGAGCTGGATCCCAAGGCGGGCAAGGTTAAGCG
GGAGCTACTGCTGGATCGCTCGGTGGAGTTTCCGGCTATCCATCCGGCCCGTATGGGGGGATCCCATCGCTACATCTACA
TTGGGGCTACCCATGCTCCCGGCCCCAACGCCCCCCTGCAGGCCATTCTCAAGTTGGACACGACAACAGGCCGCAGCGAG
CAGTACAGCTTTGCCCCCCGCGGCTTTGTGGGAGAGCCGGTATTCATCCCCGATCCTGAGGACGCTGCAGAGGAAGCCGG
CTGGATTGTAACAGTGGTCTTTGATGCCGCCAGTCAGCGCTCGCAGGTGGTGATCCTGGATGCCTCAGACCTGGCTGCCG
GCCCGGTGGCGCGGATCCCCTTGCGGCATCACATTCCCTACGGCCTCCACGGCAGTTTCACCCCTCAGACATGGGTAGAG
GCCAGATAG

Upstream 100 bases:

>100_bases
ATCTTGGGGATCCCGGCCCCAGCCGCTACCTGGGCCGAGAAAGGCTGGCTACTTTACGAAAGTTTAAGCAACAATAGAAG
GCGTCGCTTGCAGACTGGCC

Downstream 100 bases:

>100_bases
GGAAGGGATCTTGGCATGGCTGGGTTAGTCCCGGGCCGCCGGGCGCGAGCTGAGGAAAAGGTGCGGCTCTGTAGCTCAAC
ATTCTGCAACCTGGGCCGGA

Product: lignostilbene-alpha,beta-dioxygenase

Products: NA

Alternate protein names: ACO; 8'-apo-beta-carotenal 15,15'-oxygenase; Diox1 [H]

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MVSVASRSYSTTDWQLGYRSLRQEYDYWATEIEGRIPERLQGTLFRNGPGKLEAGSQRYGHPFDGDGMVCAITFIDGRVH
FRNRFVRTPEFEAEEKAGRVLYRGVFGTQKPGGWWANFLDLRFKNPANTNVIYQGGKLLALWEASAPYRLNPATLATEGS
ETFAGALAPNQPFTAHPRRDPATGDLMAFGVQTGPSSTLLFYRVDAQGRLVEKRDYRLPGFAFLHDFVWTPRYWIFFQNP
VAFNPLPYMLGLQPAGTCLTLKKGEPTRIWLFPQQGEPLQLQTEPGFVFHFVNGYEVGDQVVVDAVLYEAYPALEPGADY
LQIDFAQVPAGKLWRFELDPKAGKVKRELLLDRSVEFPAIHPARMGGSHRYIYIGATHAPGPNAPLQAILKLDTTTGRSE
QYSFAPRGFVGEPVFIPDPEDAAEEAGWIVTVVFDAASQRSQVVILDASDLAAGPVARIPLRHHIPYGLHGSFTPQTWVE
AR

Sequences:

>Translated_482_residues
MVSVASRSYSTTDWQLGYRSLRQEYDYWATEIEGRIPERLQGTLFRNGPGKLEAGSQRYGHPFDGDGMVCAITFIDGRVH
FRNRFVRTPEFEAEEKAGRVLYRGVFGTQKPGGWWANFLDLRFKNPANTNVIYQGGKLLALWEASAPYRLNPATLATEGS
ETFAGALAPNQPFTAHPRRDPATGDLMAFGVQTGPSSTLLFYRVDAQGRLVEKRDYRLPGFAFLHDFVWTPRYWIFFQNP
VAFNPLPYMLGLQPAGTCLTLKKGEPTRIWLFPQQGEPLQLQTEPGFVFHFVNGYEVGDQVVVDAVLYEAYPALEPGADY
LQIDFAQVPAGKLWRFELDPKAGKVKRELLLDRSVEFPAIHPARMGGSHRYIYIGATHAPGPNAPLQAILKLDTTTGRSE
QYSFAPRGFVGEPVFIPDPEDAAEEAGWIVTVVFDAASQRSQVVILDASDLAAGPVARIPLRHHIPYGLHGSFTPQTWVE
AR
>Mature_482_residues
MVSVASRSYSTTDWQLGYRSLRQEYDYWATEIEGRIPERLQGTLFRNGPGKLEAGSQRYGHPFDGDGMVCAITFIDGRVH
FRNRFVRTPEFEAEEKAGRVLYRGVFGTQKPGGWWANFLDLRFKNPANTNVIYQGGKLLALWEASAPYRLNPATLATEGS
ETFAGALAPNQPFTAHPRRDPATGDLMAFGVQTGPSSTLLFYRVDAQGRLVEKRDYRLPGFAFLHDFVWTPRYWIFFQNP
VAFNPLPYMLGLQPAGTCLTLKKGEPTRIWLFPQQGEPLQLQTEPGFVFHFVNGYEVGDQVVVDAVLYEAYPALEPGADY
LQIDFAQVPAGKLWRFELDPKAGKVKRELLLDRSVEFPAIHPARMGGSHRYIYIGATHAPGPNAPLQAILKLDTTTGRSE
QYSFAPRGFVGEPVFIPDPEDAAEEAGWIVTVVFDAASQRSQVVILDASDLAAGPVARIPLRHHIPYGLHGSFTPQTWVE
AR

Specific function: Cleaves a number of carotenals and carotenols in the all-trans configuration at the 15-15' double bond producing retinal or retinol, respectively. Also shows activity toward lycopenals and the corresponding alcohols. Does not cleave beta- carotene or lyco

COG id: COG3670

COG function: function code Q; Lignostilbene-alpha,beta-dioxygenase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carotenoid oxygenase family [H]

Homologues:

Organism=Homo sapiens, GI74027270, Length=529, Percent_Identity=25.7088846880907, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI4506591, Length=532, Percent_Identity=22.9323308270677, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI71998242, Length=514, Percent_Identity=24.7081712062257, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24646669, Length=329, Percent_Identity=27.0516717325228, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004294 [H]

Pfam domain/function: PF03055 RPE65 [H]

EC number: =1.14.99.41 [H]

Molecular weight: Translated: 53871; Mature: 53871

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSVASRSYSTTDWQLGYRSLRQEYDYWATEIEGRIPERLQGTLFRNGPGKLEAGSQRYG
CCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCEEEECCCCCCCCCCCCCC
HPFDGDGMVCAITFIDGRVHFRNRFVRTPEFEAEEKAGRVLYRGVFGTQKPGGWWANFLD
CCCCCCCEEEEEEEECCEEEEHHCEECCCCCCCHHHCCEEEEEECCCCCCCCCCEEEEEE
LRFKNPANTNVIYQGGKLLALWEASAPYRLNPATLATEGSETFAGALAPNQPFTAHPRRD
EEECCCCCCCEEEECCEEEEEEECCCCEEECCCEEEECCCCCEEEECCCCCCCCCCCCCC
PATGDLMAFGVQTGPSSTLLFYRVDAQGRLVEKRDYRLPGFAFLHDFVWTPRYWIFFQNP
CCCCCEEEEEECCCCCCEEEEEEECCCCCEECCCCCCCCCHHHHHHHHCCCEEEEEECCC
VAFNPLPYMLGLQPAGTCLTLKKGEPTRIWLFPQQGEPLQLQTEPGFVFHFVNGYEVGDQ
CCCCCCCCEEECCCCCCEEEEECCCCCEEEEECCCCCCEEEECCCCEEEEEECCCCCCCH
VVVDAVLYEAYPALEPGADYLQIDFAQVPAGKLWRFELDPKAGKVKRELLLDRSVEFPAI
HHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCEEEEEECCCCCHHHHHHHHCCCCCCCCC
HPARMGGSHRYIYIGATHAPGPNAPLQAILKLDTTTGRSEQYSFAPRGFVGEPVFIPDPE
CHHHCCCCEEEEEEEECCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEECCCCH
DAAEEAGWIVTVVFDAASQRSQVVILDASDLAAGPVARIPLRHHIPYGLHGSFTPQTWVE
HHHHHCCEEEEEEEECCCCCCEEEEEECCCCCCCCEEECCHHHCCCCCCCCCCCCCCCCC
AR
CC
>Mature Secondary Structure
MVSVASRSYSTTDWQLGYRSLRQEYDYWATEIEGRIPERLQGTLFRNGPGKLEAGSQRYG
CCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCEEEECCCCCCCCCCCCCC
HPFDGDGMVCAITFIDGRVHFRNRFVRTPEFEAEEKAGRVLYRGVFGTQKPGGWWANFLD
CCCCCCCEEEEEEEECCEEEEHHCEECCCCCCCHHHCCEEEEEECCCCCCCCCCEEEEEE
LRFKNPANTNVIYQGGKLLALWEASAPYRLNPATLATEGSETFAGALAPNQPFTAHPRRD
EEECCCCCCCEEEECCEEEEEEECCCCEEECCCEEEECCCCCEEEECCCCCCCCCCCCCC
PATGDLMAFGVQTGPSSTLLFYRVDAQGRLVEKRDYRLPGFAFLHDFVWTPRYWIFFQNP
CCCCCEEEEEECCCCCCEEEEEEECCCCCEECCCCCCCCCHHHHHHHHCCCEEEEEECCC
VAFNPLPYMLGLQPAGTCLTLKKGEPTRIWLFPQQGEPLQLQTEPGFVFHFVNGYEVGDQ
CCCCCCCCEEECCCCCCEEEEECCCCCEEEEECCCCCCEEEECCCCEEEEEECCCCCCCH
VVVDAVLYEAYPALEPGADYLQIDFAQVPAGKLWRFELDPKAGKVKRELLLDRSVEFPAI
HHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCEEEEEECCCCCHHHHHHHHCCCCCCCCC
HPARMGGSHRYIYIGATHAPGPNAPLQAILKLDTTTGRSEQYSFAPRGFVGEPVFIPDPE
CHHHCCCCEEEEEEEECCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEECCCCH
DAAEEAGWIVTVVFDAASQRSQVVILDASDLAAGPVARIPLRHHIPYGLHGSFTPQTWVE
HHHHHCCEEEEEEEECCCCCCEEEEEECCCCCCCCEEECCHHHCCCCCCCCCCCCCCCCC
AR
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]