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The map label for this gene is cysT

Identifier: 86605187

GI number: 86605187

Start: 483071

End: 483883

Strand: Reverse

Name: cysT

Synonym: CYA_0469

Alternate gene names: 86605187

Gene position: 483883-483071 (Counterclockwise)

Preceding gene: 86605188

Following gene: 86605186

Centisome position: 16.5

GC content: 59.66

Gene sequence:

>813_bases
TTGCTGCAGGCGGTGTTTCCAGTGTCTTGGCCCTGGTGGCTGACCCTGGGCTATCTCTTCCTGATGTTGGTCTTGCCACT
AGCTGCCTTGGTTGCCAAGGCCAGCACCTTGGGCTGGGCAGAGTTTTGGCGGATTGCCACCGATCCCCTGGCCCTCTCTG
CCTATGAGGTTACTTTTGTAACCGCCTTGCTGGCGGCCTTAGTGAACGGTGTGATGGGCACTCTGACCGCCTGGGTGTTG
GTGCGCTACGCCTTCCCTGGGAAAAAGCTCCTCGAGGGGTTGATTGATCTGCCCTTTGCCCTGCCCACGGCTGTGGCCGG
CCTCACCCTGGCGACCGTCTACAGCAGCAACGGCTGGATAGGATCCCTGCTGGCTCCCTTGGGAATCCGCATTGCCTTTA
CCCGCTGGGGGGTGGGGGTAGCAATGCTGTTTATCTCTTTTCCCTTTGTGGTGCGGACCGTGCAGCCGGTGCTGCAGGAA
CTGGAACCGGATGTGGAGGAAGCTGCCTGGAGCTTGGGCGCCTCCCGCTGGCAGACCTTTTGCCGAGTGGTACTGCCGCA
AATTCTGCCGTCTATCCTAACGGGGGTGGCGCTGGGGTTTTCTCGCGCTGTCGGTGAGTATGGCTCCATCGTCATTATTG
CCGCCAGCATCCCCTTCGAGGATTTGATTGCGGCGGTGCTGGTCATCCAAAGACTGGAACAGTACGACTACGCCGGCGCC
ACGGTGATTGGGACGGTGATGCTGGTGATCTCCTTCCTTTTGCTGGCGGTGATTAACCTTCTGCAAGGCTGGGCAAGTCG
CCATGAGACCTAA

Upstream 100 bases:

>100_bases
GCACATAGGGCAGCAGGTTTACGTGCGCCCCAGGGTAATCAAAGCCTTCCCCATCAAGAAAACGGAAATGACAGCCATGG
CGGTGGGAGCTTAGGGAACC

Downstream 100 bases:

>100_bases
GGGAGTTGCCGAGGTTCAAGGGAAGCCACCCGCCAACCCTTGGCTGGCTGGCCTGCTGATTGGGATCGTCTTGCTCTATG
TGGGGCTGCTCATCGGGATC

Product: sulfate ABC transporter, permease protein CysT

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MLQAVFPVSWPWWLTLGYLFLMLVLPLAALVAKASTLGWAEFWRIATDPLALSAYEVTFVTALLAALVNGVMGTLTAWVL
VRYAFPGKKLLEGLIDLPFALPTAVAGLTLATVYSSNGWIGSLLAPLGIRIAFTRWGVGVAMLFISFPFVVRTVQPVLQE
LEPDVEEAAWSLGASRWQTFCRVVLPQILPSILTGVALGFSRAVGEYGSIVIIAASIPFEDLIAAVLVIQRLEQYDYAGA
TVIGTVMLVISFLLLAVINLLQGWASRHET

Sequences:

>Translated_270_residues
MLQAVFPVSWPWWLTLGYLFLMLVLPLAALVAKASTLGWAEFWRIATDPLALSAYEVTFVTALLAALVNGVMGTLTAWVL
VRYAFPGKKLLEGLIDLPFALPTAVAGLTLATVYSSNGWIGSLLAPLGIRIAFTRWGVGVAMLFISFPFVVRTVQPVLQE
LEPDVEEAAWSLGASRWQTFCRVVLPQILPSILTGVALGFSRAVGEYGSIVIIAASIPFEDLIAAVLVIQRLEQYDYAGA
TVIGTVMLVISFLLLAVINLLQGWASRHET
>Mature_270_residues
MLQAVFPVSWPWWLTLGYLFLMLVLPLAALVAKASTLGWAEFWRIATDPLALSAYEVTFVTALLAALVNGVMGTLTAWVL
VRYAFPGKKLLEGLIDLPFALPTAVAGLTLATVYSSNGWIGSLLAPLGIRIAFTRWGVGVAMLFISFPFVVRTVQPVLQE
LEPDVEEAAWSLGASRWQTFCRVVLPQILPSILTGVALGFSRAVGEYGSIVIIAASIPFEDLIAAVLVIQRLEQYDYAGA
TVIGTVMLVISFLLLAVINLLQGWASRHET

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=238, Percent_Identity=49.5798319327731, Blast_Score=244, Evalue=6e-66,
Organism=Escherichia coli, GI87082099, Length=261, Percent_Identity=36.0153256704981, Blast_Score=140, Evalue=7e-35,
Organism=Escherichia coli, GI1786980, Length=177, Percent_Identity=37.8531073446328, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1787369, Length=215, Percent_Identity=31.1627906976744, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29294; Mature: 29294

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1708375 [H]