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The map label for this gene is nth

Identifier: 86605614

GI number: 86605614

Start: 921363

End: 922058

Strand: Reverse

Name: nth

Synonym: CYA_0912

Alternate gene names: 86605614

Gene position: 922058-921363 (Counterclockwise)

Preceding gene: 86605615

Following gene: 86605613

Centisome position: 31.44

GC content: 64.22

Gene sequence:

>696_bases
ATGAGCGGCAAGCGGGCTTTGCGCCAGCGGGCTTTGGAGATCCTGATCCGGCTGAAGCGCCATTACCCCAACTCTACCTG
TGCCCTCCACTACGAAACGCCGCTGCAACTGCTGGTGGCCACCATTTTGTCTGCACAGTGCACCGACGAGCGAGTCAACC
AAGTTACGCCGGAGCTGTTTCGCCGCTTTCCCGATGCCCAGGCGCTGGCGGCGGCGCCGCGGGAGGAGATCGAGGCCCTG
ATCCGTCCCACCGGCTTCTACCGCAACAAGGCTAAACACATCCAAGAGGCCTGCCGCAAGATCGTGACGGACTTCGGCGG
CCAGGTGCCCCGCACCATGCCGGAGCTGCTCACTCTGCCGGGGGTGGCCCGCAAGACGGCCAATGTGGTCCTGGCCCATG
CCTTTGGCATCAACGCTGGCGTGACTGTCGATACCCACGTCAAGCGCCTCAGCCGTCGCCTCGGCCTCACCGAGCACGAG
GATCCCGTGCGCATCGAGCGGGATCTGATGCAGTTGTTGCCGCAAGCCGACTGGGAAAATTGGTCCATTCGCCTGATCGA
GCATGGGCGGGCCATTTGCACCGCCCGCAAGCCCCTTTGCGAACAGTGTTTTCTGGCCGATCTCTGCCCCAGCGCCCCCC
TCCCGGGGCCGGCGGAGAAAACGGACAGAGAAGCCGCAACAGCCTCCCAGACTTGA

Upstream 100 bases:

>100_bases
ACGGGTGATGCAGACGGGCTTGGTGCTGCTGCTGGGGCTGGGGGTAGTGCTGATCTTCAAGGACACCCTTGCCATCGTCA
CCGGGTCGGGCTAGTTGCAG

Downstream 100 bases:

>100_bases
GCGCCTGGCATCCGGTTTTTCGGCTCCCCATCGCCCTAGAATAGGCTGCATGGACGATCAAGCGACTAGACCGACCGCCG
CGACCTCGCCTGTTCTCGTT

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 231; Mature: 230

Protein sequence:

>231_residues
MSGKRALRQRALEILIRLKRHYPNSTCALHYETPLQLLVATILSAQCTDERVNQVTPELFRRFPDAQALAAAPREEIEAL
IRPTGFYRNKAKHIQEACRKIVTDFGGQVPRTMPELLTLPGVARKTANVVLAHAFGINAGVTVDTHVKRLSRRLGLTEHE
DPVRIERDLMQLLPQADWENWSIRLIEHGRAICTARKPLCEQCFLADLCPSAPLPGPAEKTDREAATASQT

Sequences:

>Translated_231_residues
MSGKRALRQRALEILIRLKRHYPNSTCALHYETPLQLLVATILSAQCTDERVNQVTPELFRRFPDAQALAAAPREEIEAL
IRPTGFYRNKAKHIQEACRKIVTDFGGQVPRTMPELLTLPGVARKTANVVLAHAFGINAGVTVDTHVKRLSRRLGLTEHE
DPVRIERDLMQLLPQADWENWSIRLIEHGRAICTARKPLCEQCFLADLCPSAPLPGPAEKTDREAATASQT
>Mature_230_residues
SGKRALRQRALEILIRLKRHYPNSTCALHYETPLQLLVATILSAQCTDERVNQVTPELFRRFPDAQALAAAPREEIEALI
RPTGFYRNKAKHIQEACRKIVTDFGGQVPRTMPELLTLPGVARKTANVVLAHAFGINAGVTVDTHVKRLSRRLGLTEHED
PVRIERDLMQLLPQADWENWSIRLIEHGRAICTARKPLCEQCFLADLCPSAPLPGPAEKTDREAATASQT

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=179, Percent_Identity=31.2849162011173, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI190358497, Length=179, Percent_Identity=31.8435754189944, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI115298648, Length=179, Percent_Identity=31.8435754189944, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI115298650, Length=179, Percent_Identity=31.8435754189944, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI115298654, Length=179, Percent_Identity=31.8435754189944, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI115298652, Length=179, Percent_Identity=31.8435754189944, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI6912520, Length=179, Percent_Identity=31.8435754189944, Blast_Score=82, Evalue=6e-16,
Organism=Escherichia coli, GI1787920, Length=205, Percent_Identity=38.0487804878049, Blast_Score=140, Evalue=6e-35,
Organism=Escherichia coli, GI1789331, Length=186, Percent_Identity=29.0322580645161, Blast_Score=79, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17554540, Length=182, Percent_Identity=34.0659340659341, Blast_Score=111, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6324530, Length=190, Percent_Identity=30.5263157894737, Blast_Score=94, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6319304, Length=202, Percent_Identity=28.2178217821782, Blast_Score=80, Evalue=4e-16,
Organism=Drosophila melanogaster, GI45550361, Length=180, Percent_Identity=33.8888888888889, Blast_Score=112, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 25884; Mature: 25753

Theoretical pI: Translated: 8.91; Mature: 8.91

Prosite motif: PS00764 ENDONUCLEASE_III_1 ; PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGKRALRQRALEILIRLKRHYPNSTCALHYETPLQLLVATILSAQCTDERVNQVTPELF
CCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCHHHHHHHCCHHHH
RRFPDAQALAAAPREEIEALIRPTGFYRNKAKHIQEACRKIVTDFGGQVPRTMPELLTLP
HHCCCHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCC
GVARKTANVVLAHAFGINAGVTVDTHVKRLSRRLGLTEHEDPVRIERDLMQLLPQADWEN
CHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCC
WSIRLIEHGRAICTARKPLCEQCFLADLCPSAPLPGPAEKTDREAATASQT
CEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCC
>Mature Secondary Structure 
SGKRALRQRALEILIRLKRHYPNSTCALHYETPLQLLVATILSAQCTDERVNQVTPELF
CHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCHHHHHHHCCHHHH
RRFPDAQALAAAPREEIEALIRPTGFYRNKAKHIQEACRKIVTDFGGQVPRTMPELLTLP
HHCCCHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCC
GVARKTANVVLAHAFGINAGVTVDTHVKRLSRRLGLTEHEDPVRIERDLMQLLPQADWEN
CHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCC
WSIRLIEHGRAICTARKPLCEQCFLADLCPSAPLPGPAEKTDREAATASQT
CEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]