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The map label for this gene is fixJ [H]

Identifier: 86360954

GI number: 86360954

Start: 238878

End: 239255

Strand: Direct

Name: fixJ [H]

Synonym: RHE_PF00224

Alternate gene names: 86360954

Gene position: 238878-239255 (Clockwise)

Preceding gene: 86360953

Following gene: 86360955

Centisome position: 37.18

GC content: 60.05

Gene sequence:

>378_bases
ATGAACAAGACAAGACATGTCGTGGCAATTGTCGACGACGATCCAAGACTCCTTGAATCGATGAGCGACCTTCTGGAATC
GGCGGGCTATGTCGCCCGCGCCTTCTCGTCGGCAGGCTCGCTGCTCGTCAACGGGCTGTCGGACCTGGATCTGCTCATCA
CCGACATCGGAATGCCCGGCATAGACGGCTTCGAACTTCGTGACCTCGTCAGGAAATCACGTCCGGAGCTGCCGGTTTTC
CTGATCACGGGCCGCCACGAGATCGCGGATCAGGGCCGCGCTCAAGGCGCCGGCGGATTCTTCCGCAAGCCATTCGATGC
CCAGGCCCTGATCGCGGCCATCGCCAATGCCTTACACAAATCGAGAGATGGAGGGTGA

Upstream 100 bases:

>100_bases
TGCAGAAGATGGCGGCGGCATCGCTCGCCGACCTCGTGCGCATCGCGGAGAAACTGGAAATACCGATAACCCACTCGCGC
CGGGTGGGAGGGAATTGAAC

Downstream 100 bases:

>100_bases
CATGAGGGTTGACCAGCCGTTCGTTCGTAGATCGGCGCCTTTGTCAGCGCAGCGTGAACGTGAAGAGGATCAGCCGCTCG
TCATGATCGTGGACGACGAC

Product: putative two-component response regulator protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 125; Mature: 125

Protein sequence:

>125_residues
MNKTRHVVAIVDDDPRLLESMSDLLESAGYVARAFSSAGSLLVNGLSDLDLLITDIGMPGIDGFELRDLVRKSRPELPVF
LITGRHEIADQGRAQGAGGFFRKPFDAQALIAAIANALHKSRDGG

Sequences:

>Translated_125_residues
MNKTRHVVAIVDDDPRLLESMSDLLESAGYVARAFSSAGSLLVNGLSDLDLLITDIGMPGIDGFELRDLVRKSRPELPVF
LITGRHEIADQGRAQGAGGFFRKPFDAQALIAAIANALHKSRDGG
>Mature_125_residues
MNKTRHVVAIVDDDPRLLESMSDLLESAGYVARAFSSAGSLLVNGLSDLDLLITDIGMPGIDGFELRDLVRKSRPELPVF
LITGRHEIADQGRAQGAGGFFRKPFDAQALIAAIANALHKSRDGG

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788905, Length=123, Percent_Identity=39.0243902439024, Blast_Score=62, Evalue=9e-12,
Organism=Escherichia coli, GI1790299, Length=117, Percent_Identity=31.6239316239316, Blast_Score=60, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 13416; Mature: 13416

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKTRHVVAIVDDDPRLLESMSDLLESAGYVARAFSSAGSLLVNGLSDLDLLITDIGMPG
CCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCC
IDGFELRDLVRKSRPELPVFLITGRHEIADQGRAQGAGGFFRKPFDAQALIAAIANALHK
CCCHHHHHHHHHCCCCCCEEEEECCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
SRDGG
CCCCC
>Mature Secondary Structure
MNKTRHVVAIVDDDPRLLESMSDLLESAGYVARAFSSAGSLLVNGLSDLDLLITDIGMPG
CCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCC
IDGFELRDLVRKSRPELPVFLITGRHEIADQGRAQGAGGFFRKPFDAQALIAAIANALHK
CCCHHHHHHHHHCCCCCCEEEEECCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
SRDGG
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2046550 [H]