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The map label for this gene is radA

Identifier: 86605055

GI number: 86605055

Start: 353548

End: 354966

Strand: Reverse

Name: radA

Synonym: CYA_0335

Alternate gene names: 86605055

Gene position: 354966-353548 (Counterclockwise)

Preceding gene: 86605056

Following gene: 86605054

Centisome position: 12.1

GC content: 65.68

Gene sequence:

>1419_bases
ATGGCCAAGGCCAAAACCGTCTGGGAGTGCAGCCAGTGCGGCGAGCGCTACCCCAAGTTTTTGGGCCGCTGCGACAACTG
TGGCGCCTGGAACACGCTGACGGAGGTGGTCGTCGCCCCCTCTTCTAAGTCTGGGCCACCGCAGCGGCTCGTTCCCCAGC
GCAGCCGCGGCAACGGGATGCCCCTGTCGGCCCTTCCCCTCTCCCAAGTCTCCTCGGAGAGCCACCCCCGCCTCTCCTCT
GGCTCCGGCGAGTTCGACCGGGTGTTGGGAGGTGGCATCGTGCCGGGATCCCTGGTGTTGATCGGCGGCGATCCAGGCAT
TGGCAAGAGCACCCTGCTGCTGCAGACTGCTGCGCATCTCGCCAAGCGGGGGCCCATCTTGTACGTGTCGGGGGAGGAAT
CGGCCCAGCAGATCAAGCTGCGCGCCGCTCGCATTAGCGGGACGGAGTCCTTGCGGCTGGGGATAGAAAGCGAGCAGCTC
TACCTATTGGCGGAAACCGACCTGGAGGCCATTGTGGCCGAGATCCAGGCCCTACAGCCCCAGGTGGCGATCATCGACAG
CATCCAGGCTATCTTCTGGGGGCAGCTCAGCTCGGCGCCGGGATCCGTTTCCCAGGTGCGGGAATGCGCCGGCCTCTTGA
TGCGAGTGGCCAAAAAACTGGGGGTGGCCCTGCTCATCGTTGGCCATGTTACCAAAGATGGATCCCTGGCCGGGCCCAAG
GTGCTGGAGCACCTGGTGGACACCGTCTTGTACTTTGAGGGGGATCGCTTTCAGAGCCACCGCCTGCTGCGCGCCGTCAA
AAACCGCTTTGGGGCGGCCCAGGAAATCGGCGTTTTCGAGATGACCGCCTCTGGCTTGGTGGAGGTGGACAATCCCTCGC
AGCTTTTTTTGAGCAGCCGGGATCAGGCCACGCCGGGAACGGCCACCATCGTCGCCTGTGAGGGCACCCGCCCCTTGGTG
GTGGACGTGCAGGCCCTGGTCAGCCCCACCAGCTACAGCTCCCCCCGCCGCACCGCCACCGGCATCGAGCTCAACCGCTT
TTTGCAGATCTTGGCGGTGCTGGAAAAACGGGTGGGGATCCCGCTTTCCAAATACGACGCCTACATTGCCTCCGCCGGCG
GCATCCAGGTGGCCGAGCCAGCTGCCGACTTGGGGGTGGCGGTGGCCGTGGCCGCCAGCTTCCGAGATCGCCAGGTGGAT
CCCTTTACGGTCTTGATCGGCGAGGTGGGCCTGGGGGGACAGGTGCGCCCGGTGGCGCAGTTGGAGCAGCGCCTCAAGGA
AGCCGCCAAGCTGGGGTTCCGCCACGCCATCATCCCCAACAGCCCCGTTTTGGCCGACTCTGGCCTGCGCTTGAGCCGGG
TGCAGCGGGTGTTGGAAGCTCTGCTGGTGGCTCTGCCCAGCTCCGAGCCTGGCCAGTGA

Upstream 100 bases:

>100_bases
GCCTTGCCAGAGGTGCCCATTCCCGCCGTTCCCCTGCCCCACAGCTAGCGGCAGCCGGTCGGAGTCCTTGTATCCTAAGA
AAGAGGCGGGATCCCTGTTC

Downstream 100 bases:

>100_bases
GAAGGGATCCCCAACTTGCCCAGTTGGGCGGTCGAGCTCCGCCAAGAACCAAGCTACGGGAAAATGTCATAGAATAAAGG
TTTGTGCGACAACAGCAACC

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms homolog [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MAKAKTVWECSQCGERYPKFLGRCDNCGAWNTLTEVVVAPSSKSGPPQRLVPQRSRGNGMPLSALPLSQVSSESHPRLSS
GSGEFDRVLGGGIVPGSLVLIGGDPGIGKSTLLLQTAAHLAKRGPILYVSGEESAQQIKLRAARISGTESLRLGIESEQL
YLLAETDLEAIVAEIQALQPQVAIIDSIQAIFWGQLSSAPGSVSQVRECAGLLMRVAKKLGVALLIVGHVTKDGSLAGPK
VLEHLVDTVLYFEGDRFQSHRLLRAVKNRFGAAQEIGVFEMTASGLVEVDNPSQLFLSSRDQATPGTATIVACEGTRPLV
VDVQALVSPTSYSSPRRTATGIELNRFLQILAVLEKRVGIPLSKYDAYIASAGGIQVAEPAADLGVAVAVAASFRDRQVD
PFTVLIGEVGLGGQVRPVAQLEQRLKEAAKLGFRHAIIPNSPVLADSGLRLSRVQRVLEALLVALPSSEPGQ

Sequences:

>Translated_472_residues
MAKAKTVWECSQCGERYPKFLGRCDNCGAWNTLTEVVVAPSSKSGPPQRLVPQRSRGNGMPLSALPLSQVSSESHPRLSS
GSGEFDRVLGGGIVPGSLVLIGGDPGIGKSTLLLQTAAHLAKRGPILYVSGEESAQQIKLRAARISGTESLRLGIESEQL
YLLAETDLEAIVAEIQALQPQVAIIDSIQAIFWGQLSSAPGSVSQVRECAGLLMRVAKKLGVALLIVGHVTKDGSLAGPK
VLEHLVDTVLYFEGDRFQSHRLLRAVKNRFGAAQEIGVFEMTASGLVEVDNPSQLFLSSRDQATPGTATIVACEGTRPLV
VDVQALVSPTSYSSPRRTATGIELNRFLQILAVLEKRVGIPLSKYDAYIASAGGIQVAEPAADLGVAVAVAASFRDRQVD
PFTVLIGEVGLGGQVRPVAQLEQRLKEAAKLGFRHAIIPNSPVLADSGLRLSRVQRVLEALLVALPSSEPGQ
>Mature_471_residues
AKAKTVWECSQCGERYPKFLGRCDNCGAWNTLTEVVVAPSSKSGPPQRLVPQRSRGNGMPLSALPLSQVSSESHPRLSSG
SGEFDRVLGGGIVPGSLVLIGGDPGIGKSTLLLQTAAHLAKRGPILYVSGEESAQQIKLRAARISGTESLRLGIESEQLY
LLAETDLEAIVAEIQALQPQVAIIDSIQAIFWGQLSSAPGSVSQVRECAGLLMRVAKKLGVALLIVGHVTKDGSLAGPKV
LEHLVDTVLYFEGDRFQSHRLLRAVKNRFGAAQEIGVFEMTASGLVEVDNPSQLFLSSRDQATPGTATIVACEGTRPLVV
DVQALVSPTSYSSPRRTATGIELNRFLQILAVLEKRVGIPLSKYDAYIASAGGIQVAEPAADLGVAVAVAASFRDRQVDP
FTVLIGEVGLGGQVRPVAQLEQRLKEAAKLGFRHAIIPNSPVLADSGLRLSRVQRVLEALLVALPSSEPGQ

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=466, Percent_Identity=47.8540772532189, Blast_Score=416, Evalue=1e-117,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR004504
- InterPro:   IPR008269
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC; PF05362 Lon_C [H]

EC number: NA

Molecular weight: Translated: 50036; Mature: 49905

Theoretical pI: Translated: 8.36; Mature: 8.36

Prosite motif: PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAKTVWECSQCGERYPKFLGRCDNCGAWNTLTEVVVAPSSKSGPPQRLVPQRSRGNGM
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHEECCCCCCCCCHHCCCCCCCCCCC
PLSALPLSQVSSESHPRLSSGSGEFDRVLGGGIVPGSLVLIGGDPGIGKSTLLLQTAAHL
CCCCCCHHHHCCCCCCCCCCCCCCHHHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHHHH
AKRGPILYVSGEESAQQIKLRAARISGTESLRLGIESEQLYLLAETDLEAIVAEIQALQP
HHCCCEEEEECCCCHHHHHHHHHHCCCCCCEEEECCCCEEEEEECCCHHHHHHHHHHHCC
QVAIIDSIQAIFWGQLSSAPGSVSQVRECAGLLMRVAKKLGVALLIVGHVTKDGSLAGPK
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCCHH
VLEHLVDTVLYFEGDRFQSHRLLRAVKNRFGAAQEIGVFEMTASGLVEVDNPSQLFLSSR
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHCCEEEECCCCEEEECCCHHHHCCCC
DQATPGTATIVACEGTRPLVVDVQALVSPTSYSSPRRTATGIELNRFLQILAVLEKRVGI
CCCCCCCEEEEEECCCCCEEEEHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
PLSKYDAYIASAGGIQVAEPAADLGVAVAVAASFRDRQVDPFTVLIGEVGLGGQVRPVAQ
CHHHHHHHHCCCCCCEECCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHHH
LEQRLKEAAKLGFRHAIIPNSPVLADSGLRLSRVQRVLEALLVALPSSEPGQ
HHHHHHHHHHCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
AKAKTVWECSQCGERYPKFLGRCDNCGAWNTLTEVVVAPSSKSGPPQRLVPQRSRGNGM
CCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHEECCCCCCCCCHHCCCCCCCCCCC
PLSALPLSQVSSESHPRLSSGSGEFDRVLGGGIVPGSLVLIGGDPGIGKSTLLLQTAAHL
CCCCCCHHHHCCCCCCCCCCCCCCHHHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHHHH
AKRGPILYVSGEESAQQIKLRAARISGTESLRLGIESEQLYLLAETDLEAIVAEIQALQP
HHCCCEEEEECCCCHHHHHHHHHHCCCCCCEEEECCCCEEEEEECCCHHHHHHHHHHHCC
QVAIIDSIQAIFWGQLSSAPGSVSQVRECAGLLMRVAKKLGVALLIVGHVTKDGSLAGPK
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCCHH
VLEHLVDTVLYFEGDRFQSHRLLRAVKNRFGAAQEIGVFEMTASGLVEVDNPSQLFLSSR
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHCCEEEECCCCEEEECCCHHHHCCCC
DQATPGTATIVACEGTRPLVVDVQALVSPTSYSSPRRTATGIELNRFLQILAVLEKRVGI
CCCCCCCEEEEEECCCCCEEEEHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
PLSKYDAYIASAGGIQVAEPAADLGVAVAVAASFRDRQVDPFTVLIGEVGLGGQVRPVAQ
CHHHHHHHHCCCCCCEECCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHHH
LEQRLKEAAKLGFRHAIIPNSPVLADSGLRLSRVQRVLEALLVALPSSEPGQ
HHHHHHHHHHCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]