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The map label for this gene is cysW

Identifier: 86605186

GI number: 86605186

Start: 482242

End: 483081

Strand: Reverse

Name: cysW

Synonym: CYA_0468

Alternate gene names: 86605186

Gene position: 483081-482242 (Counterclockwise)

Preceding gene: 86605187

Following gene: 86605182

Centisome position: 16.47

GC content: 59.64

Gene sequence:

>840_bases
ATGAGACCTAAGGGAGTTGCCGAGGTTCAAGGGAAGCCACCCGCCAACCCTTGGCTGGCTGGCCTGCTGATTGGGATCGT
CTTGCTCTATGTGGGGCTGCTCATCGGGATCCCCCTGGTGAATGTCTTCTACCAGGCGTTGCGGGAAGGGATCCCCAGTT
TTTTTCAGCACCTGGGGGAGCGAGAGTTTCTCAGCGCAGCCCGCTTGACTTTGCTAACTGCCCTGGTTGTCGTGCCTCTC
AATACTCTTTTTGGCCTCTGCGCTGCCATAGCGCTAGCACGCCGCCGCTTTCCAGGGCGGGCCTTGCTGCTTAGCCTCAT
TGACCTGCCCTTTTCCATTTCGCCGGTAGTAGCCGGCCTGATGCTGGTGCTGATCTTTGGCCGTCAAGGTTGGCTGGGAT
CCTGGAACCTGCGGGTCATTTTTGCGTTTCCAGGCATTGTGCTGGCCACGGCTTTCATCACCTTGCCCTTTGTGGTGCGG
GAAGTATTGCCGGTGTTGGAAGAGGCCGGCACCGAGGAAGAAGAAGCCGCCAAAACTCTGGGGGCCAATTCCTGGCAGGT
CTTCTGGCGGATTACCCTGCCCAACATCCGCTGGGGCCTCTTGTATGGAGTCATCCTCACCAATGCCCGCGCCATGGGGG
AGTTTGGCGCTGTGGCGGTGGTCTCGGGCAACATCGTTGGGCGCACCCAAACCCTGCCTCTGTTTGTGGAAGATGCCTAT
CGCAGCTATGCCACAGGATCAGCCTATGCAGCCGCGGTGGTGCTTTCTTTGCTGGCGGTGGTGACCCTAGTGGCCAGGGA
GCTGCTGGAGCGGGGATCCCCTTCCCGCCGCATCTTTTAG

Upstream 100 bases:

>100_bases
TACGACTACGCCGGCGCCACGGTGATTGGGACGGTGATGCTGGTGATCTCCTTCCTTTTGCTGGCGGTGATTAACCTTCT
GCAAGGCTGGGCAAGTCGCC

Downstream 100 bases:

>100_bases
GTTCCCTCTCTCTCTAGGGAGAAAGGTCGGGGGTGAGGAGCTCCGGCAGCGGTACCCCAGTTCCCCCCAGGCCGCAGTAG
CCGCGCGGGTTTTTGGCGAG

Product: sulfate ABC transporter, permease protein CysW

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MRPKGVAEVQGKPPANPWLAGLLIGIVLLYVGLLIGIPLVNVFYQALREGIPSFFQHLGEREFLSAARLTLLTALVVVPL
NTLFGLCAAIALARRRFPGRALLLSLIDLPFSISPVVAGLMLVLIFGRQGWLGSWNLRVIFAFPGIVLATAFITLPFVVR
EVLPVLEEAGTEEEEAAKTLGANSWQVFWRITLPNIRWGLLYGVILTNARAMGEFGAVAVVSGNIVGRTQTLPLFVEDAY
RSYATGSAYAAAVVLSLLAVVTLVARELLERGSPSRRIF

Sequences:

>Translated_279_residues
MRPKGVAEVQGKPPANPWLAGLLIGIVLLYVGLLIGIPLVNVFYQALREGIPSFFQHLGEREFLSAARLTLLTALVVVPL
NTLFGLCAAIALARRRFPGRALLLSLIDLPFSISPVVAGLMLVLIFGRQGWLGSWNLRVIFAFPGIVLATAFITLPFVVR
EVLPVLEEAGTEEEEAAKTLGANSWQVFWRITLPNIRWGLLYGVILTNARAMGEFGAVAVVSGNIVGRTQTLPLFVEDAY
RSYATGSAYAAAVVLSLLAVVTLVARELLERGSPSRRIF
>Mature_279_residues
MRPKGVAEVQGKPPANPWLAGLLIGIVLLYVGLLIGIPLVNVFYQALREGIPSFFQHLGEREFLSAARLTLLTALVVVPL
NTLFGLCAAIALARRRFPGRALLLSLIDLPFSISPVVAGLMLVLIFGRQGWLGSWNLRVIFAFPGIVLATAFITLPFVVR
EVLPVLEEAGTEEEEAAKTLGANSWQVFWRITLPNIRWGLLYGVILTNARAMGEFGAVAVVSGNIVGRTQTLPLFVEDAY
RSYATGSAYAAAVVLSLLAVVTLVARELLERGSPSRRIF

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4208

COG function: function code P; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87082099, Length=261, Percent_Identity=48.6590038314176, Blast_Score=246, Evalue=1e-66,
Organism=Escherichia coli, GI1788764, Length=207, Percent_Identity=42.512077294686, Blast_Score=154, Evalue=6e-39,
Organism=Escherichia coli, GI1786980, Length=218, Percent_Identity=36.2385321100917, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1786254, Length=212, Percent_Identity=28.3018867924528, Blast_Score=64, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011866
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30264; Mature: 30264

Theoretical pI: Translated: 10.20; Mature: 10.20

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPKGVAEVQGKPPANPWLAGLLIGIVLLYVGLLIGIPLVNVFYQALREGIPSFFQHLGE
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
REFLSAARLTLLTALVVVPLNTLFGLCAAIALARRRFPGRALLLSLIDLPFSISPVVAGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHH
MLVLIFGRQGWLGSWNLRVIFAFPGIVLATAFITLPFVVREVLPVLEEAGTEEEEAAKTL
HHHHHHCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
GANSWQVFWRITLPNIRWGLLYGVILTNARAMGEFGAVAVVSGNIVGRTQTLPLFVEDAY
CCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCEECCCCCCHHHHHHHH
RSYATGSAYAAAVVLSLLAVVTLVARELLERGSPSRRIF
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MRPKGVAEVQGKPPANPWLAGLLIGIVLLYVGLLIGIPLVNVFYQALREGIPSFFQHLGE
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
REFLSAARLTLLTALVVVPLNTLFGLCAAIALARRRFPGRALLLSLIDLPFSISPVVAGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHH
MLVLIFGRQGWLGSWNLRVIFAFPGIVLATAFITLPFVVREVLPVLEEAGTEEEEAAKTL
HHHHHHCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
GANSWQVFWRITLPNIRWGLLYGVILTNARAMGEFGAVAVVSGNIVGRTQTLPLFVEDAY
CCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCEECCCCCCHHHHHHHH
RSYATGSAYAAAVVLSLLAVVTLVARELLERGSPSRRIF
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1708375 [H]