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The map label for this gene is iolE [H]

Identifier: 86361176

GI number: 86361176

Start: 499432

End: 500244

Strand: Direct

Name: iolE [H]

Synonym: RHE_PF00446

Alternate gene names: 86361176

Gene position: 499432-500244 (Clockwise)

Preceding gene: 86361175

Following gene: 86361177

Centisome position: 77.73

GC content: 60.39

Gene sequence:

>813_bases
ATGGCGCTCGCCGGCTATGTCGGGTGCGGCGTTGGCCATAAATATCCGACGGATCTGAAAATCTTGCGCCCCGTCCTCGA
ACTCAGGGGATTGCGGATTTCCGAGCCTTGGGTGAGCACTTACTTCACCATCAAGGCGATGAATCGTCACACGCTTGAAA
GCGTCGATGCTCAGCTCGACTTCCTCGAAGCCATGGAAGGCGGCAGCGACGATCCGCGCAGGGCCGATCTCGTCGTCTCC
GAGTTTGGCGGAGCGGTCAATCCGCTTCCGGTCGCCCTGTTTCCCAATTGCCCCGAATTCTCCGAGGACCAGTGGAAACA
GCTGATCGAAGGCCTGCATGCAGCCGGCGAGAAGGCCAAGGCCCGCAACCGCCGGCTCTGCTATCACCCGCATCTCGGAA
CCGGGGTGATGAAGACGGAAGCGATCCACCGGCTCATGGACGAGACGGATCCTCGCCTAGTCCACATGCTTCTCGATACC
GCACACCAGGCGGCCGCCGGCGTCGATCCGCTGGCGCTGGCCAGAAAATACGCCCACCGCATCAAGCATGTTCACCTCAA
AGACATTCGCGGCGAGGTGGTTACGAACATTCACAAGGGAGAATTGTCCTTCCAGCAAGGGATCGAGGCGGGGATCTTCA
CCGTGCCGGGTGACGGCTCCATCGAGACCTTTCCGGAAATCCTCGATGCGTTGGCCGAGGCGGATTTCGCCGGGTGGATC
TGCATCGAGGCAGAGCAGGACCCGGCCAAGGCCAATCCGCTGCAATACGCGAAAATGGGCCGTGAATATCTGCGCAAGCT
GCTTGGATGGTAG

Upstream 100 bases:

>100_bases
GCCCGCTAAGGTCTGGCTCGGCGTCATCCCCACGCTCTGGTGGAATGACGATTTCATCGACATCGACATCGGCATCCCCT
ACGAGCAGGCTCTGAGTGAA

Downstream 100 bases:

>100_bases
CCCGGCGCCGAGCGCTTGTTTCAGCCGGATGGCGATGTGAAGGAGGCGGGAAAGGATCTCGCCTCATACAATGCCCGGCG
AGACATCATCCTGAACCACG

Product: myo-inositol catabolism protein

Products: NA

Alternate protein names: 2-keto-myo-inositol dehydratase; 2KMI dehydratase [H]

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MALAGYVGCGVGHKYPTDLKILRPVLELRGLRISEPWVSTYFTIKAMNRHTLESVDAQLDFLEAMEGGSDDPRRADLVVS
EFGGAVNPLPVALFPNCPEFSEDQWKQLIEGLHAAGEKAKARNRRLCYHPHLGTGVMKTEAIHRLMDETDPRLVHMLLDT
AHQAAAGVDPLALARKYAHRIKHVHLKDIRGEVVTNIHKGELSFQQGIEAGIFTVPGDGSIETFPEILDALAEADFAGWI
CIEAEQDPAKANPLQYAKMGREYLRKLLGW

Sequences:

>Translated_270_residues
MALAGYVGCGVGHKYPTDLKILRPVLELRGLRISEPWVSTYFTIKAMNRHTLESVDAQLDFLEAMEGGSDDPRRADLVVS
EFGGAVNPLPVALFPNCPEFSEDQWKQLIEGLHAAGEKAKARNRRLCYHPHLGTGVMKTEAIHRLMDETDPRLVHMLLDT
AHQAAAGVDPLALARKYAHRIKHVHLKDIRGEVVTNIHKGELSFQQGIEAGIFTVPGDGSIETFPEILDALAEADFAGWI
CIEAEQDPAKANPLQYAKMGREYLRKLLGW
>Mature_269_residues
ALAGYVGCGVGHKYPTDLKILRPVLELRGLRISEPWVSTYFTIKAMNRHTLESVDAQLDFLEAMEGGSDDPRRADLVVSE
FGGAVNPLPVALFPNCPEFSEDQWKQLIEGLHAAGEKAKARNRRLCYHPHLGTGVMKTEAIHRLMDETDPRLVHMLLDTA
HQAAAGVDPLALARKYAHRIKHVHLKDIRGEVVTNIHKGELSFQQGIEAGIFTVPGDGSIETFPEILDALAEADFAGWIC
IEAEQDPAKANPLQYAKMGREYLRKLLGW

Specific function: Catalyzes the dehydration of inosose (2-keto-myo- inositol, 2KMI or 2,4,6/3,5-pentahydroxycyclohexanone) to 3D- (3,5/4)-trihydroxycyclohexane-1,2-dione (D-2,3-diketo-4-deoxy-epi- inositol) [H]

COG id: COG1082

COG function: function code G; Sugar phosphate isomerases/epimerases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iolE/mocC family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013022
- InterPro:   IPR012307 [H]

Pfam domain/function: PF01261 AP_endonuc_2 [H]

EC number: =4.2.1.44 [H]

Molecular weight: Translated: 29967; Mature: 29836

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALAGYVGCGVGHKYPTDLKILRPVLELRGLRISEPWVSTYFTIKAMNRHTLESVDAQLD
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHEEEEECCCHHHHHHHHHHHH
FLEAMEGGSDDPRRADLVVSEFGGAVNPLPVALFPNCPEFSEDQWKQLIEGLHAAGEKAK
HHHHHCCCCCCCHHHHHHHHHHCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHCCHHHH
ARNRRLCYHPHLGTGVMKTEAIHRLMDETDPRLVHMLLDTAHQAAAGVDPLALARKYAHR
HHCCCEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
IKHVHLKDIRGEVVTNIHKGELSFQQGIEAGIFTVPGDGSIETFPEILDALAEADFAGWI
HHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCEE
CIEAEQDPAKANPLQYAKMGREYLRKLLGW
EEECCCCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ALAGYVGCGVGHKYPTDLKILRPVLELRGLRISEPWVSTYFTIKAMNRHTLESVDAQLD
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHEEEEECCCHHHHHHHHHHHH
FLEAMEGGSDDPRRADLVVSEFGGAVNPLPVALFPNCPEFSEDQWKQLIEGLHAAGEKAK
HHHHHCCCCCCCHHHHHHHHHHCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHCCHHHH
ARNRRLCYHPHLGTGVMKTEAIHRLMDETDPRLVHMLLDTAHQAAAGVDPLALARKYAHR
HHCCCEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
IKHVHLKDIRGEVVTNIHKGELSFQQGIEAGIFTVPGDGSIETFPEILDALAEADFAGWI
HHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCEE
CIEAEQDPAKANPLQYAKMGREYLRKLLGW
EEECCCCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12552129 [H]