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The map label for this gene is phbAf

Identifier: 86360748

GI number: 86360748

Start: 13624

End: 14829

Strand: Direct

Name: phbAf

Synonym: RHE_PF00014

Alternate gene names: 86360748

Gene position: 13624-14829 (Clockwise)

Preceding gene: 86360747

Following gene: 86360750

Centisome position: 2.12

GC content: 66.83

Gene sequence:

>1206_bases
ATGATCTTGTCCTACGCCTATCAGCCTGACGAGGATTGGCAGCCGGTCGTGGTCGCCGCCTACCGCACACCGATCGGCCG
GGCCTTCGGCTCGCTGGCTACGGTTGCGGCCGAAGACCTGCTTGCGCCCATCATCCGCCGCATCCTTGCAGAAACGGGCG
TCGGGCCCGACGCCATCGACGACGTGCTGGTCGGCAATGCCGCCGGCGGCGGCGGCAATATCGGCCGGCTGGCGGCGCTG
ACTGCCGGCCTGCCCATGGCCGTTCCTGGTGTTGCGATCGACCGGCAATGTGGTTCGGGCCTCGAAGCAATCATCATGGC
CGCCCGGCTGATCCAGGCGAAGGCCGGTTCGTGCTTCCTTGCCGGCGGCGTCGAAAGCGTCAGCACCGCGCCATGGCGCG
TCGAAAGACCGAAAGCCAATGGTGCCGTGCCGCGCTTTTACGGCCGCGCACGGTTTTCGCCTGAAACGATCGGCGACCCC
GAGATGGGGGTAGCCGCTGAAAACGTCGCCCGTCAATTCGGCATCTCGCGCCAGCGGCAGGATGAATTCGCCCTTCGCAG
CCATCGGCTCGCTGTCGCAGCGGCCGAAGCGGGCCTCTTCCGCCCCGAGATCGTCGAGATCACCACCATGCGTGGCCCGG
TAGAGTGGGACGAATGTCCGCGCCCGACCACCTCGCCCGAGGCGCTCGCGAACCTGAAGCCTGTCTTCTTGGCCGATGGC
TCGGTGACGGCAGGCAATGCTTGCCCTGTGAATGACGGCGCCTGCCTGGTGCTCGTCATGAGCCGCGGCATGGCCAGAAA
CCTCGGCATAGAAAAGGGCCTCGCCTTCATCGACAGCGCCGCCGCCGGCGTCGATCCAAACTTGCTCGGAATCGGACCGG
TCGCCGCGACCGGCAAGCTCATGCAGCGCCAGCCCGACCTCTCGCTTTCCGCAATCGACGTCATCGAATTCAACGAGGCT
TTCGCCGCCCAGGTCCTGGCGTCGCTCGATCAGCTGGAGATCCCCGCCAACGCGGTCAACAAAGAAGGCGGCGCTATCGC
GCTCGGCCACCCCTTCGGCGCCTCCGGCGCTATCCTGGTCACCCGGCTCTACGGTCAGCTCATCCGCGGCGACGGCTGGA
CACCACGCGCAACCGGCCTCGCCATGATCGGCATCGGCGGCGGCATCGGGCTCACCGCGTTGTTCGAGGCTGTCCGCCTT
TTCTGA

Upstream 100 bases:

>100_bases
TTCAAGATTCCCAGGCAGTTCTACCATATCGGCTCCTGGCCGATGACGAGCAGCGGCAAGATCGCGCGCGGCCAGGTGGA
AGCATCGGTCATTTCGGGAG

Downstream 100 bases:

>100_bases
GAAGACCGCCATTCAGGCACGTGCGCTGCCGGCTTCAAGCCTCTTGTGATCTGGCTCGGTCGCTATTGGAAACCAAGGCG
GCTCCGGTGCGGAGAGGGCA

Product: acetyl-CoA acetyltransferase (beta-ketothiolase) protein

Products: Acetoacetyl-CoA; Coenzyme A [C]

Alternate protein names: NA

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MILSYAYQPDEDWQPVVVAAYRTPIGRAFGSLATVAAEDLLAPIIRRILAETGVGPDAIDDVLVGNAAGGGGNIGRLAAL
TAGLPMAVPGVAIDRQCGSGLEAIIMAARLIQAKAGSCFLAGGVESVSTAPWRVERPKANGAVPRFYGRARFSPETIGDP
EMGVAAENVARQFGISRQRQDEFALRSHRLAVAAAEAGLFRPEIVEITTMRGPVEWDECPRPTTSPEALANLKPVFLADG
SVTAGNACPVNDGACLVLVMSRGMARNLGIEKGLAFIDSAAAGVDPNLLGIGPVAATGKLMQRQPDLSLSAIDVIEFNEA
FAAQVLASLDQLEIPANAVNKEGGAIALGHPFGASGAILVTRLYGQLIRGDGWTPRATGLAMIGIGGGIGLTALFEAVRL
F

Sequences:

>Translated_401_residues
MILSYAYQPDEDWQPVVVAAYRTPIGRAFGSLATVAAEDLLAPIIRRILAETGVGPDAIDDVLVGNAAGGGGNIGRLAAL
TAGLPMAVPGVAIDRQCGSGLEAIIMAARLIQAKAGSCFLAGGVESVSTAPWRVERPKANGAVPRFYGRARFSPETIGDP
EMGVAAENVARQFGISRQRQDEFALRSHRLAVAAAEAGLFRPEIVEITTMRGPVEWDECPRPTTSPEALANLKPVFLADG
SVTAGNACPVNDGACLVLVMSRGMARNLGIEKGLAFIDSAAAGVDPNLLGIGPVAATGKLMQRQPDLSLSAIDVIEFNEA
FAAQVLASLDQLEIPANAVNKEGGAIALGHPFGASGAILVTRLYGQLIRGDGWTPRATGLAMIGIGGGIGLTALFEAVRL
F
>Mature_401_residues
MILSYAYQPDEDWQPVVVAAYRTPIGRAFGSLATVAAEDLLAPIIRRILAETGVGPDAIDDVLVGNAAGGGGNIGRLAAL
TAGLPMAVPGVAIDRQCGSGLEAIIMAARLIQAKAGSCFLAGGVESVSTAPWRVERPKANGAVPRFYGRARFSPETIGDP
EMGVAAENVARQFGISRQRQDEFALRSHRLAVAAAEAGLFRPEIVEITTMRGPVEWDECPRPTTSPEALANLKPVFLADG
SVTAGNACPVNDGACLVLVMSRGMARNLGIEKGLAFIDSAAAGVDPNLLGIGPVAATGKLMQRQPDLSLSAIDVIEFNEA
FAAQVLASLDQLEIPANAVNKEGGAIALGHPFGASGAILVTRLYGQLIRGDGWTPRATGLAMIGIGGGIGLTALFEAVRL
F

Specific function: Unknown

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI4501853, Length=393, Percent_Identity=37.6590330788804, Blast_Score=216, Evalue=2e-56,
Organism=Homo sapiens, GI148539872, Length=399, Percent_Identity=37.34335839599, Blast_Score=210, Evalue=2e-54,
Organism=Homo sapiens, GI167614485, Length=388, Percent_Identity=36.340206185567, Blast_Score=200, Evalue=2e-51,
Organism=Homo sapiens, GI4557237, Length=404, Percent_Identity=34.6534653465347, Blast_Score=183, Evalue=3e-46,
Organism=Homo sapiens, GI4504327, Length=423, Percent_Identity=30.4964539007092, Blast_Score=145, Evalue=5e-35,
Organism=Homo sapiens, GI194353979, Length=384, Percent_Identity=27.8645833333333, Blast_Score=118, Evalue=1e-26,
Organism=Escherichia coli, GI87082165, Length=396, Percent_Identity=40.6565656565657, Blast_Score=237, Evalue=8e-64,
Organism=Escherichia coli, GI1787663, Length=394, Percent_Identity=41.8781725888325, Blast_Score=234, Evalue=1e-62,
Organism=Escherichia coli, GI1788554, Length=395, Percent_Identity=42.0253164556962, Blast_Score=229, Evalue=2e-61,
Organism=Escherichia coli, GI48994986, Length=399, Percent_Identity=39.8496240601504, Blast_Score=214, Evalue=6e-57,
Organism=Escherichia coli, GI1788683, Length=401, Percent_Identity=28.1795511221945, Blast_Score=132, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI133906874, Length=394, Percent_Identity=37.5634517766497, Blast_Score=222, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI25147385, Length=388, Percent_Identity=33.2474226804124, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17535921, Length=388, Percent_Identity=33.2474226804124, Blast_Score=163, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17551802, Length=428, Percent_Identity=30.1401869158879, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17535917, Length=369, Percent_Identity=28.4552845528455, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6322031, Length=389, Percent_Identity=36.5038560411311, Blast_Score=196, Evalue=4e-51,
Organism=Saccharomyces cerevisiae, GI6325229, Length=399, Percent_Identity=32.3308270676692, Blast_Score=150, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24655093, Length=400, Percent_Identity=37.25, Blast_Score=214, Evalue=8e-56,
Organism=Drosophila melanogaster, GI17648125, Length=394, Percent_Identity=34.5177664974619, Blast_Score=189, Evalue=4e-48,
Organism=Drosophila melanogaster, GI24640423, Length=403, Percent_Identity=34.7394540942928, Blast_Score=177, Evalue=2e-44,
Organism=Drosophila melanogaster, GI17137578, Length=424, Percent_Identity=30.6603773584906, Blast_Score=149, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: 2.3.1.9 [C]

Molecular weight: Translated: 41558; Mature: 41558

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00737 THIOLASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILSYAYQPDEDWQPVVVAAYRTPIGRAFGSLATVAAEDLLAPIIRRILAETGVGPDAID
CEEEEECCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
DVLVGNAAGGGGNIGRLAALTAGLPMAVPGVAIDRQCGSGLEAIIMAARLIQAKAGSCFL
HHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCEECCHHCCCHHHHHHHHHHHHHCCCCEEE
AGGVESVSTAPWRVERPKANGAVPRFYGRARFSPETIGDPEMGVAAENVARQFGISRQRQ
ECCCCCCCCCCCEEECCCCCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHH
DEFALRSHRLAVAAAEAGLFRPEIVEITTMRGPVEWDECPRPTTSPEALANLKPVFLADG
HHHHHHHHHHEEEEHHCCCCCCCEEEEEECCCCCCHHHCCCCCCCHHHHHCCCCEEEECC
SVTAGNACPVNDGACLVLVMSRGMARNLGIEKGLAFIDSAAAGVDPNLLGIGPVAATGKL
CEECCCCCCCCCCCEEEEEECCCHHHHCCHHHHHHHHHHHHCCCCCCEEECCCHHHHHHH
MQRQPDLSLSAIDVIEFNEAFAAQVLASLDQLEIPANAVNKEGGAIALGHPFGASGAILV
HHCCCCCCEEEEEEEECCHHHHHHHHHHHHHHCCCHHHCCCCCCEEEECCCCCCCCHHHH
TRLYGQLIRGDGWTPRATGLAMIGIGGGIGLTALFEAVRLF
HHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MILSYAYQPDEDWQPVVVAAYRTPIGRAFGSLATVAAEDLLAPIIRRILAETGVGPDAID
CEEEEECCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
DVLVGNAAGGGGNIGRLAALTAGLPMAVPGVAIDRQCGSGLEAIIMAARLIQAKAGSCFL
HHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCEECCHHCCCHHHHHHHHHHHHHCCCCEEE
AGGVESVSTAPWRVERPKANGAVPRFYGRARFSPETIGDPEMGVAAENVARQFGISRQRQ
ECCCCCCCCCCCEEECCCCCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHH
DEFALRSHRLAVAAAEAGLFRPEIVEITTMRGPVEWDECPRPTTSPEALANLKPVFLADG
HHHHHHHHHHEEEEHHCCCCCCCEEEEEECCCCCCHHHCCCCCCCHHHHHCCCCEEEECC
SVTAGNACPVNDGACLVLVMSRGMARNLGIEKGLAFIDSAAAGVDPNLLGIGPVAATGKL
CEECCCCCCCCCCCEEEEEECCCHHHHCCHHHHHHHHHHHHCCCCCCEEECCCHHHHHHH
MQRQPDLSLSAIDVIEFNEAFAAQVLASLDQLEIPANAVNKEGGAIALGHPFGASGAILV
HHCCCCCCEEEEEEEECCHHHHHHHHHHHHHHCCCHHHCCCCCCEEEECCCCCCCCHHHH
TRLYGQLIRGDGWTPRATGLAMIGIGGGIGLTALFEAVRLF
HHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Acetyl-CoA [C]

Specific reaction: (2) Acetyl-CoA <==> Acetoacetyl-CoA + Coenzyme A [C]

General reaction: Acyl group transfer; Condensation; Thiolytic cleavage [C]

Inhibitor: Acetoacetyl-CoA; Borohydride; Iodoacetamide; N-Ethyl maleimide; p-Chloromercuribenzoate; Sulfhydryl reagents [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10708580 [H]