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The map label for this gene is dadX

Identifier: 86361167

GI number: 86361167

Start: 490143

End: 491276

Strand: Reverse

Name: dadX

Synonym: RHE_PF00437

Alternate gene names: 86361167

Gene position: 491276-490143 (Counterclockwise)

Preceding gene: 86361169

Following gene: 86361166

Centisome position: 76.46

GC content: 63.84

Gene sequence:

>1134_bases
ATGGATAGCGATATTTTGGTTTCCCGCAGACGCACCGCTACGATCACGCAGGGAGCCACGGGCTATCTGACGATCGATCT
TGCCGCGCTCGGCCGCAATTATTCGAAGCTTGCATCGATGCTCGCGCCGGTCCGCGCGGGCGCCGTCGTCAAGGCAGACG
CTTATGGTCTCGGCGCCGAGCGGGTCGCCCAAACGCTCTACGATCAGGGCTGCAGGCATTTCTTCGTCGCCCAGTTCGTC
GAGGCCGTGAGGCTGCGGCCGGCTCTTGCTAAGGCTGCGCAGATATTCGTGCTGAACGGCTTGCAGCCCGGCAGCGAGCT
CGCCTGCGCCGAAAGCGGCATCGTTCCGGTTCTCAATTCGCTCGCGCAGTGGCGGCAATGGTCGGAGACGGCGCGCCGGT
TGAAGCGCAGCCTGCCGGCCGTTCTGCAGTTTGACACCGGCATGTCGCGGCTCGGTTTTCCCGAGGAAGAGAGAGCCGAA
TTGGCGGCAGCCATCCGCGACGGCGCCAATGTCGAAATCCTGTTCATCATGAGCCACCTGGCCTGCGCCGACGACCTGGA
GAGCAGCCAGAACGGCGAACAGATTGCCGAGATGGCGCGCATTGCCGATGAATTTCCCGGTTTCGACATCTCCTTCGCCA
ATTCCGGCGGCGTCTTTCTCGGCGATGCCTATCATGGTGTGCTCGCCCGCCCGGGCATCGCCCTTTACGGCGGCGCACCG
AATGCCGGCGGCAAGAACCCGATGGAACCGGTCCTCAGGCTGGATGTCGCGGTCGTGCAGACGCGCACCGTACCTTCAGG
CACCAAGATCGGCTATGGCGGCGCGTATGTGACGCGCAGCCAAACCCGCCTCGCCACCATTGCCGCCGGTTACGCCGACG
GCCTGCCGCGCAGTCTCGGCGACCGCGGCGCCGTTTATCACAAGGGCACCCGTCTGCCGATCGTGGGGCGCGTGTCGATG
GACAGCGCGACCGTCGACATCACTGCTTTGCCCGAAGGGGCGCTGAGCCTCGGGAGCCTCGTCGAAGTGCTTGGTCCCAA
CCAGACGCTCGAAGACGTGGCTGGCGATGCCGGAACGATATCTTACGAAATACTAACCGGTTTGGGCGATCGTTACGACA
GGCACTATTGCTGA

Upstream 100 bases:

>100_bases
AATTATGCGAATTATATGCAGTATGCGCAATAATAAGCGACAAGATCGTCGGCAATTTCGCTCATTATTTCATAAGCTCT
GATATCGAGATGGGGTGGAC

Downstream 100 bases:

>100_bases
GAGCCGGCCGGCATTGTTCTTGAGGAAATCATGAAAGTCATTGTTCTGGGTGCCGGCATCGTCGGCGTCACATCCGCCTA
TCAGCTGGCCAAATCAGGTC

Product: alanine racemase protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MDSDILVSRRRTATITQGATGYLTIDLAALGRNYSKLASMLAPVRAGAVVKADAYGLGAERVAQTLYDQGCRHFFVAQFV
EAVRLRPALAKAAQIFVLNGLQPGSELACAESGIVPVLNSLAQWRQWSETARRLKRSLPAVLQFDTGMSRLGFPEEERAE
LAAAIRDGANVEILFIMSHLACADDLESSQNGEQIAEMARIADEFPGFDISFANSGGVFLGDAYHGVLARPGIALYGGAP
NAGGKNPMEPVLRLDVAVVQTRTVPSGTKIGYGGAYVTRSQTRLATIAAGYADGLPRSLGDRGAVYHKGTRLPIVGRVSM
DSATVDITALPEGALSLGSLVEVLGPNQTLEDVAGDAGTISYEILTGLGDRYDRHYC

Sequences:

>Translated_377_residues
MDSDILVSRRRTATITQGATGYLTIDLAALGRNYSKLASMLAPVRAGAVVKADAYGLGAERVAQTLYDQGCRHFFVAQFV
EAVRLRPALAKAAQIFVLNGLQPGSELACAESGIVPVLNSLAQWRQWSETARRLKRSLPAVLQFDTGMSRLGFPEEERAE
LAAAIRDGANVEILFIMSHLACADDLESSQNGEQIAEMARIADEFPGFDISFANSGGVFLGDAYHGVLARPGIALYGGAP
NAGGKNPMEPVLRLDVAVVQTRTVPSGTKIGYGGAYVTRSQTRLATIAAGYADGLPRSLGDRGAVYHKGTRLPIVGRVSM
DSATVDITALPEGALSLGSLVEVLGPNQTLEDVAGDAGTISYEILTGLGDRYDRHYC
>Mature_377_residues
MDSDILVSRRRTATITQGATGYLTIDLAALGRNYSKLASMLAPVRAGAVVKADAYGLGAERVAQTLYDQGCRHFFVAQFV
EAVRLRPALAKAAQIFVLNGLQPGSELACAESGIVPVLNSLAQWRQWSETARRLKRSLPAVLQFDTGMSRLGFPEEERAE
LAAAIRDGANVEILFIMSHLACADDLESSQNGEQIAEMARIADEFPGFDISFANSGGVFLGDAYHGVLARPGIALYGGAP
NAGGKNPMEPVLRLDVAVVQTRTVPSGTKIGYGGAYVTRSQTRLATIAAGYADGLPRSLGDRGAVYHKGTRLPIVGRVSM
DSATVDITALPEGALSLGSLVEVLGPNQTLEDVAGDAGTISYEILTGLGDRYDRHYC

Specific function: Isomerizes L-alanine to D-alanine which is then oxidized to pyruvate by DadA [H]

COG id: COG0787

COG function: function code M; Alanine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alanine racemase family [H]

Homologues:

Organism=Escherichia coli, GI1787439, Length=355, Percent_Identity=32.112676056338, Blast_Score=155, Evalue=5e-39,
Organism=Escherichia coli, GI1790487, Length=366, Percent_Identity=31.6939890710383, Blast_Score=137, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000821
- InterPro:   IPR009006
- InterPro:   IPR011079
- InterPro:   IPR001608
- InterPro:   IPR020622 [H]

Pfam domain/function: PF00842 Ala_racemase_C; PF01168 Ala_racemase_N [H]

EC number: =5.1.1.1 [H]

Molecular weight: Translated: 39991; Mature: 39991

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00395 ALANINE_RACEMASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSDILVSRRRTATITQGATGYLTIDLAALGRNYSKLASMLAPVRAGAVVKADAYGLGAE
CCCCEEEECCCEEEEECCCCEEEEEEHHHHCCCHHHHHHHHHHHHCCCEEEECCCCCCHH
RVAQTLYDQGCRHFFVAQFVEAVRLRPALAKAAQIFVLNGLQPGSELACAESGIVPVLNS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCHHHCCCCCHHHHHH
LAQWRQWSETARRLKRSLPAVLQFDTGMSRLGFPEEERAELAAAIRDGANVEILFIMSHL
HHHHHHHHHHHHHHHHCCCCEEEECCCHHHCCCCHHHHHHHHHHHHCCCCEEEEEEEHHH
ACADDLESSQNGEQIAEMARIADEFPGFDISFANSGGVFLGDAYHGVLARPGIALYGGAP
HHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEECCCCCEEEECCCHHHHHCCCEEEECCCC
NAGGKNPMEPVLRLDVAVVQTRTVPSGTKIGYGGAYVTRSQTRLATIAAGYADGLPRSLG
CCCCCCCHHHHHHHHHHHEEEECCCCCCCCCCCCEEEECCCHHHHHHHHHHHCCCCHHCC
DRGAVYHKGTRLPIVGRVSMDSATVDITALPEGALSLGSLVEVLGPNQTLEDVAGDAGTI
CCCCEEECCCCCEEEEEEECCCCEEEEEECCCCHHHHHHHHHHHCCCCHHHHHCCCCCEE
SYEILTGLGDRYDRHYC
HHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MDSDILVSRRRTATITQGATGYLTIDLAALGRNYSKLASMLAPVRAGAVVKADAYGLGAE
CCCCEEEECCCEEEEECCCCEEEEEEHHHHCCCHHHHHHHHHHHHCCCEEEECCCCCCHH
RVAQTLYDQGCRHFFVAQFVEAVRLRPALAKAAQIFVLNGLQPGSELACAESGIVPVLNS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCHHHCCCCCHHHHHH
LAQWRQWSETARRLKRSLPAVLQFDTGMSRLGFPEEERAELAAAIRDGANVEILFIMSHL
HHHHHHHHHHHHHHHHCCCCEEEECCCHHHCCCCHHHHHHHHHHHHCCCCEEEEEEEHHH
ACADDLESSQNGEQIAEMARIADEFPGFDISFANSGGVFLGDAYHGVLARPGIALYGGAP
HHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEECCCCCEEEECCCHHHHHCCCEEEECCCC
NAGGKNPMEPVLRLDVAVVQTRTVPSGTKIGYGGAYVTRSQTRLATIAAGYADGLPRSLG
CCCCCCCHHHHHHHHHHHEEEECCCCCCCCCCCCEEEECCCHHHHHHHHHHHCCCCHHCC
DRGAVYHKGTRLPIVGRVSMDSATVDITALPEGALSLGSLVEVLGPNQTLEDVAGDAGTI
CCCCEEECCCCCEEEEEEECCCCEEEEEECCCCHHHHHHHHHHHCCCCHHHHHCCCCCEE
SYEILTGLGDRYDRHYC
HHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10792736 [H]