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The map label for this gene is thcR [H]

Identifier: 86361165

GI number: 86361165

Start: 487756

End: 488760

Strand: Reverse

Name: thcR [H]

Synonym: RHE_PF00435

Alternate gene names: 86361165

Gene position: 488760-487756 (Counterclockwise)

Preceding gene: 86361166

Following gene: 86361163

Centisome position: 76.07

GC content: 61.49

Gene sequence:

>1005_bases
ATGTCCGTTGGTTCCGATGCGCCCCTCCCTTATCCGGCCGGGCAAAATTCGTCATTTTGGGAAACGGCATACAGCGGAAC
AGACCCGGATGCCCTCTCGGAGATATTGTCGACATCGGCCTCTCCGATCAAGGCTGCCGCGCAAGCCAATTCCTCCATCG
CATATCGCTGCAATTTCGTCTCGGCGGGGGATCTGACCATAGCCGACTGCGCCTATGACGGCGCAATCTCGCTCAGGCGG
GAGGCGCCGAGCAGCAAAGTGATCATATTCCTGCCGGTTGAGGGAAATGCAGTCTTCGATGCCGGACAGGAACCGATCCA
TTCCGTTCCGGGTCGCGGCGCCATCCTGGGAGCGGGTCGCGCCACCGGCGCCCGTCTGCTCGGCTCTCGCCGCCATCTCG
GCTTATTCGTCGATCAGGCGAGGATCAACGGGCAACTCACGCATATGTTCGAGCGAACGATCACCGGTGATGCCGGTTTT
CTTCCCCATTTCGACCTGACGGCTGGCCCGGGGCTGTTGTTGCGGCAACTCGTTGCGAACCTGTATCGTGGACTTGGCGG
GGGCGGGCTGCTGCAGGAATCGCCGCTGGCCGTCAGCGCGCTCTGCGACGCGGCGATCTTCCTGCTTCTCGAGACCTTTC
CGCATCGCTATTCGAACGAGCTGGCGCTTCCTGCGCCTGCGCCGGCTCCGCGCCATGTGAAATGGGCCATCGATTTCATG
CATGCGCACATCACGGAACCGATCTCGCTGAATGAGATCGCGATGGCAGCCAAGGTGAGCATTCGGACGTTGCAGCAGGG
CTTCCGGCAGTTCAGGAACACCACTCCGATCGCCTATCTCCATGAAATCCGCATGGTCGCCGCCCGTCGAGACCTCGTCG
AACCCGGTGCGAAACCAGCTGTCGCCGATGTCGCGCTCAGATGGGGATTTACCCATCTCGGGCGTTTTGCCGCCGAATAC
AAGAAGCGTTTCGGCGAGCTGCCGTCGCAGACGCTGAAGCGCTGA

Upstream 100 bases:

>100_bases
TCAGCCGCTACGCGTGAAATCCCGGCGAAGTAGCGCAAAGAAAGAAGAGTATTATCCCAGAGAATAACTATACTCGTGGG
AAATTATCGTGAGGTGGTGA

Downstream 100 bases:

>100_bases
ACTCCGCCTCGGCGTCCCAGACGGTTCCATCGGCGACGGACCGAGCGTCCGATCGCCGACGGCAAATGCTCTAGGCCGCG
CGTTTCAGGGCCTCCCCAAG

Product: AraC family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MSVGSDAPLPYPAGQNSSFWETAYSGTDPDALSEILSTSASPIKAAAQANSSIAYRCNFVSAGDLTIADCAYDGAISLRR
EAPSSKVIIFLPVEGNAVFDAGQEPIHSVPGRGAILGAGRATGARLLGSRRHLGLFVDQARINGQLTHMFERTITGDAGF
LPHFDLTAGPGLLLRQLVANLYRGLGGGGLLQESPLAVSALCDAAIFLLLETFPHRYSNELALPAPAPAPRHVKWAIDFM
HAHITEPISLNEIAMAAKVSIRTLQQGFRQFRNTTPIAYLHEIRMVAARRDLVEPGAKPAVADVALRWGFTHLGRFAAEY
KKRFGELPSQTLKR

Sequences:

>Translated_334_residues
MSVGSDAPLPYPAGQNSSFWETAYSGTDPDALSEILSTSASPIKAAAQANSSIAYRCNFVSAGDLTIADCAYDGAISLRR
EAPSSKVIIFLPVEGNAVFDAGQEPIHSVPGRGAILGAGRATGARLLGSRRHLGLFVDQARINGQLTHMFERTITGDAGF
LPHFDLTAGPGLLLRQLVANLYRGLGGGGLLQESPLAVSALCDAAIFLLLETFPHRYSNELALPAPAPAPRHVKWAIDFM
HAHITEPISLNEIAMAAKVSIRTLQQGFRQFRNTTPIAYLHEIRMVAARRDLVEPGAKPAVADVALRWGFTHLGRFAAEY
KKRFGELPSQTLKR
>Mature_333_residues
SVGSDAPLPYPAGQNSSFWETAYSGTDPDALSEILSTSASPIKAAAQANSSIAYRCNFVSAGDLTIADCAYDGAISLRRE
APSSKVIIFLPVEGNAVFDAGQEPIHSVPGRGAILGAGRATGARLLGSRRHLGLFVDQARINGQLTHMFERTITGDAGFL
PHFDLTAGPGLLLRQLVANLYRGLGGGGLLQESPLAVSALCDAAIFLLLETFPHRYSNELALPAPAPAPRHVKWAIDFMH
AHITEPISLNEIAMAAKVSIRTLQQGFRQFRNTTPIAYLHEIRMVAARRDLVEPGAKPAVADVALRWGFTHLGRFAAEYK
KRFGELPSQTLKR

Specific function: Probably involved in the positive regulation of the thc operon for the degradation of the thiocarbamate herbicide EPTC [H]

COG id: COG2207

COG function: function code K; AraC-type DNA-binding domain-containing proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788778, Length=100, Percent_Identity=38, Blast_Score=78, Evalue=9e-16,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060 [H]

Pfam domain/function: PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 35908; Mature: 35777

Theoretical pI: Translated: 8.79; Mature: 8.79

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVGSDAPLPYPAGQNSSFWETAYSGTDPDALSEILSTSASPIKAAAQANSSIAYRCNFV
CCCCCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHCCCCEEEEEEEE
SAGDLTIADCAYDGAISLRREAPSSKVIIFLPVEGNAVFDAGQEPIHSVPGRGAILGAGR
ECCCEEEECCCCCCCHHHHCCCCCCEEEEEEECCCCEEECCCCCHHHHCCCCCEEEECCC
ATGARLLGSRRHLGLFVDQARINGQLTHMFERTITGDAGFLPHFDLTAGPGLLLRQLVAN
CHHHHHHCCCCCCEEEEEHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHH
LYRGLGGGGLLQESPLAVSALCDAAIFLLLETFPHRYSNELALPAPAPAPRHVKWAIDFM
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCCCCCCCCHHHHHHHH
HAHITEPISLNEIAMAAKVSIRTLQQGFRQFRNTTPIAYLHEIRMVAARRDLVEPGAKPA
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCH
VADVALRWGFTHLGRFAAEYKKRFGELPSQTLKR
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCC
>Mature Secondary Structure 
SVGSDAPLPYPAGQNSSFWETAYSGTDPDALSEILSTSASPIKAAAQANSSIAYRCNFV
CCCCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHCCCCEEEEEEEE
SAGDLTIADCAYDGAISLRREAPSSKVIIFLPVEGNAVFDAGQEPIHSVPGRGAILGAGR
ECCCEEEECCCCCCCHHHHCCCCCCEEEEEEECCCCEEECCCCCHHHHCCCCCEEEECCC
ATGARLLGSRRHLGLFVDQARINGQLTHMFERTITGDAGFLPHFDLTAGPGLLLRQLVAN
CHHHHHHCCCCCCEEEEEHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHH
LYRGLGGGGLLQESPLAVSALCDAAIFLLLETFPHRYSNELALPAPAPAPRHVKWAIDFM
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCCCCCCCCHHHHHHHH
HAHITEPISLNEIAMAAKVSIRTLQQGFRQFRNTTPIAYLHEIRMVAARRDLVEPGAKPA
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCH
VADVALRWGFTHLGRFAAEYKKRFGELPSQTLKR
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]