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The map label for this gene is nwsB

Identifier: 86360953

GI number: 86360953

Start: 238234

End: 238875

Strand: Direct

Name: nwsB

Synonym: RHE_PF00223

Alternate gene names: 86360953

Gene position: 238234-238875 (Clockwise)

Preceding gene: 86360952

Following gene: 86360954

Centisome position: 37.08

GC content: 61.06

Gene sequence:

>642_bases
ATGACGACCGATGACCATATCGTCTTCATCGTAGATGATGATGAACGCATCCGGGAGGCTCTCGGCGAGCTGCTGGACTC
GCATGGCATACGCGCCATCGCCTTCGAGTCGGCCTGCGACTATGTGCAAGCGGATAAGCCGGATGTTCCGGCCTGCCTTA
TCCTCGATATCGAACTGCCCGATATCAACGGCCTCGACCTACAGCGGCAGATCGCCGACGGCGATCATCCGCCGATCGTC
TTCGTCACCGGACATGGCGACATCCCCTCCTCCGTGCGCGCCATCAAGCGCGGCGCGGTCGATTTTCTCACCAAGCCCTT
CAGCGATGAGCACCTTATGGCGGCGATCCAGGCGGCGATCGCCCAGGATCGGGAAAAGAGGACCGAGCGCGCCGAACTCG
GCATGCTGAAGCAGCACTATCTCGACCTGACACCGCGTGAACGCGAGGTGTTGCCGCTCGTCGTCAGCGGCCTTCTCAAC
AAGCAGGCAGCGGCAGAACTGGGGATCAGCGAAGTCACGCTGCAGATCCACAGAAGAAACGTGATGCAGAAGATGGCGGC
GGCATCGCTCGCCGACCTCGTGCGCATCGCGGAGAAACTGGAAATACCGATAACCCACTCGCGCCGGGTGGGAGGGAATT
GA

Upstream 100 bases:

>100_bases
CGATCGTCGAGCTGCATGGCGGACGATTGTGGGCGGAGAAGAACCAACCGCACGGAGCGACGTTGATCTTTTCATTGCCG
GTTGAAACGAAGGCGGCGTC

Downstream 100 bases:

>100_bases
ACATGAACAAGACAAGACATGTCGTGGCAATTGTCGACGACGATCCAAGACTCCTTGAATCGATGAGCGACCTTCTGGAA
TCGGCGGGCTATGTCGCCCG

Product: two-component response regulator protein (nodW mutant supressor protein)

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 213; Mature: 212

Protein sequence:

>213_residues
MTTDDHIVFIVDDDERIREALGELLDSHGIRAIAFESACDYVQADKPDVPACLILDIELPDINGLDLQRQIADGDHPPIV
FVTGHGDIPSSVRAIKRGAVDFLTKPFSDEHLMAAIQAAIAQDREKRTERAELGMLKQHYLDLTPREREVLPLVVSGLLN
KQAAAELGISEVTLQIHRRNVMQKMAAASLADLVRIAEKLEIPITHSRRVGGN

Sequences:

>Translated_213_residues
MTTDDHIVFIVDDDERIREALGELLDSHGIRAIAFESACDYVQADKPDVPACLILDIELPDINGLDLQRQIADGDHPPIV
FVTGHGDIPSSVRAIKRGAVDFLTKPFSDEHLMAAIQAAIAQDREKRTERAELGMLKQHYLDLTPREREVLPLVVSGLLN
KQAAAELGISEVTLQIHRRNVMQKMAAASLADLVRIAEKLEIPITHSRRVGGN
>Mature_212_residues
TTDDHIVFIVDDDERIREALGELLDSHGIRAIAFESACDYVQADKPDVPACLILDIELPDINGLDLQRQIADGDHPPIVF
VTGHGDIPSSVRAIKRGAVDFLTKPFSDEHLMAAIQAAIAQDREKRTERAELGMLKQHYLDLTPREREVLPLVVSGLLNK
QAAAELGISEVTLQIHRRNVMQKMAAASLADLVRIAEKLEIPITHSRRVGGN

Specific function: Member of the two-component regulatory system nodV/nodW probably involved in the regulation of the transcription of genes involved in the nodulation process [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=126, Percent_Identity=34.9206349206349, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI1790102, Length=192, Percent_Identity=31.25, Blast_Score=68, Evalue=4e-13,
Organism=Escherichia coli, GI1790860, Length=123, Percent_Identity=29.2682926829268, Blast_Score=62, Evalue=2e-11,
Organism=Escherichia coli, GI1788222, Length=190, Percent_Identity=23.6842105263158, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR011990
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23535; Mature: 23404

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTDDHIVFIVDDDERIREALGELLDSHGIRAIAFESACDYVQADKPDVPACLILDIELP
CCCCCEEEEEECCCHHHHHHHHHHHHHCCCEEEEHHHHHHHHCCCCCCCCEEEEEEEECC
DINGLDLQRQIADGDHPPIVFVTGHGDIPSSVRAIKRGAVDFLTKPFSDEHLMAAIQAAI
CCCCCCHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
AQDREKRTERAELGMLKQHYLDLTPREREVLPLVVSGLLNKQAAAELGISEVTLQIHRRN
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
VMQKMAAASLADLVRIAEKLEIPITHSRRVGGN
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
TTDDHIVFIVDDDERIREALGELLDSHGIRAIAFESACDYVQADKPDVPACLILDIELP
CCCCEEEEEECCCHHHHHHHHHHHHHCCCEEEEHHHHHHHHCCCCCCCCEEEEEEEECC
DINGLDLQRQIADGDHPPIVFVTGHGDIPSSVRAIKRGAVDFLTKPFSDEHLMAAIQAAI
CCCCCCHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
AQDREKRTERAELGMLKQHYLDLTPREREVLPLVVSGLLNKQAAAELGISEVTLQIHRRN
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
VMQKMAAASLADLVRIAEKLEIPITHSRRVGGN
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2320582; 11157954; 12597275 [H]