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The map label for this gene is thiG

Identifier: 86605751

GI number: 86605751

Start: 1072832

End: 1073605

Strand: Reverse

Name: thiG

Synonym: CYA_1056

Alternate gene names: 86605751

Gene position: 1073605-1072832 (Counterclockwise)

Preceding gene: 86605752

Following gene: 86605749

Centisome position: 36.61

GC content: 67.05

Gene sequence:

>774_bases
GTGCTCTACGGCCAAGCGCTGCCCAGCCGCCTGCTGCTGGGGACAGCCCGCTATCCTTCGCCGGCTATTTTGGAGCAGGC
GGTGGCGCGGGCCCAGCCCTGTTTGTTGACCGTTTCCCTGCGGCGCCAGGGGGCGCTGGGGCCGGAAGCTTCCCAGGGGT
TCTGGGCCTTGCTGCAGAAGCTAGGGATCCCGGTTCTGCCCAACACCGCCGGCTGCCACAGCGTGCAGGAGGTAGTCACG
ACGGCGGAGATGGCGCGGGAGGTGTTCGGCACCGACTGGATCAAGCTAGAGCTGATTGGCGATGACTACACCTTGCAACC
GGATCCAGTGCAACTGCCCGCTGCCGCCGCCGAGCTGGTTCGGCGGGGGTTTAAGGTCTTGCCCTACAGCACCGACGACC
TGGTGCTGTGCCAGCGGCTCTTGGATGCCGGCTGTGAGGTGGTCATGCCCTGGGCCGCCCCCATCGGCACTGGCAAAGGC
CCCCTCAACCCCTACAACTTGCGCCTGCTGCGGGAGCGGATCGAGGCGCCGCTAATTGTGGATGCTGGACTGGGCTTGCC
CTCCCACGCCTGCCAGGTGATGGAGTGGGGCTTCGATGGGGTGCTGTTGAATACAGCGGTGGCCCTGGCCCGGGATCCGG
TGGCGATGGCCGAGGCCTTTGCGGAAGCGGTGCGCTCAGGCCGCCGGGCCTACTTGGCCGGCGCGATGACGCCTCAAGAA
GCAGCTCAGCCCAGCACGCCAGTTCTCGGCACCCCCTTCTGGCACCAGGGTTAA

Upstream 100 bases:

>100_bases
GTGCCCGATTGCCTCCCCTCAAATCCCGCATTGTTGCCCCTCAGCGATGTCCACTCTTCTTCCTGTCTCCCCTTCAACTC
GGGCCGATGAGGATCCCTTG

Downstream 100 bases:

>100_bases
CTGCCCTATAACTCAAGCAACAGGCTTTTGCGCCTGGACGACCTGCAGGGCTCCTCCAGCGTGCAGGGTCTGCCGGACGG
CTTGCCAGCCCTGCTGCTGC

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MLYGQALPSRLLLGTARYPSPAILEQAVARAQPCLLTVSLRRQGALGPEASQGFWALLQKLGIPVLPNTAGCHSVQEVVT
TAEMAREVFGTDWIKLELIGDDYTLQPDPVQLPAAAAELVRRGFKVLPYSTDDLVLCQRLLDAGCEVVMPWAAPIGTGKG
PLNPYNLRLLRERIEAPLIVDAGLGLPSHACQVMEWGFDGVLLNTAVALARDPVAMAEAFAEAVRSGRRAYLAGAMTPQE
AAQPSTPVLGTPFWHQG

Sequences:

>Translated_257_residues
MLYGQALPSRLLLGTARYPSPAILEQAVARAQPCLLTVSLRRQGALGPEASQGFWALLQKLGIPVLPNTAGCHSVQEVVT
TAEMAREVFGTDWIKLELIGDDYTLQPDPVQLPAAAAELVRRGFKVLPYSTDDLVLCQRLLDAGCEVVMPWAAPIGTGKG
PLNPYNLRLLRERIEAPLIVDAGLGLPSHACQVMEWGFDGVLLNTAVALARDPVAMAEAFAEAVRSGRRAYLAGAMTPQE
AAQPSTPVLGTPFWHQG
>Mature_257_residues
MLYGQALPSRLLLGTARYPSPAILEQAVARAQPCLLTVSLRRQGALGPEASQGFWALLQKLGIPVLPNTAGCHSVQEVVT
TAEMAREVFGTDWIKLELIGDDYTLQPDPVQLPAAAAELVRRGFKVLPYSTDDLVLCQRLLDAGCEVVMPWAAPIGTGKG
PLNPYNLRLLRERIEAPLIVDAGLGLPSHACQVMEWGFDGVLLNTAVALARDPVAMAEAFAEAVRSGRRAYLAGAMTPQE
AAQPSTPVLGTPFWHQG

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S [H]

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family [H]

Homologues:

Organism=Escherichia coli, GI48994993, Length=249, Percent_Identity=39.3574297188755, Blast_Score=177, Evalue=8e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR008867 [H]

Pfam domain/function: PF05690 ThiG [H]

EC number: NA

Molecular weight: Translated: 27572; Mature: 27572

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYGQALPSRLLLGTARYPSPAILEQAVARAQPCLLTVSLRRQGALGPEASQGFWALLQK
CCCCHHCCHHHHHCCCCCCCHHHHHHHHHHCCCEEEEEEHHHCCCCCCCCCHHHHHHHHH
LGIPVLPNTAGCHSVQEVVTTAEMAREVFGTDWIKLELIGDDYTLQPDPVQLPAAAAELV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHHH
RRGFKVLPYSTDDLVLCQRLLDAGCEVVMPWAAPIGTGKGPLNPYNLRLLRERIEAPLIV
HCCCEECCCCCCHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCEEE
DAGLGLPSHACQVMEWGFDGVLLNTAVALARDPVAMAEAFAEAVRSGRRAYLAGAMTPQE
ECCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEECCCCHHH
AAQPSTPVLGTPFWHQG
HCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLYGQALPSRLLLGTARYPSPAILEQAVARAQPCLLTVSLRRQGALGPEASQGFWALLQK
CCCCHHCCHHHHHCCCCCCCHHHHHHHHHHCCCEEEEEEHHHCCCCCCCCCHHHHHHHHH
LGIPVLPNTAGCHSVQEVVTTAEMAREVFGTDWIKLELIGDDYTLQPDPVQLPAAAAELV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHHH
RRGFKVLPYSTDDLVLCQRLLDAGCEVVMPWAAPIGTGKGPLNPYNLRLLRERIEAPLIV
HCCCEECCCCCCHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCEEE
DAGLGLPSHACQVMEWGFDGVLLNTAVALARDPVAMAEAFAEAVRSGRRAYLAGAMTPQE
ECCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEECCCCHHH
AAQPSTPVLGTPFWHQG
HCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA