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The map label for this gene is puuR [H]

Identifier: 86361163

GI number: 86361163

Start: 485660

End: 486271

Strand: Reverse

Name: puuR [H]

Synonym: RHE_PF00433

Alternate gene names: 86361163

Gene position: 486271-485660 (Counterclockwise)

Preceding gene: 86361165

Following gene: 86361157

Centisome position: 75.68

GC content: 58.01

Gene sequence:

>612_bases
TTGAAATTGAACAATGAAGAAGATAGGTTTTGCGATATTCTGGACAACGCAAACGGATCCATCATGTCAGTCGACATCGG
CAGCCGCCTTCGCCACCTGCGCATCGCTCACAAGCTTTCCCAGCGTGAGCTCGCCAAACGCACCGGTGTACCGAATTCGA
CGATCTCACTGATCGAATCGAATGCCTCCAATCCTTCGGTCGGAGCTCTGAAGCGAATCCTCGATGGCATTCCGATCGGG
CTGGCGGAATTCTTCGCCTTCGAACCGGAGCGTCCGAAAAAGGCCTTTTACGCCGCCCAAGAACTGGTGGAGATCGGCAA
GGGTGCCATTTCCTACAAGCAGGTCGGCGAAACCCTGTTCGGGCGCAGCCTGCAGATCCTGAAGGAATGCTACCAGCCGG
GTGCCGACACCGGAAAAATTCCCCTTGTTCACGAAGGGGAGGAGGGCGGAATCGTGCTCTCGGGAAGACTCGAGGTCACG
GTCGACGACGAGCGGCGGATCCTTGGGCCGGGAGACGCTTATTATTTCGAAAGCCGGCGCCCGCATCGGTTCCGCTGCGT
CGGACCGGTGCCCTGCGAGGTTATCAGCGCCTGCACGCCACCGACCTTTTAA

Upstream 100 bases:

>100_bases
CGTCTTGCTGATCTGGTCCATCCTGGCCTCCTTTGAAATCGCCTGGAGAATGGCAATTTCAATCTTTCAAACAAGTCCTT
TTCTTCGGTGATGCAAGTTA

Downstream 100 bases:

>100_bases
CGCAATTTCAGAATGCGTGCAGTGTTTTCGGAAGGATGATCCCGAGGCGGATCGGTAAGGATCCGCCTTCGGCGGTTGAT
CTTGACTTAGTCGTTCGCCG

Product: MerR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 203; Mature: 203

Protein sequence:

>203_residues
MKLNNEEDRFCDILDNANGSIMSVDIGSRLRHLRIAHKLSQRELAKRTGVPNSTISLIESNASNPSVGALKRILDGIPIG
LAEFFAFEPERPKKAFYAAQELVEIGKGAISYKQVGETLFGRSLQILKECYQPGADTGKIPLVHEGEEGGIVLSGRLEVT
VDDERRILGPGDAYYFESRRPHRFRCVGPVPCEVISACTPPTF

Sequences:

>Translated_203_residues
MKLNNEEDRFCDILDNANGSIMSVDIGSRLRHLRIAHKLSQRELAKRTGVPNSTISLIESNASNPSVGALKRILDGIPIG
LAEFFAFEPERPKKAFYAAQELVEIGKGAISYKQVGETLFGRSLQILKECYQPGADTGKIPLVHEGEEGGIVLSGRLEVT
VDDERRILGPGDAYYFESRRPHRFRCVGPVPCEVISACTPPTF
>Mature_203_residues
MKLNNEEDRFCDILDNANGSIMSVDIGSRLRHLRIAHKLSQRELAKRTGVPNSTISLIESNASNPSVGALKRILDGIPIG
LAEFFAFEPERPKKAFYAAQELVEIGKGAISYKQVGETLFGRSLQILKECYQPGADTGKIPLVHEGEEGGIVLSGRLEVT
VDDERRILGPGDAYYFESRRPHRFRCVGPVPCEVISACTPPTF

Specific function: Represses puuD [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH cro/C1-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787557, Length=179, Percent_Identity=35.7541899441341, Blast_Score=108, Evalue=2e-25,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013096
- InterPro:   IPR011051
- InterPro:   IPR001387
- InterPro:   IPR010982
- InterPro:   IPR014710 [H]

Pfam domain/function: PF07883 Cupin_2; PF01381 HTH_3 [H]

EC number: NA

Molecular weight: Translated: 22371; Mature: 22371

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS50943 HTH_CROC1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLNNEEDRFCDILDNANGSIMSVDIGSRLRHLRIAHKLSQRELAKRTGVPNSTISLIES
CCCCCCHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHEEC
NASNPSVGALKRILDGIPIGLAEFFAFEPERPKKAFYAAQELVEIGKGAISYKQVGETLF
CCCCCCHHHHHHHHCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
GRSLQILKECYQPGADTGKIPLVHEGEEGGIVLSGRLEVTVDDERRILGPGDAYYFESRR
HHHHHHHHHHHCCCCCCCCCCEEEECCCCCEEEECEEEEEECCCCEEECCCCCEEECCCC
PHRFRCVGPVPCEVISACTPPTF
CCEEEEECCCCHHHHHCCCCCCC
>Mature Secondary Structure
MKLNNEEDRFCDILDNANGSIMSVDIGSRLRHLRIAHKLSQRELAKRTGVPNSTISLIES
CCCCCCHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHEEC
NASNPSVGALKRILDGIPIGLAEFFAFEPERPKKAFYAAQELVEIGKGAISYKQVGETLF
CCCCCCHHHHHHHHCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
GRSLQILKECYQPGADTGKIPLVHEGEEGGIVLSGRLEVTVDDERRILGPGDAYYFESRR
HHHHHHHHHHHCCCCCCCCCCEEEECCCCCEEEECEEEEEECCCCEEECCCCCEEECCCC
PHRFRCVGPVPCEVISACTPPTF
CCEEEEECCCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553 [H]