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The map label for this gene is nnrR

Identifier: 86361256

GI number: 86361256

Start: 592646

End: 593320

Strand: Direct

Name: nnrR

Synonym: RHE_PF00527

Alternate gene names: 86361256

Gene position: 592646-593320 (Clockwise)

Preceding gene: 86361255

Following gene: 86361257

Centisome position: 92.24

GC content: 62.96

Gene sequence:

>675_bases
TTGAAAGCGCTGCCGCTCTTTGAACGGATGACGGATACGGATCTCGACGCGATGCTGCAGCATGCCGCACCGCGCCGCGT
CGCGCAGGGTGACGCGGTTTTCGAGCAGGGTGCCCTCGCAAAGTCGTTTTTCCTGCTCCTTCACGGCCGTCTCAAGGTGA
CGCAGGTGACGCCGGACGGACAGCAGATCATCGTGCGGATGGTCCACCCGGGCGATTTGTTCGGCTTCGCGATGGCGTTG
CGCAGGGAAGACTATCCCGGGACGGCTGTAGCGGCGGCCGAGAGCCTGGTCATCGGTTGGCCGACGGATATGTGGCCGCG
CTTTGTCGAGCAGAATCCCCGCCTGGCCGTAACAGCGATGCAGACGATCGGCCAGCGGCTGGAGGAAGCGCATACGCGTA
TTCGCGAGATGTCGACCGAGGAGGTGGAAAAGCGGGTGGCGCACGCCGTCCTCAGGCTGTCGAAACAGGCGGGGCGGCCG
GTCGGAAGCGGCATTCGCATCGATTTCCCGATATCGCGTCAGGATATTGCCGAAATGACCGGAACGACGCTGCACACCGT
GTCGAGAATTCTGAGCGCCTGGGAGGCGAAGGGCATGGTCGAAGGCGGCCGCCAGAAGCTGACGGTCACCGATCCTCAGA
AACTTCTCAATCTTGCCGACGGCAAGCGGGATTGA

Upstream 100 bases:

>100_bases
AGGCTGACAGAGCGGGTAGAAGGCGCAATGACCCATATGTTCGAACCGGCGATGAGCCCCACGGCAGAACAGGATGACGA
TGAAAGTTGACCGGACGGTT

Downstream 100 bases:

>100_bases
AGCTCCAGGAGAATGAGTTTGACCGAGTTCCTTGCCGCCTTCGCGCTCTTTGTCGCGCTCCATTCCATCCCGGCGGTTCC
GACCATTCGTGCGGAAATTA

Product: denitrification transcriptional regulator protein

Products: NA

Alternate protein names: DNA-binding protein VF1; Nitrogen-responsive regulatory protein [H]

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MKALPLFERMTDTDLDAMLQHAAPRRVAQGDAVFEQGALAKSFFLLLHGRLKVTQVTPDGQQIIVRMVHPGDLFGFAMAL
RREDYPGTAVAAAESLVIGWPTDMWPRFVEQNPRLAVTAMQTIGQRLEEAHTRIREMSTEEVEKRVAHAVLRLSKQAGRP
VGSGIRIDFPISRQDIAEMTGTTLHTVSRILSAWEAKGMVEGGRQKLTVTDPQKLLNLADGKRD

Sequences:

>Translated_224_residues
MKALPLFERMTDTDLDAMLQHAAPRRVAQGDAVFEQGALAKSFFLLLHGRLKVTQVTPDGQQIIVRMVHPGDLFGFAMAL
RREDYPGTAVAAAESLVIGWPTDMWPRFVEQNPRLAVTAMQTIGQRLEEAHTRIREMSTEEVEKRVAHAVLRLSKQAGRP
VGSGIRIDFPISRQDIAEMTGTTLHTVSRILSAWEAKGMVEGGRQKLTVTDPQKLLNLADGKRD
>Mature_224_residues
MKALPLFERMTDTDLDAMLQHAAPRRVAQGDAVFEQGALAKSFFLLLHGRLKVTQVTPDGQQIIVRMVHPGDLFGFAMAL
RREDYPGTAVAAAESLVIGWPTDMWPRFVEQNPRLAVTAMQTIGQRLEEAHTRIREMSTEEVEKRVAHAVLRLSKQAGRP
VGSGIRIDFPISRQDIAEMTGTTLHTVSRILSAWEAKGMVEGGRQKLTVTDPQKLLNLADGKRD

Specific function: Has affinity for the xisA upstream region. Binds to a 66 bp region containing three repeats of the consensus recognition sequence 5'-ACATT-3' [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787595, Length=210, Percent_Identity=24.7619047619048, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR022299
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 24892; Mature: 24892

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: PS50042 CNMP_BINDING_3 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKALPLFERMTDTDLDAMLQHAAPRRVAQGDAVFEQGALAKSFFLLLHGRLKVTQVTPDG
CCCCCHHHHHCCCCHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHCCEEEEEECCCH
QQIIVRMVHPGDLFGFAMALRREDYPGTAVAAAESLVIGWPTDMWPRFVEQNPRLAVTAM
HHEEEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHEEEECCHHHHHHHHCCCCCEEHHHH
QTIGQRLEEAHTRIREMSTEEVEKRVAHAVLRLSKQAGRPVGSGIRIDFPISRQDIAEMT
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHC
GTTLHTVSRILSAWEAKGMVEGGRQKLTVTDPQKLLNLADGKRD
CHHHHHHHHHHHHHHHCCHHCCCCEEEEECCHHHHHHHHCCCCC
>Mature Secondary Structure
MKALPLFERMTDTDLDAMLQHAAPRRVAQGDAVFEQGALAKSFFLLLHGRLKVTQVTPDG
CCCCCHHHHHCCCCHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHCCEEEEEECCCH
QQIIVRMVHPGDLFGFAMALRREDYPGTAVAAAESLVIGWPTDMWPRFVEQNPRLAVTAM
HHEEEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHEEEECCHHHHHHHHCCCCCEEHHHH
QTIGQRLEEAHTRIREMSTEEVEKRVAHAVLRLSKQAGRPVGSGIRIDFPISRQDIAEMT
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHC
GTTLHTVSRILSAWEAKGMVEGGRQKLTVTDPQKLLNLADGKRD
CHHHHHHHHHHHHHHHCCHHCCCCEEEEECCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11114927 [H]