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The map label for this gene is bioA [C]

Identifier: 86361079

GI number: 86361079

Start: 390889

End: 392265

Strand: Direct

Name: bioA [C]

Synonym: RHE_PF00349

Alternate gene names: 86361079

Gene position: 390889-392265 (Clockwise)

Preceding gene: 86361078

Following gene: 86361080

Centisome position: 60.84

GC content: 64.63

Gene sequence:

>1377_bases
ATGTACGCCAACTCGCTCATCGAACTCGACCGCGCCCATCTCATCCATCCGGTGGCCTCCTACCGCAGTCACGAGAAGCA
TGGCGTGCGCGTGCTGGCCTCGGCCAAGGGCGCGACGGTCACCGATGCCTCCGGCAAACAGCTGATCGACGGCTTCGCCG
GCCTCTGGTGCGTCAATGCCGGTTACGGCCATGAGAGCATCGTCGAGGCAGCGGCCCGGCAGATGCGGGAGCTTCCCTAT
GCAACGGCCTATTTCGGCCTTGGCTCCGAGCCGGCGATCCGGCTTGCCTCAGAACTCGCCGATCGCGCGCCTGGTGATCT
CAACCATGTCTACTTCACGCTCGGCGGCTCCGACGCAGTCGACAGCACAATCCGGTTCATTCGCTACTATTGGAATGCCC
GAGGCCGTCCCGAGCGCGATCAATTCATCTCGGTCGAACAGGGTTATCACGGTTCCTCGACCGCGGGTGCGGGTTTGACC
GCCTTGCCTGCCTTCCATGCCGGCTTCGGCCTTCCCTTCGATTGGCAGCATAAAATTCCGTCTCATTACGCCTACCGCAA
CCCGGTCGGCGAGGATCCGCAGGCGATCATTGAAGCCTCGCTCGCGGCGCTGAAAAGCAAGGTCGAGGCGATCGGGCCGG
AGCTGGTCGCCGCCTTCTACGTCGAGCCGATCCAGGGTTCGGGCGGCGTGCTCGTGCCGCCCAAAGGCTGGGTTAAGGCC
ATGCGGGAATTCTGCCGCGCGCACGACATCCTTTTCGTGGCCGATGAGGTGATCACCGGCTTTGGCCGCACCGGGCCGCT
CTTTGCCTGCAGCGAGGAAGAGGTCGTGCCGGACTTCATGACGACCGCCAAGGGCCTCACCTCCGGCTATGTCCCGATGG
GCGCCGTCTTCATGGCCGATCACGTCTATGAAACGATTGCCGAGGGGGCCGGCACCACCGCTATCGGTCACGGGTACACC
TACTCGGCCCATCCCGTCAGCGCCGCCGTCGGCCTGGAAGTCCTGAAGCTTTATGAAAACGGCCTTTTTGACAATGGCGT
CAAGGCTGGTGCGCGGCTGATGCAGGGCCTCCAATCGCTGAGAGATCACCCGCTCGTGGGCGATGTCCGCGGCCGCGGCA
TGCTCGCCGCCGTCGAGCTCGTCGTCGACAAAAGCAACAAGACGCCGCTGCCGGCGTCCGCCGAACCCGCCCGTCGGATC
TTCGATCGCGCCTGGGAGAACGGCCTCGTCATCCGCGCCTTCGGCAACGGCGTCCTCGGCTATGCGCCGCCCCTATGCTG
CACCGAAACGGAAATCGATGCCATCGTCGAGCGCACGCGCATCACGCTGGACGAGACGCTTGAGGACCCGGATGTGCGCC
GGGCGTTGCGGGTCTGA

Upstream 100 bases:

>100_bases
GCGGTATCGCAATGAAAAAACCATCGGTCGGCCACGCTGCCGCCCCTGCTGCCACCACGTTCGCGCTTGCCAGCCAGGCG
CTCGGCTGATCGGAGACCTC

Downstream 100 bases:

>100_bases
GAATAGCGCCAAACGGTGAGATTCGGTATTTTGTGCCGCTCGATGGCGTCTTTTCGGAGAATCCGGCGCAGTGCAGGTGA
CAAACTGAAATCGACGAAAG

Product: hypothetical protein

Products: S-Adenosyl-4-methylthio-2-oxobutanoate; 7,8-Diaminononanoate [C]

Alternate protein names: NA

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MYANSLIELDRAHLIHPVASYRSHEKHGVRVLASAKGATVTDASGKQLIDGFAGLWCVNAGYGHESIVEAAARQMRELPY
ATAYFGLGSEPAIRLASELADRAPGDLNHVYFTLGGSDAVDSTIRFIRYYWNARGRPERDQFISVEQGYHGSSTAGAGLT
ALPAFHAGFGLPFDWQHKIPSHYAYRNPVGEDPQAIIEASLAALKSKVEAIGPELVAAFYVEPIQGSGGVLVPPKGWVKA
MREFCRAHDILFVADEVITGFGRTGPLFACSEEEVVPDFMTTAKGLTSGYVPMGAVFMADHVYETIAEGAGTTAIGHGYT
YSAHPVSAAVGLEVLKLYENGLFDNGVKAGARLMQGLQSLRDHPLVGDVRGRGMLAAVELVVDKSNKTPLPASAEPARRI
FDRAWENGLVIRAFGNGVLGYAPPLCCTETEIDAIVERTRITLDETLEDPDVRRALRV

Sequences:

>Translated_458_residues
MYANSLIELDRAHLIHPVASYRSHEKHGVRVLASAKGATVTDASGKQLIDGFAGLWCVNAGYGHESIVEAAARQMRELPY
ATAYFGLGSEPAIRLASELADRAPGDLNHVYFTLGGSDAVDSTIRFIRYYWNARGRPERDQFISVEQGYHGSSTAGAGLT
ALPAFHAGFGLPFDWQHKIPSHYAYRNPVGEDPQAIIEASLAALKSKVEAIGPELVAAFYVEPIQGSGGVLVPPKGWVKA
MREFCRAHDILFVADEVITGFGRTGPLFACSEEEVVPDFMTTAKGLTSGYVPMGAVFMADHVYETIAEGAGTTAIGHGYT
YSAHPVSAAVGLEVLKLYENGLFDNGVKAGARLMQGLQSLRDHPLVGDVRGRGMLAAVELVVDKSNKTPLPASAEPARRI
FDRAWENGLVIRAFGNGVLGYAPPLCCTETEIDAIVERTRITLDETLEDPDVRRALRV
>Mature_458_residues
MYANSLIELDRAHLIHPVASYRSHEKHGVRVLASAKGATVTDASGKQLIDGFAGLWCVNAGYGHESIVEAAARQMRELPY
ATAYFGLGSEPAIRLASELADRAPGDLNHVYFTLGGSDAVDSTIRFIRYYWNARGRPERDQFISVEQGYHGSSTAGAGLT
ALPAFHAGFGLPFDWQHKIPSHYAYRNPVGEDPQAIIEASLAALKSKVEAIGPELVAAFYVEPIQGSGGVLVPPKGWVKA
MREFCRAHDILFVADEVITGFGRTGPLFACSEEEVVPDFMTTAKGLTSGYVPMGAVFMADHVYETIAEGAGTTAIGHGYT
YSAHPVSAAVGLEVLKLYENGLFDNGVKAGARLMQGLQSLRDHPLVGDVRGRGMLAAVELVVDKSNKTPLPASAEPARRI
FDRAWENGLVIRAFGNGVLGYAPPLCCTETEIDAIVERTRITLDETLEDPDVRRALRV

Specific function: Biotin biosynthesis. [C]

COG id: COG0161

COG function: function code H; Adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI37574042, Length=420, Percent_Identity=29.7619047619048, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI13994255, Length=474, Percent_Identity=27.8481012658228, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI24119277, Length=437, Percent_Identity=28.1464530892449, Blast_Score=152, Evalue=6e-37,
Organism=Homo sapiens, GI226442709, Length=387, Percent_Identity=30.4909560723514, Blast_Score=152, Evalue=6e-37,
Organism=Homo sapiens, GI226442705, Length=416, Percent_Identity=29.8076923076923, Blast_Score=152, Evalue=6e-37,
Organism=Homo sapiens, GI4557809, Length=430, Percent_Identity=28.6046511627907, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI284507298, Length=229, Percent_Identity=36.2445414847162, Blast_Score=124, Evalue=2e-28,
Organism=Escherichia coli, GI1786991, Length=448, Percent_Identity=28.3482142857143, Blast_Score=165, Evalue=4e-42,
Organism=Escherichia coli, GI145693181, Length=409, Percent_Identity=29.8288508557457, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI1789016, Length=439, Percent_Identity=29.8405466970387, Blast_Score=164, Evalue=2e-41,
Organism=Escherichia coli, GI1788044, Length=407, Percent_Identity=29.4840294840295, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1787560, Length=429, Percent_Identity=32.8671328671329, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI1789759, Length=405, Percent_Identity=27.4074074074074, Blast_Score=142, Evalue=6e-35,
Organism=Escherichia coli, GI1786349, Length=338, Percent_Identity=26.6272189349112, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI32564660, Length=421, Percent_Identity=30.6413301662708, Blast_Score=154, Evalue=9e-38,
Organism=Caenorhabditis elegans, GI25144271, Length=412, Percent_Identity=27.9126213592233, Blast_Score=148, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI25144274, Length=231, Percent_Identity=33.7662337662338, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI71992977, Length=409, Percent_Identity=27.3838630806846, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17541228, Length=477, Percent_Identity=25.5765199161426, Blast_Score=94, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6323470, Length=445, Percent_Identity=24.2696629213483, Blast_Score=121, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6324432, Length=405, Percent_Identity=23.2098765432099, Blast_Score=114, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6324386, Length=474, Percent_Identity=24.8945147679325, Blast_Score=113, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6321456, Length=372, Percent_Identity=23.3870967741935, Blast_Score=80, Evalue=5e-16,
Organism=Drosophila melanogaster, GI28574759, Length=435, Percent_Identity=30.5747126436782, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI161085790, Length=432, Percent_Identity=30.787037037037, Blast_Score=155, Evalue=7e-38,
Organism=Drosophila melanogaster, GI21357415, Length=438, Percent_Identity=28.310502283105, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI21356575, Length=446, Percent_Identity=25.7847533632287, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI281366494, Length=382, Percent_Identity=26.9633507853403, Blast_Score=76, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24667139, Length=382, Percent_Identity=26.9633507853403, Blast_Score=76, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24667143, Length=382, Percent_Identity=26.9633507853403, Blast_Score=76, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: 2.6.1.62 [C]

Molecular weight: Translated: 49415; Mature: 49415

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYANSLIELDRAHLIHPVASYRSHEKHGVRVLASAKGATVTDASGKQLIDGFAGLWCVNA
CCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEECCCCHHHHHHHHHHEEEEC
GYGHESIVEAAARQMRELPYATAYFGLGSEPAIRLASELADRAPGDLNHVYFTLGGSDAV
CCCHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCHHH
DSTIRFIRYYWNARGRPERDQFISVEQGYHGSSTAGAGLTALPAFHAGFGLPFDWQHKIP
HHHHHHHHHHHCCCCCCCHHHEEECCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCC
SHYAYRNPVGEDPQAIIEASLAALKSKVEAIGPELVAAFYVEPIQGSGGVLVPPKGWVKA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHEEEECCCCCEEECCHHHHHH
MREFCRAHDILFVADEVITGFGRTGPLFACSEEEVVPDFMTTAKGLTSGYVPMGAVFMAD
HHHHHHHCCEEEEHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHCCCCCCHHHHHHHHH
HVYETIAEGAGTTAIGHGYTYSAHPVSAAVGLEVLKLYENGLFDNGVKAGARLMQGLQSL
HHHHHHHCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
RDHPLVGDVRGRGMLAAVELVVDKSNKTPLPASAEPARRIFDRAWENGLVIRAFGNGVLG
HCCCCEECCCCCCHHEEEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCC
YAPPLCCTETEIDAIVERTRITLDETLEDPDVRRALRV
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHCC
>Mature Secondary Structure
MYANSLIELDRAHLIHPVASYRSHEKHGVRVLASAKGATVTDASGKQLIDGFAGLWCVNA
CCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEECCCCHHHHHHHHHHEEEEC
GYGHESIVEAAARQMRELPYATAYFGLGSEPAIRLASELADRAPGDLNHVYFTLGGSDAV
CCCHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCHHH
DSTIRFIRYYWNARGRPERDQFISVEQGYHGSSTAGAGLTALPAFHAGFGLPFDWQHKIP
HHHHHHHHHHHCCCCCCCHHHEEECCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCC
SHYAYRNPVGEDPQAIIEASLAALKSKVEAIGPELVAAFYVEPIQGSGGVLVPPKGWVKA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHEEEECCCCCEEECCHHHHHH
MREFCRAHDILFVADEVITGFGRTGPLFACSEEEVVPDFMTTAKGLTSGYVPMGAVFMAD
HHHHHHHCCEEEEHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHCCCCCCHHHHHHHHH
HVYETIAEGAGTTAIGHGYTYSAHPVSAAVGLEVLKLYENGLFDNGVKAGARLMQGLQSL
HHHHHHHCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
RDHPLVGDVRGRGMLAAVELVVDKSNKTPLPASAEPARRIFDRAWENGLVIRAFGNGVLG
HCCCCEECCCCCCHHEEEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCC
YAPPLCCTETEIDAIVERTRITLDETLEDPDVRRALRV
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Pyridoxal Phosphate. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 8-Amino-7-oxononanoate; S-Adenosyl-L-methionine [C]

Specific reaction: 8-Amino-7-oxononanoate + S-Adenosyl-L-methionine <==> S-Adenosyl-4-methylthio-2-oxobutanoate + 7,8-Diaminononanoate [C]

General reaction: Amino group transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8796346; 9163424 [H]