Click here to switch to the map view.

The map label for this gene is gbsB [H]

Identifier: 86361095

GI number: 86361095

Start: 413191

End: 414420

Strand: Reverse

Name: gbsB [H]

Synonym: RHE_PF00365

Alternate gene names: 86361095

Gene position: 414420-413191 (Counterclockwise)

Preceding gene: 86361096

Following gene: 86361094

Centisome position: 64.5

GC content: 65.12

Gene sequence:

>1230_bases
ATGAGCCTGTTTGCCGCCTTGAGGCTGCCGCGGGAGATCCTGTTCGGTATCGGCCAGCGCCATGCGCTCGCGAGCGTCGC
CAGCAGGACCGGCCGGCGCGCGCTCGTCTGCACGGACGCGCGTTTTGCCTCAACGCCCGCCTTCGCTGAAATGATCTCCG
CCATCGAAGCCGCCGGCATCTCCGTACTGGTCCATGATCAGACGCTGCCGGACGTGCCGCGCGACACGGTCGCCGCTTGC
GTTGAGGCCGCGCGTGGCTTTAAGCCCGATATGGTGATCGGCATCGGCGGTGGCAGCTGCCTCGACATGGCGAAATGCGC
GTCGCTGTTGCTCTCTCATGGCGGCGCGCTTTCCGATTACTATGGTGAGTTCAAGGTGCCGGCACCGGTCCTGCCGATCA
TTGCTGTGCCGACCACGGCAGGCACAGGCTCCGAGGTGACGCCGGTGGCGGTTGTATCCGATCCAGACCGCACCCTGAAG
GTTGGCATTTCGAGCCCCTATCTCATCGCCGCCGCGGCGATATGTGATCCGGATCTCACCGTATCCTGCCCGCCTGGGCT
CACGGCGGTTGCCGGTGCGGATGCCCTGACGCATGCCATCGAAGCCTTCACGGCGATGCGTCGCCCCGGCGACCCGGAAC
TTGCGCAGCAGCATGTGTTTGTCGGCAAGAGCGACCTGTCCGACCAGTTCGCACTATATGCTATCCGGCTCCTCAGCCGG
AGCCTTGAAAAGGCCTTCACCGACGGTTCGGACATCGAAGCGCGCTGCGATGTGATGATGGGTGCGCTCGCGGCGGGCTG
TGCCTTCGGAACCGCAGGCACCGCTGCCGCCCATGCGGTGCAATACCCTGTCGGTGCTCTGACCCATACGCCGCACGGGC
TCGGGGTGGCTACGATGCTGCCCTATGTAATGACATTCAACCGCCGCGTCGCTGCAGCGGAGATTGCCGCTGTCGGTAGG
GCGCTCGGCCTCGACCCCAATGGCGTTGAGAACGACACGGCGGTTGCCGATTCCGCAATAACCGAGGTGAGCCGCCTCTT
TGCCACGATCGGGATTGCGCCGACGCTCGCCAGACTCGGCCTACCTGAAGACCGGATCGACTGGGCGGCCGAGCAGGCAC
TCGGCATCGGCCGGCTGATCAAAAATAATCCACGGCAGCTCGATTCAGCCGCCATGCGGGGCCTGTTGCGGGCAGCCTAT
GACGGCGACCTTGCCGCTTCCGCCCTCTGA

Upstream 100 bases:

>100_bases
CGGCTGCCCGCCGCAACGGTTTTGAGATCGACCAATGCCTCTGGGAAGACCTCAACGCGCTTTCCTCCTCCCGCATTCTG
GCCTTCGAGGGACAAGGATC

Downstream 100 bases:

>100_bases
ACCGGGAAATCATCATGAACATCAACAAGACTTCTCCAAATCTAGACTACGATCCCTTCGCGCTCAACTCGTTTTCCCGC
GGCCTCTATATTGGCGGGGT

Product: putative alcohol dehydrogenase protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MSLFAALRLPREILFGIGQRHALASVASRTGRRALVCTDARFASTPAFAEMISAIEAAGISVLVHDQTLPDVPRDTVAAC
VEAARGFKPDMVIGIGGGSCLDMAKCASLLLSHGGALSDYYGEFKVPAPVLPIIAVPTTAGTGSEVTPVAVVSDPDRTLK
VGISSPYLIAAAAICDPDLTVSCPPGLTAVAGADALTHAIEAFTAMRRPGDPELAQQHVFVGKSDLSDQFALYAIRLLSR
SLEKAFTDGSDIEARCDVMMGALAAGCAFGTAGTAAAHAVQYPVGALTHTPHGLGVATMLPYVMTFNRRVAAAEIAAVGR
ALGLDPNGVENDTAVADSAITEVSRLFATIGIAPTLARLGLPEDRIDWAAEQALGIGRLIKNNPRQLDSAAMRGLLRAAY
DGDLAASAL

Sequences:

>Translated_409_residues
MSLFAALRLPREILFGIGQRHALASVASRTGRRALVCTDARFASTPAFAEMISAIEAAGISVLVHDQTLPDVPRDTVAAC
VEAARGFKPDMVIGIGGGSCLDMAKCASLLLSHGGALSDYYGEFKVPAPVLPIIAVPTTAGTGSEVTPVAVVSDPDRTLK
VGISSPYLIAAAAICDPDLTVSCPPGLTAVAGADALTHAIEAFTAMRRPGDPELAQQHVFVGKSDLSDQFALYAIRLLSR
SLEKAFTDGSDIEARCDVMMGALAAGCAFGTAGTAAAHAVQYPVGALTHTPHGLGVATMLPYVMTFNRRVAAAEIAAVGR
ALGLDPNGVENDTAVADSAITEVSRLFATIGIAPTLARLGLPEDRIDWAAEQALGIGRLIKNNPRQLDSAAMRGLLRAAY
DGDLAASAL
>Mature_408_residues
SLFAALRLPREILFGIGQRHALASVASRTGRRALVCTDARFASTPAFAEMISAIEAAGISVLVHDQTLPDVPRDTVAACV
EAARGFKPDMVIGIGGGSCLDMAKCASLLLSHGGALSDYYGEFKVPAPVLPIIAVPTTAGTGSEVTPVAVVSDPDRTLKV
GISSPYLIAAAAICDPDLTVSCPPGLTAVAGADALTHAIEAFTAMRRPGDPELAQQHVFVGKSDLSDQFALYAIRLLSRS
LEKAFTDGSDIEARCDVMMGALAAGCAFGTAGTAAAHAVQYPVGALTHTPHGLGVATMLPYVMTFNRRVAAAEIAAVGRA
LGLDPNGVENDTAVADSAITEVSRLFATIGIAPTLARLGLPEDRIDWAAEQALGIGRLIKNNPRQLDSAAMRGLLRAAYD
GDLAASAL

Specific function: Essential for the utilization of choline as a precursor [H]

COG id: COG1454

COG function: function code C; Alcohol dehydrogenase, class IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI133922590, Length=318, Percent_Identity=29.559748427673, Blast_Score=113, Evalue=3e-25,
Organism=Escherichia coli, GI48994951, Length=342, Percent_Identity=32.1637426900585, Blast_Score=169, Evalue=4e-43,
Organism=Escherichia coli, GI87082107, Length=323, Percent_Identity=36.5325077399381, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI1789163, Length=377, Percent_Identity=29.1777188328912, Blast_Score=144, Evalue=8e-36,
Organism=Escherichia coli, GI1787493, Length=431, Percent_Identity=30.3944315545244, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI1789386, Length=364, Percent_Identity=23.0769230769231, Blast_Score=72, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17537053, Length=358, Percent_Identity=29.0502793296089, Blast_Score=135, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6321181, Length=407, Percent_Identity=29.7297297297297, Blast_Score=170, Evalue=3e-43,
Organism=Drosophila melanogaster, GI24657991, Length=404, Percent_Identity=30.1980198019802, Blast_Score=130, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: =1.1.1.1 [H]

Molecular weight: Translated: 42228; Mature: 42097

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLFAALRLPREILFGIGQRHALASVASRTGRRALVCTDARFASTPAFAEMISAIEAAGI
CCHHHHHHCCHHHHHHCCCHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHCCC
SVLVHDQTLPDVPRDTVAACVEAARGFKPDMVIGIGGGSCLDMAKCASLLLSHGGALSDY
EEEEECCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCHHHH
YGEFKVPAPVLPIIAVPTTAGTGSEVTPVAVVSDPDRTLKVGISSPYLIAAAAICDPDLT
CCCCCCCCCCCCEEEECCCCCCCCCCCEEEEECCCCCEEEEECCCCHHHHHHHHCCCCCE
VSCPPGLTAVAGADALTHAIEAFTAMRRPGDPELAQQHVFVGKSDLSDQFALYAIRLLSR
EECCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHEECCCCCCCHHHHHHHHHHHHH
SLEKAFTDGSDIEARCDVMMGALAAGCAFGTAGTAAAHAVQYPVGALTHTPHGLGVATML
HHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHCCCCCCCCHHHHH
PYVMTFNRRVAAAEIAAVGRALGLDPNGVENDTAVADSAITEVSRLFATIGIAPTLARLG
HHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LPEDRIDWAAEQALGIGRLIKNNPRQLDSAAMRGLLRAAYDGDLAASAL
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHCCC
>Mature Secondary Structure 
SLFAALRLPREILFGIGQRHALASVASRTGRRALVCTDARFASTPAFAEMISAIEAAGI
CHHHHHHCCHHHHHHCCCHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHCCC
SVLVHDQTLPDVPRDTVAACVEAARGFKPDMVIGIGGGSCLDMAKCASLLLSHGGALSDY
EEEEECCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCHHHH
YGEFKVPAPVLPIIAVPTTAGTGSEVTPVAVVSDPDRTLKVGISSPYLIAAAAICDPDLT
CCCCCCCCCCCCEEEECCCCCCCCCCCEEEEECCCCCEEEEECCCCHHHHHHHHCCCCCE
VSCPPGLTAVAGADALTHAIEAFTAMRRPGDPELAQQHVFVGKSDLSDQFALYAIRLLSR
EECCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHEECCCCCCCHHHHHHHHHHHHH
SLEKAFTDGSDIEARCDVMMGALAAGCAFGTAGTAAAHAVQYPVGALTHTPHGLGVATML
HHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHCCCCCCCCHHHHH
PYVMTFNRRVAAAEIAAVGRALGLDPNGVENDTAVADSAITEVSRLFATIGIAPTLARLG
HHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LPEDRIDWAAEQALGIGRLIKNNPRQLDSAAMRGLLRAAYDGDLAASAL
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8752328; 9384377 [H]