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The map label for this gene is pep1A [H]

Identifier: 86604984

GI number: 86604984

Start: 268685

End: 270631

Strand: Reverse

Name: pep1A [H]

Synonym: CYA_0263

Alternate gene names: 86604984

Gene position: 270631-268685 (Counterclockwise)

Preceding gene: 86604985

Following gene: 86604983

Centisome position: 9.23

GC content: 59.99

Gene sequence:

>1947_bases
ATGTGGCCAACGGAAGGTCGGCAACGCGCCATCATCGAGGCGGTGACGCCGGAAATCGACGGCGGGCGCTACGCCATCAA
GCGGACGGTGGGGGAGTATGTGGTGGTGGAGGCGGACATCTTCGCCGATGGCCACGACCAACTCTCGGCGGTGCTCAGTT
TCCGCCAGGCCGGGGCAACCGACTGGGAGGAGATCTTCATGGAGCCCCTCGGCAACGACCGCTGGCGGGCCCAGTTCCAG
GTCACGCAAATCGGCCAAGCTTTCTACCGGATTCTGGCCTGGGTGGATCCCTTCAAAACCTGGCAGCAGGATCTGCACAA
GCGGGTGGCTGCCGAGCAGGATGTGGCGGTGGAGCTGTTGATCGGGGCGGAGCTCGTGGAGCGGGCCGCCCAGGCTCAAA
GCGGGGATCTGGCGGCGAAGTTGCGCAACTACGCCCAAATGCTCCGCCAAGGCGGGGATCCAGCTATCGAGATCGCCCTT
TCCCCGACGCTGGGATCCCTGATGCAGCTCTACGGCGAGCGCCGCTTCGTCACCGCCTACCCGGAGCGACGGGTCTGGGT
GGATCCGGAGAAGGCCCGCTTCAGCACCTGGTACGAGCTGTTTCCCCGCTCCTGTGGCCCTGACGAGCACACCCACGGCA
CCTTCCAGGATGTGATCGCCCGCCTGCCGCAGATTGCCGAGATGGGCTTTGATGTGCTTTACCTGCCCCCCATCCACCCC
ATCGGGCGCACCTTCCGCAAGGGCAAAAACAACGCCATGACGGCGGAGCCGGATGACGTGGGCTCCCCCTGGGCCATCGG
CGGGCCAGAAGGCGGGCACAAGGCCATCCATCCCCAACTGGGATCCCTGGAGGATTTTCATCGCTTGGTGGCGGAGGCCA
AAAAATTCAACATCGACATCGCCCTCGATATCGCCTTCCAGTGTTCGCCAGATCACCCCTATGTGAAAGAGCACCCGGAA
TGGTTTAAAAAGCGGCCCGACGGCACCATTCAATATGCGGAAAACCCGCCCAAGAAGTACCAAGATATCTACCCTCTCGA
CTTTGAGACGGAAAACTGGCAGGCCCTCTGGGAGGAGTTAAAAAGCGTTATCGAATACTGGATCGACCAAGGGGTAACCA
TCTTTCGGGTGGATAATCCCCACACCAAAGCCTTTGGCTTCTGGGAGTGGTGCATCGGCGAGATCAAGGCCAAGCACCCG
GAGACAATCTTTTTATCCGAAGCCTTTACGCGCCCCAAGCTGATGAAATCCTTGGCCAAGCTGGGCTTCACCCAATCCTA
TACCTACTTCACCTGGCGCAACGACAAATGGGGCCTGACCGAGTACTTCACCGAGCTCACCCAAACCCCGATGCGGGAGT
ACTACCGCCCCAACCTCTGGCCCAACACGCCGGATATTCTGAACGAGTTTTTGCAAAAGGGGGGACGCCCGGCTTTTATG
CTGCGCCTCATCCTGGCGGCTACGCTGGGGGCCAGCTACGGCATCTACGGCCCCGCTTTTGAACTCTGTGAAAACCGCCC
GCTCCGCCCCGGCAGCGAAGAGTATCTGGACTCAGAAAAATACCAAATCCGCCACTGGGATCTGGACGCGCCGGATAACT
TGCGGCCTCTGATCGCGCAGGTGAACCAGATCCGGCGGCAAAACCCGGCTTTGCAAAGCGACTGGAGCCTGGTGTTTCAC
CCCACCGACAACGAGGCCCTGATCTGCTACTCCAAGCGCACCTTTGATGGCAGCAACCGGATTTTGGTGGCGGTGAATCT
GGATCCGTTTCACATTCAATCTGGCTGGGTCACCCTCAACCTGGGGGCTTTGGGTTTGGAACACTGGCAAACTTTCAAGG
TGATCGACCTCTTGGACGGCCAGCAGTACTCCTGGCAGGGATCCACCAACTACATCAAGCTGGATCCCTGGACGATGCCG
GCCCATATTTTTCTCGTCGAAAGCTGA

Upstream 100 bases:

>100_bases
GATGTATCGGTAACGGCGGGCTAACGTAAAGTCGGTTGGGCATTCTGAAAGGGCAGGCTGGCAAGCCTGTGAGCAGGCAA
AGGCAAGCTGAGGGATCATG

Downstream 100 bases:

>100_bases
AGCCCGATACCAGCAGTGCAGTGATCTCATCCACGAAAGCCGCGGAGTTGTCTCGATGGGCCAAGAGATTGCCTCCCTCA
GCTTTGGCGCAGAGGACTTT

Product: alpha-amylase family protein

Products: D-Glucose 6-phosphate; D-Glucose [C]

Alternate protein names: NA

Number of amino acids: Translated: 648; Mature: 648

Protein sequence:

>648_residues
MWPTEGRQRAIIEAVTPEIDGGRYAIKRTVGEYVVVEADIFADGHDQLSAVLSFRQAGATDWEEIFMEPLGNDRWRAQFQ
VTQIGQAFYRILAWVDPFKTWQQDLHKRVAAEQDVAVELLIGAELVERAAQAQSGDLAAKLRNYAQMLRQGGDPAIEIAL
SPTLGSLMQLYGERRFVTAYPERRVWVDPEKARFSTWYELFPRSCGPDEHTHGTFQDVIARLPQIAEMGFDVLYLPPIHP
IGRTFRKGKNNAMTAEPDDVGSPWAIGGPEGGHKAIHPQLGSLEDFHRLVAEAKKFNIDIALDIAFQCSPDHPYVKEHPE
WFKKRPDGTIQYAENPPKKYQDIYPLDFETENWQALWEELKSVIEYWIDQGVTIFRVDNPHTKAFGFWEWCIGEIKAKHP
ETIFLSEAFTRPKLMKSLAKLGFTQSYTYFTWRNDKWGLTEYFTELTQTPMREYYRPNLWPNTPDILNEFLQKGGRPAFM
LRLILAATLGASYGIYGPAFELCENRPLRPGSEEYLDSEKYQIRHWDLDAPDNLRPLIAQVNQIRRQNPALQSDWSLVFH
PTDNEALICYSKRTFDGSNRILVAVNLDPFHIQSGWVTLNLGALGLEHWQTFKVIDLLDGQQYSWQGSTNYIKLDPWTMP
AHIFLVES

Sequences:

>Translated_648_residues
MWPTEGRQRAIIEAVTPEIDGGRYAIKRTVGEYVVVEADIFADGHDQLSAVLSFRQAGATDWEEIFMEPLGNDRWRAQFQ
VTQIGQAFYRILAWVDPFKTWQQDLHKRVAAEQDVAVELLIGAELVERAAQAQSGDLAAKLRNYAQMLRQGGDPAIEIAL
SPTLGSLMQLYGERRFVTAYPERRVWVDPEKARFSTWYELFPRSCGPDEHTHGTFQDVIARLPQIAEMGFDVLYLPPIHP
IGRTFRKGKNNAMTAEPDDVGSPWAIGGPEGGHKAIHPQLGSLEDFHRLVAEAKKFNIDIALDIAFQCSPDHPYVKEHPE
WFKKRPDGTIQYAENPPKKYQDIYPLDFETENWQALWEELKSVIEYWIDQGVTIFRVDNPHTKAFGFWEWCIGEIKAKHP
ETIFLSEAFTRPKLMKSLAKLGFTQSYTYFTWRNDKWGLTEYFTELTQTPMREYYRPNLWPNTPDILNEFLQKGGRPAFM
LRLILAATLGASYGIYGPAFELCENRPLRPGSEEYLDSEKYQIRHWDLDAPDNLRPLIAQVNQIRRQNPALQSDWSLVFH
PTDNEALICYSKRTFDGSNRILVAVNLDPFHIQSGWVTLNLGALGLEHWQTFKVIDLLDGQQYSWQGSTNYIKLDPWTMP
AHIFLVES
>Mature_648_residues
MWPTEGRQRAIIEAVTPEIDGGRYAIKRTVGEYVVVEADIFADGHDQLSAVLSFRQAGATDWEEIFMEPLGNDRWRAQFQ
VTQIGQAFYRILAWVDPFKTWQQDLHKRVAAEQDVAVELLIGAELVERAAQAQSGDLAAKLRNYAQMLRQGGDPAIEIAL
SPTLGSLMQLYGERRFVTAYPERRVWVDPEKARFSTWYELFPRSCGPDEHTHGTFQDVIARLPQIAEMGFDVLYLPPIHP
IGRTFRKGKNNAMTAEPDDVGSPWAIGGPEGGHKAIHPQLGSLEDFHRLVAEAKKFNIDIALDIAFQCSPDHPYVKEHPE
WFKKRPDGTIQYAENPPKKYQDIYPLDFETENWQALWEELKSVIEYWIDQGVTIFRVDNPHTKAFGFWEWCIGEIKAKHP
ETIFLSEAFTRPKLMKSLAKLGFTQSYTYFTWRNDKWGLTEYFTELTQTPMREYYRPNLWPNTPDILNEFLQKGGRPAFM
LRLILAATLGASYGIYGPAFELCENRPLRPGSEEYLDSEKYQIRHWDLDAPDNLRPLIAQVNQIRRQNPALQSDWSLVFH
PTDNEALICYSKRTFDGSNRILVAVNLDPFHIQSGWVTLNLGALGLEHWQTFKVIDLLDGQQYSWQGSTNYIKLDPWTMP
AHIFLVES

Specific function: Could convert glycogen into units that may be subsequently converted into trehalose [H]

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family. GlgE subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021828
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF11896 DUF3416 [H]

EC number: 3.2.1.93 [C]

Molecular weight: Translated: 74561; Mature: 74561

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS00019 ACTININ_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWPTEGRQRAIIEAVTPEIDGGRYAIKRTVGEYVVVEADIFADGHDQLSAVLSFRQAGAT
CCCCCCCHHHHHHHHCCCCCCCCEEEEECCCCEEEEEEEEECCCHHHHHHHHHHHHCCCC
DWEEIFMEPLGNDRWRAQFQVTQIGQAFYRILAWVDPFKTWQQDLHKRVAAEQDVAVELL
CHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCEEEEH
IGAELVERAAQAQSGDLAAKLRNYAQMLRQGGDPAIEIALSPTLGSLMQLYGERRFVTAY
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHCCCEEEEEC
PERRVWVDPEKARFSTWYELFPRSCGPDEHTHGTFQDVIARLPQIAEMGFDVLYLPPIHP
CCCCEEECCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCH
IGRTFRKGKNNAMTAEPDDVGSPWAIGGPEGGHKAIHPQLGSLEDFHRLVAEAKKFNIDI
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEE
ALDIAFQCSPDHPYVKEHPEWFKKRPDGTIQYAENPPKKYQDIYPLDFETENWQALWEEL
EEEEEEEECCCCCCCCCCCHHHHHCCCCEEEECCCCCHHHCCCCCCCCCCCCHHHHHHHH
KSVIEYWIDQGVTIFRVDNPHTKAFGFWEWCIGEIKAKHPETIFLSEAFTRPKLMKSLAK
HHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEHHHHCCHHHHHHHHH
LGFTQSYTYFTWRNDKWGLTEYFTELTQTPMREYYRPNLWPNTPDILNEFLQKGGRPAFM
CCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCHHHH
LRLILAATLGASYGIYGPAFELCENRPLRPGSEEYLDSEKYQIRHWDLDAPDNLRPLIAQ
HHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCHHHHCCCCEEEEEECCCCCCCHHHHHHH
VNQIRRQNPALQSDWSLVFHPTDNEALICYSKRTFDGSNRILVAVNLDPFHIQSGWVTLN
HHHHHHCCCCCCCCCEEEEECCCCCEEEEEEECCCCCCCEEEEEEECCCEEEECCEEEEE
LGALGLEHWQTFKVIDLLDGQQYSWQGSTNYIKLDPWTMPAHIFLVES
ECCCCHHHHHHEEEEEEECCCEEEECCCCCEEEECCCCCCEEEEEEEC
>Mature Secondary Structure
MWPTEGRQRAIIEAVTPEIDGGRYAIKRTVGEYVVVEADIFADGHDQLSAVLSFRQAGAT
CCCCCCCHHHHHHHHCCCCCCCCEEEEECCCCEEEEEEEEECCCHHHHHHHHHHHHCCCC
DWEEIFMEPLGNDRWRAQFQVTQIGQAFYRILAWVDPFKTWQQDLHKRVAAEQDVAVELL
CHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCEEEEH
IGAELVERAAQAQSGDLAAKLRNYAQMLRQGGDPAIEIALSPTLGSLMQLYGERRFVTAY
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHCCCEEEEEC
PERRVWVDPEKARFSTWYELFPRSCGPDEHTHGTFQDVIARLPQIAEMGFDVLYLPPIHP
CCCCEEECCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCH
IGRTFRKGKNNAMTAEPDDVGSPWAIGGPEGGHKAIHPQLGSLEDFHRLVAEAKKFNIDI
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEE
ALDIAFQCSPDHPYVKEHPEWFKKRPDGTIQYAENPPKKYQDIYPLDFETENWQALWEEL
EEEEEEEECCCCCCCCCCCHHHHHCCCCEEEECCCCCHHHCCCCCCCCCCCCHHHHHHHH
KSVIEYWIDQGVTIFRVDNPHTKAFGFWEWCIGEIKAKHPETIFLSEAFTRPKLMKSLAK
HHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEHHHHCCHHHHHHHHH
LGFTQSYTYFTWRNDKWGLTEYFTELTQTPMREYYRPNLWPNTPDILNEFLQKGGRPAFM
CCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCHHHH
LRLILAATLGASYGIYGPAFELCENRPLRPGSEEYLDSEKYQIRHWDLDAPDNLRPLIAQ
HHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCHHHHCCCCEEEEEECCCCCCCHHHHHHH
VNQIRRQNPALQSDWSLVFHPTDNEALICYSKRTFDGSNRILVAVNLDPFHIQSGWVTLN
HHHHHHCCCCCCCCCEEEEECCCCCEEEEEEECCCCCCCEEEEEEECCCEEEECCEEEEE
LGALGLEHWQTFKVIDLLDGQQYSWQGSTNYIKLDPWTMPAHIFLVES
ECCCCHHHHHHEEEEEEECCCEEEECCCCCEEEECCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: H2O; alpha,alpha'-Trehalose 6-phosphate [C]

Specific reaction: H2O + alpha,alpha'-Trehalose 6-phosphate --> D-Glucose 6-phosphate + D-Glucose [C]

General reaction: O-Glycosyl bond hydrolysis [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10821190; 12000953 [H]