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The map label for this gene is pmbA [H]

Identifier: 86604985

GI number: 86604985

Start: 270708

End: 272060

Strand: Reverse

Name: pmbA [H]

Synonym: CYA_0264

Alternate gene names: 86604985

Gene position: 272060-270708 (Counterclockwise)

Preceding gene: 86604986

Following gene: 86604984

Centisome position: 9.28

GC content: 63.05

Gene sequence:

>1353_bases
ATGGCCGGCTCCGTCATTTCTCCTGAAAACCTGCTGGATCTCTGCGAACAGGGTGTGCAAAAGGCTTTGGCTGCCGGAGC
TGACCAAGCAGAGGTGTTCGCCAACTCAGGCCGCGAGACGGAAGTCACCTTTGAGAAAAATGACCTCAACCTGGCCCGCA
GCAGCAGCGAGACCCTGTTCGGCATCCGGGTTTTCTGCGGCGGCAGGCTGGGCTTTGCCACCTCCAACCGCCCTGAGGAT
CTGGCGGAGACGGCTGCTGAGGCGGTGGCCATGGCCAAGGCTTCGCCGGCGGATCCCCTCAACGGCCTGCCAGATCCCCA
GCCCCTGCCCCAGGTGCCTTTTGCCCTTGACCCAGGCCTGCTGGAACTGGATGCCGCCGAGCTGACGCAACTGGGATCCC
AACTGGTGGAGTGGGTGCGCTCCCAAGACCCGCGCATCACCATCGACAGCGGCTCGTTCAGCGTAGAAGAGGATACCGTG
GCCATTGCTTCTTCCACCGGCATCCGCGCCAACTACCACAGCGTCGAGGCGGGCGGATCCCTGTTCGGCATGGCCATCGA
TGGGGACGAGGTGGGCAGCTTTGCCTACGACGGCGACAGCGTCCAGAAGCGGGCGGATCTGGTGCCGGCGCTGGAGCGGG
CTTTTGAACGCTTTGTCCAGAAATGTGTGGGGGCCTTGGGAGCTACCGAGGGAGAGAGCTTCCGCGGCCCCATCGTCCTG
CCGCCGGATGTGGTGGAAGAGTTTCTGGGGGATCTCGTCTTTGTGCTGGGGGCGGACGCGGTGCGCAAAGGCAAAAGCCC
GCTGGCGCAGAAGATGGGATCCCTGATCGCCAGCCCCCTGTTGACGTTGGTTTCCGAGGGGCTGGGCTTGCCTGGCTACC
CCATCGAGCCCTTCGACCGCGAGGGGATCCCCCGCCAAATTACCCCCCTGATCCATGAAGGGATCCTCTGCAACTTCATG
TACAACAGCTACGAAGGCCGGGCTGCCGGGATCCCGTCCAACGGCCATGCCACAGGGGGGGCGGGCAGCTTGCCGGGCGT
GGGGCCGGCCTGTTTGAGCGTGGCCCCGGGGACAACCCCTTTGTCTGAGCTGTATGCGATGGAGCGGGGCATTATCGTCA
CCCGGCTTTCCGGCAGCAGCAACCCCATCACTGGCGATTTTTCCGGGGTGGTGAAGGGGGGCTTTTTGGTTAAAGGCGGC
GAAAAACGCCCCATCCTGGAGACCACCATTGCCGGCAACCTCTACGATTGTTTGCGCAATATCTCCGCCATCTCACAAGA
GGTAACCGTTTTCAACGGCACCACCGCTTTTCCCGCCCTGCGCATCGAAGATGTATCGGTAACGGCGGGCTAA

Upstream 100 bases:

>100_bases
CCGCTTGAAACAGGCGCGCTCTTCTGGGCCCCGTTCCTGGGGCAGGCCTTGCTGCTAGGGTCGATAATAGAACTGTTCTC
TTGCGGATCTCAAGTTTTCT

Downstream 100 bases:

>100_bases
CGTAAAGTCGGTTGGGCATTCTGAAAGGGCAGGCTGGCAAGCCTGTGAGCAGGCAAAGGCAAGCTGAGGGATCATGATGT
GGCCAACGGAAGGTCGGCAA

Product: tldD/pmbA family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MAGSVISPENLLDLCEQGVQKALAAGADQAEVFANSGRETEVTFEKNDLNLARSSSETLFGIRVFCGGRLGFATSNRPED
LAETAAEAVAMAKASPADPLNGLPDPQPLPQVPFALDPGLLELDAAELTQLGSQLVEWVRSQDPRITIDSGSFSVEEDTV
AIASSTGIRANYHSVEAGGSLFGMAIDGDEVGSFAYDGDSVQKRADLVPALERAFERFVQKCVGALGATEGESFRGPIVL
PPDVVEEFLGDLVFVLGADAVRKGKSPLAQKMGSLIASPLLTLVSEGLGLPGYPIEPFDREGIPRQITPLIHEGILCNFM
YNSYEGRAAGIPSNGHATGGAGSLPGVGPACLSVAPGTTPLSELYAMERGIIVTRLSGSSNPITGDFSGVVKGGFLVKGG
EKRPILETTIAGNLYDCLRNISAISQEVTVFNGTTAFPALRIEDVSVTAG

Sequences:

>Translated_450_residues
MAGSVISPENLLDLCEQGVQKALAAGADQAEVFANSGRETEVTFEKNDLNLARSSSETLFGIRVFCGGRLGFATSNRPED
LAETAAEAVAMAKASPADPLNGLPDPQPLPQVPFALDPGLLELDAAELTQLGSQLVEWVRSQDPRITIDSGSFSVEEDTV
AIASSTGIRANYHSVEAGGSLFGMAIDGDEVGSFAYDGDSVQKRADLVPALERAFERFVQKCVGALGATEGESFRGPIVL
PPDVVEEFLGDLVFVLGADAVRKGKSPLAQKMGSLIASPLLTLVSEGLGLPGYPIEPFDREGIPRQITPLIHEGILCNFM
YNSYEGRAAGIPSNGHATGGAGSLPGVGPACLSVAPGTTPLSELYAMERGIIVTRLSGSSNPITGDFSGVVKGGFLVKGG
EKRPILETTIAGNLYDCLRNISAISQEVTVFNGTTAFPALRIEDVSVTAG
>Mature_449_residues
AGSVISPENLLDLCEQGVQKALAAGADQAEVFANSGRETEVTFEKNDLNLARSSSETLFGIRVFCGGRLGFATSNRPEDL
AETAAEAVAMAKASPADPLNGLPDPQPLPQVPFALDPGLLELDAAELTQLGSQLVEWVRSQDPRITIDSGSFSVEEDTVA
IASSTGIRANYHSVEAGGSLFGMAIDGDEVGSFAYDGDSVQKRADLVPALERAFERFVQKCVGALGATEGESFRGPIVLP
PDVVEEFLGDLVFVLGADAVRKGKSPLAQKMGSLIASPLLTLVSEGLGLPGYPIEPFDREGIPRQITPLIHEGILCNFMY
NSYEGRAAGIPSNGHATGGAGSLPGVGPACLSVAPGTTPLSELYAMERGIIVTRLSGSSNPITGDFSGVVKGGFLVKGGE
KRPILETTIAGNLYDCLRNISAISQEVTVFNGTTAFPALRIEDVSVTAG

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=463, Percent_Identity=27.4298056155508, Blast_Score=112, Evalue=7e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 46957; Mature: 46826

Theoretical pI: Translated: 4.23; Mature: 4.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGSVISPENLLDLCEQGVQKALAAGADQAEVFANSGRETEVTFEKNDLNLARSSSETLF
CCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCEEEEEECCCCCHHCCCCCCEE
GIRVFCGGRLGFATSNRPEDLAETAAEAVAMAKASPADPLNGLPDPQPLPQVPFALDPGL
EEEEEECCEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
LELDAAELTQLGSQLVEWVRSQDPRITIDSGSFSVEEDTVAIASSTGIRANYHSVEAGGS
EEECHHHHHHHHHHHHHHHHCCCCEEEEECCCCEECCCCEEEEECCCCCCCCCEECCCCC
LFGMAIDGDEVGSFAYDGDSVQKRADLVPALERAFERFVQKCVGALGATEGESFRGPIVL
EEEEEECCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEC
PPDVVEEFLGDLVFVLGADAVRKGKSPLAQKMGSLIASPLLTLVSEGLGLPGYPIEPFDR
CHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
EGIPRQITPLIHEGILCNFMYNSYEGRAAGIPSNGHATGGAGSLPGVGPACLSVAPGTTP
CCCCHHHHHHHHCCEEEEEHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCH
LSELYAMERGIIVTRLSGSSNPITGDFSGVVKGGFLVKGGEKRPILETTIAGNLYDCLRN
HHHHHHHHCCEEEEEECCCCCCCCCCCHHHHCCCEEEECCCCCCCCHHHHCCHHHHHHHH
ISAISQEVTVFNGTTAFPALRIEDVSVTAG
HHHHHHHEEEECCCCCCCEEEEEEEEEECC
>Mature Secondary Structure 
AGSVISPENLLDLCEQGVQKALAAGADQAEVFANSGRETEVTFEKNDLNLARSSSETLF
CCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCEEEEEECCCCCHHCCCCCCEE
GIRVFCGGRLGFATSNRPEDLAETAAEAVAMAKASPADPLNGLPDPQPLPQVPFALDPGL
EEEEEECCEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
LELDAAELTQLGSQLVEWVRSQDPRITIDSGSFSVEEDTVAIASSTGIRANYHSVEAGGS
EEECHHHHHHHHHHHHHHHHCCCCEEEEECCCCEECCCCEEEEECCCCCCCCCEECCCCC
LFGMAIDGDEVGSFAYDGDSVQKRADLVPALERAFERFVQKCVGALGATEGESFRGPIVL
EEEEEECCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEC
PPDVVEEFLGDLVFVLGADAVRKGKSPLAQKMGSLIASPLLTLVSEGLGLPGYPIEPFDR
CHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
EGIPRQITPLIHEGILCNFMYNSYEGRAAGIPSNGHATGGAGSLPGVGPACLSVAPGTTP
CCCCHHHHHHHHCCEEEEEHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCH
LSELYAMERGIIVTRLSGSSNPITGDFSGVVKGGFLVKGGEKRPILETTIAGNLYDCLRN
HHHHHHHHCCEEEEEECCCCCCCCCCCHHHHCCCEEEECCCCCCCCHHHHCCHHHHHHHH
ISAISQEVTVFNGTTAFPALRIEDVSVTAG
HHHHHHHEEEECCCCCCCEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]