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The map label for this gene is cspA [C]

Identifier: 86360861

GI number: 86360861

Start: 138429

End: 138638

Strand: Direct

Name: cspA [C]

Synonym: RHE_PF00130

Alternate gene names: 86360861

Gene position: 138429-138638 (Clockwise)

Preceding gene: 86360860

Following gene: 86360862

Centisome position: 21.54

GC content: 53.33

Gene sequence:

>210_bases
ATGACCATTGGCACAGTAAAATGGTTCAACTCCACCAAAGGCTTCGGCTTCATACAACCTGACGACGGGAGTGCCGACGT
CTTCGTTCACATCTCTGCCGTTGAACGCGCGGGAATGAGTTCAATCGCTGAAGGTCAGAAGCTCGGCTTTCAATTGGAGC
ATGACCGTAAGTCCGGCAAAATGTCTGCGGGCCAGCTCCAAGCGGCCTAA

Upstream 100 bases:

>100_bases
TGCGCTCTGCGCCTCTTGATCAACTCTCCCTTCAAAAGTCGAAACACCGCCGCGCGCCGCTCGGCGGGCAATTAAATGTA
TGCCGATGAAAGGGTTTATC

Downstream 100 bases:

>100_bases
AAAGCTCCTGTAGCAGCTGCGACCACGGACGTACTGGCGTGGCTGGCAAGGTCAGTTGAAGGCCAGGCAGATGTCTGGCC
TTTTTCATTCAAGAAGACCG

Product: putative cold shock protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 69; Mature: 68

Protein sequence:

>69_residues
MTIGTVKWFNSTKGFGFIQPDDGSADVFVHISAVERAGMSSIAEGQKLGFQLEHDRKSGKMSAGQLQAA

Sequences:

>Translated_69_residues
MTIGTVKWFNSTKGFGFIQPDDGSADVFVHISAVERAGMSSIAEGQKLGFQLEHDRKSGKMSAGQLQAA
>Mature_68_residues
TIGTVKWFNSTKGFGFIQPDDGSADVFVHISAVERAGMSSIAEGQKLGFQLEHDRKSGKMSAGQLQAA

Specific function: Binds To And Stimulates The Transcription Of The CCAAT- Containing, Cold-Shock-Inducible Promoters Of The H-Ns And Gyra Proteins. Binds Also To The Inverted Repeat 5'-ATTGG-3'. [C]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1789979, Length=54, Percent_Identity=61.1111111111111, Blast_Score=73, Evalue=3e-15,
Organism=Escherichia coli, GI1786841, Length=54, Percent_Identity=55.5555555555556, Blast_Score=72, Evalue=8e-15,
Organism=Escherichia coli, GI1788126, Length=54, Percent_Identity=55.5555555555556, Blast_Score=69, Evalue=6e-14,
Organism=Escherichia coli, GI2367114, Length=54, Percent_Identity=51.8518518518518, Blast_Score=67, Evalue=2e-13,
Organism=Escherichia coli, GI1787839, Length=50, Percent_Identity=58, Blast_Score=66, Evalue=4e-13,
Organism=Escherichia coli, GI1787834, Length=54, Percent_Identity=51.8518518518518, Blast_Score=64, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 7373; Mature: 7242

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIGTVKWFNSTKGFGFIQPDDGSADVFVHISAVERAGMSSIAEGQKLGFQLEHDRKSGK
CCCCCEEECCCCCCCEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEECCCCCCC
MSAGQLQAA
CCCCEEECC
>Mature Secondary Structure 
TIGTVKWFNSTKGFGFIQPDDGSADVFVHISAVERAGMSSIAEGQKLGFQLEHDRKSGK
CCCCEEECCCCCCCEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEECCCCCCC
MSAGQLQAA
CCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]